PhD students and Post-docs

Thibault ANNAVAL

ATER UPJV

Research

I’m working at the interface between the two themes and under the supervision of Pr N. d’Amelio.

My main research topics are:

  • Development of a web server referencing data on antimicrobial peptides in order to guide the screening of optimal sequences of peptides capable of high antimicrobial resistance.
  • Structure determination of antimicrobial peptides free or bound in bicelles as models of biological membranes.

 

Skills

Biochemistry, enzymology, structural biology, natural products, produits naturels, polyketides

 

General information

  • 2018-2019: ATER – GEC UMR 7025 CNRS/Université de Picardie Jules Verne, Amiens, France
  • 2016-2018: Post-doctorat – The Scripps Research Institute, Jupiter, Florida, USA
  • 2012-2015: Doctorat – IMoPA UMR 7365 CNRS/Université de Lorraine, Nancy, France

 

Main publications

Articles

 

 

Ranim AL AYOUBI

PhD student UPJV

Research

Member of the theme “Plant Metabolism and Bioresources”.

PhD project: Study of ethanol fermentation by yeasts of enzymatic hydrolysates of lignocellulosic substrates pretreated with ionic liquids (under the supervision of Pr C. Sarazin).

 

Skills

Enzymology, microbiology and pretreatment of lignocellulosic biomass by ionic liquids.

 

General information

  • Biochemistry licence, Lebanon
  • Applied biotechnology master, Lebanon
  • Biohemistry and enzymatic engineering PhD – in progress (Université de Picardie Jules Verne – Université Libanaise)

 

Personal interests

Swimming, hiking.

 

Claudia HERRERA LEON

PhD student UPJV
Research

I’m working at the interface between the two themes and working under the supervision of Pr N. d’Amelio.

Main research topics:

  • Design and physicochemical characterization of antimicrobial peptides: NMR study of their interaction with the biomimetic membranes of cancer cells by NMR.
  • PhD project: Design and testing of selectivity and haemolytic properties of novel antimicrobial peptides based on sequence alignments (under the supervision of Pr N. d’Amelio).

 

Skills

Biochemistry, microbiology, molecular biology.

 

General information

  • Licence in pharmaceutical chemistry and biology, Veracruzana university (Mexico)
  • Master in Biotechnology, Meritorious Autonomous University of Puebla (Mexico)
  • PhD, Université de Picardie Jules Verne (Amiens, France)

 

Personal interests

Music, stand up paddle

Noadya MONNIER

Post doctoral student UTC

Research

N. Monnier is currently a post-doctoral fellow in the theme “Plant Metabolism and Bioresources”, under the scientific supervision of Dr S. Rippa.

Rhamnolipids produced by Pseudomonas aeruginosa are glycolipids with both antimicrobial and plant defense triggering activities. N. Monnier’s work focuses on rhamnolipids mode of action on both plants and fungi. It combines in vivo studies with a biophysical approach on biomimetic membrane models. Indeed, rhamnolipid amphiphilic properties have raised the possibility of a direct interaction with the lipidic fraction of plant and fungal plasma membranes.

 

Skills

  • Plant defense studies through microscopic observations (stomata opening, callose deposition) and gene expression quantification (qPCR, transcriptomics) on Brassicaceae (A. thaliana, B. napus).
  • Study of antifungal activities in culture medium (inhibition of mycelial growth and spore germination) and on foliar tissue.
  • Study of amphiphilic compounds interaction with biomimetic membranes (2H and 31P solid-state NMR, FTIR, ITC…).

 

General information

After a broad based education (engineering degree in Centrale Marseille and master degree in chemistry in Aix Marseille Université), N. Monnier did her PhD in GEC – UPJV under the supervision of Pr C. Sarazin and Dr S. Rippa (December 2014 – February 2018). Her work, founded by the Hauts de France council and the ERDF, focused on plant defences and in particular on the perception and mode of action of natural glycolipids, rhamnolipids, triggering plant innate immunity. Between September 2017 and June 2018, as a research and teaching assistant in GEC –UPJV, she studied rhamnolipids antifungal activities. She is currently a post doctoral researcher in the theme “Plant Metabolism and Bioresources” in GEC – UTC, under the scientific supervision of Dr S. Rippa and founded by PIVERT (EliZa project). Her work aim to explore rhamnolipid potential as a biocontrol agent.

 

Projects

Post doc project: Optimisation of rhamnolipid mixes whith antinfungal and eliciting activities for protection of rapeseed and other oleaginous brassicaceae.

Future projects: Public or private research on plant biology or biocontrol.
 

Main publications

Articles

Monnier N., Furlan A-l., Buchoux S., Deleu M., Dauchez M., Rippa S., Sarazin C. Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies" has been published in IJMS as part of the Special Issue SAR "Out of the Box. Int. J. Mol. Sci.2019, 20,1009.
 

Monnier M., Furlan A., Botcazon C., Dahi A., Mongelard G., Cordelier S., Clement C., Dorey S., Sarazin C., Rippa S. Rhamnolipids from Pseudomonas aeruginosa are elicitors triggering Brassica napus protection against Botrytis cinerea without physiological disorders. Frontiers in Plant Science2018, 1778,101-124.
     ABSTRACT: Rhamnolipids (RLs) are amphiphilic molecules naturally produced by some bacteria with a large range of biological activities. Although some studies report their potential interest in plant protection, evaluation of their effects and efficiency on annual crops of worldwide agronomic interest is lacking. The main objective of this work was to investigate their elicitor and protective activities on rapeseed crop species while evaluating their physiological effects. Here we report that RLs from Pseudomonas aeruginosa secretome trigger an effective protection of Brassica napus foliar tissues toward the fungus Botrytis cinerea involving the combination of plant defense activation and direct antimicrobial properties. We demonstrated their ability to activate canonical B. napus defense responses including reactive oxygen species production, expression of defense genes, along with callose deposits and stomatal closure as efficient physical protections. In addition, microscopic cell death observations and electrolyte leakage measurements indicated that RLs trigger a hypersensitive response-like defense in this plant. We also showed that foliar spray applications of RLs do not induce deleterious physiological consequences on plant growth or chlorophyll content and that RL protective properties are efficient on several grown cultivars of rapeseed. To our knowledge, this is the first report of RLs as an elicitor that suppresses fungal disease on tissues of an annual crop species under greenhouse conditions. Our results highlight the dual mode of action of these molecules exhibiting plant protection activation and antifungal activities and demonstrate their potential for crop cultures as environmental-friendly biocontrol solution.

Salim MAKNI

PhD student UTC

Research

I’m member of the theme “Plant Metabolism and Bioresources” and my current research consists in studying the connection between lipid metabolism and circadian clock in Arabidopsis thaliana (under the supervision of A. Troncoso-Ponce).

Thus, Arabidopsis thaliana is cultivated under different conditions of exposure to light (constant condition or 12h in light) to determine whether lipid metabolism only depend on the diurnal rhythm or circadian rhythm too.

In the same time, lipid constituents of different circadian clock mutants are compared to their wild-type strains to identify metabolic pathways that are connected to the circadian clock.
 

Skills

Scientific skills:

Lipidomics: Connection between lipid metabolism and circadian clock in Arabidopsis thaliana, analyze of mycobacterial lipids

Metabolomics: Role of microbiota against pathogenic bacteria, comparison of different types of milk using NMR-based metabolomics.

Laboratory skills:

NMR, Mass spectrometry, extraction of metabolites and lipids, statistics (multivariate and univariate), cell culture (prokaryotic and eukaryotic), seedling culture, other skills (SDS-PAGE, Western Blot, microscopy).
 

General information

Education

  • 2015-2017: Master’s degree in Metabolomics, Paul Sabatier University.
  • 2012-2015: Bachelor’s degree in Biochemistry

Profesionnal experiences:

  • 2015 (3 months): Engineer in Biochemistry (Lipidomics)
  • 2017 (6 months): Engineer in Biochemistry (Metabolomics)
  • 2017 (3 months): Engineer in Biochemistry (Metabolomics)
  • Since October 2018: PhD Student in Metabolomics/lipidomics. University of Compiègne, « Génie Enzymatique et Cellulaire ».

 
 

Personal interests

Music: Violin, Sport: Basketball

 

Paulina MEDINA RANGEL

PhD student UTC

Research

Member of the theme “Biomimicry and Biomolecular Diversity”.

PhD project: “Molecularly imprinted polymer nanocomposites as plastic antibodies for cell recognition” under the supervision of B. Tse-Sum-Bui and K. Haupt.

Synthesis of biomimetic polymers for biosensing, bioimaging and biomedical applications.

 

Skills

  • Scientific writing and oral scientific communication
  • Technical skills in cellular biology (cell culture of immortalized, cancer and primary cells), microscopy (light microscopy, epifluorescence and confocal), analytical methods (spectrophotometry, fluorometry, radioactivity, enzymatic assays), polymer chemistry, nanoparticles characterization
  • Languages: Spanish (native), English (fluent), French (fluent), Italian (basic)

 

Roles and responsabilities

Student representative council

 

General information

  • Bachelor degree in Biochemical Engineering, Autonomous University of Aguascalientes (Mexico)
  • Master degree in Biological Engineering, University of Technology of Compiègne (France)
  • PhD student in University of Technology of Compiègne (France)

 

Personal interests

Music, reading.

 

Main publications

Articles

Mier A., Nestora S., Medina Rangel P., Rossez Y., Haupt K., Tse Sum Bui B. Cytocompatibility of Moleculary Imprinted Polymers for Deodorants : Evaluation on Human Keratinocytes and Axillary-Hosted Bacteria . ACS Appl. Mater. Interfaces 2019, 2 ,3439-3447    ABSTRACT: https://doi.org/10.1021/acsabm.9b00388

Medina Rangel P., Caclef S., Xu J., Panagiotopoulou M., Kovensky J., Tse Sum Bui B., Haupt K. Solid-phase synthesis of moleculary imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans. Scientific Reports2019, 9,3923.
 

Medina Rangel P., Moroni E., Merlier F., Gheber L., Vago R., Tse Sum Bui B., Haupt K. Chemical Antibody Mimics Inhibit Cadherin-Mediated Cell-Cell Adhesion: A Promising Strategy for Cancer Therapy. Angewandte Chemie2019, In press ,.
 

Alejandra MIER

PhD student UTC

Research

Member of the theme “Biomimicry and Biomolecular Diversity”.

PhD project: “Molecularly imprinted polymer nanocomposites as antibody mimics for cancer cell recognition and theranostics” under the supervision of B. Tse-Sum-Bui and K. Haupt.
Skills

  • Technical skills in biology, cell engineering (cell culture, cytotoxicity assays, ELISA, etc), microscopy (epifluorescence), microfluidics, analytical methods, polymer chemistry and nanoparticles characterization.
  • Languages: Spanish (native), English (fluent), French (fluent)

 

Roles and responsabilities

Administrative management of the MIP group.
General information

  • Bachelor degree in Biochemical Engineering, Autonomous University of Aguascalientes (Mexico)
  • Master degree in Biological Engineering, University of Technology of Compiègne (France)
  • PhD student in University of Technology of Compiègne (France)

 

Personal interests 

Travel

 

Main publications

Articles

Mier A., Nestora S., Medina Rangel P., Rossez Y., Haupt K., Tse Sum Bui B. Cytocompatibility of Moleculary Imprinted Polymers for Deodorants : Evaluation on Human Keratinocytes and Axillary-Hosted Bacteria . ACS Appl. Mater. Interfaces 2019, 2 ,3439-3447    ABSTRACT: https://doi.org/10.1021/acsabm.9b00388

Ernesto PARULI III

PhD student

Research

Member of the theme “Biomimicry and Biomolecular Diversity”.

PhD project: “Direct writing of 3D MIP micro and nanosensors by two photon stereolithography” under the supervision of C. Gonzato and K. Haupt.

Novel click chemistry for the synthesis of molecularly imprinted polymers (MIPs); two-photon stereolithography for the direct writing of 3D molecularly imprinted polymers (MIPs)

 

Skills

Chemical sensors and biosensors; analytical chemistry; polymer chemistry; use of Nanoscribe Photonic Professional (GT) 3D printer; work in a radioactivity laboratory; UV-Vis; fluorescence; infrared spectroscopies

General information

  • Bachelor of Science in Chemistry, University of Santo Tomas (Philippines)
  • Bachelor thesis: “Chitosan/citric acid-based piezoelectric sensor for trimethylamine”
  • Master of Science in Chemistry, University of Santo Tomas (Philippines)
  • Master thesis: “Aptamer-coupled quartz crystal as histamine sensor”
  • PhD student, Université de Technologie de Compiègne (France)
  • PhD thesis: “Direct writing of 3D MIP micro and nanosensors by two photon stereolithography”

 

Personal interests

Music, reading, learning languages, badminton

Nely RODRIGUEZ

PhD student UPJV

Research

Member of the theme “Plant Metabolism and Bioresources”.

PhD project: Anti-fungal properties of natural amphiphiles as viewed by molecular dynamics simulations (under the supervision of S. Buchoux).
Skills

Biophysics, computing and molecular dynamics.
General information

  • Licence & M1: Physics, Murcia University (Spain).
  • M2: Biological systems and physical concepts, UPMC Paris (France).
  • PhD in biophysics:  UPJV, Amiens (France).

 

Personal interests

Sport, nature.

Ye TAO

PhD student UTC

Research

Member of the them “Biomimicry and Biomolecular Diversity”.

My PhD project of Y. Tao aims to develop a new strategy of biofilm degradation of P. aeruginosa bacteria by immobilizing a MIP targeting a DNase epitope under the supervision of L. Duma and Y. Rossez.
 

Skills

Molecular imprinting technology, HPLC, Cell culture.

Solid-phase synthesis of molecularly imprinted polymer nanoparticles for biofilm recognition.
 

General information

  • 09/2011-07/2015 : Ji Lin Business And Technology College Food Engineering; Major: Food Science and Engineering; Degree: Bachelor of Engineering
  • 09/2015-06/2018: Wuhan Polytechnic University; Major: Food Science and Engineering; Research direction: Grain, Oils and Vegetable Protein Engineering
  • 09/2018-present: Université de Technologie de Compiègne, UMR 7025 CNRS Enzyme and Cell Engineering

 

Personal interests

Basketball, swimming, music, skiing, sleeping