HAL Open Archives: https://hal.utc.fr/GEC/en
Merlier F., Octave S., Tse Sum Bui B., Thomasset B. Evaluation of Performance and Validity Limits of Gas Chromatography electron ionization – Orbitrap Detector for fatty acid methyl esters analyses . Rapid Commun Mass Spectrom, 2019, in press,.
Medina Rangel P., Moroni E., Merlier F., Gheber L., Vago R., Tse Sum Bui B., Haupt K. Chemical Antibody Mimics Inhibit Cadherin-Mediated Cell-Cell Adhesion: A Promising Strategy for Cancer Therapy. Angewandte Chemie, 2019, In press ,.
Martins-noguerol R., Moreno-pérez Aj., Acket S., Makni S., Garcés R., Troncoso-ponce A., Salas Jj., Thomasset B., Martínez-force E. Lipidomic Analysis of Plastidial Octanoyltransferase Mutants of Arabidopsis thaliana . Metabolites , 2019, 9(10) ,209 .
Rondeau M., Esmaeel Q., Crouzet J., Blin Pauline., Gosselin I., Sarazin C., Pernes M., Beugrand J., Wisniewski-dyé F., Vial L., Faure D. Clément C., Ait Barka E., Jacquard C. Sanchez L. Biofilm constructing variants of Paraburkholderia phytofirmans PsJN outcompete the wild-type form in free-living and static conditions but not in planta . Applied and Environmental Microbilogu , 2019, 85 ,e02670-18 .
Araya-farias M., Husson E., Saavedra-torrico J., Gérard D., Roulard R., Gosselin I., Rakotoarivonina H., Lambertyn V. Rémond C. Sarazin C. Wheat Bran Pretreatment by Room Temperature Ionic Liquid-Water Mixture: Optimization of Process Conditions by PLS-Surface Response Design . Fontiers in Chemistry , 2019, 7 ,585.
Husson E., Hulin L., Hadad C., Boughanmi C., Stevanovic T., Sarazin C. Acidic Ionic Liquid as Both Solvent and Catalyst for Fast Chemical Esterification of Industrial Lignins: Performances and Regioselectivity . Fontiers in Chemistry , 2019, 7 ,578. ABSTRACT:
Alayoubi R., Mehmood N., Husson E., Kouzayha A., Tabcheh M., Chaveriat L, Sarazin C., Gosselin I. Low temperature ionic liquid pretreatment of lignocellulosic biomass to enhance bioethanol yield . Renewable Energy , 2019, 145 ,1808-1816 .
Mier A., Nestora S., Medina Rangel P., Rossez Y., Haupt K., Tse Sum Bui B. Cytocompatibility of Moleculary Imprinted Polymers for Deodorants : Evaluation on Human Keratinocytes and Axillary-Hosted Bacteria . ACS Appl. Mater. Interfaces , 2019, 2 ,3439-3447 ABSTRACT: https://doi.org/10.1021/acsabm.9b00388
Le Priol L., Dagmey A., Morandat S., Saleh K., El Kirat K., Nesterenko A. Comparative study of plant protein extracts as wall materials for the improvement of the oxidative stability of sunflower oil by microencapsulation. Food Hydrocolloids, 2019, 95,105-115.
Monnier N., Furlan A-l., Buchoux S., Deleu M., Dauchez M., Rippa S., Sarazin C. Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies" has been published in IJMS as part of the Special Issue SAR "Out of the Box. Int. J. Mol. Sci., 2019, 20,1009.
Medina Rangel P., Caclef S., Xu J., Panagiotopoulou M., Kovensky J., Tse Sum Bui B., Haupt K. Solid-phase synthesis of moleculary imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans. Scientific Reports, 2019, 9,3923.
Fahs Z., Rossez Y., Guénin S., Gutierrez L., Thomasset B., & Perrin, Y. Cloning and molecular characterization of three lysophosphatidic acid acyltransferases expressed in flax seeds. Plant Science, 2019, 280,41-50.
Xu J., Merlier F., Avalle B., Veillard V., Debré P., Haupt K., Tse Sum Bui B. Molecularly Imprinted Polymer Nanoparticles as Potential Synthetic Antibodies for Immunoprotection against HIV . ACS Appl. Mater. Interfaces, 2019, 11,9824-9831. ABSTRACT: We describe the preparation and characterization of synthetic antibodies based on molecularly imprinted polymer nanoparticles (MIP-NPs) for the recognition and binding of the highly conserved and specific peptide motif SWSNKS (3S), an epitope of the envelope glycoprotein 41 (gp41) of human immunodeficiency virus type 1 (HIV-1). This motif is implicated in the decline of CD4+ T cells and leads to the deterioration of the immune system during HIV infection. Therefore, the development of MIP-NPs that can target and block the 3S peptide to prevent subsequent cascade interactions directed toward the killing of CD4+ T cells is of prime importance. Because most antibodies recognize their protein antigen via a conformational or structured epitope (as opposed to a linear epitope commonly used for molecular imprinting), we employed protein molecular modeling to design our template epitope so that it mimics the three-dimensional structure fold of 3S in gp41. The resulting template peptide corresponds to a cyclic structure composed of CGSWSNKSC, with the 3S motif well orientated for imprinting. MIP-NPs with a size of 65 nm were obtained by solid-phase synthesis and were water-soluble. They were prepared by a judicious combination of multiple functional monomers affording hydrogen bonding, ionic, π–π, and hydrophobic interactions, conferring high affinity and selectivity toward both the cyclic peptide and the whole gp41 protein. These results suggest that our MIPs could potentially be used for blocking the function of the 3S motif on the virus.
Ksouda G., Sellimi S., Merlier F., Facimaigne-cordin A., Thomasset B., Nasri M., Hajji M. Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive . Food Chemistry, 2019, 288,47-56. ABSTRACT: The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage. The GC–HRMS analysis of PSEO showed that anethole, pseudoisoeugenol and p-anisaldehyde were the main components. The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential. Additionally, PSEO displayed potent antibacterial activity against 3 Gram-positive and 3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL). The acute toxicity of PSEO was determined using mice model (LD50 = 976.2 mg/kg). The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.
Czerwic Q., Idrissitaghki A., Imatoukene N., Nonus M., Thomasset B., Nicaud J-m., Rossignol T. Optimization of cyclopropane fatty acids production in Yarrowia lipolytica. Yeast, 2019, 36,143-151. ABSTRACT: Cyclopropane fatty acids, which can be simply converted to methylated fatty acids, are good unusual fatty acid candidates for long‐term resistance to oxidization and low‐temperature fluidity useful for oleochemistry and biofuels. Cyclopropane fatty acids are present in low amounts in plants or bacteria. In order to develop a process for large‐scale biolipid production, we expressed 10 cyclopropane fatty acid synthases from various organisms in the oleaginous yeast Yarrowia lipolytica, a model yeast for lipid metabolism and naturally capable of producing large amounts of lipids. The Escherichia coli cyclopropane fatty acid synthase expression in Y. lipolytica allows the production of two classes of cyclopropane fatty acids, a C17:0 cyclopropanated form and a C19:0 cyclopropanated form, whereas others produce only the C17:0 form. Expression optimization and fed‐batch fermentation set‐up enable us to reach a specific productivity of 0.032 g·L−1·hr−1 with a genetically modified strain containing cyclopropane fatty acid up to 45% of the total lipid content corresponding to a titre of 2.3 ± 0.2 g/L and a yield of 56.2 ± 4.4 mg/g.
Socrier L., Quero A., Verdu M., Song Y., Milinie R., Mathiron D., Pilard S., Mesnard F., Monrandat S. Flax phenolic compounds as inhibitors of lipid oxidation: Elucidation of their mechanisms of action. Food Chemistry, 2019, 274,651-658.
Furlan A., Buchoux S., Miao Y., Banchet V., Leteve M., Lambertyn V. Michel J., Sarazin C., Bonnet V Nanoparticles based on lipidyl-?-cyclodextrins: synthesis, characterization, and experimental and computational biophysical studies for encapsulation of atazanavir. New journal of Chemistry, 2018, 14,112. ABSTRACT: Amphiphilic cyclodextrins were synthesized from permethylated ?CD with the aim of forming nanoparticles (NPs) that would encapsulate specific molecules (e.g. drugs) which could enhance their otherwise poor bioavailability. By grafting different fatty acids, four amphiphilic CDs were obtained. The self-assembling properties of three of these compounds were evaluated demonstrating micromolar critical aggregation concentration (CAC). Additionally, the stability of these nanoparticles was studied revealing that the compounds with C18 chains could be stored at 4
Bernard C., Acket S., Rossez Y ., Fernandez O., Berton T., Gibon Y., Cabasson C. Untargeted analysis of semipolar compounds by LC-MS and targeted analysis of fatty acids by GC-MS/GC-FID: From plant cultivation to extract preparation. Methods Molec. Biol., 2018, 42,20171-20179. ABSTRACT: The way plants are grown and samples are harvested, prepared, and extracted has a profound impact on the output of a metabolomics experiment. In this chapter, we detail the experimental procedures from plant cultivation to extract preparation, in order to avoid difficulties that could result in contamination or undesired changes of the analytes. Two plant organs are mentioned as examples: tomato fruits (Solanum lycopersicum) and flax seeds (Linum usitatissimum). Extractions designed for the untargeted analysis of semipolar compounds by liquid chromatography
Monnier M., Furlan A., Botcazon C., Dahi A., Mongelard G., Cordelier S., Clement C., Dorey S., Sarazin C., Rippa S. Rhamnolipids from Pseudomonas aeruginosa are elicitors triggering Brassica napus protection against Botrytis cinerea without physiological disorders. Frontiers in Plant Science, 2018, 1778,101-124. ABSTRACT: Rhamnolipids (RLs) are amphiphilic molecules naturally produced by some bacteria with a large range of biological activities. Although some studies report their potential interest in plant protection, evaluation of their effects and efficiency on annual crops of worldwide agronomic interest is lacking. The main objective of this work was to investigate their elicitor and protective activities on rapeseed crop species while evaluating their physiological effects. Here we report that RLs from Pseudomonas aeruginosa secretome trigger an effective protection of Brassica napus foliar tissues toward the fungus Botrytis cinerea involving the combination of plant defense activation and direct antimicrobial properties. We demonstrated their ability to activate canonical B. napus defense responses including reactive oxygen species production, expression of defense genes, along with callose deposits and stomatal closure as efficient physical protections. In addition, microscopic cell death observations and electrolyte leakage measurements indicated that RLs trigger a hypersensitive response-like defense in this plant. We also showed that foliar spray applications of RLs do not induce deleterious physiological consequences on plant growth or chlorophyll content and that RL protective properties are efficient on several grown cultivars of rapeseed. To our knowledge, this is the first report of RLs as an elicitor that suppresses fungal disease on tissues of an annual crop species under greenhouse conditions. Our results highlight the dual mode of action of these molecules exhibiting plant protection activation and antifungal activities and demonstrate their potential for crop cultures as environmental-friendly biocontrol solution.
Jacques F., Rippa S., Perrin Y. Physiology of L-carnitine in plants in light of the knowledge in animals and microorganisms. Plant Science, 2018, 9,1170. ABSTRACT: L-carnitine is present in all living kingdoms where it acts in diverse physiological processes. It is involved in lipid metabolism in animals and yeasts, notably as an essential cofactor of fatty acid intracellular trafficking. Its physiological significance is poorly understood in plants, but L-carnitine may be linked to fatty acid metabolism among other roles. Indeed, carnitine transferases activities and acylcarnitines are measured in plant tissues. Current knowledge of fatty acid trafficking in plants rules out acylcarnitines as intermediates of the peroxisomal and mitochondrial fatty acid metabolism, unlike in animals and yeasts. Instead, acylcarnitines could be involved in plastidial exportation of de novo fatty acid, or importation of fatty acids into the ER, for synthesis of specific glycerolipids. L-carnitine also contributes to cellular maintenance though antioxidant and osmolyte properties in animals and microbes. Recent data indicate similar features in plants, together with modulation of signaling pathways. The biosynthesis of L-carnitine in the plant cell shares similar precursors as in the animal and yeast cells. The elucidation of the biosynthesis pathway of L-carnitine, and the identification of the enzymes involved, is today essential to progress further in the comprehension of its biological significance in plants.
Ksouda G., Hajji M. Sellimi S., Merlier F., Falcimaigne Cordin A., Nasri M., Thomasset B. A Systematic Comparison of 25 Tunisian Plant Species Based on Oil and Phenolic Contents, Fatty Acid Composition and Antioxidant Activity. Ind. Crops Prod, 2018, 274,432-440. ABSTRACT: This study investigated 25 Tunisian plant species of 13 families based on their oil and total phenolic contents. The fatty acid profiles and antioxidant activity of phenolic extracts of these plant seeds were studied in order to identify species containing unusual fatty acids and potential antioxidants. The oil content varied from 4.2 to 66.5% of DW (Dry Weight). Fatty acid profiles were determined using gas chromatography analysis coupled to flame ionization detection and high resolution mass spectrometry (GC-FID and GC-HRMS). The results showed that plant seeds were mostly composed of classic fatty acids (palmitic, oleic, linoleic and linolenic acids), while unusual fatty acids such as long-chain and odd-chain fatty acids were minor components. However, the petroselinic acid was the major component of Apiaceae family seed oil, particularly in Pimpinella saxifraga and Pimpinella major. The phenolic content of seed methanolic extracts, measured by Folin
Merlier F., Imatoukene N., Octave S., Nicaud J. M., Thomasset B. A Gas Chromatography Full Scan High Resolution Orbitrap Mass Spectrometry Method for Separation and Characterization of 3-Hydroxymethyl Pyridine Ester of Fatty Acids at Low Levels. J. Chromatogr, 2018, 123,768-778. ABSTRACT: Fatty acid methyl esters (FAMEs), which are commonly used to characterize lipids, have several limitations to conclude on many structures. 3-Pyridylcarbinol esters (3-PCE) are used to characterize fatty acid structures , in particular, to identify ring and double bond positions on the carbon chain. Chromatographic separation of these esters is complex due to their polarity and high boiling points. In this study, we used a column with high resolutive power based on ionic liquids to increase the separation quality in gas chromatography (GC). In addition, we used a high-resolution detector (Orbitrap) to limit non-specific signals and improve the detection limits. This detector could be used with a mass filter at 5?ppm for the rapid determination of 3-PCE from its characteristic ions (m/z?=?108.0441 and 92.0495). This filter allowed the identification of derivative fatty acids with good sensibility. Thus, it was possible to characterize 3-PCE by measuring the exact fragment masses to confirm structures such as C19:2n12cyclo?9.
Socrier L., Quero A., Verdu M., Song Y., Milinie R., Mathiron D., Pilard S., Mesnard F., Monrandat S. Flax phenolic compounds as inhibitors of lipid oxidation: Elucidation of their mechanisms of action. Food Chemistry, 2018, 1575,72-79.
Husson E., Auxenfans T., Herbaut M., Baralle M., Lambertyn V., Rakotoarivonina H., Remond C. & Sarazin C. Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases.. Bioresource Technol., 2018, 251,280-287. ABSTRACT: Sequential and simultaneous strategies for fractioning wheat straw were developed in combining 1-ethyl-3-methyl imidazolium acetate [C2mim][OAc], endo-xylanases from Thermobacillus xylanilyticus and commercial cellulases. After [C2mim][OAc]-pretreatment, hydrolysis catalyzed by endo-xylanases of wheat straw led to efficient xylose production with very competitive yield (97.6?
Mehmood N., Alayoubi R., Husson E., Jacquard C., Buchs J., Sarazin C. & Gosselin I. Kluyveromyces marxianus, an attractive yeast for ethanolic fermentation in the presence of imidazolium ionic liquids.. Int. J. Molec. Sci., 2018, 19,887. ABSTRACT: Imidazolium ionic liquids (ILs) are promising solvents for lignocellulosic biomass (LCB) pretreatment and allow the achievement of higher ethanolic yields after enzymatic hydrolysis and ethanolic fermentation. However, residual ILs entrapped in pretreated biomass are often toxic for fermentative microorganisms, but interaction mechanisms between ILs and cells are still unknown. Here we studied the effects of 1-ethyl-3-methylimidazolium acetate [Emim][OAc] and 1-ethyl-3-methylimidazolium methylphosphonate [Emim][MeO(H)PO2] on Kluyveromyces marxianus, a thermotolerant ethanologenic yeast. Morphological impacts induced by ILs on K. marxianus were characterized by Scanning Electron Microscopy analysis and showed wrinkled, softened, and holed shapes. In Yeast-Malt-Dextrose (YMD) medium, K. marxianus tolerated IL additions up to 2% for [Emim][OAc] and 6% for [Emim][MeO(H)PO2]. Below these thresholds, some IL concentrations enhanced ethanolic yields up to +34% by switching the metabolic status from respiratory to fermentative. Finally, K. marxianus fermentation was applied on several substrates pretreated with [Emim][OAc] or [Emim][MeO(H)PO2] and enzymatically hydrolyzed: a model long fiber cellulose and two industrial LCBs, softwood (spruce) and hardwood (oak) sawdusts. The maximum ethanolic yields obtained were 1.8 to 3.9 times higher when substrates were pretreated with imidazolium ILs. Therefore K. marxianus is an interesting fermentative yeast in a second-generation bioethanol process implying IL pretreatment
Acket S., Degournay A., Gosset M., Merlier F., Troncoso-ponce M. A. & Thomasset B. Analysis of C-13 labeling amino acids by capillary electrophoresis - High resolution mass spectrometry in developing flaxseed.. Anal. Biochem., 2018, 547,14-18. ABSTRACT: n context of fluxomic studies, 13C labeling analysis of amino acids are very important for solving the carbon flow calculation, because they are synthesized in various biosynthesis pathways and cellular compartments in plant cells. Traditionally, 13C labeling analysis are performed using low resolution mass spectrometry detector by GC-MS. We compared a method using capillary electrophoresis-high resolution mass spectrometry without derivatization and with better accuracy assessment of labeling measurements comparing to classical GC-MS. Our method allowed us to show that valine, leucine, alanine are not synthesized from the same pyruvate pool during the period of reserves accumulation in flax seeds.
Tshabuse F., Farrant J. M., Humbert L., Moura D., Rainteau D., Espinasse C., Idrissi A., Merlier F., Acket S., Rafudeen M. S., Thomasset B. & Ruelland E. Glycerolipid analysis during desication and recovery of the resurrection plant Xerophyta humilis (Bak) Dur and Schinz.. Plant Cell Environ., 2018, 41,533-547. ABSTRACT: Feelings in humans are mental states representing groups of physiological functions that usually have defined behavioural purposes. Feelings, being evolutionarily ancient, are thought to be coordinated in the brain stem of animals. One function of the brain is to prioritise between competing mental states and, thus, groups of physiological functions and in turn behaviour. Plants use groups of coordinated physiological activities to deal with defined environmental situations but currently have no known mental state to prioritise any order of response. Plants do have a nervous system based on action potentials transmitted along phloem conduits but which in addition, through anastomoses and other cross?links, forms a complex network. The emergent potential for this excitable network to form a mental state is unknown, but it might be used to distinguish between different and even contradictory signals to the individual plant and thus determine a priority of response. This plant nervous system stretches throughout the whole plant providing the potential for assessment in all parts and commensurate with its self?organising, phenotypically plastic behaviour. Plasticity may, in turn, depend heavily on the instructive capabilities of local bioelectric fields enabling both a degree of behavioural independence but influenced by the condition of the whole plant.
Roblin G., Octave S., Faucher M., Fleurat-lessard P. & Berjeaud J. M. Cysteine: A multifaceted amino acid involved in signaling, plant resistance and antifungal development.. Plant Physiol. Biochem., 2018, 129,77-89. ABSTRACT: Early effects induced by cysteine were monitored using the model of Mimosa pudica pulvinar cells. Rapid dose-dependent membrane depolarization (within seconds) and modification of proton secretion (within minutes) were triggered at cysteine concentrations higher than 0.1?mM. These effects did not result from a modification of the plasma membrane H+-ATPase activity nor from a protonophore effect as shown by assays on plasma membrane vesicles isolated from pulvinar tissues. In a 0.5
Xu J., Prost E., Haupt K. & Tse Sum Bui B. Direct and sensitive determination of trypsin in human urine using a water-soluble signaling fluorescent molecularly imprinted polymer nanoprobe. . Sensor. Actuat. B-Chem. , 2018, 258,10-17. ABSTRACT: https://doi.org/10.1016/j.snb.2017.11.077
Mattsson L., Xu J., Preininger C., Tse Sum Bui B. & Haupt K. Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix.. Talanta, 2018, 181,190-196. ABSTRACT: We developed a competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines in fish samples. MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a complex sample matrix, such as fish extract, into the assay changes the environment and the binding conditions, therefore the importance of the sample preparation is extensively discussed. Several extraction and purification methods for fish were comprehensively studied, and an optimal clean-up procedure for fish samples using liquid-liquid extraction was developed. The feasibility of the competitive MIP assay was shown in the purified fish extract over a broad histamine range (1 ? 430ʵM). The MIP had the highest affinity towards histamine, but recognized also the structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine, providing simultaneous analysis and assessment of the total amount of biogenic amines.
Demir B., Lemberger M. M., Panagiotopoulou M., Medina Rangel P. X., Timur S., Hirsch T., Tse Sum Bui B., Wegener J. & Haupt K. Tracking hyaluronan: molecularly imprinted polymer coated carbon dots for cancer cell targeting and imaging.. ACS Appl. Mater. Interfaces, 2018, 10,3305-3313. ABSTRACT: War against cancer constantly requires new affinity tools to selectively detect, localize, and quantify biomarkers for diagnosis or prognosis. Herein, carbon nanodots (CDs), an emerging class of fluorescent nanomaterials, coupled with molecularly imprinted polymers (MIPs), are employed as a biocompatible optical imaging tool for probing cancer biomarkers. First, N-doped CDs were prepared by hydrothermal synthesis using starch as carbon source and l-tryptophan as nitrogen atom provider to achieve a high quantum yield of 25.1
Azouzi S., Santuz H., Morandat S., Pereira C., Cote F., Hermine O., El Kirat K., Colin Y., Le Van Kim C., Etchebest C. & Amireault P. Antioxidant and membrane binding properties of serotonin protect lipids from oxidation.. Biophys. J., 2017, 112,1863-1873. ABSTRACT: Serotonin (5-hydroxytryptamine, 5-HT) is a well-known neurotransmitter that is involved in a growing number of functions in peripheral tissues. Recent studies have shown nonpharmacological functions of 5-HT linked to its chemical properties. Indeed, it was reported that 5-HT may, on the one hand, bind lipid membranes and, on the other hand, protect red blood cells through a mechanism independent of its specific receptors. To better understand these underevaluated properties of 5-HT, we combined biochemical, biophysical, and molecular dynamics simulations approaches to characterize, at the molecular level, the antioxidant capacity of 5-HT and its interaction with lipid membranes. To do so, 5-HT was added to red blood cells and lipid membranes bearing different degrees of unsaturation. Our results demonstrate that 5-HT acts as a potent antioxidant and binds with a superior affinity to lipids with unsaturation on both alkyl chains. We show that 5-HT locates at the hydrophobic-hydrophilic interface, below the glycerol group. This interfacial location is stabilized by hydrogen bonds between the 5-HT hydroxyl group and lipid headgroups and allows 5-HT to intercept reactive oxygen species, preventing membrane oxidation. Experimental and molecular dynamics simulations using membrane enriched with oxidized lipids converge to further reveal that 5-HT contributes to the termination of lipid peroxidation by direct interaction with active groups of these lipids and could also contribute to limit the production of new radicals. Taken together, our results identify 5-HT as a potent inhibitor of lipid peroxidation and offer a different perspective on the role of this pleiotropic molecule.
Xu J, Haupt K. & Tse Sum Bui B. Core-shell molecularly imprinted polymer nanoparticles as synthetic antibodies in a sandwich fluoroimmunoassay for trypsin determination in human serum.. ACS Appl. Mater. Interfaces, 2017, 9, 24476?24483. ABSTRACT: We describe the application of a fluorescently labeled water-soluble core
Yarman A., Jetzschmann K. J., Neumann B., Zhang X., Wollenberger U., Cordin A., Haupt K. & Scheller F. W. Enzymes as tools in MIP-sensors.. Chemosensors, 2017, 5,11. ABSTRACT: Molecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and are potentially cheaper to produce. However, the affinity and especially the selectivity of MIPs are in general lower than those of their biological pendants. Enzymes are useful tools for the preparation of MIPs for both low and high-molecular weight targets: As a green alternative to the well-established methods of chemical polymerization, enzyme-initiated polymerization has been introduced and the removal of protein templates by proteases has been successfully applied. Furthermore, MIPs have been coupled with enzymes in order to enhance the analytical performance of biomimetic sensors: Enzymes have been used in MIP-sensors as
Antonitti, V., Boudesoc, S., Dupont, L., Farvacques, N., Cezarda, C., Da Nascimento, S., Raimbert, J-f., Socrier, L., Robin, T-j., Morandat, S., El-kirat, K., Mullie, C., Sonnet, P. Synthesis, iron(III) complexation properties, molecular dynamics simulations and P. aeruginosa siderophore-like activity of two pyoverdine analogs. European Journal of Medicinal Chemistry, 2017, 137,338-350. ABSTRACT: P. aeruginosa ranks among the top five organisms causing nosocomial infections. Among the many novel strategies for developing new therapeutics against infection, targeting iron uptake mechanism seems promising as P.
Socrier L., Rosselin M., Durand G. & Morandat S. Cholesterol-nitrone conjugates as protective agents against lipid oxidation: A model membrane study. BBA-Biomembranes, 2017, 1859,2495-2504. ABSTRACT: Free radical scavengers such as ?-phenyl-N-tert-butylnitrone (PBN) have been widely used as protective agents in several biological models. We recently designed two PBN derivatives by adding a cholesterol moiety to the parent nitrone to increase its lipophilicity. In addition to the cholesterol, a sugar group was also grafted to enhance the hydrophilic properties at the same time. In the present work we report on the synthesis of a third derivative bearing only a cholesterol moiety and the physical chemical and antioxidant characterization of these three derivatives. We demonstrated they were able to form stable monolayers at the air/water interface and with the two derivatives bearing a sugar group, repulsive interactions with 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (DLPC) were observed. We next investigated the interaction with DLPC on a liposome model. Fluorescence spectroscopy experiments showed the addition of a cholesterol moiety causes an ordering effect whereas the presence of the sugar group led to a disordering effect. The protective effect against lipid oxidation was then investigated using dynamic light scattering and the formation of conjugated dienes was quantified spectrophotometrically. Two oxidizing systems were tested, i.e. the AAPH-thermolysis which generates peroxyl radicals and the Fenton reagent which is responsible of the formation of hydroxyl radicals. Due to their membrane localization, the three cholesteryl-PBN derivatives are able to prevent lipid oxidation with the two types of radical inducers but with a different mode of action.
Mourao C. A., Bokeloh F., Xu J., Prost E., Duma L., Merlier F., Bueno S. M. A., Haupt K. & Tse Sum Bui, B. Dual oriented solid phase molecular imprinting: Toward selective artificial receptors for recognition of nucleotides in water. Macromolecules, 2017, 50,7484-7490. ABSTRACT: We describe the synthesis of water-soluble molecularly imprinted polymer nanoparticles (MIP-NPs) as a new artificial host receptor for the recognition of adenosine monophosphate (AMP), used herein, as a model nucleotide. MIP-NPs were prepared by solid-phase synthesis on glass beads (GB) using, for the first time, immobilized Fe(III)-chelate as an affinity ligand to orientate the AMP via its phosphate group. A polymerizable thymine monomer which can induce complementary base-pairing with the adenine moiety of the nucleotide was synthesized and incorporated in the polymerization mixture to constrain the AMP in a dual-orientated configuration. The MIP-NPs were remarkably selective toward AMP as they did not bind other nucleotides, GMP, UMP, and CMP. This strategy of using the phosphate group of AMP as a hinge enables unhindered pairing of the nucleobase with its corresponding complementary base monomer and can be extended to the preparation of specific MIP receptors for other key nucleotides in aqueous conditions.
Merlier F., Jellali R., Leclerc E. Online monitoring of hepatic rat metabolism by coupling a liver biochip and a mass spectrometer. Analyst, 2017, 142,3747-3757. ABSTRACT: A microfluidic liver biochip was coupled with a mass spectrometer to detect in real time the drug metabolism of hepatocytes. The hepatocytes were cultivated in the biochip for 35 h. The biochip was placed in a small-scale incubator in which the temperature and CO2 concentration were controlled. The biochip was connected serially to a mass spectrometer, a peristaltic pump and a culture medium reservoir. The injection in the mass spectrometer was performed every 10 min for 11 h. The metabolism of midazolam, phenacetin, omeprazole, dextromethorphan, repaglinide, rosuvastatin, tolbutamide and caffeine was investigated. We monitored the apparition of omeprazole sulfone, hydroxy omeprazole, repaglinide glucuronide, rosuvastatin lactone, dextrorphan, 1-hydroxy midazolam, 4-hydroxy midazolam, 1,4-hydroxy midazolam, paracetamol and 1,3-methylxanthine. Although these were observed, hydroxytolbutamide, 3-methoxymorphinan and midazolam glucuronide, hydroxy repaglinide were not detected. Based on a pharmacokinetic model, we calculated in vitro intrinsic clearances in which adsorption onto the perfusion circuit was taken into account. Then, using a liver organ model, we extrapolated the in vitro intrinsic clearances to the in vivo clearances. The estimated in vivo clearances were in agreement with the literature data on rats for midazolam, dextromethorphan, phenacetin, tolbutamide and caffeine. Rosuvastatin, omeprazole and repaglinide prediction underestimated the in vivo data.
Veneziano R., Rossi C., Chenal A., Brenner C., Ladant D., Chopineau J. Synthesis and characterization of tethered lipid assemblies for membrane protein reconstitution. Biointerphases, 2017, 12,04E301. ABSTRACT: Biological membranes and their related molecular mechanisms are essential for all living organisms. Membranes host numerous proteins and are responsible for the exchange of molecules and ions, cell signaling, and cell compartmentation. Indeed, the plasma membrane delimits the intracellular compartment from the extracellular environment and intracellular membranes. Biological membranes also play a major role in metabolism regulation and cellular physiology (e.g., mitochondrial membranes). The elaboration of membrane based biomimetic systems allows us to reconstitute and investigate, in controlled conditions, biological events occurring at the membrane interface. A whole variety of model membrane systems have been developed in the last few decades. Among these models, supported membranes were developed on various hydrophilic supports. The use of solid supports enables the direct use of surface sensitive techniques (e.g., surface plasmon resonance, quartz crystal microbalance, and atomic force microscopy) to monitor and quantify events occurring at the membrane surface. Tethered bilayer membranes (tBLMs) could be considered as an achievement of the first solid supported membranes described by the McConnell group. Tethered bilayers on solid supports were designed to delimit an inside compartment from an outside one. They were used for measuring interactions with ligands or incorporating large membrane proteins or complexes without interference with the support. In this context, the authors developed an easy concept of versatile tBLMs assembled on amino coated substrates that are formed upon the vesicle fusion rupture process applicable to protein-free vesicles as well as proteoliposomes. The phospholipid bilayer (natural or synthetic lipids) incorporated 5% of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-poly ethylene glycol-N-hydroxy succinimide to ensure the anchorage of the bilayer to the amino coated surface. The conditions for the formation of tBLMs on amino-coated gold and glass were optimized for protein-free vesicles. This biomimetic membrane delimits an inside
Brahim M., Checa Fernandez B. L., Regnier O., Bousseta N., Grimi N., Sarazin C., Husson E., Vorobiev E., Brosse N. Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straws lignin and pulps. Bioresource Technology, 2017, 237,42308
Husson E., Hadad C., Huet G., Laclef S., Lesur D., Lambertyn V., Jamali A., Gottis S., Sarazin C., Nguyen Van Nhien A. The effect of room temperature ionic liquids on the selective biocatalytic hydrolysis of chitin via sequential or simultaneous strategies. Greeen Chemistry, 2017, 19,4122-4131. ABSTRACT: An efficient conversion of chitin, the second most abundant renewable polymer on the Earth, into N-acetylglucosamine and N,N?-diacetylchitobiose, using room temperature ionic liquids (RTILs) and commercially available chitinases is described for the first time. The sequential strategy consists of the use of RTILs to pretreat chitin under mild conditions as a first step before enzymatic hydrolysis. [C2mim][OAc] (1-ethyl-3-methyl imidazolium) pretreatment provides an efficient production of N-acetylglucosamine (185.0
Nail Nasir M., Lins L., Crowet J. M., Ongena M., Dorey S., Dhondt-cordelier S., Clement C., Bouquillon S., Haudrechy A., Sarazin C., Fauconnier M. L., Nottk., Deleu M. Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straws lignin and pulps. Langmuir, 2017, 33,9979-9987. ABSTRACT: Natural and synthetic amphiphilic molecules including lipopeptides, lipopolysaccharides, and glycolipids are able to induce defense mechanisms in plants. In the present work, the perception of two synthetic C14 rhamnolipids, namely, Alk-RL and Ac-RL, differing only at the level of the lipid tail terminal group have been investigated using biological and biophysical approaches. We showed that Alk-RL induces a stronger early signaling response in tobacco cell suspensions than does Ac-RL. The interactions of both synthetic RLs with simplified biomimetic membranes were further analyzed using experimental and in silico approaches. Our results indicate that the interactions of Alk-RL and Ac-RL with lipids were different in terms of insertion and molecular responses and were dependent on the lipid composition of model membranes. A more favorable insertion of Alk-RL than Ac-RL into lipid membranes is observed. Alk-RL forms more stable molecular assemblies than Ac-RL with phospholipids and sterols. At the molecular level, the presence of sterols tends to increase the RLs
Garcia-soto M., Haupt K., Gonzato C. Synthesis of molecularly imprinted polymers by photo-iniferter polymerization under visible light.. Polym. Chem., 2017, 8,4830-4834. ABSTRACT: The easiest way to make polymers able to specifically recognize a target molecule consists of copolymerizing functional monomers and a cross-linker around a molecular template. This method affords a rigid material bearing high affinity binding sites, namely a synthetic receptor or antibody mimic, known as the
Acket S., Degournay A., Merlier F. & Thomasset B. Data documenting the comparison between the theoretically expected values of free sugars mass isotopomer composition with standards using GC-MS and LC-HRMS for Metabolic Flux Analysis.. Data in Brief, 2017, 12,108-112. ABSTRACT: The data presented in this article are related to the research article entitled
Hodrogue A., Trecherel E., Cornu M., Darwiche W., Mansour A., Ait-mohand K., Verissimo T., Gomila C., Schembri C., Da Nascimento S., Elboutachfait R., Boulier A., Lorne E., Courtois J., Petit E., Toumieux S., Kovensky J., Sonnet P., Massy Z. A., Kamel S. Rossi C., Ausseil J. Oligogalacturonic acid inhibits vascular calcification by two mechanisms: inhibition of VSMC osteogenic conversion and interaction with collagen. Arteriosclerosis, Thrombosis, and Vascular Biology, 2017, 37,1391-1401.
Maes T., Jessop R., Wellner N., Haupt K. & Mayes A. G. A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red. Sci. Rep., 2017, 7,44501. ABSTRACT: A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.
Attieh M. D., Zhao Y., Elkak A., Falcimaigne-cordin A., & Haupt K. Enzyme-initiated free-radical polymerization of molecularly imprinted polymer nanogels on a solid phase with an immobilized radical source. Angew. Chem. Int. Ed., 2017, 56,3339-3343. ABSTRACT: An enzyme?mediated synthetic approach is described for the preparation of molecularly imprinted polymer nanoparticles (MIP?NPs) in aqueous media. Horseradish peroxidase (HRP) was used to initiate the polymerization of methacrylate or vinyl monomers and cross?linkers by catalyzing the generation of free radicals. To prevent entrapment of the enzyme in the cross?linked polymer, and to enable it to be reused, HRP was immobilized on a solid support. MIPs based on 4?vinylpyridine and 1,4?bis(acryloyl)piperazine for the recognition of 2,4?dichlorophenoxyacetic acid (2,4?D) and salicylic acid were synthesized in an aqueous medium. MIPs for the protein trypsin were also synthesized. MIP nanoparticles with sizes between 50 and 300?nm were obtained with good binding properties, a good imprinting effect, and high selectivity for the target molecule. The reusability of immobilized HRP for MIP synthesis was shown for several batches.
Karczmarczyk, A., Haupt K. & Feller K. H. Development of a QCM-D biosensor for Ochratoxin A detection in red wine. Talanta, 2017, 166,193-197. ABSTRACT: Ochratoxin A (OTA), a highly toxic compound, is one of the most widely spread mycotoxins that contaminates a large variety of agricultural commodities. Due to its presence in the food chain, it imposes a hazard on both human and animal health. Therefore, there is a need for precise, fast and simple methods for toxin quantification. Herein, a novel sensor based on a quartz crystal microbalance with dissipation monitoring (QCM-D) and antibodies for specific analyte recognition was developed for rapid and sensitive detection of OTA in red wine. The combination of indirect competitive assay with QCM-D gives a straightforward device, which can simultaneously measure frequency (?f) and dissipation (?D) changes resulting in detailed information about the mass attached to the sensor surface as well as conformational changes, viscoelastic properties and the hydration state of the film. Small molecules (such as OTA) suffer from poor LOD due to the high concentration of primary antibody needed to generate adequate signal. In the present study, amplification of the QCM-D signal was obtained by applying secondary antibodies conjugated with gold nanoparticles (AuNPs). Thanks to this, a linear detection range of 0.2
Brahim M., Ferandez B. L. C., Regnier O., Boussetta N., Grimi N., Sarazin C., Husson E., Vorobiev E., Brosse N Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straw's lignin and pulps. Bioressource Technology , 2017, 237,11-19. ABSTRACT: In this study, ultrasound (US) and high voltage electrical discharges (HVED) were combined with chemical treatments (soda or organosolv) for rapeseed straw delignification. Delignification was improved by both physical pretreatments. US increased the extractability of hemicelluloses and HVED induced a partial degradation of cellulose. Best synergies were observed for HVED-soda and US-organosolv treatments. The obtained lignin fractions were characterized with 13C NMR and 2D 1H
Imatoukenen N., Verbek J., Beopoulos A., Idrissi Taghki A., Thomasset B., Sarde C. O., Nonus M. & Nicaud J. M. Metabolic engineering for Conjugated Linoleic Acid production in the oleaginous yeast in Yarrowia lipolytica.. Applied Microbiol. Biotechnol, 2017, 101,4605-4616. ABSTRACT: Conjugated linoleic acids (CLAs) have been found to have beneficial effects on human health when used as dietary supplements. However, their availability is limited because pure, chemistry-based production is expensive, and biology-based fermentation methods can only create small quantities. In an effort to enhance microbial production of CLAs, four genetically modified strains of the oleaginous yeast Yarrowia lipolytica were generated. These mutants presented various genetic modifications, including the elimination of ?-oxidation (pox1-6
Rombaut N., Savoire R., Van Hecke E. Et Thomasset B. Supercritical CO2 extraction of linseed: Optimization by experimental design with regards to oil yield and composition. Eur. J. Lipid Sci. Technol, 2017, 119,1600078. ABSTRACT: The aim of the present study is to evaluate the effect of CO2 pressure, temperature, and CO2 flow rate on supercritical fluid extraction of linseed oil using neat CO2. Chosen methodology is based on central composite rotatable design, responses studied are extract yield, oil yield, water?extract ratio, oil ??tocopherol content, oil polyphenols content, alpha linolenic acid proportion in oil, and oil acid value. Acid value, tocopherol content, and linolenic acid proportion are independent responses of studied factors. On other responses, only pressure and temperature have an influence. Based on response surface analysis and multiresponses optimization, optimal conditions for oil yield, and oil polyphenol content maximization and water?extract minimization were determined (42.5?MPa, 120
Hano C., Corbin C., Quero A., Rombaut N., Savoire R., Molinie R., Thomasset B., Mesnard F. & Laine E. The use of (+)-secoisolariciresinol extracted from the hulles as en effective protectant of linseed oil and its emulsion against oxidative damage. . Eur. J. Lipid Sci. Technol, 2017, 118,
Aimee Rasolohery C., Ermenegilde Ralaiba B., Ayerdi Gotor A., Merlier F., Benja R., Rakotovao M., Rhazi L. Chemical Characterization and Antioxidant Potential of Athroisma protei- formis Essential Oil. The Natural Products Journal, 2017, 7,208-215.
Hano C., Corbin C., Drouet S., Quero A., Rombaut N., Savoire R., MoliniÉ R., Thomasset B., Mesnard F., LainÉ E. The lignan (+)-secoisolariciresinol extracted from flax hulls is an effective protectant of linseed oil and its emulsion against oxidative damage . Eur. J. Lipid Sci. Technol. , 2017, 119,1600219. ABSTRACT: Secoisolariciresinol (SECO) is a natural antioxidant lignan accumulated in large amounts in the seedcoat of flax and retained in the flaxseed cake residue during linseed oil pressing. Here SECO was extracted and purified from flaxseed cake and assayed for its ability to prevent oxidation of linseed oil and an o/w emulsion containing linseed oil. For this purpose, an accelerated storage (Schaal oven) test was performed and SECO effectiveness was compared to that of two antioxidants commonly used in food and cosmetic products: ??tocopherol (??TOCO) and butylated hydroxyanisole (BHA). In our hands, SECO addition, ranging from 50 to 500??mole per kg oil, significantly decreased the production of both primary (conjugated dienes, CD) and secondary (thiobarbituric acid?reactive substances, TBARS) oxidation products. This study evidenced that SECO is an effective stabilizer of linseed oil and its o/w emulsion and this protective effect outperformed both the natural ??TOCO and the synthetic BHA antioxidants. In particular, SECO was the most effective in the protection of the o/w emulsion against secondary oxidation products, which makes it a potential alternative preservative for oily products in foods and cosmetics.
Auxenfans T., Husson E. & Sarazin C. Simultaneous pretreatment and enzymatic saccharification of (ligno)celluloses in aqueous-ionic liquid media: a compromise.. Biochem. Eng. J., 2017, 117,77-86. ABSTRACT: In view of decreasing the amount of IL to achieve efficient simultaneous pretreatment and saccharification, a comprehensive study was undertaken. Different types of lignocellulosic biomasses were investigated in various enzymatic aqueous-IL systems including 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) or 1-ethyl-3-methylimidazolium methylphosphonate ([C2mim][MeO(H)PO2]). To better understand how 10% (v/v) of IL in the reaction medium led to the highest yields of glucose without fractionation from sawdust, distinct cellulosic models were then used as substrates. Kinetic studies demonstrated that both ILs affect synergistic action of cellulolytic enzymes depending on both constitutive anion and cellulosic substrate. Concentrations above 10% v/v of ILs deactivated cellulase even on highly digestible model substrates. ? and ? Kamlet-Taft parameters constituted more physicochemical pertinent indicators than apparent pH value to investigate effects of IL on cellulase performances. Fine description of these effects was proposed onto individual EG, CBH and BG. [C2mim][MeO(H)PO2] was demonstrated a better compatible IL for enzymes up to a concentration of 30% (v/v). The efficiency of simultaneous pretreatment and saccharification was governed by a compromise between better substrate accessibility and enzyme deactivation.
Acket S., Degournay A., Merlier F. & Thomasset B. 13C labeling analysis of sugars by hight resolution-mass spectrometry for metabolic flux analysis.. Anal. Biochem., 2017, 527,45-48. ABSTRACT: Metabolic flux analysis is particularly complex in plant cells because of highly compartmented metabolism. Analysis of free sugars is interesting because it provides data to define fluxes around hexose, pentose, and triose phosphate pools in different compartment. In this work, we present a method to analyze the isotopomer distribution of free sugars labeled with carbon 13 using a liquid chromatography
Shahsavarian M., Chaaya N., Costa N., Boquet D., Atkinson A., Offmann B., Kaveri Sv., Lacroix-desmazes S., Friboulet A., Avalle B. & Padiolleau-lefevre S. Multi-target selection of catalytic antibodies wih . FEBS J., 2017, 284,634-653. ABSTRACT: ??lactamase enzymes responsible for bacterial resistance to antibiotics are among the most important health threats to the human population today. Understanding the increasingly vast structural motifs responsible for the catalytic mechanism of ??lactamases will help improve the future design of new generation antibiotics and mechanism?based inhibitors of these enzymes. Here we report the construction of a large murine single chain fragment variable (scFv) phage display library of size 2.7 ? 109 with extended diversity by combining different mouse models. We have used two molecularly different inhibitors of the R?TEM ??lactamase as targets for selection of catalytic antibodies with ??lactamase activity. This novel methodology has led to the isolation of five antibody fragments, which are all capable of hydrolyzing the ??lactam ring. Structural modeling of the selected scFv has revealed the presence of different motifs in each of the antibody fragments potentially responsible for their catalytic activity. Our results confirm (a) the validity of using our two target inhibitors for the in vitro selection of catalytic antibodies endowed with ??lactamase activity, and (b) the plasticity of the ??lactamase active site responsible for the wide resistance of these enzymes to clinically available inhibitors and antibiotics.
Panagiotopoulou M., Kunath S., Medina-rangel P. X., Haupt K. & Tse Sum Bui B. Fluorescent molecularly imprinted polymers as plastic antibodies for selective labeling and imaging of hyaluronan and sialic acid on fixed and living cells.. Biosens. Bioelectron., 2017, 88,85-93. ABSTRACT: Altered glycosylation levels or distribution of sialic acids (SA) or hyaluronan in animal cells are indicators of pathological conditions like infection or malignancy. We applied fluorescently-labeled molecularly imprinted polymer (MIP) particles for bioimaging of fixed and living human keratinocytes, to localize hyaluronan and sialylation sites. MIPs were prepared with the templates D-glucuronic acid (GlcA), a substructure of hyaluronan, and N-acetylneuraminic acid (NANA), the most common member of SA. Both MIPs were found to be highly selective towards their target monosaccharides, as no cross-reactivity was observed with other sugars like N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, D-glucose and D-galactose, present on the cell surface. The dye rhodamine and two InP/ZnS quantum dots (QDs) emitting in the green and in the red regions were used as fluorescent probes. Rhodamine-MIPGlcA and rhodamine-MIPNANA were synthesized as monodispersed 400 nm sized particles and were found to bind selectively their targets located in the extracellular region, as imaged by epifluorescence and confocal microscopy. In contrast, when MIP-GlcA and MIP-NANA particles with a smaller size (125 nm) were used, the MIPs being synthesized as thin shells around green and red emitting QDs respectively, it was possible to stain the intracellular and pericellular regions as well. In addition, simultaneous dual-color imaging with the two different colored QDs-MIPs was demonstrated. Importantly, the MIPs were not cytotoxic and did not affect cell viability; neither was the cells morphology affected as demonstrated by live cell imaging. These synthetic receptors could offer a new and promising imaging tool to monitor disease progression.
Ramsay A., Fliniaux O., Quero A., Molinie R., Demailly H., Hano C., Paetz C., Roscher A., Grand E., Kovensky J., Schneider B. & Mesnard F. Kinetics of the incorporation of the main phenolic compounds into the lignan macromolecule during flaxseed development.. Food Chem., 2017, 217,1-8.
Quero A., Molinie R., Mathiron D., Thiombiano B., Fontaine J. F., Brancourt D., Van Wuytswinkel O., Petit E., Demailly H., Mongelard G., Pilard S., Thomasset B. & Mesnard F. Metabolite profiling in developing Camelina sativa seeds.. Metabolomics, 2016, 12,186-199.
Nestora S., Merlier F., Prost E., Haupt K., Rossi C. & Tse Sum Bui B. Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer.. J. Chromatogr. A, 2016, 1465,47-54.
Canfarotta F., Waters A., Sadler R., Mcgill P., Guerreiro A., Papkovsky D., Haupt K. & Piletsky S. Biocompatibility and internalization of moleculary imprinted nanoparticles.. Nano Res., 2016, 9,3463-3477.
Chia Gomez L. P., Spangenberg A., Ton X. A., Fuchs Y., Bokeloh F., Malval J. P., Tse Sum Bui B., Thuau D., Ayela C., Haupt K. & Soppera O. Rapid prototyping of chemical microsensors based on molecularly imprinted polymers synthesized by two-photon stereolithography.. Adv. Mater., 2016, 28,5931-5937.
Panagiotopoulou M., Salinas Y., Beyazit S., Kunath S., Mayes A. G., Duma L., Prost E., Resmini M., Tse Sum Bui B. & Haupt K. Molecularly imprinted polymer-coated quantum dots for multiplexed cell targeting and imaging.. Angew. Chem. Int. Ed., 2016, 55,8244-8248.
Nestora S., Merlier F., Beyazit S., Prost E., Duma L., Baril B., Graves A., Haupt K. & Tse Sum Bui B. Plastic antibodies for cosmetics: Molecularly imprinted polymers scavenge precursors of malodors. . Angew. Chem. Int. Ed., 2016, 55,6252-6256.
Tanvir S., Thuroczy G., Selmaoui B., Pires Antonietti V. S., Sonnet P., Arnaud-cormos D., Leveque P., Pulvin S. & De Seze R. Effects of 3G cell phone exposure on the structure and function of the human cytochrome P450 reductase.. Bioelectrochem., 2016, 111,62-69.
Xu J. J., Ambrosini S., Tamahkar E., Rossi C., Haupt K. & Tse Sum Bui B. Toward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins.. Biomacromolecules, 2016, 17,345-353.
Jellali R., Bricks T., Jacques S., Fleury M. J., Paullier P., Merlier F. & Leclerc E. Long term human primary hepatocyte cultures in a microfluidic liver biochip show maintenance of mRNA levels and higher drugs metabolisms when compared to Petri cultures.. Biopharm. Drug Disp., 2016, 37,264-275.
Bonomi P., Attieh M. D., Gonzato C. & Haupt K. A novel versatile water soluble iniferter platform for the preparation of imprinted nanoparticles via photopolymerization in aqueous media.. Chem.-Eur. J., 2016, 22,10150-10154.
Mahendra A., Peyron I., Thaunat O., Dollinger C., Gilardin L., Sharma M., Wootla B., Rao D. N., Padiolleau-lefevre S., Boquet D., More A., Varadarajan N., Kaveri S. V., Legendre C. & Lacroix-desmazes S. Generation of catalytic antibodies is an intrinsic property of an individual's immune system: a study on a large cohort of renal transplant patients.. J. Immunol., 2016, 196,4075-4081.
Saha C., Das M., Stephen-victor E., Friboulet A., Bayry J. & Kaveri S. Differential effect of Viscum album preparations on the maturation and activation of human dendritic cells and CD4+ T cell responses.. Molecules, 2016, 21,912.
Kunath S., Panagiotopoulou M., Maximilien J., Marchyk N., Sanger J. & Haupt K. Cell and tissue imaging with molecularly imprinted polymers as plastic antibody mimics.. Adv. Healthc. Mater., 2015, 4,1322-1326.
Adali-kaya Z., Tse Sum Bui B., Falcimaigne-cordin A. & Haupt K. Molecularly imprinted polymer nanomaterials and nanocomposites: atom-transfer radical polymerization with acidic monomers.. Angew. Chem. Int. Ed., 2015, 54,192-195.
Bricks T., Hamon J., Fleury M. J., Jellali R., Merlier F., Herpe Y. E., Seyer A., Regimbeau J. M., Bois F. & Leclerc E. Investigation of omeprazole and phenacetin frist-pass metabolism in humans using a microscale bioreactor and pharmacokinetic models.. Biopharm. Drug Disp., 2015, 36,275-293.
Ton X. A., Acha V., Bonomi P., Tse Sum Bui B. & Haupt K. A disposable evanescent wave fiber optic sensor coated with a molecularly imprinted polymer as a selective fluorescent probe.. Biosens. Bioelectron., 2015, 64,359-366.
Mehmood N., Husson E., Jacquard C., Wewetzer S., Buchs J., Sarazin C. & Gosselin I. Impact of two ionic liquids, 1-ethyl-3-methylimidazolium acetate and 1-ethyl-3-methylimidazolium methylphosphonate, on Saccharomyces cerevisiae: metabolic, physiologic, and morphological investigations.. Biotechnol. Biofuels , 2015, 8,n.17.
Fabre G., Bayach I., Berka K., Paloncyova M., Starok M., Rossi C., Duroux J. L., Otyepka M. & Trouillas P. Synergism of antioxidant action of vitamins E and Quercetin is related to formation of molecular associations in biomembranes.. Chem. Commun., 2015, 51,7713-7716.
Lazouk M. A., Savoire R., Kaddour A., Castello J., Lanoiselle J. L., Van Hecke E. & Thomasset B. Oilseeds sorption isoterms, mechanical properties and pressing.: Global view of water impact.. J. Food Eng., 2015, 153,73-80.
Savoire R., Lazouk M. A., Van-hecke E., Roulard R., Tavernier R., Guillot X., Rhazi L., Petit E., Mesnard F. & Thomasset B. Environmental and varietal impact on linseed composition and on oil unidirectional expression process.. OCL, 2015, 22,D605.
Nowacki L., Vigneron P., Rotellini L., Cazzola H., Merlier F., Prost E., Ralanairina R., Gadonna J. P., Rossi C. & Vayssade M. Betanin-enriched red beetroot (Beta vulgaris L.) extract induces apoptosis and autophagic cell death in MCF-7 cells.. Phytother. Res., 2015, 29,1964-1973.
Panagiotopoulou M., Beyazit S., Nestora S., Haupt K., & Tse Sum Bui B. Initiator-free synthesis of molecularly imprinted polymers by polymerization of self-initiated monomers.. Polymer, 2015, 66,43-51.
Foguel M. V., Ton X. A., Zanoni M. V. B., Sotomayor M. D. P. T., Haupt K. & Tse Sum Bui B. A molecularly imprinted polymer-based evanescent wave fiber optic sensor for the detection of basic red 9 dye. . Sens. Actuators B, 2015, 218,222-228.
Elluru S. R., Saha C., Hedge P., Friboulet A., Bayry J. & Kaveri S. Dissecting the anti-inflammatory effects of Viscum album: inhibition of cytokine-induced expression of cyclooxygenase-2 and secretion of prostaglandin E2.. Translat. Res. Biomed., 2015, 4,67-73.
Ponomarenko N., Chatziefthimiou S. D., Kurkova I., Mokrushina Y., Stepanova A., Smirnov I., Avakyan M., Bobik T., Mamedov A., Mitkevich V., Belogurov A., Fedorova O., Dubina M., Golovin A., Lamzin V., Friboulet A., Makarov A., Wilmanns M. & Gabibov A. Role of . Acta Crystallogr. D, 2014, 70,708-719.
Boultadakis-arapinis M., Gandon V., Prost E., Micouin L. & Lecourt T. Electronic effects in carbene-mediated C-H bond functionalization: an experimental and theoretical study.. Adv. Synth. Catal., 2014, 356,2493-2505.
Beyazit S., Ambrosini S., Marchyk N., Palo E., Kale V., Soukka T., Tse Sum Bui B. & Haupt K. Versatile synthetic strategy for coating upconverting nanoparticles with polymer shells through localized photopolymerization by using the particles as internal light sources.. Angew. Chem. Int. Ed., 2014, 53,8919-8923.
Auxenfans T., Buchoux S., Husson E. & Sarazin C. Efficient enzymatic saccharification of Miscanthus: energy-saving by combinig dilute acid and ionic liquid pretreatments.. Biomass Bioenerg., 2014, 62,82-92.
Prot J. M., Maciel L., Bricks T., Merlier F., Cotton J., Paullier P., Bois F. & Leclerc E. First pass intestinal and liver metabolism of paracetamol in a microfluidic platform coupled with a mathematical modeling as a means of evaluating ADME processes in humans.. Biotechnol. Bioeng., 2014, 111,2027-2040.
Rombaut N., Savoire R., Thomasset B., Belliard T., Castello J., Van Hecke E. & Lanoiselle J. L. Grape seed oil extraction: interest of supercritical fluid extraction and gas assisted mechanical extraction for enhancing polyphenol co-extraction in oil.. C.R. Chimie, 2014, 17,284-292.
Ortial S., Morandat S., Bortolato M., Roux B., Polidori A., Pucci B. & Durand G. PBN derived amphiphilic spin-traps. II/ Study of their antioxidant properties in biomimetic membranes.. Colloids Surf. B, 2014, 113,384-393.
Auxenfans T., Buchoux S., Larcher T., Husson G., Husson E. & Sarazin C. Enzymatic saccharification and structural properties of industrial wood sawdust: recycled ionic liquids pretreatments.. Energ. Convers. Manage., 2014, 88,1094-1103.
Gervaise C., Bonnet V., Nolay F., Cezard C., Stasik I., Sarazin C. & Djedaini-pilard F. Diesterification of 3-[(ß-cyclodextrinyl)succinamido]propane-1,2-diol catalyzed by lipase: Diastereoselectivity or tridimensional substrate specificity?. Eur. J. Org. Chem., 2014, 2014,6200-6209.
Shahsavarian M., Le Minoux D., Matti K. M., Kaveri S., Lacroix-desmazes S., Boquet D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Exploitation of rolling circle amplification for the construction of large phafe display antibody libraries.. J. Immunol. Methods, 2014, 407,26-34.
Li B., Xu J., Hall A. J., Haupt K. & Tse Sum Bui B. Water-compatible silica sol-gel molecularly imprinted polymer as potential delivery system for the controlled release of salicylic acid.. J. Mol. Recognit., 2014, 27,559-565.
Ramsay A., Fliniaux O., Fang J., Molinie R., Roscher A., Grand E., Guillot X., Kovensky J., Fliniaux M. A., Schneider B. & Mesnard F. Development of an NMR metabolomics-based tool for selection of flaxseed varieties.. Metabolomics, 2014, 10,1258-1267.
Fliniaux O., Corbin C., Ramsay A., Renouard S., Beejmohun V., Doussot J., Falguieres A., Ferroud C., Lamblin F., Laine E., Roscher A., Grand E., Mesnard F. & Hano C. Microwave-assisted extraction of herbacetin diglucoside from flax (Linum usitatissimum L.) seed cakes and its quantification using RP-HPLC-UV system.. Molecules, 2014, 19,3025-3037.
Marchyk N., Maximilien J., Beyazit S., Haupt K. & Tse Sum Bui B. One-pot synthesis of iniferter-bound polystyrene core nanoparticles for the controlled grafting of multilayer shells.. Nanoscale, 2014, 6,2872-2878.
Gonzato C., Pasetto P., Bedoui F., Mazeran P. E. & Haupt K. On the effect of using RAFT and FRP for the bulk synthesis of acrylic and methacrylic molecularly imprinted polymers.. Polymer Chem., 2014, 5,1313-1322.
Bricks T., Paullier P., Legendre A., Fleury M. J., Zeller P., Merlier F., Anton P. M. & Leclerc E. Development of a new microfluidic platform integrating co-cultures of intestinal and liver cell lines.. Toxicol. In Vitro, 2014, 28,885-895.
Vidal G., Blanchi T., Mieszawska A. J., Calabrese R., Rossi C., Vigneron P., Duval J. L., Kaplan D. L. & Egles C. Enhanced cellular adhesion on titanium by silk functionalized with titanium binding and RGD peptides.. Acta Biomater., 2013, 9,4935-4943.
Cakir P., Cutivet A., Resmini M., Tse Sum Bui B. & Haupt K. Protein-size molecularly imprinted polymer nanogels as synthetic antibodies, by localized polymerization with multi-initiators.. Adv. Mater., 2013, 25,1048-1051.
Koubaa M., Cocuron J. C., Thomasset B. & Alonso A. Highlighting the tricarboxylic acid cycle : liquid and gas chromatography-mass spectrometry analyses of 13C-labeled organic acids.. Anal. Biochem., 2013, 436,151-159.
Ton X. A., Tse Sum Bui B., Resmini M., Bonomi P., Dika I., Soppera O. & Haupt K. A versatile fiber-optic fluorescence sensor based on molecularly imprinted microstructures polymerized in situ.. Angew. Chem. Int. Ed., 2013, 52,8317-8321.
Ben Naya R., Matti K., Guellier A., Matagne A., Boquet D., Thomas D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Efficient refolding of a recombinant abzyme: Structural and catalytic characterizations.. Appl. Microbiol. Biotechnol., 2013, 97,7721-7731.
Chantreau M., Grec S., Gutierrez L., Dalmais M., Pineau C., Demailly H., Paysant-leroux C., Tavernier R., Trouve J. P., Chatterjee M., Guillot X., Brunaud V., Chabbert B., Van Wuytswinkel O., Bendahmane A., Thomasset B. & Hawkins S. PT-Flax (phenotyping and TILLing of flax): development of a flax (Linum usitatissimum L.) mutant population and TILLing platform for forward and reverse genetics.. BMC Plant Biol., 2013, 13,159-174.
Fenart S., Chabi M., Gallina S., Huis R., Neutelings G., Riviere N., Thomasset B., Hawkins S. & Lucau-danila A. Intra-platform comparison of 25-mer and 60-mer oligonucleotide Nimblegen DNA microarrays.. BMC Res. Notes, 2013, 6,43.
Smirnov I., Belogurov A., Friboulet A., Masson P., Gabibov A. G. & Renard P. Y. Strategies for the selection of catalytic antibodies against organophosphorus nerve agents.. Chem-Biol. Interact., 2013, 203,196-201.
Almant M., Mastouri A., Gallego-yerga L., Garcia-fernandez J. M., Ortiz-mellet C., Kovensky J., Morandat S., El Kirat K. & Gouin S. G. Probing th nature of the cluster effect observed with synthetic multivalent galactosides and PNA lectin.. Chem.-Eur. J., 2013, 19,729-738.
Zdunek J., Benito-pena E., Linares A., Falcimaigne-cordin A., Orellana G., Haupt K. & Moreno-bondi M. C. Surface-imprinted nanofilaments for Europium-amplified luminescent detection of fluoroquinolones antibiotics.. Chem.-Eur. J., 2013, 19,10209-10216.
Chaveriat L., Gosselin I., Machut C. & Martin P. Synthesis, surface tension properties and antibacterial activities of amphiphilic D-galactopyranose derivatives.. Eur. J. Med. Chem., 2013, 62,177-186.
Belogurov A. A., Stepanov A. V., Smirnov I. V., Melamed D., Bacon A., Mamedov, A. E., Boltsov V. M., Sashchenko L. P., Ponomarenko N. A., Sharanova S. N., Boyko A. N., Dubina M. V., Friboulet A., Genkin D. D. & Gabibov A. G. Liposome-encapsulated peptides protect against experimental allergic encephalitis.. FASEB J. , 2013, 27,222-231.
Doyen A., Husson E. & Bazinet L. Use of an electrodialytic reactor for the simultaneous ß-lactoglobulin enzymatic hydrolysis and fractionation of generated bioactive peptides.. Food Chem., 2013, 136,1193-1202.
Husson E., Araya-farias M., Dejardins Y. & Bazinet L. Selective anthocyanins enrichment of cranberry juice by electrophoresis with multiple ultrafiltration membranes stacked.. Innov. Food Sci. Emerg. Technol. , 2013, 17,153-162.
Wattraint O., Saadallah I., Silva-peres V., Sonnet P. & Sarazin C. Influence of the insertion of a cationic peptide on the size and shape of nanoliposomes: a light scattering investigation.. Int. J. Pharm., 2013, 454,621-624.
Brissonnet Y., Ortiz-mellet C., Morandat S., Garcia-moreno M. I., Deniaud D., Matthews S. E., Vidal S., Sestak S., El Kirat K. & Gouin S. G. Topological effects and binding modes operating with multivalent imino-sugar-based glycoclusters and mannosidases.. J. Am. Chem. Soc., 2013, 135,18427-18435.
Husson E., Araya-farias M., Gagne A. & Bazinet L. Selective anthocyanins enrichment of cranberry juice by electrodialysis with filtration membranes: influence of membrane characteristics.. J. Membrane Sci., 2013, 448,114-124.
Dmitrienko E. V., Bulushev R. D., Haupt K., Kosolobov S. S., Latyshev A. V., Pyshnaya I. A. & Pyshnyi D. V. A simple approach to prepare molecularly imprinted polymers from nylon-6.. J. Mol. Recognit., 2013, 26,368-375.
Fang J., Ramsay A., Paetz C., Tatsis E. C., Renouard S., Hano C., Grand E., Fliniaux O., Roscher A., Mesnard F. & Schneider B. Concentration kinetics of secoisolariciresinol diglucoside and its biosynthetic precursor coniferin in developing flaxseed.. Phytochem. Anal., 2013, 24,41-46
Mahendra A., Peyron I., Dollinger C., Gilardin L., Sharma M., Wootla B., Padiolleau-lefevre S., Friboulet A., Boquet D., Legendre C., Kaveri S., Thaunat O. & Lacroix-desmazes S. IVIg treatment reduces catalytic antibody titers of renal transplanted patients.. PloS One, 2013, 8,e70731.
Veneziano R., Rossi C., Chenal A., Devoiselle J. M., Ladant D. & Chopineau J. Bordetella pertussis Adenylate Cyclase Toxin Translocation across a Tethered Lipid Bilayer.. Proc. Natl. Acad. Sci. USA, 2013, 110,20473-20478.
Kunath S., Marchyk N., Haupt K. & Feller K. H. Multi-objective optimization and design of experiments as tools to tailor molecularly imprinted polymers specific for glucuronic acid.. Talanta, 2013, 105,211-218.
Gervaise C., Bonnet V., Wattraint O., Aubry F., Sarazin C., Jaffres P. A. & Djedaini-pilard F. Synthesis of lipophosphoramidyl-cyclodextrins and their supramolecular properties.. Biochimie, 2012, 94,66-74.
Ton X. A., Acha V., Haupt K. & Tse Sum Bui B. Direct fluorimetric sensing of UV-excited analytes in biological and environmental samples using molecularly imprinted polymer nanoparticles and fluorescence polarization.. Biosens. Bioelectron., 2012, 36,22-28.
Mahendra A., Padiolleau-lefevre S., Kaveri S. & Lacroix-desmazes S. Do proteolytic antibodies complete the panopply of the autoimmune response in acquired haemophilia A?. Br. J. Haematol., 2012, 156,3-12.
Auxenfans T., Buchoux S., Djellab K., Avondo C., Husson E. & Sarazin C. Mild pretreatment and enzymatic saccharification of cellulose with recycled ionic liquid towards one-batch process.. Carbohydr. Polym., 2012, 90,805-813.
Bernier M. C., El Kirat K., Besse M., Morandat S. & Vayssade M. Preosteoblasts and fibroblasts respond differently to anastase titanium dioxide nanoparticles: a cytotoxicity and inflammatory study.. Colloids Surf. B, 2012, 90,68-74.
Dufourc E. J., Buchoux S., Toupe J., Sani M. A., Jean-francois F., Khemtemourian L., Grelard A., Loudet_courreges C., Laguerre M., Elezgaray J., Desbat B. & Odaert B. Membrane interacting peptides: from killers to helpers.. Curr. Prot. Pept. Sci., 2012, 13,620-631.
Rosant C., Avalle B., Larcher D., Dupont A., Friboulet A. & Tarascon J. M. Biosynthesis of electrode nanomaterials by phage engineering: comprehension and future.. Energ. Environ. Sci., 2012, 5,9936-9943.
Bernier M. C., Besse M., Vayssade M., Morandat S. & El Kirat K. Titanium dioxide nanoparticles disturb the fibronectin-mediated adhesion and spreading of pre-osteoblastic cells.. Langmuir, 2012, 28,13660-13667.
Boussetta N., Vorobiev E., Le L. H., Cordin-falcimaigne A. & Lanoiselle J. L. Application of electrical treatments in alcoholic solvent for polyphenols extraction from grape seeds.. LWT-Food Sci. Technol., 2012, 46,127-134.
Le Minoux D., Mahendra A., Lacroix-desmazes S., Limnios N., Friboulet A., Avalle B., Boquet D., Kaveri S. & Padiolleau-lefevre S. A novel molecular analysis of genes encoding catalytic antibodies.. Mol. Immunol., 2012, 50,160-168.
Rippa S., Zhao Y., Merlier F., Charrier A. & Perrin Y. The carnitine biosynthetic pathway in Arabidopsis thaliana shares similar features with the pathway of mamals and fungi.. Plant Physiol. Biochem., 2012, 60,109-114.
Husson E., Buchoux S., Avondo C., Cailleu D., Djellab K., Gosselin I., Wattraint O. & Sarazin C. Enzymatic hydrolysis of ionic liquid-pretreated celluloses: contribution of CP-MAS, 13C NMR and SEM.. Bioresource Technol., 2011, 102,7335-7342.
Pilalis E., Chatziioannou A., Thomasset B. & Kolisis F. An In-silico compartmentalized, metabolic model of Brassica napus, enables the systemic study of regulatory aspects of plant central metabolism.. Biotechnol. Bioeng., 2011, 108,1673-1682.
Wootla B., Christophe O., Mahendra A., Dimitrov J., Repesse Y., Ollivier V., Friboulet A., Borel-derlon A., Levesque H., Borg J. Y., Andre S., Bayry J., Calvez T., Kaveri S. & Lacroix-desmazes S. Proteolytic antibodies activate factor IX in patients with acquired hemophilia. Blood, 2011, 117,2257-2264.
Fuchs Y., Linares A. V., Mayes A. G., Haupt K. & Soppera O. Ultrathin selective molecularly imprinted polymer microdots obtained by evanescent wave photopolymerization.. Chem. Mater., 2011, 23,3645-3651.
Harz S., Shimmelpfennig M., Tse Sum Bui B., Marchyk N., Haupt K. & Feller K. H. Fluorescence optical spectrally resolved sensor based on molecularly imprinted polymers and microfluidics.. Eng. Life Sci., 2011, 11,559-565.
Gabibov A. G., Belogurov A. A., Lomakin Y. A., Zakharova M. Y., Avakyan M. E., Dubrovskaya V. V., Smirnov I. V., Ivanov A. S., Molnar A. A., Gurtsevitch V. E., Diduk S. V., Smirnova K. V., Avalle B., Sharanova S. N., Tramontano A., Friboulet A., Boyko A. N., Ponomarenko N. A. & Tikumova N. V. Combinatorial antibody library from Multiple Sclerosis patients reveals antibodies that cross-react with myelin basic protein and EBV-antigen.. FASEB J., 2011, 25,4211-4221.
Boussetta N., Vorobiev E., Deloison V., Pochez F., Falcimaigne-cordin A. & Lanoiselle J. L. Valorisation of grape pomace by the extraction of phenolic antioxidants: Application of high voltage electrical discharges.. Food Chem., 2011, 128,364-370.
Tse Sum Bui B. & Haupt K. Preparation and evaluation of a molecularly imprinted polymer for the selective recognition of testosterone application to molecularly imprinted sorbent assays.. J. Mol. Recognit., 2011, 24,1123-1129.
Diot J. D., Moreno I. G., Twigg G., Ortiz Mellet C., Haupt K., Butters T. D., Kovensky J. & Gouin S. G. Amphiphilic 1-deoxynojirimycin derivatives through click strategies for chemical chaperoning in N370S Gaucher cells.. J. Org. Chem., 2011, 76,7757-7768.
Zakharov A. V., Smirnov I. V., Serebryakova M. V., Dronina M. A., Kaznacheeva A. V., Kurkova I. N., Belogurov A. A., Friboulet A., Ponomarenko N. A., Gabibov A. G. & Bobik T. V. Expression of catalytic antibodies in eukaryotic systems. Mol. Biol., 2011, 45,74-81.
Hegde P., Maddur M., Friboulet A., Bayry J. & Kaveri S. Viscum album exerts anti-inflammatory effect by selectively inhibiting cytokine-induced expression of cyclooxygenase-2.. PloS One, 2011, 6,e26312.
Prot J. M., Briffaut A. S., Letourneur F., Chafey P., Merlier F., Grandvalet Y., Legallais C. & Leclerc E. Integrated proteomic and transcriptomic investigation of the acetaminophen toxicity in liver microfluidic biochip.. PloS One, 2011, 6,e21268.
Rossi C., Doumiati S., Lazzarelli C., Davi M., Meddar F., Ladant D. & Chopineau J. A tethered bilayer assembled on top of immobilized calmodulin to mimic cellular compartmentalization.. PloS One, 2011, 6,e19101.
Stepanov A. V., Belogurov A. A., Ponomarenko N. A., Stremkovskly O. A., Kozlov L. V., Bichucher A. M., Dmitriev S. E., Shatsky I. N., Smirnov I. V., Shamborant O. G., Tonevitsky A. G., Friboulet A., Gabibov A. G. & Deyev S. M. Design of targeted B-cell killing agents.. PloS One, 2011, 6,e20991.
Smirnov I., Carletti E., Kurkova I., Nachon F., Nicolet Y., Mitkevich V., Debat H., Avalle B., Belogurov A., Kuznetsov N., Reshetnyak A., Masson P., Tonevitsky A., Ponomarenko N., Makarov A. A., Friboulet A., Tramontano A. & Gabibov A. G. Reactibodies generated by kinetic selection couple chemical reactivity with faborable protein dynamics.. Proc. Natl. Acad. Sci. USA, 2011, 108,15954-1959.
Linares A. V., Falcimaigne-cordin A., Gheber L. A. & Haupt K. Patterning nanostructured, synthetic, polymeric receptors by simultaneous projection photolithography, nanomolding, and molecular imprinting.. Small, 2011, 7,2318-2325.
Tse Sum Bui B., Merlier F. & Haupt K. Towards the use of a molecularly imprinted polymer in doping analysis :selective preconcentration and analysis of testosterone and epitestosterone in human urine.. Anal. Chem., 2010, 82,4420-4427.
Fenart S., Ndong Y. P., Duarte J., Riviere N., Van Wuytswinkel O., Lucau A., Cariou E., Neutelings G., Gutierrez L., Chabbert B., Guillot X., Tavernier R., Hawkins S. & Thomasset B. Development and validation of a flax (Linum usitatissinum L.) gene expression oligo microarray.. BMC Genomics, 2010, 11,592.
Jousse C., Schiltz S., Fournez A., Guillot X., Thomasset B., Gougeon S., Bourgaud F. & Gontier E. Rapid, cost-effective screening of flax genotypes to identify desirable fatty acid compositions.. Electron. J. Plant Breed., 2010, 1,1396-1404
Phichith D., Bun S., Padiolleau-lefevre S., Guellier A., Banh S., Gallieni M., Frere J. M., Thomas D., Friboulet A. & Avalle B. Novel peptide inhibiting both TEM-1 beta-lactamase and penicillin-binding proteins.. FEBS J., 2010, 277,4965-4972.
Dehkharghanian M., Adenier H. & Vijayalakshmi M. A. Study of flavonoids in aqueous spinach extract using positive electrospray ionisation tandem quadrupole mass spectrometry.. Food Chem., 2010, 121,863-870.
Batchelor R., Windle C. J., Buchoux S. & Lorch M. Cholesterol and lipid phases influence the interactions between serotonin receptor agonists and lipid bilayers.. J. Biol. Chem., 2010, 285,41402-41411.
Eid M., Rippa S., Castano S., Desbat B., Chopineau J. Rossi C. & Beven L. Exploring the membrane mechanism of the bioactive peptaibol ampullosporin A using lipid monolayers and supported biomimetic membranes. J. Biophys., 2010, 2010,Art.ID 179641.
Favrelle A., Bonnet V., Avondo C., Aubry F., Djedaini-pilard F. & Sarazin C. Lipase-catalyzed synthesis and characterization of novel lipidyl-cyclodextrins in solvent free medium.. J. Mol. Catal. B., 2010, 66,224-227.
Laveille P., Falcimaigne A., Chamouleau F., Renard G., Drone J., Fajula F., Pulvin S., Bedel-cloutour C., Thomas D., Bailly C. & Galarneau A. Hemoglobin immobilized on mesoporous silica as effective material for the removal of polycyclic aromatic hydrocarbons pollutants from water.. New J. Chem., 2010, 34,2153-2165.
Claude B., Viron-lamy C., Haupt K. & Morin P. Synthesis of a molecularly imprinted polymer for the solid-phase extraction of betulin and betulinic acid from plane bark.. Phytochem. Anal., 2010, 21,180-185.
Essono S., Clement G., Padiolleau-lefevre S., Creminon C., Grassi J. & Boquet D. Peptide mass assisted antibody cloning strategy for accurate characterization of potential therapeutic monoclonal antibodies against neurodegenerative diseases.. Prot. Eng. Des. Select., 2010, 23,203-210.
Linares A. V., Vandevelde F., Pantigny J., Falcimaigne-cordin A. & Haupt K. Polymer films composed of surface-bound nanofilaments with a high aspect ratio, molecularly imprinted with small molecules and proteins.. Adv. Funct. Mater., 2009, 19,1299-1303.
Kantarovich K., Tsarfati I., Gheber L. A., Haupt K. & Bar I. Writing droplets of molecularly imprinted polymers by nano fountain pen and detecting their molecular interactions by surface-enhanced Raman scattering.. Anal. Chem., 2009, 81,5686-5690.
Elluru S. R., Duong Van Huyen J. P., Delignat S., Prost F., Heudes D., Kazatchkine M. D., Friboulet A. & Kaveri S. V. Antiangiogenic properties of viscum album extracts are associated with endothelial cytotoxicity.. Anticancer Res., 2009, 29,2945-2950
Lotierzo M., Tse Sum Bui B., Leech H. K., Warren M. J., Marquet A. & Rigby S. E. J. Iron-sulfur cluster dynamics in biotin synthase: A new [2Fe-2S]1+ cluster.. Biochem. Biophys. Res. Commun. , 2009, 381,487-490.
Nlandu Mputu M., Rhazi L., Vasseur G., Vu T. D., Gontier E. & Thomasset B. Identification of a potential bottleneck in branched chain fatty acid incorporation into triacylglycerol for lipid biosynthesis in agronomic plants.. Biochimie, 2009, 91,703-710.
Bompart M., Geher L. A., De Wilde Y. & Haupt K. Direct detection of analyte binding to single molecularly imprinted polymer particles by confocal Raman spectroscopy.. Biosens. Bioelectron., 2009, 25,568-571.
Pasetto P., Flavin K. & Resmini M. Simple spectroscopic method for titration of binding sites in molecularly imprinted nanogels with hydrolase activity.. Biosens. Bioelectron., 2009, 25,572-578
Laveille P., Galarneau A., Drone J., Fajula F., Bailly C., Pulvin S. & Thomas D. Catalase-like activity of bovine met-hemoglobin: interaction with the pseudo-catalytic peroxidation of anthracene traces in aqueous medium.. Biotechnol. J., 2009, 4,1460-1470.
Wootla B., Desirazu N. R., Friboulet A., Uda T., Lacroix-desmazes S. & Kaveri S. V. Varied immune response to FVIII: Presence of proteolytic antibodies directed to Factor VIII in different human pathologies.. Clin. Rev. Allerg. Immunol., 2009, 37,97-104.
Kurkova I. N., Reshetnyak A. V., Durova O. M., Knorre V. D., Tramontano A., Friboulet A., Ponomarenko N. A., Gabibov A. G. & Smirnov I. V. Antibodes-antidotes against organophosphorus compounds?. Dokl. Biochem. Biophys., 2009, 425,94-97.
Landoulsi J., Dagbert C., Richard C., Sabot R., Jeannin M., El Kirat K. & Pulvin S. Enzyme-induced ennoblement of AISI 316L stainless steel: Focus on pitting corrosion behavior.. Electrochim. Acta, 2009, 54,7401-7406.
Tanvir S., Adenier H. & Pulvin S. Screening and prediction of reactive intermediates in a microreactor with immobilized rat hepatic microsomes using acetaminophen as a model drug. . Enzyme Microbiol. Technol., 2009, 45,112-117.
Wootla B., Mahendra A., Dimitrov J. D., Friboulet A., Borel-derlon A., Rao D. N., Uda T., Borg J. Y., Bayry J., Kaveri S. V. & Lacroix-desmazes S. Factor VIII-hydrolyzing IgG in acquired and congenital hemophilia.. FEBS Letters, 2009, 583,2565-2572.
Boussetta N., Lebovka N., Vorobiev E., Adenier H., Bedel Cloutour C. & Lanoiselle J. L. Electrically assisted extraction of soluble matter from chardonnay grape skins for polyphenol recovery.. J. Agr. Food Chem., 2009, 57,1491-1497.
Mhaka B., Cukrowska E., Tse Sum Bui B., Ramstrom O., Haupt K., Tutu H. & Chimuka L. Selective extraction of triazine herbicide from food samples based on a combination of a liquid membrane and molecularly imprinted polymers.. J. Chromatogr. A, 2009, 1216,6796-6801.
Boussetta N., Lanoiselle J. L., Bedel-cloutour C. & Vorobiev E. Extraction of soluble matter from grape pomace by high voltage electrical discharges for polyphenol recovery: Effect of sulphur dioxide and thermal treatments.. J. Food Eng., 2009, 95,192-198.
Shynkaryk M. V., Lebovska N. I., Lanoiselle J. L., Nonus M., Bedel-cloutour C. & Vorobiev E. Electrically-assisted extraction of bio-products using high pressure disruption of yeast cells (Saccharomyces cerevisiae).. J. Food Eng., 2009, 92,189-195.
Tanvir S., Pantigny J., Boulnois P. & Pulvin S. Covalent immobilization of recombinant human cytochrome CYP2E1 and glucose-6-phosphate dehydrogenase in alumina membrane for drug screening applications.. J. Membrane Sci., 2009, 329,85-90.
Kouzayha A., Nasir M. N., Buchet R., Wattraint O., Sarazin C. & Besson F. Conformational and interfacial analyses of K3A18K3 and alamethicin in model membranes.. J. Phys. Chem. B, 2009, 113,7012-7019.
Pasetto P., Blas H., Audouin F., Boissiere C., Sanchez C., Save M. & Charleux B. Mechanistic insight into surface-initiated polymerization of methylmetacrylate and styrene via ATRP from ordered mesoporous silica particles.. Macromolecules, 2009, 42,5983-5995
Durova O. M., Vorobiev I. I., Smirnov I. V., Reshetnyak A. V., Telegin G. B., Shamborant O. G., Orlova N. A., Genkin D. D., Bacon A., Ponomarenko N. A., Friboulet A. & Gabibov A. G. Strategies for induction of catalytic antibodies towards HIV-1 glycoprotein gp120 in autoimmune prone mice.. Mol. Immunol., 2009, 47,87-95.
Phichith D., Bun S., Padiolleau-lefevre S., Banh S., Thomas D., Friboulet A. & Avalle B. Mutational and inhibitory analysis of a catalytic antibody. Implication for drug discovery.. Mol. Immunol., 2009, 47,348-356.
Labboun S., Terce-laforgue T., Roscher A., Bedu M., Restivo F. M., Velanis C. N., Skopelitis D. S., Moshou P. N., Roubelakis K. A., Suzuki A. & Hirel B. Resolving the role of plant glutamate dehydrogenase: I. In vivo real time nuclear magnetic resonance spectroscopy experiments.. Plant Cell Physiol., 2009, 50,17761-1773.
Picard N., Guenin S., Perrin Y., Hilaire G. & Larnicol N. Consequences of prenatal exposure to diazepam on the respiratory parameters, respiratory network activity and gene expression of a1 and alpha-2 subunits of GABA(A) receptor in newborn rat.. Adv. Exp. Biol. Med., 2008, 605,144-148.
Tse Sum Bui B., Belmont A. S., Witters H. & Haupt K. Molecular recognition of endocrine disruptors by synthetic and natural 17beta-estradiol receptors: a comparative study.. Anal. Bioanal. Chem., 2008, 390,2081-2088.
Elluru S. R., Vani J., Delignat S., Bloch M. F., Lacroix-desmazes S., Kazatchkine M. D., Kaveri S. V. & Bayry J. Modulation of human dendritic cell maturation and function by natural IgG antibodies.. Autoimmun. Rev., 2008, 7,487-490.
Rohde M. & Stewart J. Ascriptional and "genuine" autonomy.. BioSystems, 2008, 9,424-433
Noizet M., Harrabi F., Vijayalakshmi M., Galbraith D., Thomas D. & Thomasset B. Targeted protein accumulation promoted by autoassembly and its recovery from plan cells.. Biotechnol. J., 2008, 3,392-402.
Elluru S. R., Duong Van Huyen J. P., Delignat S., Kazatchkine M. D., Friboulet A., Kaveri S. V. & Bayry J. Induction of maturation and activation of human dendritic cells: A mechanism underlying the beneficial effect of Viscum album as complimentary therapy in cancer.. BMC Cancer, 2008, 8,161.
Smirnov I. V., Vorobiev I. I., Friboulet A., Avalle B., Thomas D., Knorre V. D., Gabibov A. G. & Ponomarenko N. A. The antiidiotypic approach to obtaining a proteolytic antibody.. Dokl. Biochem. Biophys., 2008, 420,105-107.
Landoulsi J., El Kirat K., Richard C., Sabot R., Jeannin M. & Pulvin S. Glucose oxidase immobilization on stainless steel to mimic the aerobic activities of natural biofilms.. Electrochim. Acta, 2008, 54,133-139.
Landoulsi J., El-kirat K., Richard C., Feron D. & Pulvin S. Enzymatic approach in microbial influenced corrosion : A review based on stainless steels in natural waters.. Env. Sci. Technol., 2008, 42,2233-2242.
Landoulsi J., Genet M. J., Richard C., El Kirat K., Pulvin S. & Rouxhet P. G. Evolution of the passive film and organic constituents at the surface of stainless steel immersed in fresh water.. J. Colloid Interf. Sci., 2008, 318,278-289.
Landoulsi J., Genet M. J., Richard C., El Kirat K., Rouxhet P. G. & Pulvin S. Ennoblement of stainless steel in the presence of glucose oxidase: nature and role of interfacial processes.. J. Colloid Interf. Sci., 2008, 320,508-519.
Belogurov A. A., Kurkova I. N., Friboulet A., Thomas D., Misikov V. K., Zakharova M. U., Suchkov S. V., Kotov V., Alexin A. I., Avalle B., Souslova E. A., Morse H. C., Gabibov A. G. & Ponomarenko N. A. Recognition and Degradation of Myelin Basic Protein Peptides by Serum Autoantibodies as a Novel Biomarker for Multiple Sclerosis.. J. Immunol., 2008, 180,1258-1267.
Wootla B., Dasgupta S., Dimitrov J. D., Bayry J., Levesque H., Borg J. Y., Borel-derlon A., Rao D. N., Friboulet A., Kaveri S. V. & Lacroix-desmazes S. FVIII hydrolysis mediated by anti-FVIII autoantibodies in acquired hemophilia.. J. Immunol., 2008, 180,7714-7720.
Wootla B., Nicoletti A., Patey N., Dimitrov J. D., Legendre C., Christophe O. D., Friboulet A., Kaveri S. V., Lacroix-desmazes S. & Thaunat O. Hydrolysis of coagulation factors by circulating IgG is associated with a reduced risk for chronic allograft nephropathy in renal transplanted patients.. J. Immunol., 2008, 180,8455-8460.
Picard N., Guenin S., Perrin Y. & Larnicol N. Maternal caffeine ingestion during gestation and lactation influences respiratory adaptation to acute alveolar hypoxia in newborn rats and adenosine A2A and GABA A receptor mRNA transcription.. Neuroscience, 2008, 156,630-639.
Picard N., Guenin S., Perrin Y., Hilaire G. & Larnicol N. Prenatal diazepam exposure alters respiratory control system and Gabaa and adenosine receptor gene expression in newborn rats.. Pediatr. Res., 2008, 64,44-49.
Rossi C. & Haupt K Application of the Doehlert experimental design to molecularly imprinted polymers: surface response optimization of specific template recognition as a function of the type and degree of cross-linking.. Anal. Bioanal. Chem., 2007, 389,455-460.
Ponomarenko N. A., Pillet D., Paon M., Vorobiev I. I., Smirnov I. V., Adenier H., Avalle B., Kolesnikov A. V., Kozyr A. V., Thomas D., Gabibov A. G. & Friboulet A. Anti-idiotypic antibody mimics proteolytic function of parent antigen.. Biochemistry , 2007, 46,14598-14609.
Belmont A. S., Jaeger S., Knopp D., Niessner R., Gauglitz G. & Haupt K. Molecularly imprinted polymer films for reflectometric interference spectroscopic sensors.. Biosens. Bioelectron., 2007, 22,3267-3272.
Reshetnyak A. V., Armentano M. F., Morse H. C., Friboulet A., Makker S. P., Tramontano A., Knorre V. D., Gabibov A. G. & Ponomarenko N. A. Mechanism-dependent selection of immunoglobulin gene library for obtaining covalent biocatalysis.. Dokl. Biochem. Biophys., 2007, 415,179-182.
Reshetnyak A. V., Armentano M. F., Ponomarenko N. A., Durova O., Ziganshin R., Serebryakova M., Govorun V., Gololobov G., Morse H. C., Friboulet A., Makket S. P. Gabibov A. G. & Tramontano A. Route to covalent catalysis by reactive selection for nascent protein nucleophile.. J. Am. Chem. Soc., 2007, 129,16175-16182.
Deniaud A., Rossi C., Berquand A., Homand J., Campagna S., Knoll W., Brenner C. & Chopineau J. Voltage-dependent anion channel transports calcium ions through biomimetic membranes.. Langmuir, 2007, 23,3898-3905.
Troufflard S., Roscher A., Thomasset B., Barbotin J. N., Rawsthorne S. & Portais J. C. In vivo (13)C NMR determines metabolic fluxes and steady state in linseed embryos.. Phytochem., 2007, 68,, 2341-2350.
Robins R. J., Molinie R., Kwiecien R. A., Paneth P., Lebreton J., Bartholomeusz T. A., Roscher A., Drager B. & Mesnard F. Progress in understanding the N-demethylation of alkaloids by exploiting isotopic techniques. . Phytochem. Rev., 2007, 6,51-63.
Lelandais-briere C., Jovanovic M., Torres G. A., Perrin Y., Lemoine R., Corre-menguy F. & Hartmann C. Disruption of AtOCT1, an organic cation transporter gene, affects root development and carnitine-related responses in Arabidopsis.. Plant J., 2007, 51,154-164.
Sineriz F., Ikeda Y., Petit E., Bultel L., Haupt K., Kovensky J. & Papy-garcia D. Toward an alternative for specific recognition of sulfated sugars. Preparation of highly specific molecular imprinted polymers.. Tetrahedron, 2007, 63,1857-1862.
Delattre C., Michaud P., Keller C., Elboutachfaiti R., Beven L., Courtois B. & Courtois J. Purification and characterization of a novel glucuronan lyase from Trichoderma sp GL2. . Appl. Microbiol. Biotechnol., 2006, 70,437-443.
Ponomarenko N. A., Vorobiev I. I., Alexandrova E. S., Reshetnyak A. V., Telegin G. B., Khaidurov S. V., Avalle B., Karavanov A., Morse H. C., Thomas D., Friboulet A. & Gabibov A. G. Induction of protein-targeted catalytic response in autoimmune prone mice: antibody-mediated cleavage of HIV-1 glycoprotein gp120. . Biochemistry, 2006, 45,324-330.
Alamillo J. M., Monger W., Sola I., Garcia B., Perrin Y., Bestagno M., Burrone O. R., Plana-duran J., Enjuanes L., Lomonossoff G. P. & Garcia J. A. Use of virus vectors for the expression in plants of active full-length and single-chain anti-coronavirus antibodies. . Biotechnol. J., 2006, 1,1103-1111.
Wootla B., Dasgupta S., Mallet V. O., Kazatchkine M. D., Nagaraja V., Friboulet A., Kaveri S. V. & Lacroix-desmazes S. Physiopathology of catalytic antibodies: the case for FVIII-hydrolyzing IgG. . Blood Coagul. Fibrin., 2006, 17,229-234.
Padiolleau-lefevre S., Debat H., Phichith D., Thomas D., Friboulet A. & Avalle B. Expression of a functional scFv fragment of an anti-idiotypic antibody with a ß-lactam hydrolytic activity. . Immunol. Letters, 2006, 103,39-44.
Gutierrez L., Conejero G., Castelain M., GuÉnin S., Verdeil J. L., Thomasset B. & Van Wuytswinkel O. Identification of new gene expression regulators specifically expressed during plant seed maturation.. J. Exp. Bot., 2006, 57,1919-1932.
Lacroix-desmazes S., Wootla B., Dasgupta S., Delignat S., Bayry J., Reinbold J., Hoebeke J., Saenko E., Kazatchkine M. D., Friboulet A., Christophe O., Nagaraja V. & Kaveri S. N. Catalytic IgG from patients with hemophilia A inactivate therapeutic factor VIII. . J. Immunol., 2006, 177,1355-1363.
Canizares M. C., Liu L., Perrin Y., Tsakiris E. & Lomonossoff G. P. A bipartite system for the constitutive and inducible expression in high levels of foreign proteins in plants. . Plant Biotechnol. J., 2006, 4,183-193.
Monger W., Alamillo J. M., Sola I., Perrin Y., Bestagno M., Burrone O. R., Sabella P., Plana-duran J., Enjuanes L., Garcia J. A. & Lomonossoff G. P. An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus infection when supplied orally in crude plant extracts. . Plant Biotechnol. J., 2006, 4,623-631.
Ponomarenko N. A., Durova O. M., Vorobiev I. I., Belogurov A. A., Telegin G. B., Suchkov S. V., Misikov V. K., Kiselev S. L., Lagarkova M. A., Govorun V. M., Serebryakova M. V., Avalle B., Tornatore P., Karavanov A., Morse H. C., Thomas D., Friboulet A. & Gabibov A. G. Autoantibodies to myelin basic protein catalyze site specific degradation of their antigen. . Proc. Natl. Acad. Sci. USA, 2006, 103,281-286.
Bokeloh F., Ayela C. & Haupt K. Micro and nanofabrication of molecularly imprinted polymers . Molecularly Imprinted Polymers for Analytical Chemistry Applications , 2018, W. Kutner and P. Sindhu Sharma,pp. 167-197
Xu J, Medina-rangel P. X., Haupt K. & Tse Sum Bui B. Guide to the preparation of molecularly imprinted polymer nanoparticles for protein recognition, by solid-phase synthesis. Methods Enzymol, 2017, ,pp.115-141
Panagiotopoulou M., Kunath S., Haupt K., Tse Sum Bui B. Cell and Tissue Imaging with Molecularly Imprinted Polymers . Methods in Molecular Biology, 2017, ,PP.399-415
Saha C., Stephen-victor E., Friboulet A., Bayry J. & Kaveri S. V. Viscum album and its anti-inflammatory effect: angle of cytokine induced selective inhibition of COX-2.. Die Mistel in der Tumortherapie 4, 2016, S. Scheer, A. Alban et al. eds.,pp. 197-203
Chavelot I, Arab-tehrany E, Husson E, Gerardun C, Vandamme E, Luis Revuelta J. Application of carnosine and its functionalised derivatives. Industrial Biotechnology of vitamins, biopigments, and antioxidants , 2016, E.J. Vandamme & J.L. Revuelta eds. ,pp. 421-444
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E. Leclerc, P. Paullier, F. Merlier. Facility for coupling a bioreactor with a device for physicochemically analysing or collecting samples. , 2014, ,. ABSTRACT: The invention concerns a facility for coupling a bioreactor for culturing bio-organisms, in dynamic mode, with at least one device for physicochemically analyzing a fluid from this bioreactor or collecting samples of this fluid, the device being equipped with a pump and an injector, the bioreactor being equipped with means for supplying culture medium and xenobiotic(s). The facility is remarkable in that the bioreactor is connected to a closed perfusion loop provided with a pump, fitted with a sampling loop and in that the facility comprises: a device for injecting a cleaning product into said sampling loop, two six-way valves, the first valve being connected to the closed perfusion loop, to the sampling loop and to a cleaning loop, the second valve being connected to the cleaning loop and an injection loop that connects the injector to at least one of the devices.