Bérangère BIHAN-AVALLE

PR UTC - Co-Animator Theme BBD

    Contact Bérangère Bihan-Avalle

 

Research

Member and head of the theme “Biomimicry and Biomolecular Diversity”.

Development of tool for molecular recognition with a close interest in the generation of diversity by elaborating libraries and selection procedures.

Know-how in the construction of libraries (oligonucleotide, peptide and protein libraries) and associated selection process (Phage Display and SELEX), in order to identify new binders towards various identified target.

Main inventor in a patent related to the use of aptamers in Leukemia (WO2015/140479)

 

 

 

Articles

Xu J., Merlier F., Avalle B., Veillard V., Debré P., Haupt K., Tse Sum Bui B. Molecularly Imprinted Polymer Nanoparticles as Potential Synthetic Antibodies for Immunoprotection against HIV . ACS Appl. Mater. Interfaces, 2019, 11,9824-9831.
     ABSTRACT: We describe the preparation and characterization of synthetic antibodies based on molecularly imprinted polymer nanoparticles (MIP-NPs) for the recognition and binding of the highly conserved and specific peptide motif SWSNKS (3S), an epitope of the envelope glycoprotein 41 (gp41) of human immunodeficiency virus type 1 (HIV-1). This motif is implicated in the decline of CD4+ T cells and leads to the deterioration of the immune system during HIV infection. Therefore, the development of MIP-NPs that can target and block the 3S peptide to prevent subsequent cascade interactions directed toward the killing of CD4+ T cells is of prime importance. Because most antibodies recognize their protein antigen via a conformational or structured epitope (as opposed to a linear epitope commonly used for molecular imprinting), we employed protein molecular modeling to design our template epitope so that it mimics the three-dimensional structure fold of 3S in gp41. The resulting template peptide corresponds to a cyclic structure composed of CGSWSNKSC, with the 3S motif well orientated for imprinting. MIP-NPs with a size of 65 nm were obtained by solid-phase synthesis and were water-soluble. They were prepared by a judicious combination of multiple functional monomers affording hydrogen bonding, ionic, π–π, and hydrophobic interactions, conferring high affinity and selectivity toward both the cyclic peptide and the whole gp41 protein. These results suggest that our MIPs could potentially be used for blocking the function of the 3S motif on the virus.

Shahsavarian M., Chaaya N., Costa N., Boquet D., Atkinson A., Offmann B., Kaveri Sv., Lacroix-desmazes S., Friboulet A., Avalle B. & Padiolleau-lefevre S. Multi-target selection of catalytic antibodies wih . FEBS J., 2017, 284,634-653.
     ABSTRACT: ??lactamase enzymes responsible for bacterial resistance to antibiotics are among the most important health threats to the human population today. Understanding the increasingly vast structural motifs responsible for the catalytic mechanism of ??lactamases will help improve the future design of new generation antibiotics and mechanism?based inhibitors of these enzymes. Here we report the construction of a large murine single chain fragment variable (scFv) phage display library of size 2.7 ? 109 with extended diversity by combining different mouse models. We have used two molecularly different inhibitors of the R?TEM ??lactamase as targets for selection of catalytic antibodies with ??lactamase activity. This novel methodology has led to the isolation of five antibody fragments, which are all capable of hydrolyzing the ??lactam ring. Structural modeling of the selected scFv has revealed the presence of different motifs in each of the antibody fragments potentially responsible for their catalytic activity. Our results confirm (a) the validity of using our two target inhibitors for the in vitro selection of catalytic antibodies endowed with ??lactamase activity, and (b) the plasticity of the ??lactamase active site responsible for the wide resistance of these enzymes to clinically available inhibitors and antibiotics.

Padiolleau-lefevre S., Ben Naya R., Shahsavarian M., Friboulet A. & Avalle B. Catalytic antibodies and their applications in biotechnology: State of the art.. Biotechnol. Lett., 2014, 36,1369-1379.
 

Shahsavarian M., Le Minoux D., Matti K. M., Kaveri S., Lacroix-desmazes S., Boquet D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Exploitation of rolling circle amplification for the construction of large phafe display antibody libraries.. J. Immunol. Methods, 2014, 407,26-34.
 

Ben Naya R., Matti K., Guellier A., Matagne A., Boquet D., Thomas D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Efficient refolding of a recombinant abzyme: Structural and catalytic characterizations.. Appl. Microbiol. Biotechnol., 2013, 97,7721-7731.
 

Rosant C., Avalle B., Larcher D., Dupont A., Friboulet A. & Tarascon J. M. Biosynthesis of electrode nanomaterials by phage engineering: comprehension and future.. Energ. Environ. Sci., 2012, 5,9936-9943.
 

Le Minoux D., Mahendra A., Lacroix-desmazes S., Limnios N., Friboulet A., Avalle B., Boquet D., Kaveri S. & Padiolleau-lefevre S. A novel molecular analysis of genes encoding catalytic antibodies.. Mol. Immunol., 2012, 50,160-168.
 

Gabibov A. G., Belogurov A. A., Lomakin Y. A., Zakharova M. Y., Avakyan M. E., Dubrovskaya V. V., Smirnov I. V., Ivanov A. S., Molnar A. A., Gurtsevitch V. E., Diduk S. V., Smirnova K. V., Avalle B., Sharanova S. N., Tramontano A., Friboulet A., Boyko A. N., Ponomarenko N. A. & Tikumova N. V. Combinatorial antibody library from Multiple Sclerosis patients reveals antibodies that cross-react with myelin basic protein and EBV-antigen.. FASEB J., 2011, 25,4211-4221.
 

Smirnov I., Carletti E., Kurkova I., Nachon F., Nicolet Y., Mitkevich V., Debat H., Avalle B., Belogurov A., Kuznetsov N., Reshetnyak A., Masson P., Tonevitsky A., Ponomarenko N., Makarov A. A., Friboulet A., Tramontano A. & Gabibov A. G. Reactibodies generated by kinetic selection couple chemical reactivity with faborable protein dynamics.. Proc. Natl. Acad. Sci. USA, 2011, 108,15954-1959.
 

Phichith D., Bun S., Padiolleau-lefevre S., Guellier A., Banh S., Gallieni M., Frere J. M., Thomas D., Friboulet A. & Avalle B. Novel peptide inhibiting both TEM-1 beta-lactamase and penicillin-binding proteins.. FEBS J., 2010, 277,4965-4972.
 

Phichith D., Bun S., Padiolleau-lefevre S., Banh S., Thomas D., Friboulet A. & Avalle B. Mutational and inhibitory analysis of a catalytic antibody. Implication for drug discovery.. Mol. Immunol., 2009, 47,348-356.
 

Smirnov I. V., Vorobiev I. I., Friboulet A., Avalle B., Thomas D., Knorre V. D., Gabibov A. G. & Ponomarenko N. A. The antiidiotypic approach to obtaining a proteolytic antibody.. Dokl. Biochem. Biophys., 2008, 420,105-107.
 

Belogurov A. A., Kurkova I. N., Friboulet A., Thomas D., Misikov V. K., Zakharova M. U., Suchkov S. V., Kotov V., Alexin A. I., Avalle B., Souslova E. A., Morse H. C., Gabibov A. G. & Ponomarenko N. A. Recognition and Degradation of Myelin Basic Protein Peptides by Serum Autoantibodies as a Novel Biomarker for Multiple Sclerosis.. J. Immunol., 2008, 180,1258-1267.
 

Ponomarenko N. A., Pillet D., Paon M., Vorobiev I. I., Smirnov I. V., Adenier H., Avalle B., Kolesnikov A. V., Kozyr A. V., Thomas D., Gabibov A. G. & Friboulet A. Anti-idiotypic antibody mimics proteolytic function of parent antigen.. Biochemistry , 2007, 46,14598-14609.
 

Padiolleau-lefevre S., Debat H., Phichith D., Thomas D., Friboulet A. & Avalle B. Expression of a functional scFv fragment of an anti-idiotypic antibody with a ß-lactam hydrolytic activity. . Immunol. Letters, 2006, 103,39-44.
 

Ponomarenko N. A., Vorobiev I. I., Alexandrova E. S., Reshetnyak A. V., Telegin G. B., Khaidurov S. V., Avalle B., Karavanov A., Morse H. C., Thomas D., Friboulet A. & Gabibov A. G. Induction of protein-targeted catalytic response in autoimmune prone mice: antibody-mediated cleavage of HIV-1 glycoprotein gp120. . Biochemistry, 2006, 45,324-330.
 

Ponomarenko N. A., Durova O. M., Vorobiev I. I., Belogurov A. A., Telegin G. B., Suchkov S. V., Misikov V. K., Kiselev S. L., Lagarkova M. A., Govorun V. M., Serebryakova M. V., Avalle B., Tornatore P., Karavanov A., Morse H. C., Thomas D., Friboulet A. & Gabibov A. G. Autoantibodies to myelin basic protein catalyze site specific degradation of their antigen. . Proc. Natl. Acad. Sci. USA, 2006, 103,281-286.
 

Chaaya N., Shahsavarian M., Maffucci I., Friboulet A., Offmann B., Leger J. B., Rousseau S., Avalle B., Padiolleau-lefevre S. Genetic background and immunological status influence B cell repertoire diversity in mice . Scientific Reports , 0, 9,14261 .
 

Patent

B. Avalle-bihan, A. Friboulet, H. Isber, C. Loussouarn, S. Padiolleau-lefèvre. Inhibiteurs de STAT5 et utilisation de ceux-ci. , 2014, ,.
 

A. Friboulet, B. Avalle-bihan, H. Débat, D. Thomas Composés capables de moduler l'activité et de stimuler la production d'un anticorps catalytique. France, n° 0106754, 2001, ,.
 

Karsten HAUPT

PR UTC - Director

        Contact Karsten Haupt

Articles

Blersch J., Francisco V., Rebelo C., Jimérez-balsa A., Antunes H., Gonzaro C., Pinto S., Liedl K., Haupt K., Ferreira L. A Light‐Triggerable Nanoparticle Library for the Controlled Release of Non‐Coding RNAs . Angewandte Chemie , 2020, 59 ,1985-1991 .
 

Leibl N., Duma L., Gonzato C. Et Haupt K. Polydopamine-based molecularly imprinted thin films for electro-chemical sensing of nitro-explosives in aqueous solutions . Bioelectrochemistry , 2020, 135 ,107541 .
 

Montagna V., Haupt L., Gonzalo C. RAFT coupling chemistry: A general approach for post-functionalizing molecularly imprinted polymers synthesized by radical polymerization . Polymer Chem. , 2020, 11,1055-1061 .
 

Medina Rangel P., Moroni E., Merlier F., Gheber L., Vago R., Tse Sum Bui B., Haupt K. Chemical Antibody Mimics Inhibit Cadherin-Mediated Cell-Cell Adhesion: A Promising Strategy for Cancer Therapy. Angewandte Chemie, 2019, In press ,.
 

Medina Rangel P., Caclef S., Xu J., Panagiotopoulou M., Kovensky J., Tse Sum Bui B., Haupt K. Solid-phase synthesis of moleculary imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans. Scientific Reports, 2019, 9,3923.
 

Xu J., Merlier F., Avalle B., Veillard V., Debré P., Haupt K., Tse Sum Bui B. Molecularly Imprinted Polymer Nanoparticles as Potential Synthetic Antibodies for Immunoprotection against HIV . ACS Appl. Mater. Interfaces, 2019, 11,9824-9831.
     ABSTRACT: We describe the preparation and characterization of synthetic antibodies based on molecularly imprinted polymer nanoparticles (MIP-NPs) for the recognition and binding of the highly conserved and specific peptide motif SWSNKS (3S), an epitope of the envelope glycoprotein 41 (gp41) of human immunodeficiency virus type 1 (HIV-1). This motif is implicated in the decline of CD4+ T cells and leads to the deterioration of the immune system during HIV infection. Therefore, the development of MIP-NPs that can target and block the 3S peptide to prevent subsequent cascade interactions directed toward the killing of CD4+ T cells is of prime importance. Because most antibodies recognize their protein antigen via a conformational or structured epitope (as opposed to a linear epitope commonly used for molecular imprinting), we employed protein molecular modeling to design our template epitope so that it mimics the three-dimensional structure fold of 3S in gp41. The resulting template peptide corresponds to a cyclic structure composed of CGSWSNKSC, with the 3S motif well orientated for imprinting. MIP-NPs with a size of 65 nm were obtained by solid-phase synthesis and were water-soluble. They were prepared by a judicious combination of multiple functional monomers affording hydrogen bonding, ionic, π–π, and hydrophobic interactions, conferring high affinity and selectivity toward both the cyclic peptide and the whole gp41 protein. These results suggest that our MIPs could potentially be used for blocking the function of the 3S motif on the virus.

Paruli E Iii., Griesser T., Merlier F., Gonzaro C., Haupt K. Molecularly imprinted polymers by thiol–yne chemistry: making imprinting even easier. Polymer Chem. , 2019, 10,4732-4739.
 

Mier A., Nestora S., Medina Rangel P., Rossez Y., Haupt K., Tse Sum Bui B. Cytocompatibility of Moleculary Imprinted Polymers for Deodorants : Evaluation on Human Keratinocytes and Axillary-Hosted Bacteria . ACS Appl. Mater. Interfaces , 2019, 2 ,3439-3447    ABSTRACT: https://doi.org/10.1021/acsabm.9b00388

Xu J., Prost E., Haupt K. & Tse Sum Bui B. Direct and sensitive determination of trypsin in human urine using a water-soluble signaling fluorescent molecularly imprinted polymer nanoprobe. . Sensor. Actuat. B-Chem. , 2018, 258,10-17.
     ABSTRACT: https://doi.org/10.1016/j.snb.2017.11.077

Mattsson L., Xu J., Preininger C., Tse Sum Bui B. & Haupt K. Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix.. Talanta, 2018, 181,190-196.
     ABSTRACT: We developed a competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines in fish samples. MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a complex sample matrix, such as fish extract, into the assay changes the environment and the binding conditions, therefore the importance of the sample preparation is extensively discussed. Several extraction and purification methods for fish were comprehensively studied, and an optimal clean-up procedure for fish samples using liquid-liquid extraction was developed. The feasibility of the competitive MIP assay was shown in the purified fish extract over a broad histamine range (1 ? 430ʵM). The MIP had the highest affinity towards histamine, but recognized also the structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine, providing simultaneous analysis and assessment of the total amount of biogenic amines.

Demir B., Lemberger M. M., Panagiotopoulou M., Medina Rangel P. X., Timur S., Hirsch T., Tse Sum Bui B., Wegener J. & Haupt K. Tracking hyaluronan: molecularly imprinted polymer coated carbon dots for cancer cell targeting and imaging.. ACS Appl. Mater. Interfaces, 2018, 10,3305-3313.
     ABSTRACT: War against cancer constantly requires new affinity tools to selectively detect, localize, and quantify biomarkers for diagnosis or prognosis. Herein, carbon nanodots (CDs), an emerging class of fluorescent nanomaterials, coupled with molecularly imprinted polymers (MIPs), are employed as a biocompatible optical imaging tool for probing cancer biomarkers. First, N-doped CDs were prepared by hydrothermal synthesis using starch as carbon source and l-tryptophan as nitrogen atom provider to achieve a high quantum yield of 25.1

Mourao C. A., Bokeloh F., Xu J., Prost E., Duma L., Merlier F., Bueno S. M. A., Haupt K. & Tse Sum Bui, B. Dual oriented solid phase molecular imprinting: Toward selective artificial receptors for recognition of nucleotides in water. Macromolecules, 2017, 50,7484-7490.
     ABSTRACT: We describe the synthesis of water-soluble molecularly imprinted polymer nanoparticles (MIP-NPs) as a new artificial host receptor for the recognition of adenosine monophosphate (AMP), used herein, as a model nucleotide. MIP-NPs were prepared by solid-phase synthesis on glass beads (GB) using, for the first time, immobilized Fe(III)-chelate as an affinity ligand to orientate the AMP via its phosphate group. A polymerizable thymine monomer which can induce complementary base-pairing with the adenine moiety of the nucleotide was synthesized and incorporated in the polymerization mixture to constrain the AMP in a dual-orientated configuration. The MIP-NPs were remarkably selective toward AMP as they did not bind other nucleotides, GMP, UMP, and CMP. This strategy of using the phosphate group of AMP as a hinge enables unhindered pairing of the nucleobase with its corresponding complementary base monomer and can be extended to the preparation of specific MIP receptors for other key nucleotides in aqueous conditions.

Panagiotopoulou M., Kunath S., Medina-rangel P. X., Haupt K. & Tse Sum Bui B. Fluorescent molecularly imprinted polymers as plastic antibodies for selective labeling and imaging of hyaluronan and sialic acid on fixed and living cells.. Biosens. Bioelectron., 2017, 88,85-93.
     ABSTRACT: Altered glycosylation levels or distribution of sialic acids (SA) or hyaluronan in animal cells are indicators of pathological conditions like infection or malignancy. We applied fluorescently-labeled molecularly imprinted polymer (MIP) particles for bioimaging of fixed and living human keratinocytes, to localize hyaluronan and sialylation sites. MIPs were prepared with the templates D-glucuronic acid (GlcA), a substructure of hyaluronan, and N-acetylneuraminic acid (NANA), the most common member of SA. Both MIPs were found to be highly selective towards their target monosaccharides, as no cross-reactivity was observed with other sugars like N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, D-glucose and D-galactose, present on the cell surface. The dye rhodamine and two InP/ZnS quantum dots (QDs) emitting in the green and in the red regions were used as fluorescent probes. Rhodamine-MIPGlcA and rhodamine-MIPNANA were synthesized as monodispersed 400 nm sized particles and were found to bind selectively their targets located in the extracellular region, as imaged by epifluorescence and confocal microscopy. In contrast, when MIP-GlcA and MIP-NANA particles with a smaller size (125 nm) were used, the MIPs being synthesized as thin shells around green and red emitting QDs respectively, it was possible to stain the intracellular and pericellular regions as well. In addition, simultaneous dual-color imaging with the two different colored QDs-MIPs was demonstrated. Importantly, the MIPs were not cytotoxic and did not affect cell viability; neither was the cells morphology affected as demonstrated by live cell imaging. These synthetic receptors could offer a new and promising imaging tool to monitor disease progression.

Karczmarczyk, A., Haupt K. & Feller K. H. Development of a QCM-D biosensor for Ochratoxin A detection in red wine. Talanta, 2017, 166,193-197.
     ABSTRACT: Ochratoxin A (OTA), a highly toxic compound, is one of the most widely spread mycotoxins that contaminates a large variety of agricultural commodities. Due to its presence in the food chain, it imposes a hazard on both human and animal health. Therefore, there is a need for precise, fast and simple methods for toxin quantification. Herein, a novel sensor based on a quartz crystal microbalance with dissipation monitoring (QCM-D) and antibodies for specific analyte recognition was developed for rapid and sensitive detection of OTA in red wine. The combination of indirect competitive assay with QCM-D gives a straightforward device, which can simultaneously measure frequency (?f) and dissipation (?D) changes resulting in detailed information about the mass attached to the sensor surface as well as conformational changes, viscoelastic properties and the hydration state of the film. Small molecules (such as OTA) suffer from poor LOD due to the high concentration of primary antibody needed to generate adequate signal. In the present study, amplification of the QCM-D signal was obtained by applying secondary antibodies conjugated with gold nanoparticles (AuNPs). Thanks to this, a linear detection range of 0.2

Attieh M. D., Zhao Y., Elkak A., Falcimaigne-cordin A., & Haupt K. Enzyme-initiated free-radical polymerization of molecularly imprinted polymer nanogels on a solid phase with an immobilized radical source. Angew. Chem. Int. Ed., 2017, 56,3339-3343.
     ABSTRACT: An enzyme?mediated synthetic approach is described for the preparation of molecularly imprinted polymer nanoparticles (MIP?NPs) in aqueous media. Horseradish peroxidase (HRP) was used to initiate the polymerization of methacrylate or vinyl monomers and cross?linkers by catalyzing the generation of free radicals. To prevent entrapment of the enzyme in the cross?linked polymer, and to enable it to be reused, HRP was immobilized on a solid support. MIPs based on 4?vinylpyridine and 1,4?bis(acryloyl)piperazine for the recognition of 2,4?dichlorophenoxyacetic acid (2,4?D) and salicylic acid were synthesized in an aqueous medium. MIPs for the protein trypsin were also synthesized. MIP nanoparticles with sizes between 50 and 300?nm were obtained with good binding properties, a good imprinting effect, and high selectivity for the target molecule. The reusability of immobilized HRP for MIP synthesis was shown for several batches.

Maes T., Jessop R., Wellner N., Haupt K. & Mayes A. G. A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red. Sci. Rep., 2017, 7,44501.
     ABSTRACT: A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Garcia-soto M., Haupt K., Gonzato C. Synthesis of molecularly imprinted polymers by photo-iniferter polymerization under visible light.. Polym. Chem., 2017, 8,4830-4834.
     ABSTRACT: The easiest way to make polymers able to specifically recognize a target molecule consists of copolymerizing functional monomers and a cross-linker around a molecular template. This method affords a rigid material bearing high affinity binding sites, namely a synthetic receptor or antibody mimic, known as the

Yarman A., Jetzschmann K. J., Neumann B., Zhang X., Wollenberger U., Cordin A., Haupt K. & Scheller F. W. Enzymes as tools in MIP-sensors.. Chemosensors, 2017, 5,11.
     ABSTRACT: Molecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and are potentially cheaper to produce. However, the affinity and especially the selectivity of MIPs are in general lower than those of their biological pendants. Enzymes are useful tools for the preparation of MIPs for both low and high-molecular weight targets: As a green alternative to the well-established methods of chemical polymerization, enzyme-initiated polymerization has been introduced and the removal of protein templates by proteases has been successfully applied. Furthermore, MIPs have been coupled with enzymes in order to enhance the analytical performance of biomimetic sensors: Enzymes have been used in MIP-sensors as

Xu J, Haupt K. & Tse Sum Bui B. Core-shell molecularly imprinted polymer nanoparticles as synthetic antibodies in a sandwich fluoroimmunoassay for trypsin determination in human serum.. ACS Appl. Mater. Interfaces, 2017, 9, 24476?24483.
     ABSTRACT: We describe the application of a fluorescently labeled water-soluble core

Panagiotopoulou M., Salinas Y., Beyazit S., Kunath S., Mayes A. G., Duma L., Prost E., Resmini M., Tse Sum Bui B. & Haupt K. Molecularly imprinted polymer-coated quantum dots for multiplexed cell targeting and imaging.. Angew. Chem. Int. Ed., 2016, 55,8244-8248.
 

Beyazit S., Tse Sum Bui B., Haupt K. & Gonzato C. Molecularly imprinted polymer nanomaterials and nanocomposites by controlled/living radical polymerization.. Prog. Polym. Sci., 2016, 62,1-21.
 

Parlak O., Beyazit S., Tse Sum Bui B., Haupt K., Turner A. P. F. & Tiwari A. Programmable bioelectronics in a stimuli-encoded 3D graphene interface.. Nanoscale, 2016, 8,9976-9981.
 

Bonomi P., Attieh M. D., Gonzato C. & Haupt K. A novel versatile water soluble iniferter platform for the preparation of imprinted nanoparticles via photopolymerization in aqueous media.. Chem.-Eur. J., 2016, 22,10150-10154.
 

Xu J. J., Ambrosini S., Tamahkar E., Rossi C., Haupt K. & Tse Sum Bui B. Toward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins.. Biomacromolecules, 2016, 17,345-353.
 

Nestora S., Merlier F., Beyazit S., Prost E., Duma L., Baril B., Graves A., Haupt K. & Tse Sum Bui B. Plastic antibodies for cosmetics: Molecularly imprinted polymers scavenge precursors of malodors. . Angew. Chem. Int. Ed., 2016, 55,6252-6256.
 

Nestora S., Merlier F., Prost E., Haupt K., Rossi C. & Tse Sum Bui B. Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer.. J. Chromatogr. A, 2016, 1465,47-54.
 

Canfarotta F., Waters A., Sadler R., Mcgill P., Guerreiro A., Papkovsky D., Haupt K. & Piletsky S. Biocompatibility and internalization of moleculary imprinted nanoparticles.. Nano Res., 2016, 9,3463-3477.
 

Chia Gomez L. P., Spangenberg A., Ton X. A., Fuchs Y., Bokeloh F., Malval J. P., Tse Sum Bui B., Thuau D., Ayela C., Haupt K. & Soppera O. Rapid prototyping of chemical microsensors based on molecularly imprinted polymers synthesized by two-photon stereolithography.. Adv. Mater., 2016, 28,5931-5937.
 

Parlak O., Beyazit S., Jafari M. J., Tse Sum Bui B., Haupt K., Tiwari A. & Turner A. P. F. Light-triggered switchable graphene-polymer hybrid bioelectronics.. Adv. Mater. Interfaces, 2016, 3,1500353.
 

Kunath S., Panagiotopoulou M., Maximilien J., Marchyk N., Sanger J. & Haupt K. Cell and tissue imaging with molecularly imprinted polymers as plastic antibody mimics.. Adv. Healthc. Mater., 2015, 4,1322-1326.
 

Adali-kaya Z., Tse Sum Bui B., Falcimaigne-cordin A. & Haupt K. Molecularly imprinted polymer nanomaterials and nanocomposites: atom-transfer radical polymerization with acidic monomers.. Angew. Chem. Int. Ed., 2015, 54,192-195.
 

Starok M., Preira P., Vayssade M., Haupt K., Salome L. & Rossi C. EGFR inhibition by curcumin in cancer cells: a dual mode of action.. Biomacromolecules, 2015, 16,1634-1642.
 

Ton X. A., Acha V., Bonomi P., Tse Sum Bui B. & Haupt K. A disposable evanescent wave fiber optic sensor coated with a molecularly imprinted polymer as a selective fluorescent probe.. Biosens. Bioelectron., 2015, 64,359-366.
 

Panagiotopoulou M., Beyazit S., Nestora S., Haupt K., & Tse Sum Bui B. Initiator-free synthesis of molecularly imprinted polymers by polymerization of self-initiated monomers.. Polymer, 2015, 66,43-51.
 

Bompart M., Goto A., Wattraint O., Sarazin C., Tsujii Y., Gonzato C. & Haupt K. Molecularly imprinted polymers by reversible chain transfer catalyzed polymerization.. Polymer, 2015, 78,31-36.
 

Foguel M. V., Ton X. A., Zanoni M. V. B., Sotomayor M. D. P. T., Haupt K. & Tse Sum Bui B. A molecularly imprinted polymer-based evanescent wave fiber optic sensor for the detection of basic red 9 dye. . Sens. Actuators B, 2015, 218,222-228.
 

Fuchs Y., Kunath S., Soppera O., Haupt K. & Mayes A. G. Molecularly imprinted silver-halide reflection holograms for label-free opto-chemical sensing.. Adv. Funct. Mater., 2014, 24,688-694.
 

Ayela C., Dubourg G., Pellet C. & Haupt K. All-organic micromechanical systems integrating specific molecular recognition. A new generation of chemical sensors.. Adv. Mater., 2014, 26,5876-5879.
 

Beyazit S., Ambrosini S., Marchyk N., Palo E., Kale V., Soukka T., Tse Sum Bui B. & Haupt K. Versatile synthetic strategy for coating upconverting nanoparticles with polymer shells through localized photopolymerization by using the particles as internal light sources.. Angew. Chem. Int. Ed., 2014, 53,8919-8923.
 

Li B., Xu J., Hall A. J., Haupt K. & Tse Sum Bui B. Water-compatible silica sol-gel molecularly imprinted polymer as potential delivery system for the controlled release of salicylic acid.. J. Mol. Recognit., 2014, 27,559-565.
 

Marchyk N., Maximilien J., Beyazit S., Haupt K. & Tse Sum Bui B. One-pot synthesis of iniferter-bound polystyrene core nanoparticles for the controlled grafting of multilayer shells.. Nanoscale, 2014, 6,2872-2878.
 

Gonzato C., Pasetto P., Bedoui F., Mazeran P. E. & Haupt K. On the effect of using RAFT and FRP for the bulk synthesis of acrylic and methacrylic molecularly imprinted polymers.. Polymer Chem., 2014, 5,1313-1322.
 

Dmitrienko E. V., Bulushev R. D., Haupt K., Kosolobov S. S., Latyshev A. V., Pyshnaya I. A. & Pyshnyi D. V. A simple approach to prepare molecularly imprinted polymers from nylon-6.. J. Mol. Recognit., 2013, 26,368-375.
 

Kantarovich K., Tsarfati-barad I., Gheber L. A., Haupt K. & Bar I. Reading biochips by raman and surface-enhanced raman spectroscopies.. Plasmonics, 2013, 8,3-12.
 

Kunath S., Marchyk N., Haupt K. & Feller K. H. Multi-objective optimization and design of experiments as tools to tailor molecularly imprinted polymers specific for glucuronic acid.. Talanta, 2013, 105,211-218.
 

Ambrosini S., Beyazit S., Haupt K. & Tse Sum Bui B. Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition.. Chem. Commun., 2013, 49,6746-6748.
 

Ton X. A., Tse Sum Bui B., Resmini M., Bonomi P., Dika I., Soppera O. & Haupt K. A versatile fiber-optic fluorescence sensor based on molecularly imprinted microstructures polymerized in situ.. Angew. Chem. Int. Ed., 2013, 52,8317-8321.
 

Fuchs Y., Soppera O., Mayes A. G. & Haupt K. Holographic molecularly imprinted polymers for label-free chemical sensing.. Adv. Mater., 2013, 25,566-570.
 

Zdunek J., Benito-pena E., Linares A., Falcimaigne-cordin A., Orellana G., Haupt K. & Moreno-bondi M. C. Surface-imprinted nanofilaments for Europium-amplified luminescent detection of fluoroquinolones antibiotics.. Chem.-Eur. J., 2013, 19,10209-10216.
 

Cakir P., Cutivet A., Resmini M., Tse Sum Bui B. & Haupt K. Protein-size molecularly imprinted polymer nanogels as synthetic antibodies, by localized polymerization with multi-initiators.. Adv. Mater., 2013, 25,1048-1051.
 

Fuchs Y., Soppera O. & Haupt K. Photopolymerization and photostructuring of molecularly imprinted polymers for sensor applications-A review.. Anal. Chim. Acta, 2012, 717,7-20.
 

Ton X. A., Acha V., Haupt K. & Tse Sum Bui B. Direct fluorimetric sensing of UV-excited analytes in biological and environmental samples using molecularly imprinted polymer nanoparticles and fluorescence polarization.. Biosens. Bioelectron., 2012, 36,22-28.
 

Doumiati S., Haupt K. & Rossi C. Autophosphorylation activation and inhibition by curcumin of the epidermal growth factor receptor reconstituted in liposomes.. J. Mol. Recognit., 2012, 25,623-629.
 

Bompart M., Haupt K. & Ayela C. Micro and nanofabrication of molecularly imprinted polymers.. Top. Curr. Chem., 2012, 325,83-110.
 

Haupt K., Linares A. V., Bompart M. & Tse Sum Bui B. Molecularly imprinted polymers.. Top. Curr. Chem., 2012, 325,1-28.
 

Piperno S., Tse Sum Bui B., Haupt K. & Ghebert L. A. Immobilization of molecularly imprinted polymer nanoparticles in electrospun poly(vinyl alcohol) nanofibers.. Langmuir, 2011, 27,1547-1550.
 

Linares A. V., Falcimaigne-cordin A., Gheber L. A. & Haupt K. Patterning nanostructured, synthetic, polymeric receptors by simultaneous projection photolithography, nanomolding, and molecular imprinting.. Small, 2011, 7,2318-2325.
 

Diot J. D., Moreno I. G., Twigg G., Ortiz Mellet C., Haupt K., Butters T. D., Kovensky J. & Gouin S. G. Amphiphilic 1-deoxynojirimycin derivatives through click strategies for chemical chaperoning in N370S Gaucher cells.. J. Org. Chem., 2011, 76,7757-7768.
 

Harz S., Shimmelpfennig M., Tse Sum Bui B., Marchyk N., Haupt K. & Feller K. H. Fluorescence optical spectrally resolved sensor based on molecularly imprinted polymers and microfluidics.. Eng. Life Sci., 2011, 11,559-565.
 

Gonzato C., Courty M., Pasetto P. & Haupt K. Magnetic molecularly imprinted polymer nanocomposites via surface-initiated RAFT polymerization.. Adv. Funct. Mater., 2011, 21,3947-3953.
 

Servant A., Haupt K. & Resmini M. Tuning molecular recognition in water-soluble nanogels with enzyme-like activity for Kemp elimination.. Chem.-Eur. J., 2011, 17,11052-11059.
 

Fuchs Y., Linares A. V., Mayes A. G., Haupt K. & Soppera O. Ultrathin selective molecularly imprinted polymer microdots obtained by evanescent wave photopolymerization.. Chem. Mater., 2011, 23,3645-3651.
 

Tse Sum Bui B. & Haupt K. Preparation and evaluation of a molecularly imprinted polymer for the selective recognition of testosterone – application to molecularly imprinted sorbent assays.. J. Mol. Recognit., 2011, 24,1123-1129.
 

Lalo H., Ayela C., Dague E., Vieu C. & Haupt K. Nanopatterning molecularly imprinted polymers by soft lithography: a hierarchical approach.. Lab Chip, 2010, 10,1316-1318.
 

Claude B., Viron-lamy C., Haupt K. & Morin P. Synthesis of a molecularly imprinted polymer for the solid-phase extraction of betulin and betulinic acid from plane bark.. Phytochem. Anal., 2010, 21,180-185.
 

Haupt K. Plastic antibodies.. Nature Mater., 2010, 9,612-614.
 

Kantarovich K., Tsarfati I., Gheber L. A., Haupt K. & Bar I. Reading microdots of a molecularly imprinted polymer by surface-enhanced Raman spectroscopy.. Biosens. Bioelectron., 2010, 26,809-814.
 

Tse Sum Bui B., Merlier F. & Haupt K. Towards the use of a molecularly imprinted polymer in doping analysis :selective preconcentration and analysis of testosterone and epitestosterone in human urine.. Anal. Chem., 2010, 82,4420-4427.
 

Tse Sum Bui B. & Haupt K. Molecularly imprinted polymers : synthetic receptors in bioanalysis. Anal. Bioanal. Chem., 2010, 398,2481-2492.
 

Bompart M., De Wilde Y. & Haupt K. Chemical nanosensors based on composite molecularly imprinted polymer particles and surface-enhanced Raman scattering.. Adv. Mater., 2010, 22,2343-2348.
 

Diot J., Garcia-moreno M. I., Gouin S. G., Mellet C. O., Haupt K. & Kovensky J. Multivalent iminosugars to modulate affinity and selectivity for glycosidases.. Org. Biomol. Chem., 2009, 7,357-363.
 

Kantarovich K., Belmont A. S., Haupt K., Bar I. & Gheber L. A. Detection of template binding to molecularly imprinted polymers by Raman spectroscopy.. Appl. Phys. Lett., 2009, 94,194103.
 

Guillon S., Lemaire R., Linares A. V., Haupt K. & Ayela C. Single step patterning of molecularly imprinted polymers for large scale fabrication of microchips.. Lab Chip, 2009, 9,2987-2991.
 

Mhaka B., Cukrowska E., Tse Sum Bui B., Ramstrom O., Haupt K., Tutu H. & Chimuka L. Selective extraction of triazine herbicide from food samples based on a combination of a liquid membrane and molecularly imprinted polymers.. J. Chromatogr. A, 2009, 1216,6796-6801.
 

Cutivet A., Schembri C., Kovensky J. & Haupt K. Molecularly imprinted microgels as enzyme inhibitors.. J. Am. Chem. Soc., 2009, 131,14699-14702.
 

Bompart M., Geher L. A., De Wilde Y. & Haupt K. Direct detection of analyte binding to single molecularly imprinted polymer particles by confocal Raman spectroscopy.. Biosens. Bioelectron., 2009, 25,568-571.
 

Bompart M. & Haupt K. Molecularly imprinted polymers and controlled/living radical polymerization.. Aust. J. Chem., 2009, 62,751-761.
 

Kantarovich K., Tsarfati I., Gheber L. A., Haupt K. & Bar I. Writing droplets of molecularly imprinted polymers by nano fountain pen and detecting their molecular interactions by surface-enhanced Raman scattering.. Anal. Chem., 2009, 81,5686-5690.
 

Linares A. V., Vandevelde F., Pantigny J., Falcimaigne-cordin A. & Haupt K. Polymer films composed of surface-bound nanofilaments with a high aspect ratio, molecularly imprinted with small molecules and proteins.. Adv. Funct. Mater., 2009, 19,1299-1303.
 

El Kirat K., Bartkowski M. & Haupt K. Probing the recognition specificity of a protein molecularly imprinted polymer using force spectroscopy.. Biosens. Bioelectron., 2009, 24,2618-2624.
 

Tse Sum Bui B., Belmont A. S., Witters H. & Haupt K. Molecular recognition of endocrine disruptors by synthetic and natural 17beta-estradiol receptors: a comparative study.. Anal. Bioanal. Chem., 2008, 390,2081-2088.
 

Vandevelde F., Pantigny J., Pezron I., Gheber L. & Haupt K. Nanostructured molecularly imprinted polymer films as synthetic recognition layers.. Int. J. Nanotechnology, 2008, 5,757-768.
 

Claude B., Morin P., Lafosse M., Belmont A. S. & Haupt K. Selective solid-phase extraction of a triterpene acid from a plant extract by molecularly imprinted polymer.. Talanta, 2008, 75,344-350.
 

Belmont A. S., Jaeger S., Knopp D., Niessner R., Gauglitz G. & Haupt K. Molecularly imprinted polymer films for reflectometric interference spectroscopic sensors.. Biosens. Bioelectron., 2007, 22,3267-3272.
 

Sineriz F., Ikeda Y., Petit E., Bultel L., Haupt K., Kovensky J. & Papy-garcia D. Toward an alternative for specific recognition of sulfated sugars. Preparation of highly specific molecular imprinted polymers.. Tetrahedron, 2007, 63,1857-1862.
 

Vandevelde F., Leichle T., Ayela C., Bergaud C., Nicu L. & Haupt K. Direct patterning of molecularly imprinted microdot arrays for sensors and biochips.. Langmuir, 2007, 23,6490-6493.
 

Belmont A. S., Sokuler M., Haupt K. & Gheber L. A. Direct writing of molecularly imprinted microstructures using a nanofountain pen.. Appl. Phys. Lett., 2007, 90,193101.
 

Ayela C., Vandevelde F., Lagranfe D., Haupt K. & Nicu L. Combining resonant piezoelectric micromembranes with molecularly imprinted polymers.. Angew. Chem. Int. Ed., 2007, 46,9271-9274.
 

Rossi C. & Haupt K Application of the Doehlert experimental design to molecularly imprinted polymers: surface response optimization of specific template recognition as a function of the type and degree of cross-linking.. Anal. Bioanal. Chem., 2007, 389,455-460.
 

Vandevelde F., Belmont A. S., Pantigny J. & Haupt K. Hierarchically nanostructured polymer films based on molecularly imprinted surface-bound nanofilaments.. Adv. Mater., 2007, 19,3717-3720.
 

Grafe A., Haupt K. & Mohr G. J. Optical sensor materials for the detection of amines in organic solvent. . Anal. Chim. Acta, 2006, 565,42-47.
 

Hunt C. E., Pasetto P., Ansell R. J. & Haupt K. A fluorescence polarisation molecular imprint sorbent assay for 2,4-D : a non separation pseudo immunoassay. . Chem. Commun., 2006, 16,1754-1756.
 

Pichon V. & Haupt K. Affinity separations on molecularly imprinted polymers with special emphasis on solid-phase extraction. . J. Liquid Chromatogr., 2006, 29,989-1023.
 

BOOK

Bokeloh F., Ayela C. & Haupt K. Micro and nanofabrication of molecularly imprinted polymers . Molecularly Imprinted Polymers for Analytical Chemistry Applications , 2018, W. Kutner and P. Sindhu Sharma,pp. 167-197
  

Panagiotopoulou M., Kunath S., Haupt K., Tse Sum Bui B. Cell and Tissue Imaging with Molecularly Imprinted Polymers . Methods in Molecular Biology, 2017, ,PP.399-415
  

Xu J, Medina-rangel P. X., Haupt K. & Tse Sum Bui B. Guide to the preparation of molecularly imprinted polymer nanoparticles for protein recognition, by solid-phase synthesis. Methods Enzymol, 2017, ,pp.115-141
  

Maximilien J., Beyazit S., Rossi C., Haupt K. & Tse Sum Bui B Nanoparticles in biomedical applications. Measuring Biological impacts of nanomaterials, 2016, Springer International Publishing, Switzerland,pp. 177-210
  

Noel Angelo KALACAS

PhD student UTC
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Mickaël GUERIN

PhD student UTC

Research

 

Skills

General information

Personal interests

Christos GALANOS

PhD student UTC
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Kevin DE CASTRO COGLE

PhD student UTC
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Tiffany AUROY

PhD student UTC

Research

Member of the theme « Biomimicry and Biomolecular Diversity »

Synthesis of nanoparticles to target gram negative bacterias and drug release.

Subject: « Molecular imprinted polymers for the vectorization and the stabilization of antimicrobial peptides : new approaches to combat antibiotic resistance » under the supervision of K. Haupt and B. Bihan-Avalle

Skills

Organic synthesis

Molecular purification and analysis : GC, RMN, liquid chromatography

Production of proteins (PCR,cell culture, electrophoresis, purification) by recombinant plasmid,.

Biocatalysis (enzypme activity assays, reactions of bioconversion)

General information

Bachelor licence degree in Biochemical and Biopharmaceutical Chemistry, Aix-Marseille University (France)

Master degree in Chemistry speciality « Chemistry of Life », Aix-Marseille University (France)
Academic research internship (Organic synthesis, Biocatalysis), Molecular Sciences Institute of Marseille (France)

PhD in Biology, Biochemistry and Cell Engineering, University of Picardie Jules Verne and University of Technology of Compiègne (France)

Personal interests

Sport, Sophrology, Music

Claudia HERRERA LEON

Post-Doc UTC
Research

I’m working at the interface between the two themes and working under the supervision of Pr N. d’Amelio.

Main research topics:

  • Design and physicochemical characterization of antimicrobial peptides: NMR study of their interaction with the biomimetic membranes of cancer cells by NMR.
  • PhD project: Design and testing of selectivity and haemolytic properties of novel antimicrobial peptides based on sequence alignments (under the supervision of Pr N. d’Amelio).

 

Skills

Biochemistry, microbiology, molecular biology.

 

General information

  • Licence in pharmaceutical chemistry and biology, Veracruzana university (Mexico)
  • Master in Biotechnology, Meritorious Autonomous University of Puebla (Mexico)
  • PhD, Université de Picardie Jules Verne (Amiens, France)

 

Personal interests

Music, stand up paddle

Nicola D’AMELIO

PR UPJV - Co-animator theme BBD

        Contact Nicola D’Amelio

 

 

Research

I’m working at the interface between the theme “Plant Metabolism and Bioresources” and “Biomimicry and Biomolecular Recognition”.

Main research topics:

  • Structure and dynamics of biomolecules involved in cellular processes or pathologies: Antimicrobial and anticancer peptides interacting with membrane models, antibiotic effects on bacterial protein expression, proteins involved in cancer (kinases, matrix metalloprotinases)  and neurodegenerative disorders (a-synuclein, superoxide dismutase, WW domains), photosynthesis inhibition, and oxidative stress caused by metal ions.
  • ADAPTABLE web-server development (http://gec.u-picardie.fr/adaptable/index.html). Anticancer and Antimicrobial peptide alignment and clustering.

 

Skills

Multidimensional NMR, biomolecular interactions, protein structure and dynamics,  paramagnetic probes, quadrupolar nuclei, relaxation dispersion, bioinformatics.

 

Roles and responsabilities

Supervisor of two PhD students.

Teaching in Structural Biology, NMR, Biochemistry, Enzymology and Food science at bachelor and master levels.

 

General information

Education and work experience:

  • 2016: Professorship in Biochemistry at GEC, UPJV (Université de Picardie Jules Verne), Amiens, France
  • 2014-2016: Senior research associate, IMDEA (Institutos Madrileños of Estudios Avanzados)/ CNB (National Center Biotechnology), Madrid, Spain
  • 2013-2014: Senior researcher, Computational Biophysics Group, UCL (University College London) 2011-2013 Staff scientist, Computational Biophysics Group, Spanish National Institute for Cancer Research, CNIO (Centro Nacional Investigaciones Oncológicas), Madrid, Spain
  • 2007-2011: Research scientist, Bracco Imaging pharma, Research Center Trieste, Area Science Park, Italy
  • 2004-2007: Postdoctoral Research fellow, CERM (Centro Risonanze Magnetiche), Florence Italy
  • 2000-2004: Postdoctoral Research fellow, Chemistry Department, University of Siena, Siena, Italy
  • 1999: Chemical Sciences. Ph.D.: “The folding pattern of cytochrome b562: the relevance of NMR and dynamic studies on the apo-protein”, (work developed in Robert Kaptein’s lab at Utrecht University, The Netherlands), University of Perugia, Italy

Personal interests

Painting (www.nicoladamelio.com)

 

 

 

Articles

Ramos-martin F., Annaval T., Buchoux S., Sarazin C., D'amelio N. ADAPTABLE: a comprehensive web platform of antimicrobial peptides tailored to the user’s research . Life-science-alliance.org , 2020, 2 ,e201900512 .
 

 

Catherine SARAZIN

PR UPJV - Deputy director

            Contact Catherine Sarazin

 

 

 

Articles

Huet G.,  araya Farias M.,  alayaoubi R., Laclef S., Bouvier, B.  gosselin I.,  cézard C., Roulard R., Courty M., Hadad C.,  husson E.,  sarazin C., Nguyen Van Nhien a. New Biobased-Zwitterionic Ionic Liquids: Efficiency and Biocompatibility for the Development of Sustainable Biorefinery Processes. . Green Chem. , 2020, 22 ,2935 .
 

Ramos-martin F., Annaval T., Buchoux S., Sarazin C., D'amelio N. ADAPTABLE: a comprehensive web platform of antimicrobial peptides tailored to the user’s research . Life-science-alliance.org , 2020, 2 ,e201900512 .
 

Huet G., Hadad C., Husson E., Laclef S., Lambertyn V., Araya Farias M., Jamali A., Courty M., Alayoubi R., Gosselin I., Sarazin C. And Van Nhien A. Straightforward extraction and selective bioconversion of high purity chitin from Bombyx eri larva: Toward an integrated insect biorefinery . Carbohydr. Polym. , 2020, 228,115382 .
 

Araya-farias M., Husson E., Saavedra-torrico J., Gérard D., Roulard R., Gosselin I., Rakotoarivonina H., Lambertyn V. Rémond C. Sarazin C. Wheat Bran Pretreatment by Room Temperature Ionic Liquid-Water Mixture: Optimization of Process Conditions by PLS-Surface Response Design . Fontiers in Chemistry , 2019, 7 ,585.
 

Furlan A., Laurin Y., Botcazon C., Rodríguez-moraga N., Rippa S., Deleu M., Lins L., Sarazin C., Buchoux s. Semi-purified rhamnolipid mixes protect Brassica napus against Leptosphaeria maculans early infections . Phytopathology , 2019, 110(4) ,834-842 .
 

Monnier N., Furlan A-l., Buchoux S., Deleu M., Dauchez M., Rippa S., Sarazin C. Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies" has been published in IJMS as part of the Special Issue SAR "Out of the Box. Int. J. Mol. Sci., 2019, 20,1009.
 

Rondeau M., Esmaeel Q., Crouzet J., Blin Pauline., Gosselin I., Sarazin C., Pernes M., Beugrand J., Wisniewski-dyé F., Vial L., Faure D. Clément C., Ait Barka E., Jacquard C. Sanchez L. Biofilm constructing variants of Paraburkholderia phytofirmans PsJN outcompete the wild-type form in free-living and static conditions but not in planta . Applied and Environmental Microbilogu , 2019, 85 ,e02670-18 .
 

Husson E., Hulin L., Hadad C., Boughanmi C., Stevanovic T., Sarazin C. Acidic Ionic Liquid as Both Solvent and Catalyst for Fast Chemical Esterification of Industrial Lignins: Performances and Regioselectivity . Fontiers in Chemistry , 2019, 7 ,578.
     ABSTRACT: 

Alayoubi R., Mehmood N., Husson E., Kouzayha A., Tabcheh M., Chaveriat L, Sarazin C., Gosselin I. Low temperature ionic liquid pretreatment of lignocellulosic biomass to enhance bioethanol yield . Renewable Energy , 2019, 145 ,1808-1816 .
 

Furlan A., Buchoux S., Miao Y., Banchet V., Leteve M., Lambertyn V. Michel J., Sarazin C., Bonnet V Nanoparticles based on lipidyl-?-cyclodextrins: synthesis, characterization, and experimental and computational biophysical studies for encapsulation of atazanavir. New journal of Chemistry, 2018, 14,112.
     ABSTRACT: Amphiphilic cyclodextrins were synthesized from permethylated ?CD with the aim of forming nanoparticles (NPs) that would encapsulate specific molecules (e.g. drugs) which could enhance their otherwise poor bioavailability. By grafting different fatty acids, four amphiphilic CDs were obtained. The self-assembling properties of three of these compounds were evaluated demonstrating micromolar critical aggregation concentration (CAC). Additionally, the stability of these nanoparticles was studied revealing that the compounds with C18 chains could be stored at 4

Monnier M., Furlan A., Botcazon C., Dahi A., Mongelard G., Cordelier S., Clement C., Dorey S., Sarazin C., Rippa S. Rhamnolipids from Pseudomonas aeruginosa are elicitors triggering Brassica napus protection against Botrytis cinerea without physiological disorders. Frontiers in Plant Science, 2018, 1778,101-124.
     ABSTRACT: Rhamnolipids (RLs) are amphiphilic molecules naturally produced by some bacteria with a large range of biological activities. Although some studies report their potential interest in plant protection, evaluation of their effects and efficiency on annual crops of worldwide agronomic interest is lacking. The main objective of this work was to investigate their elicitor and protective activities on rapeseed crop species while evaluating their physiological effects. Here we report that RLs from Pseudomonas aeruginosa secretome trigger an effective protection of Brassica napus foliar tissues toward the fungus Botrytis cinerea involving the combination of plant defense activation and direct antimicrobial properties. We demonstrated their ability to activate canonical B. napus defense responses including reactive oxygen species production, expression of defense genes, along with callose deposits and stomatal closure as efficient physical protections. In addition, microscopic cell death observations and electrolyte leakage measurements indicated that RLs trigger a hypersensitive response-like defense in this plant. We also showed that foliar spray applications of RLs do not induce deleterious physiological consequences on plant growth or chlorophyll content and that RL protective properties are efficient on several grown cultivars of rapeseed. To our knowledge, this is the first report of RLs as an elicitor that suppresses fungal disease on tissues of an annual crop species under greenhouse conditions. Our results highlight the dual mode of action of these molecules exhibiting plant protection activation and antifungal activities and demonstrate their potential for crop cultures as environmental-friendly biocontrol solution.

Husson E., Auxenfans T., Herbaut M., Baralle M., Lambertyn V., Rakotoarivonina H., Remond C. & Sarazin C. Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases.. Bioresource Technol., 2018, 251,280-287.
     ABSTRACT: Sequential and simultaneous strategies for fractioning wheat straw were developed in combining 1-ethyl-3-methyl imidazolium acetate [C2mim][OAc], endo-xylanases from Thermobacillus xylanilyticus and commercial cellulases. After [C2mim][OAc]-pretreatment, hydrolysis catalyzed by endo-xylanases of wheat straw led to efficient xylose production with very competitive yield (97.6?

Mehmood N., Alayoubi R., Husson E., Jacquard C., Buchs J., Sarazin C. & Gosselin I. Kluyveromyces marxianus, an attractive yeast for ethanolic fermentation in the presence of imidazolium ionic liquids.. Int. J. Molec. Sci., 2018, 19,887.
     ABSTRACT: Imidazolium ionic liquids (ILs) are promising solvents for lignocellulosic biomass (LCB) pretreatment and allow the achievement of higher ethanolic yields after enzymatic hydrolysis and ethanolic fermentation. However, residual ILs entrapped in pretreated biomass are often toxic for fermentative microorganisms, but interaction mechanisms between ILs and cells are still unknown. Here we studied the effects of 1-ethyl-3-methylimidazolium acetate [Emim][OAc] and 1-ethyl-3-methylimidazolium methylphosphonate [Emim][MeO(H)PO2] on Kluyveromyces marxianus, a thermotolerant ethanologenic yeast. Morphological impacts induced by ILs on K. marxianus were characterized by Scanning Electron Microscopy analysis and showed wrinkled, softened, and holed shapes. In Yeast-Malt-Dextrose (YMD) medium, K. marxianus tolerated IL additions up to 2% for [Emim][OAc] and 6% for [Emim][MeO(H)PO2]. Below these thresholds, some IL concentrations enhanced ethanolic yields up to +34% by switching the metabolic status from respiratory to fermentative. Finally, K. marxianus fermentation was applied on several substrates pretreated with [Emim][OAc] or [Emim][MeO(H)PO2] and enzymatically hydrolyzed: a model long fiber cellulose and two industrial LCBs, softwood (spruce) and hardwood (oak) sawdusts. The maximum ethanolic yields obtained were 1.8 to 3.9 times higher when substrates were pretreated with imidazolium ILs. Therefore K. marxianus is an interesting fermentative yeast in a second-generation bioethanol process implying IL pretreatment

Husson E., Hadad C., Huet G., Laclef S., Lesur D., Lambertyn V., Jamali A., Gottis S., Sarazin C., Nguyen Van Nhien A. The effect of room temperature ionic liquids on the selective biocatalytic hydrolysis of chitin via sequential or simultaneous strategies. Greeen Chemistry, 2017, 19,4122-4131.
     ABSTRACT: An efficient conversion of chitin, the second most abundant renewable polymer on the Earth, into N-acetylglucosamine and N,N?-diacetylchitobiose, using room temperature ionic liquids (RTILs) and commercially available chitinases is described for the first time. The sequential strategy consists of the use of RTILs to pretreat chitin under mild conditions as a first step before enzymatic hydrolysis. [C2mim][OAc] (1-ethyl-3-methyl imidazolium) pretreatment provides an efficient production of N-acetylglucosamine (185.0

Auxenfans T., Husson E. & Sarazin C. Simultaneous pretreatment and enzymatic saccharification of (ligno)celluloses in aqueous-ionic liquid media: a compromise.. Biochem. Eng. J., 2017, 117,77-86.
     ABSTRACT: In view of decreasing the amount of IL to achieve efficient simultaneous pretreatment and saccharification, a comprehensive study was undertaken. Different types of lignocellulosic biomasses were investigated in various enzymatic aqueous-IL systems including 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) or 1-ethyl-3-methylimidazolium methylphosphonate ([C2mim][MeO(H)PO2]). To better understand how 10% (v/v) of IL in the reaction medium led to the highest yields of glucose without fractionation from sawdust, distinct cellulosic models were then used as substrates. Kinetic studies demonstrated that both ILs affect synergistic action of cellulolytic enzymes depending on both constitutive anion and cellulosic substrate. Concentrations above 10% v/v of ILs deactivated cellulase even on highly digestible model substrates. ? and ? Kamlet-Taft parameters constituted more physicochemical pertinent indicators than apparent pH value to investigate effects of IL on cellulase performances. Fine description of these effects was proposed onto individual EG, CBH and BG. [C2mim][MeO(H)PO2] was demonstrated a better compatible IL for enzymes up to a concentration of 30% (v/v). The efficiency of simultaneous pretreatment and saccharification was governed by a compromise between better substrate accessibility and enzyme deactivation.

Brahim M., Checa Fernandez B. L., Regnier O., Bousseta N., Grimi N., Sarazin C., Husson E., Vorobiev E., Brosse N. Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straw’s lignin and pulps. Bioresource Technology, 2017, 237,42308
  

Nail Nasir M., Lins L., Crowet J. M., Ongena M., Dorey S., Dhondt-cordelier S., Clement C., Bouquillon S., Haudrechy A., Sarazin C., Fauconnier M. L., Nottk., Deleu M. Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straw’s lignin and pulps. Langmuir, 2017, 33,9979-9987.
     ABSTRACT: Natural and synthetic amphiphilic molecules including lipopeptides, lipopolysaccharides, and glycolipids are able to induce defense mechanisms in plants. In the present work, the perception of two synthetic C14 rhamnolipids, namely, Alk-RL and Ac-RL, differing only at the level of the lipid tail terminal group have been investigated using biological and biophysical approaches. We showed that Alk-RL induces a stronger early signaling response in tobacco cell suspensions than does Ac-RL. The interactions of both synthetic RLs with simplified biomimetic membranes were further analyzed using experimental and in silico approaches. Our results indicate that the interactions of Alk-RL and Ac-RL with lipids were different in terms of insertion and molecular responses and were dependent on the lipid composition of model membranes. A more favorable insertion of Alk-RL than Ac-RL into lipid membranes is observed. Alk-RL forms more stable molecular assemblies than Ac-RL with phospholipids and sterols. At the molecular level, the presence of sterols tends to increase the RLs

Brahim M., Ferandez B. L. C., Regnier O., Boussetta N., Grimi N., Sarazin C., Husson E., Vorobiev E., Brosse N Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straw's lignin and pulps. Bioressource Technology , 2017, 237,11-19.
     ABSTRACT: In this study, ultrasound (US) and high voltage electrical discharges (HVED) were combined with chemical treatments (soda or organosolv) for rapeseed straw delignification. Delignification was improved by both physical pretreatments. US increased the extractability of hemicelluloses and HVED induced a partial degradation of cellulose. Best synergies were observed for HVED-soda and US-organosolv treatments. The obtained lignin fractions were characterized with 13C NMR and 2D 1H

Mehmood N., Husson E., Jacquard C., Wewetzer S., Buchs J., Sarazin C. & Gosselin I. Impact of two ionic liquids, 1-ethyl-3-methylimidazolium acetate and 1-ethyl-3-methylimidazolium methylphosphonate, on Saccharomyces cerevisiae: metabolic, physiologic, and morphological investigations.. Biotechnol. Biofuels , 2015, 8,n.17.
 

Bonnet V., Gervaise C., Djedaini-pilard F., Furlan A. & Sarazin C. Cyclodextrin nanoassemblies: a promising tool for drug delivery.. Drug. Discov. Today, 2015, 20,1120-1126.
 

Hulin L., Husson E., Bonnet J. P., Stevanovic T. & Sarazin C. Enzymatic transesterification of kraft lignin with long acyl chains in ionic liquids.. Molecules, 2015, 20,16334-16353.
 

Bompart M., Goto A., Wattraint O., Sarazin C., Tsujii Y., Gonzato C. & Haupt K. Molecularly imprinted polymers by reversible chain transfer catalyzed polymerization.. Polymer, 2015, 78,31-36.
 

Auxenfans T., Buchoux S., Larcher T., Husson G., Husson E. & Sarazin C. Enzymatic saccharification and structural properties of industrial wood sawdust: recycled ionic liquids pretreatments.. Energ. Convers. Manage., 2014, 88,1094-1103.
 

Gervaise C., Bonnet V., Nolay F., Cezard C., Stasik I., Sarazin C. & Djedaini-pilard F. Diesterification of 3-[(ß-cyclodextrinyl)succinamido]propane-1,2-diol catalyzed by lipase: Diastereoselectivity or tridimensional substrate specificity?. Eur. J. Org. Chem., 2014, 2014,6200-6209.
 

Auxenfans T., Buchoux S., Husson E. & Sarazin C. Efficient enzymatic saccharification of Miscanthus: energy-saving by combinig dilute acid and ionic liquid pretreatments.. Biomass Bioenerg., 2014, 62,82-92.
 

Bonnet V., Favrelle A., Aubry F., Sarazin C. & Djedaini-pilard F. Solvent-free chemo-enzymatic synthesis of fatty acyl-bcyclodextrin.. J. Incl. Phenom. Macrocycl. Chem., 2013, 77,155-161.
 

Wattraint O., Saadallah I., Silva-peres V., Sonnet P. & Sarazin C. Influence of the insertion of a cationic peptide on the size and shape of nanoliposomes: a light scattering investigation.. Int. J. Pharm., 2013, 454,621-624.
 

Gervaise C., Bonnet V., Wattraint O., Aubry F., Sarazin C., Jaffres P. A. & Djedaini-pilard F. Synthesis of lipophosphoramidyl-cyclodextrins and their supramolecular properties.. Biochimie, 2012, 94,66-74.
 

Auxenfans T., Buchoux S., Djellab K., Avondo C., Husson E. & Sarazin C. Mild pretreatment and enzymatic saccharification of cellulose with recycled ionic liquid towards one-batch process.. Carbohydr. Polym., 2012, 90,805-813.
 

Gervaise C., Bonnet V., Sarazin C. & Djedaini-pilard F. Synthesis of glycerolipidyl derivatives of permethylated b-cyclodextrin as potential nanovectors.. New J. Chem., 2012, 36,2417-2424.
 

Husson E., Buchoux S., Avondo C., Cailleu D., Djellab K., Gosselin I., Wattraint O. & Sarazin C. Enzymatic hydrolysis of ionic liquid-pretreated celluloses: contribution of CP-MAS, 13C NMR and SEM.. Bioresource Technol., 2011, 102,7335-7342.
 

Bonnet V., Gervaise C., Favrelle A., Sarazin C. & Djedaini-pilard F. Enzymatic catalysis in presence of cyclodextrins.. Curr. Org. Chem., 2010, 14,1323-1336.
 

Favrelle A., Bonnet V., Avondo C., Aubry F., Djedaini-pilard F. & Sarazin C. Lipase-catalyzed synthesis and characterization of novel lipidyl-cyclodextrins in solvent free medium.. J. Mol. Catal. B., 2010, 66,224-227.
 

Kouzayha A., Wattraint O. & Sarazin C. Interactions of two transmembrane peptides in supported lipid bilayers studied by a 31P and 15N MAOSS NMR strategy.. Biochimie, 2009, 91,774-778.
 

Kouzayha A., Nasir M. N., Buchet R., Wattraint O., Sarazin C. & Besson F. Conformational and interfacial analyses of K3A18K3 and alamethicin in model membranes.. J. Phys. Chem. B, 2009, 113,7012-7019.
 

Favrelle A., Bonnet V., Sarazin C. & Djedaini-pilard F. Synthesis of polyenyl derivatives of permethylated beta-cyclodextrin.. Tetrahedron Asym., 2008, 19,2240-2245.
 

Favrelle A., Bonnet V., Sarazin C. & Djedaini-pilard F. Novel chemo-enzymatic access to amphiphilic cyclodextrins. . J. Incl. Phenom. Macrocycl. Chem., 2007, 57,15-20.
 

Wattraint O. & Sarazin C. Static and MAS solid-state study of supported phospholipid bilayer cylindrically oriented. . C.R. Chimie, 2006, 9,408-412.
 

Patent

E. Husson, C. Sarazin, H. Ducatel, I. Murrieta-pazos, G. Ravot Procédé de traitement de la biomasse. , 2017, ,.
 

Claire ROSSI

PR UTC - Manager Food Science Platform

          Contact Claire Rossi

 

Research

I’m working at the interface between the theme “Plant Metabolism and Bioresources” and “Biomimicry and Biomolecular Recognition”. My activities cover both fundamental and applied research.

Concerning fundamental research activities, I am particularly interested in studying the biological mode of action and health potential of phytochemicals found in food. My research deals with the development of sensors and the study of molecular interactions. It lies more particularly in the interactions occurring at the cell membrane interface. I developed the first in vitro systems ever reported for studying the protein translocation across biomimetic cell membranes as well as for the transmembrane protein dimerization and their consecutive activation.

Concerning applied research activities, I developed and lead a technology development and transfer platform in food science. The knowledge gained in nutritional biochemistry is applied for developing prototypes of innovative food products for companies. I built a strong experience in the nutritional optimisation of food products and in providing a healthy and bioactive added-value to food products. In 2018, a team of students that I led won the gold trophy of the competition Ecotrophelia France and the special price for the best innovation at Ecotrophelia Europe.
Skills

Characterization of molecular interactions; Study of the drug or phytochemical interactions with cells; Study of the bioactive properties of phytochemicals; Development and application of biosensors; Development of cell membrane models.

Food science; Food formulation; Food biochemistry; Nutritional optimization; Development of innovative food prototypes.
Roles and responsabilities

  • Since 2017: Elected member of the board of directors of the University of Technology de Compiègne. Alliance Sorbonne University.
  • Since 2015: Head of the Food Science platform. Innovation Center. UTC.
  • Since 2012: Head of the Major Innovation in Agrifood. Specialty of engineering diploma. Department of biological engineering, UTC.
  • Since 2013: Coordinator of the organic chemistry teaching module. UTC.
  • 2009-2017: Elected member of the Scientific Board of the Department of Biological Engineering CNRS, UTC.
  • Since 2007: Coordinator of the food formulation teaching module. UTC.

 

General information

  • Since Sept. 2016: Professor. Laboratory of cell and enzyme engineering CNRS UMR 7025. Department of biological engineering, University of Technology of Compiegne (UTC).
  • 2014-2016: Associate professor. CNRS UMR 7025, UTC.
  • April 2014: ‘Habilitation à diriger des recherches’ (Habilitation to conduct researches). UTC.
  • 2007-2014: Assistant professor. CNRS UMR 7025, UTC.
  • 2006-2007: Postdoctoral Research Associate. Max Planck Institute for Polymer Research, Mainz, Germany.
  • 2002-2006: PhD in biochemistry and biophysics. CNRS UMR 7025, UTC.
  • September 2002: M.Sc. in biochemistry. National High School of Chemistry of Toulouse ENSIACET, INP.
  • August 2002:Engineer diploma in organic chemistry. National High School of Chemistry of Toulouse, ENSIACET, INP.

 

Personal interests

  • Consulting in food formulation and in experimental design
  • Scientific advising for spin-off companies
  • Competitions in food innovation
  • Conduction of  public workshops devoted to the application of nutrition and food science knowledge
  • Writer for the website www.lanutrition.fr

 

 

Articles

Horstmann J. A., Lunelli M., Cazzola H., Heidemann J., Kühne C., Steffen P., Szefs S.,  rossi C., Lokareddy Rk., Wang C., Lemaire L., Hughes Kt., Uetrecht C., Schlüter H., Grassl Ga., Stradal Teb., Rossez ., Kolbe M., Erhardt M. Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion . Nat Commun , 2020, 11 ,2013 .
 

Imatoukene N., Back A., Nonus M.,  thomasset B., Rossignol T. Et Nicaud J. M. Fermentation process for producing CFAs using Yarrowia lipolytica . Journal of Industrial Microbiology and Biotechnology , 2020, 47 ,403-412 .
 

Czerwic Q., Idrissitaghki A., Imatoukene N., Nonus M., Thomasset B., Nicaud J-m., Rossignol T. Optimization of cyclopropane fatty acids production in Yarrowia lipolytica. Yeast, 2019, 36,143-151.
     ABSTRACT:  Cyclopropane fatty acids, which can be simply converted to methylated fatty acids, are good unusual fatty acid candidates for long‐term resistance to oxidization and low‐temperature fluidity useful for oleochemistry and biofuels. Cyclopropane fatty acids are present in low amounts in plants or bacteria. In order to develop a process for large‐scale biolipid production, we expressed 10 cyclopropane fatty acid synthases from various organisms in the oleaginous yeast Yarrowia lipolytica, a model yeast for lipid metabolism and naturally capable of producing large amounts of lipids. The Escherichia coli cyclopropane fatty acid synthase expression in Y. lipolytica allows the production of two classes of cyclopropane fatty acids, a C17:0 cyclopropanated form and a C19:0 cyclopropanated form, whereas others produce only the C17:0 form. Expression optimization and fed‐batch fermentation set‐up enable us to reach a specific productivity of 0.032 g·L−1·hr−1 with a genetically modified strain containing cyclopropane fatty acid up to 45% of the total lipid content corresponding to a titre of 2.3 ± 0.2 g/L and a yield of 56.2 ± 4.4 mg/g.

Horstmann J. A., Lunelli M., Cazzola H., Heidmann J., Kühne C., Steffen P., Szefs S., Rossi C., Lokareddy R. K., Wang C., Hughes T., Uetrecht C., Schlüter H., Grassi G. A., Stradal T. E. B., Rossez., Kolbe M., Erhardt M. Flagella methylation promotes bacterial adhesion and host cell invasion . Biorxiv , 2019, ,.
 

Rossi C., Cazzola H., Holden N. J, Rossez Y. Bacterial Adherence to Plant and Animal Surfaces Via Adhesin-Lipid Interactions . Springer, Cham , 2019, ,1-21.
 

Veneziano R., Rossi C., Chenal A., Brenner C., Ladant D., Chopineau J. Synthesis and characterization of tethered lipid assemblies for membrane protein reconstitution. Biointerphases, 2017, 12,04E301.
     ABSTRACT: Biological membranes and their related molecular mechanisms are essential for all living organisms. Membranes host numerous proteins and are responsible for the exchange of molecules and ions, cell signaling, and cell compartmentation. Indeed, the plasma membrane delimits the intracellular compartment from the extracellular environment and intracellular membranes. Biological membranes also play a major role in metabolism regulation and cellular physiology (e.g., mitochondrial membranes). The elaboration of membrane based biomimetic systems allows us to reconstitute and investigate, in controlled conditions, biological events occurring at the membrane interface. A whole variety of model membrane systems have been developed in the last few decades. Among these models, supported membranes were developed on various hydrophilic supports. The use of solid supports enables the direct use of surface sensitive techniques (e.g., surface plasmon resonance, quartz crystal microbalance, and atomic force microscopy) to monitor and quantify events occurring at the membrane surface. Tethered bilayer membranes (tBLMs) could be considered as an achievement of the first solid supported membranes described by the McConnell group. Tethered bilayers on solid supports were designed to delimit an inside compartment from an outside one. They were used for measuring interactions with ligands or incorporating large membrane proteins or complexes without interference with the support. In this context, the authors developed an easy concept of versatile tBLMs assembled on amino coated substrates that are formed upon the vesicle fusion rupture process applicable to protein-free vesicles as well as proteoliposomes. The phospholipid bilayer (natural or synthetic lipids) incorporated 5% of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-poly ethylene glycol-N-hydroxy succinimide to ensure the anchorage of the bilayer to the amino coated surface. The conditions for the formation of tBLMs on amino-coated gold and glass were optimized for protein-free vesicles. This biomimetic membrane delimits an inside

Hodrogue A., Trecherel E., Cornu M., Darwiche W., Mansour A., Ait-mohand K., Verissimo T., Gomila C., Schembri C., Da Nascimento S., Elboutachfait R., Boulier A., Lorne E., Courtois J., Petit E., Toumieux S., Kovensky J., Sonnet P., Massy Z. A., Kamel S. Rossi C., Ausseil J. Oligogalacturonic acid inhibits vascular calcification by two mechanisms: inhibition of VSMC osteogenic conversion and interaction with collagen. Arteriosclerosis, Thrombosis, and Vascular Biology, 2017, 37,1391-1401.
 

Xu J. J., Ambrosini S., Tamahkar E., Rossi C., Haupt K. & Tse Sum Bui B. Toward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins.. Biomacromolecules, 2016, 17,345-353.
 

Nestora S., Merlier F., Prost E., Haupt K., Rossi C. & Tse Sum Bui B. Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer.. J. Chromatogr. A, 2016, 1465,47-54.
 

Starok M., Preira P., Vayssade M., Haupt K., Salome L. & Rossi C. EGFR inhibition by curcumin in cancer cells: a dual mode of action.. Biomacromolecules, 2015, 16,1634-1642.
 

Nowacki L., Follet J., Vayssade M., Vigneron P., Cambay F., Egles C. & Rossi C. Real-time QCM-D monitoring of cancer cell death events in a dynamic context.. Biosens. Bioelectron., 2015, 64,469-476.
 

Fabre G., Bayach I., Berka K., Paloncyova M., Starok M., Rossi C., Duroux J. L., Otyepka M. & Trouillas P. Synergism of antioxidant action of vitamins E and Quercetin is related to formation of molecular associations in biomembranes.. Chem. Commun., 2015, 51,7713-7716.
 

Nowacki L., Vigneron P., Rotellini L., Cazzola H., Merlier F., Prost E., Ralanairina R., Gadonna J. P., Rossi C. & Vayssade M. Betanin-enriched red beetroot (Beta vulgaris L.) extract induces apoptosis and autophagic cell death in MCF-7 cells.. Phytother. Res., 2015, 29,1964-1973.
 

Vidal G., Blanchi T., Mieszawska A. J., Calabrese R., Rossi C., Vigneron P., Duval J. L., Kaplan D. L. & Egles C. Enhanced cellular adhesion on titanium by silk functionalized with titanium binding and RGD peptides.. Acta Biomater., 2013, 9,4935-4943.
 

Crosson C. & Rossi C. Quartz crystal microbalance immunosensor for the quantification of Immunoglobulin G in bovine milk.. Biosens. Bioelectron., 2013, 42,453-459.
 

Veneziano R., Rossi C., Chenal A., Devoiselle J. M., Ladant D. & Chopineau J. Bordetella pertussis Adenylate Cyclase Toxin Translocation across a Tethered Lipid Bilayer.. Proc. Natl. Acad. Sci. USA, 2013, 110,20473-20478.
 

Doumiati S., Haupt K. & Rossi C. Autophosphorylation activation and inhibition by curcumin of the epidermal growth factor receptor reconstituted in liposomes.. J. Mol. Recognit., 2012, 25,623-629.
 

Rossi C., Doumiati S., Lazzarelli C., Davi M., Meddar F., Ladant D. & Chopineau J. A tethered bilayer assembled on top of immobilized calmodulin to mimic cellular compartmentalization.. PloS One, 2011, 6,e19101.
 

Crosson C., Thomas D. & Rossi C. Quantification of immunoglobulin G in bovine and caprine milk using a surface plasmon resonance–based immunosensor.. J. Agr. Food Chem., 2010, 58,3259-3264.
 

Eid M., Rippa S., Castano S., Desbat B., Chopineau J. Rossi C. & Beven L. Exploring the membrane mechanism of the bioactive peptaibol ampullosporin A using lipid monolayers and supported biomimetic membranes. J. Biophys., 2010, 2010,Art.ID 179641.
 

Vockenroth I. K., Rossi C., Raza Sha M. & Koper I. Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques.. Biointerphases, 2009, 4,19-26.
 

Rossi C. & Chopineau J. Biomimetic tethered lipid membranes designed for membrane-protein interaction studies.. Eur. Biophys. J., 2007, 36,955-965.
 

Rossi C., Briand E., Parot P., Odorico M. & Chopineau J. Surface response methodology for the study of supported membrane formation.. J. Phys. Chem. B, 2007, 111,7567-7576.
 

Deniaud A., Rossi C., Berquand A., Homand J., Campagna S., Knoll W., Brenner C. & Chopineau J. Voltage-dependent anion channel transports calcium ions through biomimetic membranes.. Langmuir, 2007, 23,3898-3905.
 

Rossi C. & Haupt K Application of the Doehlert experimental design to molecularly imprinted polymers: surface response optimization of specific template recognition as a function of the type and degree of cross-linking.. Anal. Bioanal. Chem., 2007, 389,455-460.
 

BOOK

Maximilien J., Beyazit S., Rossi C., Haupt K. & Tse Sum Bui B Nanoparticles in biomedical applications. Measuring Biological impacts of nanomaterials, 2016, Springer International Publishing, Switzerland,pp. 177-210
  

Yolande PERRIN

PR UTC

         Contact Yolande Perrin

 

Research

I am studying the enzymatic pathways of intracellular fatty acid trafficking in the context of lipid metabolism of oilseed plants (rape, flax, castor oil, Arabidopsis). On the one hand, my interests go on the implication of carnitine in the plant physiology (Charrier et al., 2012, Jacques et al., 2018), in particular on the intracellular transport of fatty acids during lipids syntheses (Bourdin et al., 2007, Nguyen et al., 2016). On the other hand, I study the enzymatic processes of fatty acid transfer during the synthesis of glycerolipids membrane or reserve, in the context of atypical fatty acid management in particular (Fahs et al., 2019). My research contributes to the knowledge of lipid synthesis modalities in oleaginous plants, and to the identification of transferable functions to biotechnological systems for the production of high added value lipids.

 

Skills
Plant biotechnology, Plant physiology, Lipid metabolism, Molecular biology, Biochemistry

 

Roles and responsabilities
In charge of Engineering Training in Biological Engineering at UTC

  • General information
  • 1986: DEUG, Science of nature and life
  • 1987: Bachelor’s degree in Biology of organisms, biology option
  • 1988: Master’s degree in Biology of organisms and populations, plant physiology option
  • 1989: DEA in Agricultural sciences, Physiology applied to plant productions
  • 1990-1994: PhD in Plant physiology, University of Montpellier 2
  • 1994: Postdoctoral researcher at the University of Picardie Jules Vernes
  • 1995-2001: Postdoctoral researcher at John Innes, Virology department, Molecular Biotechnology, Biology Chemistry department
  • 2001: Assistant Professor at UTC (section CNU 64), UMR CNRS 7025, GEC
  • 2012: HDR, UTC
  • 2016: Professor at UTC, UMR CNRS 7025, GEC

Articles

Fahs Z., Rossez Y., Guénin S., Gutierrez L., Thomasset B., & Perrin, Y. Cloning and molecular characterization of three lysophosphatidic acid acyltransferases expressed in flax seeds. Plant Science, 2019, 280,41-50.
 

Jacques F., Rippa S., Perrin Y. Physiology of L-carnitine in plants in light of the knowledge in animals and microorganisms. Plant Science, 2018, 9,1170.
     ABSTRACT: L-carnitine is present in all living kingdoms where it acts in diverse physiological processes. It is involved in lipid metabolism in animals and yeasts, notably as an essential cofactor of fatty acid intracellular trafficking. Its physiological significance is poorly understood in plants, but L-carnitine may be linked to fatty acid metabolism among other roles. Indeed, carnitine transferases activities and acylcarnitines are measured in plant tissues. Current knowledge of fatty acid trafficking in plants rules out acylcarnitines as intermediates of the peroxisomal and mitochondrial fatty acid metabolism, unlike in animals and yeasts. Instead, acylcarnitines could be involved in plastidial exportation of de novo fatty acid, or importation of fatty acids into the ER, for synthesis of specific glycerolipids. L-carnitine also contributes to cellular maintenance though antioxidant and osmolyte properties in animals and microbes. Recent data indicate similar features in plants, together with modulation of signaling pathways. The biosynthesis of L-carnitine in the plant cell shares similar precursors as in the animal and yeast cells. The elucidation of the biosynthesis pathway of L-carnitine, and the identification of the enzymes involved, is today essential to progress further in the comprehension of its biological significance in plants.

Nguyen P. J., Rippa S., Rossez Y. & Perriny. Acylcarnitines participate in developmental processes associated to lipid metabolism in plants.. Planta, 2016, 243,1011-1022.
 

Rippa S., Zhao Y., Merlier F., Charrier A. & Perrin Y. The carnitine biosynthetic pathway in Arabidopsis thaliana shares similar features with the pathway of mamals and fungi.. Plant Physiol. Biochem., 2012, 60,109-114.
 

Charrier A., Rippa S., Yu A., Nguyen P. J., Renou J. P. & Perrin Y. The effect of carnitine on Arabidopsis development and recovery in salt stress conditions.. Planta, 2012, 235,123-135.
 

Picard N., Guenin S., Perrin Y., Hilaire G. & Larnicol N. Consequences of prenatal exposure to diazepam on the respiratory parameters, respiratory network activity and gene expression of a1 and alpha-2 subunits of GABA(A) receptor in newborn rat.. Adv. Exp. Biol. Med., 2008, 605,144-148.
 

Picard N., Guenin S., Perrin Y. & Larnicol N. Maternal caffeine ingestion during gestation and lactation influences respiratory adaptation to acute alveolar hypoxia in newborn rats and adenosine A2A and GABA A receptor mRNA transcription.. Neuroscience, 2008, 156,630-639.
 

Picard N., Guenin S., Perrin Y., Hilaire G. & Larnicol N. Prenatal diazepam exposure alters respiratory control system and Gabaa and adenosine receptor gene expression in newborn rats.. Pediatr. Res., 2008, 64,44-49.
 

Lelandais-briere C., Jovanovic M., Torres G. A., Perrin Y., Lemoine R., Corre-menguy F. & Hartmann C. Disruption of AtOCT1, an organic cation transporter gene, affects root development and carnitine-related responses in Arabidopsis.. Plant J., 2007, 51,154-164.
 

Bourdin B., Adenier H. & Perrin Y. Carnitine is associated with fatty acid metabolism in plants.. Plant Physiol. Biochem., 2007, 45,926-931.
 

Alamillo J. M., Monger W., Sola I., Garcia B., Perrin Y., Bestagno M., Burrone O. R., Plana-duran J., Enjuanes L., Lomonossoff G. P. & Garcia J. A. Use of virus vectors for the expression in plants of active full-length and single-chain anti-coronavirus antibodies. . Biotechnol. J., 2006, 1,1103-1111.
 

Canizares M. C., Liu L., Perrin Y., Tsakiris E. & Lomonossoff G. P. A bipartite system for the constitutive and inducible expression in high levels of foreign proteins in plants. . Plant Biotechnol. J., 2006, 4,183-193.
 

Monger W., Alamillo J. M., Sola I., Perrin Y., Bestagno M., Burrone O. R., Sabella P., Plana-duran J., Enjuanes L., Garcia J. A. & Lomonossoff G. P. An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus infection when supplied orally in crude plant extracts. . Plant Biotechnol. J., 2006, 4,623-631.
 

Séverine PADIOLLEAU

MC UTC

       Contact Séverine Padiolleau

 

Research

Member of the theme “Biomimicry and Biomolecular Diversity”.

Genesis and exploitation of the molecular diversity for fundamental and applied purposes. Research activity focuses on the diversity of the immune repertoire, the place and roles of catalytic antibodies in autoimmune pathologies. Synthesis and exploitation of antibodies libraries displayed on phage surface. Selection of specific ligands against identified target with regulation, detection or quantification applications. Antibodies engineering.

 

Skills

Cloning technics (PCR, RACE-PCR, mutagenesis, transformations…). Biochemical technics (enzymology, ELISA, Western Blots, Competition tests, SPR, recombinant proteins expression, refolding, cell culture…)

 

Roles and responsabilities

Supervision of students (PhD, masters, engineers). Project leader or member in various scientific programs (Sorbonne Université, FEDER, ANR, La Ligue contre le Cancer,…). Teaching in engineers school (UTC) and doctoral school. Courses leader (“Metabolism and cellular physiology”, course for L3 students, and “Life science for the Engineer” for L1 to L2 students).

International relationships Coordinator for the Biological Engineering Department.

 

General information

Since 2006, senior lecturer (section CNU 64) at University of Technology of Compiègne.

HDR (2013) “Understanding catalytic antibodies origin : Genetic analysis and Engineering”. PhD defensed in 2004 “biochemical and molecular characterization of an antiidiotypic antibody displaying a betalactamase like activity”.

 

Articles

Shahsavarian M., Chaaya N., Costa N., Boquet D., Atkinson A., Offmann B., Kaveri Sv., Lacroix-desmazes S., Friboulet A., Avalle B. & Padiolleau-lefevre S. Multi-target selection of catalytic antibodies wih . FEBS J., 2017, 284,634-653.
     ABSTRACT: ??lactamase enzymes responsible for bacterial resistance to antibiotics are among the most important health threats to the human population today. Understanding the increasingly vast structural motifs responsible for the catalytic mechanism of ??lactamases will help improve the future design of new generation antibiotics and mechanism?based inhibitors of these enzymes. Here we report the construction of a large murine single chain fragment variable (scFv) phage display library of size 2.7 ? 109 with extended diversity by combining different mouse models. We have used two molecularly different inhibitors of the R?TEM ??lactamase as targets for selection of catalytic antibodies with ??lactamase activity. This novel methodology has led to the isolation of five antibody fragments, which are all capable of hydrolyzing the ??lactam ring. Structural modeling of the selected scFv has revealed the presence of different motifs in each of the antibody fragments potentially responsible for their catalytic activity. Our results confirm (a) the validity of using our two target inhibitors for the in vitro selection of catalytic antibodies endowed with ??lactamase activity, and (b) the plasticity of the ??lactamase active site responsible for the wide resistance of these enzymes to clinically available inhibitors and antibiotics.

Mahendra A., Peyron I., Thaunat O., Dollinger C., Gilardin L., Sharma M., Wootla B., Rao D. N., Padiolleau-lefevre S., Boquet D., More A., Varadarajan N., Kaveri S. V., Legendre C. & Lacroix-desmazes S. Generation of catalytic antibodies is an intrinsic property of an individual's immune system: a study on a large cohort of renal transplant patients.. J. Immunol., 2016, 196,4075-4081.
 

Padiolleau-lefevre S., Ben Naya R., Shahsavarian M., Friboulet A. & Avalle B. Catalytic antibodies and their applications in biotechnology: State of the art.. Biotechnol. Lett., 2014, 36,1369-1379.
 

Shahsavarian M., Le Minoux D., Matti K. M., Kaveri S., Lacroix-desmazes S., Boquet D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Exploitation of rolling circle amplification for the construction of large phafe display antibody libraries.. J. Immunol. Methods, 2014, 407,26-34.
 

Ben Naya R., Matti K., Guellier A., Matagne A., Boquet D., Thomas D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Efficient refolding of a recombinant abzyme: Structural and catalytic characterizations.. Appl. Microbiol. Biotechnol., 2013, 97,7721-7731.
 

Mahendra A., Peyron I., Dollinger C., Gilardin L., Sharma M., Wootla B., Padiolleau-lefevre S., Friboulet A., Boquet D., Legendre C., Kaveri S., Thaunat O. & Lacroix-desmazes S. IVIg treatment reduces catalytic antibody titers of renal transplanted patients.. PloS One, 2013, 8,e70731.
 

Mahendra A., Padiolleau-lefevre S., Kaveri S. & Lacroix-desmazes S. Do proteolytic antibodies complete the panopply of the autoimmune response in acquired haemophilia A?. Br. J. Haematol., 2012, 156,3-12.
 

Le Minoux D., Mahendra A., Lacroix-desmazes S., Limnios N., Friboulet A., Avalle B., Boquet D., Kaveri S. & Padiolleau-lefevre S. A novel molecular analysis of genes encoding catalytic antibodies.. Mol. Immunol., 2012, 50,160-168.
 

Phichith D., Bun S., Padiolleau-lefevre S., Guellier A., Banh S., Gallieni M., Frere J. M., Thomas D., Friboulet A. & Avalle B. Novel peptide inhibiting both TEM-1 beta-lactamase and penicillin-binding proteins.. FEBS J., 2010, 277,4965-4972.
 

Essono S., Clement G., Padiolleau-lefevre S., Creminon C., Grassi J. & Boquet D. Peptide mass assisted antibody cloning strategy for accurate characterization of potential therapeutic monoclonal antibodies against neurodegenerative diseases.. Prot. Eng. Des. Select., 2010, 23,203-210.
 

Phichith D., Bun S., Padiolleau-lefevre S., Banh S., Thomas D., Friboulet A. & Avalle B. Mutational and inhibitory analysis of a catalytic antibody. Implication for drug discovery.. Mol. Immunol., 2009, 47,348-356.
 

Padiolleau-lefevre S., Debat H., Phichith D., Thomas D., Friboulet A. & Avalle B. Expression of a functional scFv fragment of an anti-idiotypic antibody with a ß-lactam hydrolytic activity. . Immunol. Letters, 2006, 103,39-44.
 

Chaaya N., Shahsavarian M., Maffucci I., Friboulet A., Offmann B., Leger J. B., Rousseau S., Avalle B., Padiolleau-lefevre S. Genetic background and immunological status influence B cell repertoire diversity in mice . Scientific Reports , 0, 9,14261 .
 

Patent

B. Avalle-bihan, A. Friboulet, H. Isber, C. Loussouarn, S. Padiolleau-lefèvre. Inhibiteurs de STAT5 et utilisation de ceux-ci. , 2014, ,.
 

Carlo GONZATO

MC UTC

           Contact Carlo Gonzato

 

 

Research

Member of the theme “Biomimicry and Biomolecular Diversity”.

Synthesis of molecularly imprinted nanomaterials and nanocomposites for biomimetic applications and bio/chemo-sensing. New polymerization techniques for imprinted materials. Synthesis of bio-mimicking functional polymers.

 

Skills

  • Polymer synthesis by radical techniques.
  • Polymer synthesis by bulk, dispersion, precipitation polymerization.
  • Polymer characterization: GPC, NMR, DLS, electrophoresis, rheology.
  • Polymerization-induced self-assembly.
  • Surface functionalization of nanomaterials.
  • Synthesis of composites nanomaterials.

 

General information

  • Current position: Lecturer
  • Research associate (UTC, FR)
  • Research associate (The University of Sheffield, UK)
  • PhD in biotechnology (UTC, FR)
  • R&D scientist (Montefibre SpA, IT)
  • Master degree in Chemistry (University of Padova, IT)

 

Personal interest

Hiking

 

Articles

Leibl N., Duma L., Gonzato C. Et Haupt K. Polydopamine-based molecularly imprinted thin films for electro-chemical sensing of nitro-explosives in aqueous solutions . Bioelectrochemistry , 2020, 135 ,107541 .
 

Garcia-soto M., Haupt K., Gonzato C. Synthesis of molecularly imprinted polymers by photo-iniferter polymerization under visible light.. Polym. Chem., 2017, 8,4830-4834.
     ABSTRACT: The easiest way to make polymers able to specifically recognize a target molecule consists of copolymerizing functional monomers and a cross-linker around a molecular template. This method affords a rigid material bearing high affinity binding sites, namely a synthetic receptor or antibody mimic, known as the

Bonomi P., Attieh M. D., Gonzato C. & Haupt K. A novel versatile water soluble iniferter platform for the preparation of imprinted nanoparticles via photopolymerization in aqueous media.. Chem.-Eur. J., 2016, 22,10150-10154.
 

Beyazit S., Tse Sum Bui B., Haupt K. & Gonzato C. Molecularly imprinted polymer nanomaterials and nanocomposites by controlled/living radical polymerization.. Prog. Polym. Sci., 2016, 62,1-21.
 

Bompart M., Goto A., Wattraint O., Sarazin C., Tsujii Y., Gonzato C. & Haupt K. Molecularly imprinted polymers by reversible chain transfer catalyzed polymerization.. Polymer, 2015, 78,31-36.
 

Gonzato C., Pasetto P., Bedoui F., Mazeran P. E. & Haupt K. On the effect of using RAFT and FRP for the bulk synthesis of acrylic and methacrylic molecularly imprinted polymers.. Polymer Chem., 2014, 5,1313-1322.
 

Gonzato C., Courty M., Pasetto P. & Haupt K. Magnetic molecularly imprinted polymer nanocomposites via surface-initiated RAFT polymerization.. Adv. Funct. Mater., 2011, 21,3947-3953.
 

Aude CORDIN

MC UTC

Contact Aude Cordin

 

Research

Member of the theme “Biomimicry and Biomolecular Diversity”.

Development of molecular imprinted polymers for applications in biosensors and separation sciences.

Nanostructured, nanocomposite, bio-based molecularly imprinted polymers and delivery systems for medical, environmental and food application: degradable and biological-responsive materials, study of polymer biodegradation.

Molecular imprinting polymer synthesis by “green chemistry”.

Transversal research activities with the team “plant metabolism and bioressources” in plant biodiversity exploitation and characterization (polyphenols extraction and antioxidants activities).

 

Skills

Teaching activities in chemistry, biochemistry, enzymology and food formulation.

Scientific skills:

  • Molecular imprinting technology: polymerization methods (FRP, ATRP, precipitation polymerization, enzymatic polymerization…), surface functionalization, nanostructuration (nanomolding, nanocomposites, core-shell particles…).
  • Biological-responsive polymers synthesis, degradation and release properties studies.
  • Biocatalyse, biopolymer and natural products modification.
  • Encapsulation methods: emulsion, ionic-gelation, natural crosslinking, sol-gel.
  • Antioxidant activities

 

Roles and responsibilities

In charge of assistant engineer internships in biotechnology at University of Technology of Compiègne.

 

General information

  • Since 2006 : Lecturer at Université de Technologie de Compiègne in the Laboratory of Enzyme and Cell Engineering (UMR CNRS 7025)
  • 2005 : Post-doctoral position at Ecole Nationale Supérieure de Chimie de Montpellier, Laboratoire des Matériaux Catalytiques et Catalyse en Chimie Organique, Montpellier, France. “Protein immobilization and encapsulation in nanostructured silica materials for wastewater treatment”.
  • 2004: PhD in Biotechnology and Food Process, at Institut National Polytechnique de Lorraine (INPL, ENSAIA, Nancy, France), PhD thesis on: “Development of an enzymatic process for the production of flavonoid esters : kinetic, purification, conformational study, biological and physic-chemical properties.”
  • 2001: Master in organic chemistry, Paris-Sud XI University. “Bienzymatic system for asymmetric sulfoxidation”.
  • 2000: Engineer degree in organic chemistry, Ecole Nationale Supérieure de Chimie de Rennes (ENSCR), France

 

Articles

Ksouda G., Sellimi S., Merlier F., Facimaigne-cordin A., Thomasset B., Nasri M., Hajji M. Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive . Food Chemistry, 2019, 288,47-56.
     ABSTRACT: The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage. The GC–HRMS analysis of PSEO showed that anethole, pseudoisoeugenol and p-anisaldehyde were the main components. The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential. Additionally, PSEO displayed potent antibacterial activity against 3 Gram-positive and 3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL). The acute toxicity of PSEO was determined using mice model (LD50 = 976.2 mg/kg). The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.

Ksouda G., Hajji M. Sellimi S., Merlier F., Falcimaigne Cordin A., Nasri M., Thomasset B. A Systematic Comparison of 25 Tunisian Plant Species Based on Oil and Phenolic Contents, Fatty Acid Composition and Antioxidant Activity. Ind. Crops Prod, 2018, 274,432-440.
     ABSTRACT: This study investigated 25 Tunisian plant species of 13 families based on their oil and total phenolic contents. The fatty acid profiles and antioxidant activity of phenolic extracts of these plant seeds were studied in order to identify species containing unusual fatty acids and potential antioxidants. The oil content varied from 4.2 to 66.5% of DW (Dry Weight). Fatty acid profiles were determined using gas chromatography analysis coupled to flame ionization detection and high resolution mass spectrometry (GC-FID and GC-HRMS). The results showed that plant seeds were mostly composed of classic fatty acids (palmitic, oleic, linoleic and linolenic acids), while unusual fatty acids such as long-chain and odd-chain fatty acids were minor components. However, the petroselinic acid was the major component of Apiaceae family seed oil, particularly in Pimpinella saxifraga and Pimpinella major. The phenolic content of seed methanolic extracts, measured by Folin

Yarman A., Jetzschmann K. J., Neumann B., Zhang X., Wollenberger U., Cordin A., Haupt K. & Scheller F. W. Enzymes as tools in MIP-sensors.. Chemosensors, 2017, 5,11.
     ABSTRACT: Molecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and are potentially cheaper to produce. However, the affinity and especially the selectivity of MIPs are in general lower than those of their biological pendants. Enzymes are useful tools for the preparation of MIPs for both low and high-molecular weight targets: As a green alternative to the well-established methods of chemical polymerization, enzyme-initiated polymerization has been introduced and the removal of protein templates by proteases has been successfully applied. Furthermore, MIPs have been coupled with enzymes in order to enhance the analytical performance of biomimetic sensors: Enzymes have been used in MIP-sensors as

Attieh M. D., Zhao Y., Elkak A., Falcimaigne-cordin A., & Haupt K. Enzyme-initiated free-radical polymerization of molecularly imprinted polymer nanogels on a solid phase with an immobilized radical source. Angew. Chem. Int. Ed., 2017, 56,3339-3343.
     ABSTRACT: An enzyme?mediated synthetic approach is described for the preparation of molecularly imprinted polymer nanoparticles (MIP?NPs) in aqueous media. Horseradish peroxidase (HRP) was used to initiate the polymerization of methacrylate or vinyl monomers and cross?linkers by catalyzing the generation of free radicals. To prevent entrapment of the enzyme in the cross?linked polymer, and to enable it to be reused, HRP was immobilized on a solid support. MIPs based on 4?vinylpyridine and 1,4?bis(acryloyl)piperazine for the recognition of 2,4?dichlorophenoxyacetic acid (2,4?D) and salicylic acid were synthesized in an aqueous medium. MIPs for the protein trypsin were also synthesized. MIP nanoparticles with sizes between 50 and 300?nm were obtained with good binding properties, a good imprinting effect, and high selectivity for the target molecule. The reusability of immobilized HRP for MIP synthesis was shown for several batches.

Adali-kaya Z., Tse Sum Bui B., Falcimaigne-cordin A. & Haupt K. Molecularly imprinted polymer nanomaterials and nanocomposites: atom-transfer radical polymerization with acidic monomers.. Angew. Chem. Int. Ed., 2015, 54,192-195.
 

Zdunek J., Benito-pena E., Linares A., Falcimaigne-cordin A., Orellana G., Haupt K. & Moreno-bondi M. C. Surface-imprinted nanofilaments for Europium-amplified luminescent detection of fluoroquinolones antibiotics.. Chem.-Eur. J., 2013, 19,10209-10216.
 

Boussetta N., Vorobiev E., Le L. H., Cordin-falcimaigne A. & Lanoiselle J. L. Application of electrical treatments in alcoholic solvent for polyphenols extraction from grape seeds.. LWT-Food Sci. Technol., 2012, 46,127-134.
 

Biffis A., Dvorakova G. & Falcimaigne-cordin A. Physical forms of MIPS.. Top. Curr. Chem., 2012, 325,29-82.
 

Boussetta N., Vorobiev E., Deloison V., Pochez F., Falcimaigne-cordin A. & Lanoiselle J. L. Valorisation of grape pomace by the extraction of phenolic antioxidants: Application of high voltage electrical discharges.. Food Chem., 2011, 128,364-370.
 

Linares A. V., Falcimaigne-cordin A., Gheber L. A. & Haupt K. Patterning nanostructured, synthetic, polymeric receptors by simultaneous projection photolithography, nanomolding, and molecular imprinting.. Small, 2011, 7,2318-2325.
 

Linares A. V., Vandevelde F., Pantigny J., Falcimaigne-cordin A. & Haupt K. Polymer films composed of surface-bound nanofilaments with a high aspect ratio, molecularly imprinted with small molecules and proteins.. Adv. Funct. Mater., 2009, 19,1299-1303.
 

Brigitte THOMASSET

DR CNRS - Co-Animator Theme PMB

        Contact Brigitte Thomasset

 

 

Articles

Imatoukene N., Back A., Nonus M.,  thomasset B., Rossignol T. Et Nicaud J. M. Fermentation process for producing CFAs using Yarrowia lipolytica . Journal of Industrial Microbiology and Biotechnology , 2020, 47 ,403-412 .
 

Martins-noguerol R., Moreno-perez A. J., Acket S., Troncoso-ponce A., Garces R., Thomasset B., Salas J. J. Et Martinez Force E. Impact of sunflower (Helianthus annuusL.) plastidial lipoyl synthases genes expression in glycerolipids of transgenic Arabidopsis plants . Scientific Reports , 2020, 10 ,3749-3764 .
 

Acket S., Degournay A., Rossez Y., Mottelet S., Villon P., Troncoso-ponce A., Thomasset B. 13C-Metabolic Flux Analysis in Developing Flax (Linum usitatissinum L.) Embryos to Understand Storage Lipid Biosynthesis . Metabolites , 2020, 10,14.
 

Fahs Z., Rossez Y., Guénin S., Gutierrez L., Thomasset B., & Perrin, Y. Cloning and molecular characterization of three lysophosphatidic acid acyltransferases expressed in flax seeds. Plant Science, 2019, 280,41-50.
 

Merlier F., Octave S., Tse Sum Bui B., Thomasset B. Evaluation of Performance and Validity Limits of Gas Chromatography electron ionization – Orbitrap Detector for fatty acid methyl esters analyses . Rapid Commun Mass Spectrom, 2019, in press,.
 

Martins-noguerol R., Moreno-pérez Aj., Acket S., Makni S., Garcés R., Troncoso-ponce A., Salas Jj., Thomasset B., Martínez-force E. Lipidomic Analysis of Plastidial Octanoyltransferase Mutants of Arabidopsis thaliana . Metabolites , 2019, 9(10) ,209 .
 

Czerwic Q., Idrissitaghki A., Imatoukene N., Nonus M., Thomasset B., Nicaud J-m., Rossignol T. Optimization of cyclopropane fatty acids production in Yarrowia lipolytica. Yeast, 2019, 36,143-151.
     ABSTRACT:  Cyclopropane fatty acids, which can be simply converted to methylated fatty acids, are good unusual fatty acid candidates for long‐term resistance to oxidization and low‐temperature fluidity useful for oleochemistry and biofuels. Cyclopropane fatty acids are present in low amounts in plants or bacteria. In order to develop a process for large‐scale biolipid production, we expressed 10 cyclopropane fatty acid synthases from various organisms in the oleaginous yeast Yarrowia lipolytica, a model yeast for lipid metabolism and naturally capable of producing large amounts of lipids. The Escherichia coli cyclopropane fatty acid synthase expression in Y. lipolytica allows the production of two classes of cyclopropane fatty acids, a C17:0 cyclopropanated form and a C19:0 cyclopropanated form, whereas others produce only the C17:0 form. Expression optimization and fed‐batch fermentation set‐up enable us to reach a specific productivity of 0.032 g·L−1·hr−1 with a genetically modified strain containing cyclopropane fatty acid up to 45% of the total lipid content corresponding to a titre of 2.3 ± 0.2 g/L and a yield of 56.2 ± 4.4 mg/g.

Ksouda G., Sellimi S., Merlier F., Facimaigne-cordin A., Thomasset B., Nasri M., Hajji M. Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive . Food Chemistry, 2019, 288,47-56.
     ABSTRACT: The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage. The GC–HRMS analysis of PSEO showed that anethole, pseudoisoeugenol and p-anisaldehyde were the main components. The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential. Additionally, PSEO displayed potent antibacterial activity against 3 Gram-positive and 3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL). The acute toxicity of PSEO was determined using mice model (LD50 = 976.2 mg/kg). The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.

Tshabuse F., Farrant J. M., Humbert L., Moura D., Rainteau D., Espinasse C., Idrissi A., Merlier F., Acket S., Rafudeen M. S., Thomasset B. & Ruelland E. Glycerolipid analysis during desication and recovery of the resurrection plant Xerophyta humilis (Bak) Dur and Schinz.. Plant Cell Environ., 2018, 41,533-547.
     ABSTRACT: Feelings in humans are mental states representing groups of physiological functions that usually have defined behavioural purposes. Feelings, being evolutionarily ancient, are thought to be coordinated in the brain stem of animals. One function of the brain is to prioritise between competing mental states and, thus, groups of physiological functions and in turn behaviour. Plants use groups of coordinated physiological activities to deal with defined environmental situations but currently have no known mental state to prioritise any order of response. Plants do have a nervous system based on action potentials transmitted along phloem conduits but which in addition, through anastomoses and other cross?links, forms a complex network. The emergent potential for this excitable network to form a mental state is unknown, but it might be used to distinguish between different and even contradictory signals to the individual plant and thus determine a priority of response. This plant nervous system stretches throughout the whole plant providing the potential for assessment in all parts and commensurate with its self?organising, phenotypically plastic behaviour. Plasticity may, in turn, depend heavily on the instructive capabilities of local bioelectric fields enabling both a degree of behavioural independence but influenced by the condition of the whole plant.

Merlier F., Imatoukene N., Octave S., Nicaud J. M., Thomasset B. A Gas Chromatography Full Scan High Resolution Orbitrap Mass Spectrometry Method for Separation and Characterization of 3-Hydroxymethyl Pyridine Ester of Fatty Acids at Low Levels. J. Chromatogr, 2018, 123,768-778.
     ABSTRACT: Fatty acid methyl esters (FAMEs), which are commonly used to characterize lipids, have several limitations to conclude on many structures. 3-Pyridylcarbinol esters (3-PCE) are used to characterize fatty acid structures [1], in particular, to identify ring and double bond positions on the carbon chain. Chromatographic separation of these esters is complex due to their polarity and high boiling points. In this study, we used a column with high resolutive power based on ionic liquids to increase the separation quality in gas chromatography (GC). In addition, we used a high-resolution detector (Orbitrap) to limit non-specific signals and improve the detection limits. This detector could be used with a mass filter at 5?ppm for the rapid determination of 3-PCE from its characteristic ions (m/z?=?108.0441 and 92.0495). This filter allowed the identification of derivative fatty acids with good sensibility. Thus, it was possible to characterize 3-PCE by measuring the exact fragment masses to confirm structures such as C19:2n12cyclo?9.

Ksouda G., Hajji M. Sellimi S., Merlier F., Falcimaigne Cordin A., Nasri M., Thomasset B. A Systematic Comparison of 25 Tunisian Plant Species Based on Oil and Phenolic Contents, Fatty Acid Composition and Antioxidant Activity. Ind. Crops Prod, 2018, 274,432-440.
     ABSTRACT: This study investigated 25 Tunisian plant species of 13 families based on their oil and total phenolic contents. The fatty acid profiles and antioxidant activity of phenolic extracts of these plant seeds were studied in order to identify species containing unusual fatty acids and potential antioxidants. The oil content varied from 4.2 to 66.5% of DW (Dry Weight). Fatty acid profiles were determined using gas chromatography analysis coupled to flame ionization detection and high resolution mass spectrometry (GC-FID and GC-HRMS). The results showed that plant seeds were mostly composed of classic fatty acids (palmitic, oleic, linoleic and linolenic acids), while unusual fatty acids such as long-chain and odd-chain fatty acids were minor components. However, the petroselinic acid was the major component of Apiaceae family seed oil, particularly in Pimpinella saxifraga and Pimpinella major. The phenolic content of seed methanolic extracts, measured by Folin

Acket S., Degournay A., Gosset M., Merlier F., Troncoso-ponce M. A. & Thomasset B. Analysis of C-13 labeling amino acids by capillary electrophoresis - High resolution mass spectrometry in developing flaxseed.. Anal. Biochem., 2018, 547,14-18.
     ABSTRACT: n context of fluxomic studies, 13C labeling analysis of amino acids are very important for solving the carbon flow calculation, because they are synthesized in various biosynthesis pathways and cellular compartments in plant cells. Traditionally, 13C labeling analysis are performed using low resolution mass spectrometry detector by GC-MS. We compared a method using capillary electrophoresis-high resolution mass spectrometry without derivatization and with better accuracy assessment of labeling measurements comparing to classical GC-MS. Our method allowed us to show that valine, leucine, alanine are not synthesized from the same pyruvate pool during the period of reserves accumulation in flax seeds.

Hano C., Corbin C., Drouet S., Quero A., Rombaut N., Savoire R., MoliniÉ R., Thomasset B., Mesnard F., LainÉ E. The lignan (+)-secoisolariciresinol extracted from flax hulls is an effective protectant of linseed oil and its emulsion against oxidative damage . Eur. J. Lipid Sci. Technol. , 2017, 119,1600219.
     ABSTRACT: Secoisolariciresinol (SECO) is a natural antioxidant lignan accumulated in large amounts in the seedcoat of flax and retained in the flaxseed cake residue during linseed oil pressing. Here SECO was extracted and purified from flaxseed cake and assayed for its ability to prevent oxidation of linseed oil and an o/w emulsion containing linseed oil. For this purpose, an accelerated storage (Schaal oven) test was performed and SECO effectiveness was compared to that of two antioxidants commonly used in food and cosmetic products: ??tocopherol (??TOCO) and butylated hydroxyanisole (BHA). In our hands, SECO addition, ranging from 50 to 500??mole per kg oil, significantly decreased the production of both primary (conjugated dienes, CD) and secondary (thiobarbituric acid?reactive substances, TBARS) oxidation products. This study evidenced that SECO is an effective stabilizer of linseed oil and its o/w emulsion and this protective effect outperformed both the natural ??TOCO and the synthetic BHA antioxidants. In particular, SECO was the most effective in the protection of the o/w emulsion against secondary oxidation products, which makes it a potential alternative preservative for oily products in foods and cosmetics.

Acket S., Degournay A., Merlier F. & Thomasset B. 13C labeling analysis of sugars by hight resolution-mass spectrometry for metabolic flux analysis.. Anal. Biochem., 2017, 527,45-48.
     ABSTRACT: Metabolic flux analysis is particularly complex in plant cells because of highly compartmented metabolism. Analysis of free sugars is interesting because it provides data to define fluxes around hexose, pentose, and triose phosphate pools in different compartment. In this work, we present a method to analyze the isotopomer distribution of free sugars labeled with carbon 13 using a liquid chromatography

Hano C., Corbin C., Quero A., Rombaut N., Savoire R., Molinie R., Thomasset B., Mesnard F. & Laine E. The use of (+)-secoisolariciresinol extracted from the hulles as en effective protectant of linseed oil and its emulsion against oxidative damage. . Eur. J. Lipid Sci. Technol, 2017, 118,
  

Rombaut N., Savoire R., Van Hecke E. Et Thomasset B. Supercritical CO2 extraction of linseed: Optimization by experimental design with regards to oil yield and composition. Eur. J. Lipid Sci. Technol, 2017, 119,1600078.
     ABSTRACT: The aim of the present study is to evaluate the effect of CO2 pressure, temperature, and CO2 flow rate on supercritical fluid extraction of linseed oil using neat CO2. Chosen methodology is based on central composite rotatable design, responses studied are extract yield, oil yield, water?extract ratio, oil ??tocopherol content, oil polyphenols content, alpha linolenic acid proportion in oil, and oil acid value. Acid value, tocopherol content, and linolenic acid proportion are independent responses of studied factors. On other responses, only pressure and temperature have an influence. Based on response surface analysis and multiresponses optimization, optimal conditions for oil yield, and oil polyphenol content maximization and water?extract minimization were determined (42.5?MPa, 120

Imatoukenen N., Verbek J., Beopoulos A., Idrissi Taghki A., Thomasset B., Sarde C. O., Nonus M. & Nicaud J. M. Metabolic engineering for Conjugated Linoleic Acid production in the oleaginous yeast in Yarrowia lipolytica.. Applied Microbiol. Biotechnol, 2017, 101,4605-4616.
     ABSTRACT: Conjugated linoleic acids (CLAs) have been found to have beneficial effects on human health when used as dietary supplements. However, their availability is limited because pure, chemistry-based production is expensive, and biology-based fermentation methods can only create small quantities. In an effort to enhance microbial production of CLAs, four genetically modified strains of the oleaginous yeast Yarrowia lipolytica were generated. These mutants presented various genetic modifications, including the elimination of ?-oxidation (pox1-6

Acket S., Degournay A., Merlier F. & Thomasset B. Data documenting the comparison between the theoretically expected values of free sugars mass isotopomer composition with standards using GC-MS and LC-HRMS for Metabolic Flux Analysis.. Data in Brief, 2017, 12,108-112.
     ABSTRACT: The data presented in this article are related to the research article entitled

Quero A., Molinie R., Mathiron D., Thiombiano B., Fontaine J. F., Brancourt D., Van Wuytswinkel O., Petit E., Demailly H., Mongelard G., Pilard S., Thomasset B. & Mesnard F. Metabolite profiling in developing Camelina sativa seeds.. Metabolomics, 2016, 12,186-199.
 

Rombaut N., Savoire R., Thomasset B., Castello J. & Van Hecke E. Optimization of oil yield and oil total phenolic content during grape seed cold screw pressing.. Ind. Crops Prod., 2015, 63,26-33.
 

Lazouk M. A., Savoire R., Kaddour A., Castello J., Lanoiselle J. L., Van Hecke E. & Thomasset B. Oilseeds sorption isoterms, mechanical properties and pressing.: Global view of water impact.. J. Food Eng., 2015, 153,73-80.
 

Savoire R., Lazouk M. A., Van-hecke E., Roulard R., Tavernier R., Guillot X., Rhazi L., Petit E., Mesnard F. & Thomasset B. Environmental and varietal impact on linseed composition and on oil unidirectional expression process.. OCL, 2015, 22,D605.
 

Rombaut N., Savoire R., Thomasset B., Belliard T., Castello J., Van Hecke E. & Lanoiselle J. L. Grape seed oil extraction: interest of supercritical fluid extraction and gas assisted mechanical extraction for enhancing polyphenol co-extraction in oil.. C.R. Chimie, 2014, 17,284-292.
 

Koubaa M., Thomasset B. & Roscher A. Quantifying 13C-label in free sugars and starch by GC-MS.. Methods Molec. Biol., 2014, 1090,121-130.
 

Fenart S., Chabi M., Gallina S., Huis R., Neutelings G., Riviere N., Thomasset B., Hawkins S. & Lucau-danila A. Intra-platform comparison of 25-mer and 60-mer oligonucleotide Nimblegen DNA microarrays.. BMC Res. Notes, 2013, 6,43.
 

Chantreau M., Grec S., Gutierrez L., Dalmais M., Pineau C., Demailly H., Paysant-leroux C., Tavernier R., Trouve J. P., Chatterjee M., Guillot X., Brunaud V., Chabbert B., Van Wuytswinkel O., Bendahmane A., Thomasset B. & Hawkins S. PT-Flax (phenotyping and TILLing of flax): development of a flax (Linum usitatissimum L.) mutant population and TILLing platform for forward and reverse genetics.. BMC Plant Biol., 2013, 13,159-174.
 

Koubaa M., Cocuron J. C., Thomasset B. & Alonso A. Highlighting the tricarboxylic acid cycle : liquid and gas chromatography-mass spectrometry analyses of 13C-labeled organic acids.. Anal. Biochem., 2013, 436,151-159.
 

Koubaa M., Mghaieth S., Thomasset B. & Roscher A. Gas chromatography-mass spectrometry analysis of 13C labeling in sugars for metabolic flux analysis.. Anal. Biochem., 2012, 425,183-188.
 

Pilalis E., Chatziioannou A., Thomasset B. & Kolisis F. An In-silico compartmentalized, metabolic model of Brassica napus, enables the systemic study of regulatory aspects of plant central metabolism.. Biotechnol. Bioeng., 2011, 108,1673-1682.
 

Fenart S., Ndong Y. P., Duarte J., Riviere N., Van Wuytswinkel O., Lucau A., Cariou E., Neutelings G., Gutierrez L., Chabbert B., Guillot X., Tavernier R., Hawkins S. & Thomasset B. Development and validation of a flax (Linum usitatissinum L.) gene expression oligo microarray.. BMC Genomics, 2010, 11,592.
 

Jousse C., Schiltz S., Fournez A., Guillot X., Thomasset B., Gougeon S., Bourgaud F. & Gontier E. Rapid, cost-effective screening of flax genotypes to identify desirable fatty acid compositions.. Electron. J. Plant Breed., 2010, 1,1396-1404
  

Nlandu Mputu M., Rhazi L., Vasseur G., Vu T. D., Gontier E. & Thomasset B. Identification of a potential bottleneck in branched chain fatty acid incorporation into triacylglycerol for lipid biosynthesis in agronomic plants.. Biochimie, 2009, 91,703-710.
 

Lievre K., Tran T. L., Doerper S., Hehn A., Lacoste P., Thomasset B., Bourgaud F. & Gontier E. Agrobacterium-mediated transformation of Ruta graveciens L.. Methods Molec. Biol., 2009, 547,235-248.
 

Caillot S., Rosiau E., Laplace C. & Thomasset B. Influence of light intensity and selection scheme on regeneration time of transgenic flax plants.. Plant Cell Rep., 2009, 28,359-371.
 

Noizet M., Harrabi F., Vijayalakshmi M., Galbraith D., Thomas D. & Thomasset B. Targeted protein accumulation promoted by autoassembly and its recovery from plan cells.. Biotechnol. J., 2008, 3,392-402.
 

Troufflard S., Roscher A., Thomasset B., Barbotin J. N., Rawsthorne S. & Portais J. C. In vivo (13)C NMR determines metabolic fluxes and steady state in linseed embryos.. Phytochem., 2007, 68,, 2341-2350.
 

Gutierrez L., Conejero G., Castelain M., GuÉnin S., Verdeil J. L., Thomasset B. & Van Wuytswinkel O. Identification of new gene expression regulators specifically expressed during plant seed maturation.. J. Exp. Bot., 2006, 57,1919-1932.
 

Patent

P. Carre, A. Lazouk, B. Thomasset, R. Savoir, L. Rhazi. Procédé de décorticage de graines. , 2015, ,.
 

Sonia RIPPA

Co-animator theme PMB - Competent Person in Radiation Protection - Manager of L2 & S2

             Contact Sonia Rippa

 

 

Research

I am involved in the “Plant Metabolism and Bioresources” theme to study the carnitine biosynthesis pathway in Arabidopsis and get insights in the carnitine physiological roles in plants, especially in lipid metabolism and stress tolerance.

I collaborate with the “Biomimicry and Biomolecular Diversity” theme in a transversal approach to study interactions of natural amphiphilic compounds with biomimetic membranes and understand the mode of action of such molecules to trigger plant defenses or to inhibit phytopathogens. I study the physiological effects of the compounds, the defense pathways and mechanisms induced in plants (Arabidopsis, rapeseed) and microorganisms.

I lead a more applicative project aiming to optimize and validate the efficiency of rhamnolipids to protect rapeseed from pathogenic fungi in the field.

I look for new biocontrol agents or new biocontrol strategies.

 

Skills 

Scientific skills: Plant physiology, plant defense, plant stress tolerance, phytopathogens, antimicrobial control, biocontrol

Technical skills: Molecular biology, transcriptomic, biochemistry, plant mutants, microbiology, microscopy, mass spectrometry

 

Roles and responsabilities

  • Person Competent in Radiation protection (Nuclear Safety Authority correspondent)
  • Biosafety Level 2 Laboratories Manager (human cells, bacteria, plants)
  • GMO correspondent
  • Teaching: In charge of the Biocontrol BG08 UE (M2 degree, Master Chimie, parcours biotechnologie UTC UPJV)

 

General information

My first academic research experience took place in 1998 in the Plant Science Institute (CNRS, Gif-sur-Yvette). I got molecular biology skills in the Dr A. Kondorosi’s laboratory, with Dr P. Ratet in a Medicago truncatula T-DNA tagging project. I then joined the group of Dr J. Giraudat to work with Dr F. Parcy to study the fine-tuning gene expression process during late embryogenesis in Arabidopsis. In 2001, I got an Engineer position in the University of Technology of Compiègne in the Enzyme and Cell Engineering Unit. In 2003, I started a PhD to study the interaction and the defense mechanisms induced by peptaibols (amphiphilic peptides mainly produced by Trichoderma fungi) on the plant Arabidopsis. In 2007, I got involved in a project aiming to study the carnitine role in plants. This project was initiated by Pr Y. Perrin in the PMB theme. I more precisely studied the carnitine biosynthesis pathway in Arabidopsis to identify enzymes involved in carnitine synthesis and to study mutants of these enzymes. Since 2014, as a Research Engineer, I have initiated a project with Pr C. Sarazin, to study the mode of perception/action of natural amphiphilic glycolipids by plants or against phytopathogens.

 

Personal interests

Reading, travelling, French gastronomy…

 

Main publications

Articles

Monnier N., Furlan A-l., Buchoux S., Deleu M., Dauchez M., Rippa S., Sarazin C. Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies" has been published in IJMS as part of the Special Issue SAR "Out of the Box. Int. J. Mol. Sci., 2019, 20,1009.
 

Furlan A., Laurin Y., Botcazon C., Rodríguez-moraga N., Rippa S., Deleu M., Lins L., Sarazin C., Buchoux s. Semi-purified rhamnolipid mixes protect Brassica napus against Leptosphaeria maculans early infections . Phytopathology , 2019, 110(4) ,834-842 .
 

Monnier M., Furlan A., Botcazon C., Dahi A., Mongelard G., Cordelier S., Clement C., Dorey S., Sarazin C., Rippa S. Rhamnolipids from Pseudomonas aeruginosa are elicitors triggering Brassica napus protection against Botrytis cinerea without physiological disorders. Frontiers in Plant Science, 2018, 1778,101-124.
     ABSTRACT: Rhamnolipids (RLs) are amphiphilic molecules naturally produced by some bacteria with a large range of biological activities. Although some studies report their potential interest in plant protection, evaluation of their effects and efficiency on annual crops of worldwide agronomic interest is lacking. The main objective of this work was to investigate their elicitor and protective activities on rapeseed crop species while evaluating their physiological effects. Here we report that RLs from Pseudomonas aeruginosa secretome trigger an effective protection of Brassica napus foliar tissues toward the fungus Botrytis cinerea involving the combination of plant defense activation and direct antimicrobial properties. We demonstrated their ability to activate canonical B. napus defense responses including reactive oxygen species production, expression of defense genes, along with callose deposits and stomatal closure as efficient physical protections. In addition, microscopic cell death observations and electrolyte leakage measurements indicated that RLs trigger a hypersensitive response-like defense in this plant. We also showed that foliar spray applications of RLs do not induce deleterious physiological consequences on plant growth or chlorophyll content and that RL protective properties are efficient on several grown cultivars of rapeseed. To our knowledge, this is the first report of RLs as an elicitor that suppresses fungal disease on tissues of an annual crop species under greenhouse conditions. Our results highlight the dual mode of action of these molecules exhibiting plant protection activation and antifungal activities and demonstrate their potential for crop cultures as environmental-friendly biocontrol solution.

Jacques F., Rippa S., Perrin Y. Physiology of L-carnitine in plants in light of the knowledge in animals and microorganisms. Plant Science, 2018, 9,1170.
     ABSTRACT: L-carnitine is present in all living kingdoms where it acts in diverse physiological processes. It is involved in lipid metabolism in animals and yeasts, notably as an essential cofactor of fatty acid intracellular trafficking. Its physiological significance is poorly understood in plants, but L-carnitine may be linked to fatty acid metabolism among other roles. Indeed, carnitine transferases activities and acylcarnitines are measured in plant tissues. Current knowledge of fatty acid trafficking in plants rules out acylcarnitines as intermediates of the peroxisomal and mitochondrial fatty acid metabolism, unlike in animals and yeasts. Instead, acylcarnitines could be involved in plastidial exportation of de novo fatty acid, or importation of fatty acids into the ER, for synthesis of specific glycerolipids. L-carnitine also contributes to cellular maintenance though antioxidant and osmolyte properties in animals and microbes. Recent data indicate similar features in plants, together with modulation of signaling pathways. The biosynthesis of L-carnitine in the plant cell shares similar precursors as in the animal and yeast cells. The elucidation of the biosynthesis pathway of L-carnitine, and the identification of the enzymes involved, is today essential to progress further in the comprehension of its biological significance in plants.

Nguyen P. J., Rippa S., Rossez Y. & Perriny. Acylcarnitines participate in developmental processes associated to lipid metabolism in plants.. Planta, 2016, 243,1011-1022.
 

Rippa S., Zhao Y., Merlier F., Charrier A. & Perrin Y. The carnitine biosynthetic pathway in Arabidopsis thaliana shares similar features with the pathway of mamals and fungi.. Plant Physiol. Biochem., 2012, 60,109-114.
 

Charrier A., Rippa S., Yu A., Nguyen P. J., Renou J. P. & Perrin Y. The effect of carnitine on Arabidopsis development and recovery in salt stress conditions.. Planta, 2012, 235,123-135.
 

Rippa S., Eid M., Formaggio F., Toniolo C & Beven L. Hypersensitive-like response to the pore-former peptaibol alamethicin in Arabidopsis thaliana. Chembiochem, 2010, 11,2042-2049.
 

Eid M., Rippa S., Castano S., Desbat B., Chopineau J. Rossi C. & Beven L. Exploring the membrane mechanism of the bioactive peptaibol ampullosporin A using lipid monolayers and supported biomimetic membranes. J. Biophys., 2010, 2010,Art.ID 179641.
 

Rippa S, Adenier H, Derbaly M. And Beven L. The peptaibol alamethicin induces an rRNA-cleavage-associated death in Arabidopsis thaliana.. Chem. Biodivers., 2007, 4,1360-1373.
 

Valérie SANTONI

Logistics - Management Chemical Products Database

           Contact Valérie SANTONI

 

Research

Involved in research projects of the theme “Plant Metabolism and Bioresources”, namely with Sonia Rippa on “Inhibiting effect of compounds of mycelial growth of phytopathogenic fungi” and Yolande Perrin on “Biotechnology of microalgae”.

Skills

Plant cultures, DNA extraction, microbiological and biological assays. Financial management and collective logistics. SIFAC / CNRS

Virginie LAMBERTYN

Manager Analytical Platform - Safety Assistant UPJV/CNRS

Research
Involved in research projects in the “Plant metabolism and bioresources” theme, more particularly in the valorization of the three main polymers constituting lignocellulosic biomass (wheat, miscanthus, horse manure)

Skills
Chemical, microbiological analysis techniques and biomolecule analyses.
Health and safety regulations
Professional risks and their prevention
Professional environment and networks

Roles and responsibilities

  • Technician in chemistry and physical sciences: Carries out laboratory experiments in the fields of chemical analysis, biochemical and microbiological synthesis, on various projects. And development of new analysis or synthesis techniques for the identification and quantification of molecules.
  • Management of the analytical equipment: High Performance Liquid Chromatography (RI, UV, diode strip, DEDL), ion exchanger (PAD) and steric exclusion (SEC), pH meter, conductivity meter, UV spectrometer -visible and DLS, refractometer, rotary evaporators, lyophilizer, titrimeter, centrifuge, autoclave, bioreactor.
  • UPJV/CNRS prevention assistant: Established the single document in collaboration with the unit director, using the software: Professional Risk Evaluation.
  • Raise awareness and inform staff, taking into account the specific risks linked to our work environment. CNRS NEO training for all newcomers. Ensures compliance with safety rules (GLP).
  • Manages the storage and disposal of products using the Quartzy database.
  • Manages the supply of consumables, as well as equipment (quote request, operational and investment purchases) and realization of fixed assets.
  • Follow budgets, carry out orders and services made on SIFAC
  • Workplace First Aider (SST)

Main publications
Articles

  • DOI:10.1016/j.indcrop.2023.116627
    M. Quesada-Salas, M E. Vuillemin, J. Dillies, R. Dauwe, L. Firdaous, M. Bigan, V. Lambertyn, D. Cailleu, A. Jamali, R. Froidevaux, E. Husson, C.Sarazin
    1-ethyl-3-methyl imidazolium acetate, hemicellulolytic enzymes and laccase-mediator system: Toward an integrated co-valorization of polysaccharides and lignin from Miscanthus.
  • DOI : 10.1016/j.procbio.2022.03.015
    M E. Vuillemin, E. Husson, S. Laclef, A. Jamali, V. Lambertyn, S. Pilard, D. Cailleu, C. Sarazin.
    Improving the environmental compatibility of enzymatic synthesis of sugar-based surfactants using green reaction media .
  • DOI: 10.1016/j.carbpol.2019.115382
    G. Huet, C. Hadad, E. Husson, S. Laclef, V. Lambertyn, M. ArayaFarias, A. Jamali, M. Courty, R. Alayoubi, I. Gosselin, C. Sarazin, A. Nguyen Van Nhien
    Straightforward extraction and selective bioconversion of high purity chitin from Bombyx eri larva: Toward an integrated insect biorefinery.
  • DOI : 10.3389/fchem.2019.00585
    M. Araya-Farias, E. Husson, D. Gérard, V. Lambertyn, J. Saavedra, I. Gosselin, R. Roulard, C. Rémond, C. Sarazin.
    Wheat Bran Pretreatment by Room Temperature Ionic Liquid-Water Mixture: Optimization of Process Conditions by PLS-Surface Response Design.
  • DOI: 10.1039/C8NJ03237H
    A L. Furlan, S. Buchoux, Y. Miao, V. Banchet, M. Létévé, V. Lambertyn, J. Michel, C. Sarazin, V. Bonnet.
    Nanoparticles based on lipidyl-β-cyclodextrins: synthesis, characterization, and experimental and computational biophysical studies for encapsulation of atazanavir.
  • DOI:10.1016/j.biortech.2017.12.047
    E. Hussona, T. Aux enfans, M. Herbaut, M. Baralle, V. Lambertyn, H. Rakotoarivonina, C. Rémond, C. Sarazin
    Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases.
  • DOI : 10.1039/C7GC01471F
    E. Husson, C. Hadad, G. Huet, S. Laclef, D. Lesur, V. Lambertyn, A. Jamali, S. Gottis, C. Sarazin and A. Nguyen Van Nhien
    Effect of room temperature ionic liquids on selective biocatalytic hydrolysis of chitin via sequential or simultaneous strategies.

 

Contact Virginie Lambertyn

Valérie ROBQUIN

Logistics - Management Chemical Products Database

          Contact Valérie Robquin

 

Research

Involved in research projects of the theme “Plant Metabolism and Bioresources” and, in particular, the transverse project “study of the interaction of bacterial flagella and biotic and abiotic surfaces” with Yannick Rossez.
Skills

Plant cultures, extraction of molecules, biological and microbiological assays. Financial management and collective logistics. SIFAC / CNRS
Roles and responsabilities

Technician in Biology. Order forms registration in SIFAC software as well as monitoring and distribution of biological and chemical product ordered. Unit consumables management (bunker, alcohols, laboratory consumables, office supplies). Registration of product entries and exits in the laboratory’s products database (GPUC). Tracking orders and relaunching suppliers in case of delay or undelivery.
General information

  • 1989: Preparator in Pharmacy BP obtained
  • Since 11/1989: Technician in GEC unit
  • Treasurer of L’Amicale of UTC

 

Personal interests
Cooking, decoration

Pascal BOULNOIS

Informatics Correspondent - Maintenance

        Contact Pascal Boulnois

Elise PROST

Training Correspondent - Manager RMN/RAMAN/FTIR Platform

              Contact Elise Prost

 

 

Research

Member of the theme “Biomimicry and Biomolecular Diversity”. I’m CNRS engineer in the Enzymatic and Cell Engineering (GEC) laboratory and collaborate directly with Luminita Duma (CR CNRS) within the MIP team. I’m involved in the various projects of the unit that require NMR, Raman or InfraRed skills.

 

Roles and responsabilities

  • In charge of the various devices: RMN Bruker Avance III 400, Raman Witec Alpha 300S and IR Thermo IS50
  • ITRF Jury Expert
  • Laboratory Training Correspondent
  • ITRF/ITA representative on the unit council

 

General information

I’m chemist specialized in NMR thanks to my degree in physicochemical analysis and an internship on the NMR of oligosaccharides. My experiences in different laboratories have confirmed this expertise. I am since 2013, assigned to the GEC unit, as a CNRS engineer in charge of NMR, Raman and InfraRed devices.

  • Since april 2013:IE CNRS – University of Technology of Compiègne. In charge of the plateforms NMR, Raman et IR
  • 2006 – 2013: IE CNRS – University Paris Descartes. In charge of the NMR magnets Bruker Avance 300 et 400, Organic structural analysis
  • 2001 – 2005: IE CNRS – University of Reims. In charge of the NMR magnets. NMR methods development / Organic structural analysis (NMR and molecular modeling) Training
  • 2000: Professional Master “Instrumentation and Physico-Chemical Methods of Analysis”, option: organic analysis, UFR Sciences – Orsay
  • 1999: Master “Organic Chemistry”, UFR Sciences – Orsay
  • 1998: “Maîtrise” degree in chemistry, option: “analytical techniques in molecular chemistry”, UFR Sciences – Dijon

 

Main Publications

Articles

Xu J., Prost E., Haupt K. & Tse Sum Bui B. Direct and sensitive determination of trypsin in human urine using a water-soluble signaling fluorescent molecularly imprinted polymer nanoprobe. . Sensor. Actuat. B-Chem. , 2018, 258,10-17.
     ABSTRACT: https://doi.org/10.1016/j.snb.2017.11.077

Mourao C. A., Bokeloh F., Xu J., Prost E., Duma L., Merlier F., Bueno S. M. A., Haupt K. & Tse Sum Bui, B. Dual oriented solid phase molecular imprinting: Toward selective artificial receptors for recognition of nucleotides in water. Macromolecules, 2017, 50,7484-7490.
     ABSTRACT: We describe the synthesis of water-soluble molecularly imprinted polymer nanoparticles (MIP-NPs) as a new artificial host receptor for the recognition of adenosine monophosphate (AMP), used herein, as a model nucleotide. MIP-NPs were prepared by solid-phase synthesis on glass beads (GB) using, for the first time, immobilized Fe(III)-chelate as an affinity ligand to orientate the AMP via its phosphate group. A polymerizable thymine monomer which can induce complementary base-pairing with the adenine moiety of the nucleotide was synthesized and incorporated in the polymerization mixture to constrain the AMP in a dual-orientated configuration. The MIP-NPs were remarkably selective toward AMP as they did not bind other nucleotides, GMP, UMP, and CMP. This strategy of using the phosphate group of AMP as a hinge enables unhindered pairing of the nucleobase with its corresponding complementary base monomer and can be extended to the preparation of specific MIP receptors for other key nucleotides in aqueous conditions.

Nestora S., Merlier F., Prost E., Haupt K., Rossi C. & Tse Sum Bui B. Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer.. J. Chromatogr. A, 2016, 1465,47-54.
 

Panagiotopoulou M., Salinas Y., Beyazit S., Kunath S., Mayes A. G., Duma L., Prost E., Resmini M., Tse Sum Bui B. & Haupt K. Molecularly imprinted polymer-coated quantum dots for multiplexed cell targeting and imaging.. Angew. Chem. Int. Ed., 2016, 55,8244-8248.
 

Nestora S., Merlier F., Beyazit S., Prost E., Duma L., Baril B., Graves A., Haupt K. & Tse Sum Bui B. Plastic antibodies for cosmetics: Molecularly imprinted polymers scavenge precursors of malodors. . Angew. Chem. Int. Ed., 2016, 55,6252-6256.
 

Nowacki L., Vigneron P., Rotellini L., Cazzola H., Merlier F., Prost E., Ralanairina R., Gadonna J. P., Rossi C. & Vayssade M. Betanin-enriched red beetroot (Beta vulgaris L.) extract induces apoptosis and autophagic cell death in MCF-7 cells.. Phytother. Res., 2015, 29,1964-1973.
 

Boultadakis-arapinis M., Gandon V., Prost E., Micouin L. & Lecourt T. Electronic effects in carbene-mediated C-H bond functionalization: an experimental and theoretical study.. Adv. Synth. Catal., 2014, 356,2493-2505.
 

Laëtitia NUNCQ

Secretary UPJV - Finances

            Contact Laetitia Nuncq

 

Skills

  • Secretary: physical and telephone reception, mailing, meeting programming, reception of newcomers to the lab (various forms, rules of procedure …)
  • Management: orders registration (purchase order, …), inventory of supplies
    Assistance with budget management: SIFAC consultation, contacts with financial and research services
  • Travel processing: Online travel form request and reimbursement tracking for lab members in Amiens and of invited external members

 

Roles and responsabilities

Secretary of part of GEC laboratory located in Amiens, Jules Verne University
General information

  • June 1999: Certificate of Professional Secretary Studies
  • June 2001: Professional Bachelor of Secretary

 

Personal interests

Music, cinema

Morgane LUPPI

Secretary UTC - Finances

         Contact Morgane Luppi

 

 

Skills
Administrative and financial management
Roles and responsabilities

Secretary GEC, 3rd cycle, Master 2 Chemistry; assistance to the financial management of GEC unit

Financial management assistance of GEC unit:

  • registration and follow up of orders
  • suppliers relaunching
  • bills tracking
  • budget administration
  • assistance to the financial reporting of research projects

Secretary of GEC unit and M2:

  • welcoming of new entrants
  • administrative registration of new entrants
  • set up and follow up of all administrative procedures with UTC
  • management of schedules, shared agenda, holidays
  • mail management
  • entry of meeting minutes
  • organization and administrative registration of collaborative/meeting travels
  • expenses reports

 

General information

  • Since 2014: Secretary and management assistant – GEC Laboratory, University of Technology of Compiègne
  • 2012-2014: Brevet of senior technician, assistant of management of SME-SMI – Lycée Jean Calvin in Noyon – Laboratory GEC, University of Technology of Compiègne
  • 2009-2012: Bachelor’s degree, Secretary – Lycée Mireille Grenet in Compiègne

Carol SCHEMBRI

Administration, Finances, Communication - Safety Assistant UTC/CNRS

           Contact Carol Schembri

 

Skills

  • Research projects: budget preparation and monitoring, financial management, financial and administrative reporting
  • Implementation and control of management procedures
  • Recruitment and personnel management
  • Risk Management, Training Students and Newcomers

 

Roles and responsabilities

  • Administrative manager of the Enzymatic and Cell Engineering unit, UMR 7025 CNRS
  • Correspondent Human Resources
  • Correspondent Communication
  • Trained in Safety techniques
  • Member of the Technical Committee at UTC
  • Member of CHSCT at UTC

 

General information

Since 2014: Administrative manager of the Enzymatic and Cell Engineering unit, UMR 7025 CNRS
In charge of the administrative management of the UMR, which is under three trusteeship (CNRS / UTC / UPJV), namely: Finances Human resources Communication

Since 2012: CNRS/UTC safety assistant of the UMR7025 and, in particular, in charge of the prevention of risks (biological, chemical, etc.) and the training of students and new entrants in terms of risks and safety procedures.

Main publications

Articles

Hodrogue A., Trecherel E., Cornu M., Darwiche W., Mansour A., Ait-mohand K., Verissimo T., Gomila C., Schembri C., Da Nascimento S., Elboutachfait R., Boulier A., Lorne E., Courtois J., Petit E., Toumieux S., Kovensky J., Sonnet P., Massy Z. A., Kamel S. Rossi C., Ausseil J. Oligogalacturonic acid inhibits vascular calcification by two mechanisms: inhibition of VSMC osteogenic conversion and interaction with collagen. Arteriosclerosis, Thrombosis, and Vascular Biology, 2017, 37,1391-1401.
 

Cutivet A., Schembri C., Kovensky J. & Haupt K. Molecularly imprinted microgels as enzyme inhibitors.. J. Am. Chem. Soc., 2009, 131,14699-14702.
 

Franck MERLIER

Manager of Mass Spectrometry Platform

           Contact Franck Merlier

 

 

Research

  • Mass spectrometry
  • High-resolution mass spectrometry (LC-QTOF, Agilent 6538, GC-Qexactive Orbitrap) and low-resolution mass spectrometry in tandem (Thermo Quantum GC and Agilent 6460) coupled with chromatography (LC, GC, CE)
  • Chromatography
  • HPLC, uHPLC (Hilic, RP chromatography, SEC) with fluorescence, DEDL, UV, RI detection
  • Gas chromatography (HRMS, BRMS, Ms/MS, ECD and FID)
  • Capillary electrophoresis (UV and HRMS detection)
  • GPC/SEC (MALLS)

 

Skills

Lipidomics, small molecules, primary and secondary metabolites (amino acids, sugars, acyl-Coa, polyphenols, essential oils, …)
Roles and responsabilities

Manager of the mass spectrometry platform of the Enzymatic and Cell Engineering Laboratory.
General information

Franck MERLIER studied analytical chemistry at the University of Rennes 1, Jules Vernes University of Amiens and Marie Curie High School in Nogent/Oise. He holds a master’s degree in chemistry, specializing in “Spectroscopic methods of analysis”. He is in charge of the mass spectrometry platform of the Enzymatic and Cell Engineering Laboratory at Compiègne University of Technology.
 

Articles

Xu J., Merlier F., Avalle B., Veillard V., Debré P., Haupt K., Tse Sum Bui B. Molecularly Imprinted Polymer Nanoparticles as Potential Synthetic Antibodies for Immunoprotection against HIV . ACS Appl. Mater. Interfaces, 2019, 11,9824-9831.
     ABSTRACT: We describe the preparation and characterization of synthetic antibodies based on molecularly imprinted polymer nanoparticles (MIP-NPs) for the recognition and binding of the highly conserved and specific peptide motif SWSNKS (3S), an epitope of the envelope glycoprotein 41 (gp41) of human immunodeficiency virus type 1 (HIV-1). This motif is implicated in the decline of CD4+ T cells and leads to the deterioration of the immune system during HIV infection. Therefore, the development of MIP-NPs that can target and block the 3S peptide to prevent subsequent cascade interactions directed toward the killing of CD4+ T cells is of prime importance. Because most antibodies recognize their protein antigen via a conformational or structured epitope (as opposed to a linear epitope commonly used for molecular imprinting), we employed protein molecular modeling to design our template epitope so that it mimics the three-dimensional structure fold of 3S in gp41. The resulting template peptide corresponds to a cyclic structure composed of CGSWSNKSC, with the 3S motif well orientated for imprinting. MIP-NPs with a size of 65 nm were obtained by solid-phase synthesis and were water-soluble. They were prepared by a judicious combination of multiple functional monomers affording hydrogen bonding, ionic, π–π, and hydrophobic interactions, conferring high affinity and selectivity toward both the cyclic peptide and the whole gp41 protein. These results suggest that our MIPs could potentially be used for blocking the function of the 3S motif on the virus.

Merlier F., Octave S., Tse Sum Bui B., Thomasset B. Evaluation of Performance and Validity Limits of Gas Chromatography electron ionization – Orbitrap Detector for fatty acid methyl esters analyses . Rapid Commun Mass Spectrom, 2019, in press,.
 

Medina Rangel P., Moroni E., Merlier F., Gheber L., Vago R., Tse Sum Bui B., Haupt K. Chemical Antibody Mimics Inhibit Cadherin-Mediated Cell-Cell Adhesion: A Promising Strategy for Cancer Therapy. Angewandte Chemie, 2019, In press ,.
 

Paruli E Iii., Griesser T., Merlier F., Gonzaro C., Haupt K. Molecularly imprinted polymers by thiol–yne chemistry: making imprinting even easier. Polymer Chem. , 2019, 10,4732-4739.
 

Franche A., Imbs C., Fayeulle A., Merlier F., Billamboz M. Léonard E. Zinc-mediated reactions on salicylaldehyde for Botrytis cinerea control . Chinese Chemical Letters , 2019, In press ,.
 

Ksouda G., Sellimi S., Merlier F., Facimaigne-cordin A., Thomasset B., Nasri M., Hajji M. Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive . Food Chemistry, 2019, 288,47-56.
     ABSTRACT: The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage. The GC–HRMS analysis of PSEO showed that anethole, pseudoisoeugenol and p-anisaldehyde were the main components. The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential. Additionally, PSEO displayed potent antibacterial activity against 3 Gram-positive and 3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL). The acute toxicity of PSEO was determined using mice model (LD50 = 976.2 mg/kg). The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.

Tshabuse F., Farrant J. M., Humbert L., Moura D., Rainteau D., Espinasse C., Idrissi A., Merlier F., Acket S., Rafudeen M. S., Thomasset B. & Ruelland E. Glycerolipid analysis during desication and recovery of the resurrection plant Xerophyta humilis (Bak) Dur and Schinz.. Plant Cell Environ., 2018, 41,533-547.
     ABSTRACT: Feelings in humans are mental states representing groups of physiological functions that usually have defined behavioural purposes. Feelings, being evolutionarily ancient, are thought to be coordinated in the brain stem of animals. One function of the brain is to prioritise between competing mental states and, thus, groups of physiological functions and in turn behaviour. Plants use groups of coordinated physiological activities to deal with defined environmental situations but currently have no known mental state to prioritise any order of response. Plants do have a nervous system based on action potentials transmitted along phloem conduits but which in addition, through anastomoses and other cross?links, forms a complex network. The emergent potential for this excitable network to form a mental state is unknown, but it might be used to distinguish between different and even contradictory signals to the individual plant and thus determine a priority of response. This plant nervous system stretches throughout the whole plant providing the potential for assessment in all parts and commensurate with its self?organising, phenotypically plastic behaviour. Plasticity may, in turn, depend heavily on the instructive capabilities of local bioelectric fields enabling both a degree of behavioural independence but influenced by the condition of the whole plant.

Acket S., Degournay A., Gosset M., Merlier F., Troncoso-ponce M. A. & Thomasset B. Analysis of C-13 labeling amino acids by capillary electrophoresis - High resolution mass spectrometry in developing flaxseed.. Anal. Biochem., 2018, 547,14-18.
     ABSTRACT: n context of fluxomic studies, 13C labeling analysis of amino acids are very important for solving the carbon flow calculation, because they are synthesized in various biosynthesis pathways and cellular compartments in plant cells. Traditionally, 13C labeling analysis are performed using low resolution mass spectrometry detector by GC-MS. We compared a method using capillary electrophoresis-high resolution mass spectrometry without derivatization and with better accuracy assessment of labeling measurements comparing to classical GC-MS. Our method allowed us to show that valine, leucine, alanine are not synthesized from the same pyruvate pool during the period of reserves accumulation in flax seeds.

Ksouda G., Hajji M. Sellimi S., Merlier F., Falcimaigne Cordin A., Nasri M., Thomasset B. A Systematic Comparison of 25 Tunisian Plant Species Based on Oil and Phenolic Contents, Fatty Acid Composition and Antioxidant Activity. Ind. Crops Prod, 2018, 274,432-440.
     ABSTRACT: This study investigated 25 Tunisian plant species of 13 families based on their oil and total phenolic contents. The fatty acid profiles and antioxidant activity of phenolic extracts of these plant seeds were studied in order to identify species containing unusual fatty acids and potential antioxidants. The oil content varied from 4.2 to 66.5% of DW (Dry Weight). Fatty acid profiles were determined using gas chromatography analysis coupled to flame ionization detection and high resolution mass spectrometry (GC-FID and GC-HRMS). The results showed that plant seeds were mostly composed of classic fatty acids (palmitic, oleic, linoleic and linolenic acids), while unusual fatty acids such as long-chain and odd-chain fatty acids were minor components. However, the petroselinic acid was the major component of Apiaceae family seed oil, particularly in Pimpinella saxifraga and Pimpinella major. The phenolic content of seed methanolic extracts, measured by Folin

Merlier F., Imatoukene N., Octave S., Nicaud J. M., Thomasset B. A Gas Chromatography Full Scan High Resolution Orbitrap Mass Spectrometry Method for Separation and Characterization of 3-Hydroxymethyl Pyridine Ester of Fatty Acids at Low Levels. J. Chromatogr, 2018, 123,768-778.
     ABSTRACT: Fatty acid methyl esters (FAMEs), which are commonly used to characterize lipids, have several limitations to conclude on many structures. 3-Pyridylcarbinol esters (3-PCE) are used to characterize fatty acid structures [1], in particular, to identify ring and double bond positions on the carbon chain. Chromatographic separation of these esters is complex due to their polarity and high boiling points. In this study, we used a column with high resolutive power based on ionic liquids to increase the separation quality in gas chromatography (GC). In addition, we used a high-resolution detector (Orbitrap) to limit non-specific signals and improve the detection limits. This detector could be used with a mass filter at 5?ppm for the rapid determination of 3-PCE from its characteristic ions (m/z?=?108.0441 and 92.0495). This filter allowed the identification of derivative fatty acids with good sensibility. Thus, it was possible to characterize 3-PCE by measuring the exact fragment masses to confirm structures such as C19:2n12cyclo?9.

Mourao C. A., Bokeloh F., Xu J., Prost E., Duma L., Merlier F., Bueno S. M. A., Haupt K. & Tse Sum Bui, B. Dual oriented solid phase molecular imprinting: Toward selective artificial receptors for recognition of nucleotides in water. Macromolecules, 2017, 50,7484-7490.
     ABSTRACT: We describe the synthesis of water-soluble molecularly imprinted polymer nanoparticles (MIP-NPs) as a new artificial host receptor for the recognition of adenosine monophosphate (AMP), used herein, as a model nucleotide. MIP-NPs were prepared by solid-phase synthesis on glass beads (GB) using, for the first time, immobilized Fe(III)-chelate as an affinity ligand to orientate the AMP via its phosphate group. A polymerizable thymine monomer which can induce complementary base-pairing with the adenine moiety of the nucleotide was synthesized and incorporated in the polymerization mixture to constrain the AMP in a dual-orientated configuration. The MIP-NPs were remarkably selective toward AMP as they did not bind other nucleotides, GMP, UMP, and CMP. This strategy of using the phosphate group of AMP as a hinge enables unhindered pairing of the nucleobase with its corresponding complementary base monomer and can be extended to the preparation of specific MIP receptors for other key nucleotides in aqueous conditions.

Merlier F., Jellali R., Leclerc E. Online monitoring of hepatic rat metabolism by coupling a liver biochip and a mass spectrometer. Analyst, 2017, 142,3747-3757.
     ABSTRACT: A microfluidic liver biochip was coupled with a mass spectrometer to detect in real time the drug metabolism of hepatocytes. The hepatocytes were cultivated in the biochip for 35 h. The biochip was placed in a small-scale incubator in which the temperature and CO2 concentration were controlled. The biochip was connected serially to a mass spectrometer, a peristaltic pump and a culture medium reservoir. The injection in the mass spectrometer was performed every 10 min for 11 h. The metabolism of midazolam, phenacetin, omeprazole, dextromethorphan, repaglinide, rosuvastatin, tolbutamide and caffeine was investigated. We monitored the apparition of omeprazole sulfone, hydroxy omeprazole, repaglinide glucuronide, rosuvastatin lactone, dextrorphan, 1-hydroxy midazolam, 4-hydroxy midazolam, 1,4-hydroxy midazolam, paracetamol and 1,3-methylxanthine. Although these were observed, hydroxytolbutamide, 3-methoxymorphinan and midazolam glucuronide, hydroxy repaglinide were not detected. Based on a pharmacokinetic model, we calculated in vitro intrinsic clearances in which adsorption onto the perfusion circuit was taken into account. Then, using a liver organ model, we extrapolated the in vitro intrinsic clearances to the in vivo clearances. The estimated in vivo clearances were in agreement with the literature data on rats for midazolam, dextromethorphan, phenacetin, tolbutamide and caffeine. Rosuvastatin, omeprazole and repaglinide prediction underestimated the in vivo data.

Acket S., Degournay A., Merlier F. & Thomasset B. Data documenting the comparison between the theoretically expected values of free sugars mass isotopomer composition with standards using GC-MS and LC-HRMS for Metabolic Flux Analysis.. Data in Brief, 2017, 12,108-112.
     ABSTRACT: The data presented in this article are related to the research article entitled

Aimee Rasolohery C., Ermenegilde Ralaiba B., Ayerdi Gotor A., Merlier F., Benja R., Rakotovao M., Rhazi L. Chemical Characterization and Antioxidant Potential of Athroisma protei- formis Essential Oil. The Natural Products Journal, 2017, 7,208-215.
 

Acket S., Degournay A., Merlier F. & Thomasset B. 13C labeling analysis of sugars by hight resolution-mass spectrometry for metabolic flux analysis.. Anal. Biochem., 2017, 527,45-48.
     ABSTRACT: Metabolic flux analysis is particularly complex in plant cells because of highly compartmented metabolism. Analysis of free sugars is interesting because it provides data to define fluxes around hexose, pentose, and triose phosphate pools in different compartment. In this work, we present a method to analyze the isotopomer distribution of free sugars labeled with carbon 13 using a liquid chromatography

Nestora S., Merlier F., Prost E., Haupt K., Rossi C. & Tse Sum Bui B. Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer.. J. Chromatogr. A, 2016, 1465,47-54.
 

Nestora S., Merlier F., Beyazit S., Prost E., Duma L., Baril B., Graves A., Haupt K. & Tse Sum Bui B. Plastic antibodies for cosmetics: Molecularly imprinted polymers scavenge precursors of malodors. . Angew. Chem. Int. Ed., 2016, 55,6252-6256.
 

Jellali R., Bricks T., Jacques S., Fleury M. J., Paullier P., Merlier F. & Leclerc E. Long term human primary hepatocyte cultures in a microfluidic liver biochip show maintenance of mRNA levels and higher drugs metabolisms when compared to Petri cultures.. Biopharm. Drug Disp., 2016, 37,264-275.
 

Nowacki L., Vigneron P., Rotellini L., Cazzola H., Merlier F., Prost E., Ralanairina R., Gadonna J. P., Rossi C. & Vayssade M. Betanin-enriched red beetroot (Beta vulgaris L.) extract induces apoptosis and autophagic cell death in MCF-7 cells.. Phytother. Res., 2015, 29,1964-1973.
 

Bricks T., Hamon J., Fleury M. J., Jellali R., Merlier F., Herpe Y. E., Seyer A., Regimbeau J. M., Bois F. & Leclerc E. Investigation of omeprazole and phenacetin frist-pass metabolism in humans using a microscale bioreactor and pharmacokinetic models.. Biopharm. Drug Disp., 2015, 36,275-293.
 

Bricks T., Paullier P., Legendre A., Fleury M. J., Zeller P., Merlier F., Anton P. M. & Leclerc E. Development of a new microfluidic platform integrating co-cultures of intestinal and liver cell lines.. Toxicol. In Vitro, 2014, 28,885-895.
 

Prot J. M., Maciel L., Bricks T., Merlier F., Cotton J., Paullier P., Bois F. & Leclerc E. First pass intestinal and liver metabolism of paracetamol in a microfluidic platform coupled with a mathematical modeling as a means of evaluating ADME processes in humans.. Biotechnol. Bioeng., 2014, 111,2027-2040.
 

Tanvir S., Merlier F. & Pulvin S. Biosensing of reactive intermediates produced by the photocatalytic activities of titanium dioxide nanoparticles.. J. Photochem. Photobiol. B, 2012, 110,22-27.
 

Rippa S., Zhao Y., Merlier F., Charrier A. & Perrin Y. The carnitine biosynthetic pathway in Arabidopsis thaliana shares similar features with the pathway of mamals and fungi.. Plant Physiol. Biochem., 2012, 60,109-114.
 

Prot J. M., Briffaut A. S., Letourneur F., Chafey P., Merlier F., Grandvalet Y., Legallais C. & Leclerc E. Integrated proteomic and transcriptomic investigation of the acetaminophen toxicity in liver microfluidic biochip.. PloS One, 2011, 6,e21268.
 

Tse Sum Bui B., Merlier F. & Haupt K. Towards the use of a molecularly imprinted polymer in doping analysis :selective preconcentration and analysis of testosterone and epitestosterone in human urine.. Anal. Chem., 2010, 82,4420-4427.
 

Albrecht ROSCHER

MC UPJV

            Contact Albrecht Roscher

 

 

 

Articles

Ramsay A., Fliniaux O., Quero A., Molinie R., Demailly H., Hano C., Paetz C., Roscher A., Grand E., Kovensky J., Schneider B. & Mesnard F. Kinetics of the incorporation of the main phenolic compounds into the lignan macromolecule during flaxseed development.. Food Chem., 2017, 217,1-8.
 

Ramsay A., Fliniaux O., Fang J., Molinie R., Roscher A., Grand E., Guillot X., Kovensky J., Fliniaux M. A., Schneider B. & Mesnard F. Development of an NMR metabolomics-based tool for selection of flaxseed varieties.. Metabolomics, 2014, 10,1258-1267.
 

Koubaa M., Thomasset B. & Roscher A. Quantifying 13C-label in free sugars and starch by GC-MS.. Methods Molec. Biol., 2014, 1090,121-130.
 

Roscher A., Troufflard S. & Taghki A. I. In vivo NMR for (13)C metabolic flux analysis.. Methods Molec. Biol., 2014, 1090,143-152.
 

Fliniaux O., Corbin C., Ramsay A., Renouard S., Beejmohun V., Doussot J., Falguieres A., Ferroud C., Lamblin F., Laine E., Roscher A., Grand E., Mesnard F. & Hano C. Microwave-assisted extraction of herbacetin diglucoside from flax (Linum usitatissimum L.) seed cakes and its quantification using RP-HPLC-UV system.. Molecules, 2014, 19,3025-3037.
 

Fang J., Ramsay A., Paetz C., Tatsis E. C., Renouard S., Hano C., Grand E., Fliniaux O., Roscher A., Mesnard F. & Schneider B. Concentration kinetics of secoisolariciresinol diglucoside and its biosynthetic precursor coniferin in developing flaxseed.. Phytochem. Anal., 2013, 24,41-46
  

Koubaa M., Mghaieth S., Thomasset B. & Roscher A. Gas chromatography-mass spectrometry analysis of 13C labeling in sugars for metabolic flux analysis.. Anal. Biochem., 2012, 425,183-188.
 

Labboun S., Terce-laforgue T., Roscher A., Bedu M., Restivo F. M., Velanis C. N., Skopelitis D. S., Moshou P. N., Roubelakis K. A., Suzuki A. & Hirel B. Resolving the role of plant glutamate dehydrogenase: I. In vivo real time nuclear magnetic resonance spectroscopy experiments.. Plant Cell Physiol., 2009, 50,17761-1773.
 

Bartholomeusz T. A., Molinie R., Mesnard F., Robins R. J. & Roscher A. Optimisation of 1D and 2D in vitro 1H NMR to study tropane alkaloid metabolism in Pseudomonas.. C.R. Chimie, 2008, 11,457-464.
 

Troufflard S., Roscher A., Thomasset B., Barbotin J. N., Rawsthorne S. & Portais J. C. In vivo (13)C NMR determines metabolic fluxes and steady state in linseed embryos.. Phytochem., 2007, 68,, 2341-2350.
 

Robins R. J., Molinie R., Kwiecien R. A., Paneth P., Lebreton J., Bartholomeusz T. A., Roscher A., Drager B. & Mesnard F. Progress in understanding the N-demethylation of alkaloids by exploiting isotopic techniques. . Phytochem. Rev., 2007, 6,51-63.
 

Bartholomeusz T. A., Mesnard F., Felpin F. X., Lebreton J., Robins R. J. & Roscher A. Optimised NMR detection of 13C-2H double labelling in small molecules. . C.R. Chimie, 2006, 9,514-519.
 

Houyou N., Pau-roblot C. & Roscher A. 15N relaxation and quantification of 15N labelled metabolites in cell extracts. . C.R. Chimie, 2006, 9,320-524.
 

Isabelle GOSSELIN

MC UPJV

      Contact Isabelle Gosselin

 

Articles

Huet G., Hadad C., Husson E., Laclef S., Lambertyn V., Araya Farias M., Jamali A., Courty M., Alayoubi R., Gosselin I., Sarazin C. And Van Nhien A. Straightforward extraction and selective bioconversion of high purity chitin from Bombyx eri larva: Toward an integrated insect biorefinery . Carbohydr. Polym. , 2020, 228,115382 .
 

Huet G.,  araya Farias M.,  alayaoubi R., Laclef S., Bouvier, B.  gosselin I.,  cézard C., Roulard R., Courty M., Hadad C.,  husson E.,  sarazin C., Nguyen Van Nhien a. New Biobased-Zwitterionic Ionic Liquids: Efficiency and Biocompatibility for the Development of Sustainable Biorefinery Processes. . Green Chem. , 2020, 22 ,2935 .
 

Rondeau M., Esmaeel Q., Crouzet J., Blin Pauline., Gosselin I., Sarazin C., Pernes M., Beugrand J., Wisniewski-dyé F., Vial L., Faure D. Clément C., Ait Barka E., Jacquard C. Sanchez L. Biofilm constructing variants of Paraburkholderia phytofirmans PsJN outcompete the wild-type form in free-living and static conditions but not in planta . Applied and Environmental Microbilogu , 2019, 85 ,e02670-18 .
 

Araya-farias M., Husson E., Saavedra-torrico J., Gérard D., Roulard R., Gosselin I., Rakotoarivonina H., Lambertyn V. Rémond C. Sarazin C. Wheat Bran Pretreatment by Room Temperature Ionic Liquid-Water Mixture: Optimization of Process Conditions by PLS-Surface Response Design . Fontiers in Chemistry , 2019, 7 ,585.
 

Alayoubi R., Mehmood N., Husson E., Kouzayha A., Tabcheh M., Chaveriat L, Sarazin C., Gosselin I. Low temperature ionic liquid pretreatment of lignocellulosic biomass to enhance bioethanol yield . Renewable Energy , 2019, 145 ,1808-1816 .
 

Mehmood N., Alayoubi R., Husson E., Jacquard C., Buchs J., Sarazin C. & Gosselin I. Kluyveromyces marxianus, an attractive yeast for ethanolic fermentation in the presence of imidazolium ionic liquids.. Int. J. Molec. Sci., 2018, 19,887.
     ABSTRACT: Imidazolium ionic liquids (ILs) are promising solvents for lignocellulosic biomass (LCB) pretreatment and allow the achievement of higher ethanolic yields after enzymatic hydrolysis and ethanolic fermentation. However, residual ILs entrapped in pretreated biomass are often toxic for fermentative microorganisms, but interaction mechanisms between ILs and cells are still unknown. Here we studied the effects of 1-ethyl-3-methylimidazolium acetate [Emim][OAc] and 1-ethyl-3-methylimidazolium methylphosphonate [Emim][MeO(H)PO2] on Kluyveromyces marxianus, a thermotolerant ethanologenic yeast. Morphological impacts induced by ILs on K. marxianus were characterized by Scanning Electron Microscopy analysis and showed wrinkled, softened, and holed shapes. In Yeast-Malt-Dextrose (YMD) medium, K. marxianus tolerated IL additions up to 2% for [Emim][OAc] and 6% for [Emim][MeO(H)PO2]. Below these thresholds, some IL concentrations enhanced ethanolic yields up to +34% by switching the metabolic status from respiratory to fermentative. Finally, K. marxianus fermentation was applied on several substrates pretreated with [Emim][OAc] or [Emim][MeO(H)PO2] and enzymatically hydrolyzed: a model long fiber cellulose and two industrial LCBs, softwood (spruce) and hardwood (oak) sawdusts. The maximum ethanolic yields obtained were 1.8 to 3.9 times higher when substrates were pretreated with imidazolium ILs. Therefore K. marxianus is an interesting fermentative yeast in a second-generation bioethanol process implying IL pretreatment

Mehmood N., Husson E., Jacquard C., Wewetzer S., Buchs J., Sarazin C. & Gosselin I. Impact of two ionic liquids, 1-ethyl-3-methylimidazolium acetate and 1-ethyl-3-methylimidazolium methylphosphonate, on Saccharomyces cerevisiae: metabolic, physiologic, and morphological investigations.. Biotechnol. Biofuels , 2015, 8,n.17.
 

Chaveriat L., Gosselin I., Machut C. & Martin P. Synthesis, surface tension properties and antibacterial activities of amphiphilic D-galactopyranose derivatives.. Eur. J. Med. Chem., 2013, 62,177-186.
 

Husson E., Buchoux S., Avondo C., Cailleu D., Djellab K., Gosselin I., Wattraint O. & Sarazin C. Enzymatic hydrolysis of ionic liquid-pretreated celluloses: contribution of CP-MAS, 13C NMR and SEM.. Bioresource Technol., 2011, 102,7335-7342.
 

Brique A., Devassine J., Pilard S., Cailleu D. & Gosselin I. Osmoregulated trehalose-derived oligosaccharides in Sinorhizobium meliloti.. FEBS Letters, 2010, 584,3661-3666.
 

Eric HUSSON

MC UPJV

       Contact Eric Husson

 

Research

Member of the theme “Plant Metabolism and Bioresources”.

Room temperature ionic liquids and enzymes for the development of biorefinery strategies.
Skills

Enzymology in conventionnal and non-conventionnal media ; Ionic Liquids pretreatment of lignocellulosic, or chitinous biomasses; Separation / quantification techniques and structural characterization.
Roles and responsabilities

Involved in several reasearch projects (partner or leader), co-supervizer of PhD students.

Teaching in biochemistry, enzymology and microbiology at bachelor and master levels.
General information

  • Since 2011- Assistant Professor in Biochemistry – Enzyme and Cell Engineering UMR CNRS 7025, Université de Picardie Jules Verne, Amiens, France
  • 2011 – Post-doctoral position, Department of Food and Nutrition Sciences, Laval University, Québec, QC, Canada
  • 2010 – Post-doctoral position, Enzyme and Cell Engineering UMR CNRS 7025, Université de Picardie Jules Verne, Amiens, France
  • 2009 – Post-doctoral position (ATER), Laboratory of Reactions and Process Engineering UMR CNRS 7274, Université de Lorraine, Nancy, France
  • 2005-2008 – PhD, Laboratory of Reactions and Process Engineering UMR CNRS 7274, Université de Lorraine, Nancy, France

 

Personal interests

Scientific communication in priority education areas

 

Articles

Hadad C., Husson E., Van Nhien A. N. Conversion of Chitin in Ionic Liquids. In: Zhang S. . Springer, Singapore , 2020, ,
  

Huet G.,  araya Farias M.,  alayaoubi R., Laclef S., Bouvier, B.  gosselin I.,  cézard C., Roulard R., Courty M., Hadad C.,  husson E.,  sarazin C., Nguyen Van Nhien a. New Biobased-Zwitterionic Ionic Liquids: Efficiency and Biocompatibility for the Development of Sustainable Biorefinery Processes. . Green Chem. , 2020, 22 ,2935 .
 

Huet G., Hadad C., Husson E., Laclef S., Lambertyn V., Araya Farias M., Jamali A., Courty M., Alayoubi R., Gosselin I., Sarazin C. And Van Nhien A. Straightforward extraction and selective bioconversion of high purity chitin from Bombyx eri larva: Toward an integrated insect biorefinery . Carbohydr. Polym. , 2020, 228,115382 .
 

Husson E., Hulin L., Hadad C., Boughanmi C., Stevanovic T., Sarazin C. Acidic Ionic Liquid as Both Solvent and Catalyst for Fast Chemical Esterification of Industrial Lignins: Performances and Regioselectivity . Fontiers in Chemistry , 2019, 7 ,578.
     ABSTRACT: 

Araya-farias M., Husson E., Saavedra-torrico J., Gérard D., Roulard R., Gosselin I., Rakotoarivonina H., Lambertyn V. Rémond C. Sarazin C. Wheat Bran Pretreatment by Room Temperature Ionic Liquid-Water Mixture: Optimization of Process Conditions by PLS-Surface Response Design . Fontiers in Chemistry , 2019, 7 ,585.
 

Alayoubi R., Mehmood N., Husson E., Kouzayha A., Tabcheh M., Chaveriat L, Sarazin C., Gosselin I. Low temperature ionic liquid pretreatment of lignocellulosic biomass to enhance bioethanol yield . Renewable Energy , 2019, 145 ,1808-1816 .
 

Husson E., Auxenfans T., Herbaut M., Baralle M., Lambertyn V., Rakotoarivonina H., Remond C. & Sarazin C. Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases.. Bioresource Technol., 2018, 251,280-287.
     ABSTRACT: Sequential and simultaneous strategies for fractioning wheat straw were developed in combining 1-ethyl-3-methyl imidazolium acetate [C2mim][OAc], endo-xylanases from Thermobacillus xylanilyticus and commercial cellulases. After [C2mim][OAc]-pretreatment, hydrolysis catalyzed by endo-xylanases of wheat straw led to efficient xylose production with very competitive yield (97.6?

Mehmood N., Alayoubi R., Husson E., Jacquard C., Buchs J., Sarazin C. & Gosselin I. Kluyveromyces marxianus, an attractive yeast for ethanolic fermentation in the presence of imidazolium ionic liquids.. Int. J. Molec. Sci., 2018, 19,887.
     ABSTRACT: Imidazolium ionic liquids (ILs) are promising solvents for lignocellulosic biomass (LCB) pretreatment and allow the achievement of higher ethanolic yields after enzymatic hydrolysis and ethanolic fermentation. However, residual ILs entrapped in pretreated biomass are often toxic for fermentative microorganisms, but interaction mechanisms between ILs and cells are still unknown. Here we studied the effects of 1-ethyl-3-methylimidazolium acetate [Emim][OAc] and 1-ethyl-3-methylimidazolium methylphosphonate [Emim][MeO(H)PO2] on Kluyveromyces marxianus, a thermotolerant ethanologenic yeast. Morphological impacts induced by ILs on K. marxianus were characterized by Scanning Electron Microscopy analysis and showed wrinkled, softened, and holed shapes. In Yeast-Malt-Dextrose (YMD) medium, K. marxianus tolerated IL additions up to 2% for [Emim][OAc] and 6% for [Emim][MeO(H)PO2]. Below these thresholds, some IL concentrations enhanced ethanolic yields up to +34% by switching the metabolic status from respiratory to fermentative. Finally, K. marxianus fermentation was applied on several substrates pretreated with [Emim][OAc] or [Emim][MeO(H)PO2] and enzymatically hydrolyzed: a model long fiber cellulose and two industrial LCBs, softwood (spruce) and hardwood (oak) sawdusts. The maximum ethanolic yields obtained were 1.8 to 3.9 times higher when substrates were pretreated with imidazolium ILs. Therefore K. marxianus is an interesting fermentative yeast in a second-generation bioethanol process implying IL pretreatment

Brahim M., Checa Fernandez B. L., Regnier O., Bousseta N., Grimi N., Sarazin C., Husson E., Vorobiev E., Brosse N. Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straw’s lignin and pulps. Bioresource Technology, 2017, 237,42308
  

Husson E., Hadad C., Huet G., Laclef S., Lesur D., Lambertyn V., Jamali A., Gottis S., Sarazin C., Nguyen Van Nhien A. The effect of room temperature ionic liquids on the selective biocatalytic hydrolysis of chitin via sequential or simultaneous strategies. Greeen Chemistry, 2017, 19,4122-4131.
     ABSTRACT: An efficient conversion of chitin, the second most abundant renewable polymer on the Earth, into N-acetylglucosamine and N,N?-diacetylchitobiose, using room temperature ionic liquids (RTILs) and commercially available chitinases is described for the first time. The sequential strategy consists of the use of RTILs to pretreat chitin under mild conditions as a first step before enzymatic hydrolysis. [C2mim][OAc] (1-ethyl-3-methyl imidazolium) pretreatment provides an efficient production of N-acetylglucosamine (185.0

Brahim M., Ferandez B. L. C., Regnier O., Boussetta N., Grimi N., Sarazin C., Husson E., Vorobiev E., Brosse N Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straw's lignin and pulps. Bioressource Technology , 2017, 237,11-19.
     ABSTRACT: In this study, ultrasound (US) and high voltage electrical discharges (HVED) were combined with chemical treatments (soda or organosolv) for rapeseed straw delignification. Delignification was improved by both physical pretreatments. US increased the extractability of hemicelluloses and HVED induced a partial degradation of cellulose. Best synergies were observed for HVED-soda and US-organosolv treatments. The obtained lignin fractions were characterized with 13C NMR and 2D 1H

Auxenfans T., Husson E. & Sarazin C. Simultaneous pretreatment and enzymatic saccharification of (ligno)celluloses in aqueous-ionic liquid media: a compromise.. Biochem. Eng. J., 2017, 117,77-86.
     ABSTRACT: In view of decreasing the amount of IL to achieve efficient simultaneous pretreatment and saccharification, a comprehensive study was undertaken. Different types of lignocellulosic biomasses were investigated in various enzymatic aqueous-IL systems including 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) or 1-ethyl-3-methylimidazolium methylphosphonate ([C2mim][MeO(H)PO2]). To better understand how 10% (v/v) of IL in the reaction medium led to the highest yields of glucose without fractionation from sawdust, distinct cellulosic models were then used as substrates. Kinetic studies demonstrated that both ILs affect synergistic action of cellulolytic enzymes depending on both constitutive anion and cellulosic substrate. Concentrations above 10% v/v of ILs deactivated cellulase even on highly digestible model substrates. ? and ? Kamlet-Taft parameters constituted more physicochemical pertinent indicators than apparent pH value to investigate effects of IL on cellulase performances. Fine description of these effects was proposed onto individual EG, CBH and BG. [C2mim][MeO(H)PO2] was demonstrated a better compatible IL for enzymes up to a concentration of 30% (v/v). The efficiency of simultaneous pretreatment and saccharification was governed by a compromise between better substrate accessibility and enzyme deactivation.

Mehmood N., Husson E., Jacquard C., Wewetzer S., Buchs J., Sarazin C. & Gosselin I. Impact of two ionic liquids, 1-ethyl-3-methylimidazolium acetate and 1-ethyl-3-methylimidazolium methylphosphonate, on Saccharomyces cerevisiae: metabolic, physiologic, and morphological investigations.. Biotechnol. Biofuels , 2015, 8,n.17.
 

Hulin L., Husson E., Bonnet J. P., Stevanovic T. & Sarazin C. Enzymatic transesterification of kraft lignin with long acyl chains in ionic liquids.. Molecules, 2015, 20,16334-16353.
 

Auxenfans T., Buchoux S., Husson E. & Sarazin C. Efficient enzymatic saccharification of Miscanthus: energy-saving by combinig dilute acid and ionic liquid pretreatments.. Biomass Bioenerg., 2014, 62,82-92.
 

Auxenfans T., Buchoux S., Larcher T., Husson G., Husson E. & Sarazin C. Enzymatic saccharification and structural properties of industrial wood sawdust: recycled ionic liquids pretreatments.. Energ. Convers. Manage., 2014, 88,1094-1103.
 

Doyen A., Husson E. & Bazinet L. Use of an electrodialytic reactor for the simultaneous ß-lactoglobulin enzymatic hydrolysis and fractionation of generated bioactive peptides.. Food Chem., 2013, 136,1193-1202.
 

Husson E., Araya-farias M., Dejardins Y. & Bazinet L. Selective anthocyanins enrichment of cranberry juice by electrophoresis with multiple ultrafiltration membranes stacked.. Innov. Food Sci. Emerg. Technol. , 2013, 17,153-162.
 

Husson E., Araya-farias M., Gagne A. & Bazinet L. Selective anthocyanins enrichment of cranberry juice by electrodialysis with filtration membranes: influence of membrane characteristics.. J. Membrane Sci., 2013, 448,114-124.
 

Auxenfans T., Buchoux S., Djellab K., Avondo C., Husson E. & Sarazin C. Mild pretreatment and enzymatic saccharification of cellulose with recycled ionic liquid towards one-batch process.. Carbohydr. Polym., 2012, 90,805-813.
 

Husson E., Buchoux S., Avondo C., Cailleu D., Djellab K., Gosselin I., Wattraint O. & Sarazin C. Enzymatic hydrolysis of ionic liquid-pretreated celluloses: contribution of CP-MAS, 13C NMR and SEM.. Bioresource Technol., 2011, 102,7335-7342.
 

BOOK

Chavelot I, Arab-tehrany E, Husson E, Gerardun C, Vandamme E, Luis Revuelta J. Application of carnosine and its functionalised derivatives. Industrial Biotechnology of vitamins, biopigments, and antioxidants , 2016, E.J. Vandamme & J.L. Revuelta eds. ,pp. 421-444
  

Patent

E. Husson, C. Sarazin, H. Ducatel, I. Murrieta-pazos, G. Ravot Procédé de traitement de la biomasse. , 2017, ,.
 

Irene MAFFUCCI

MC UTC

        Contact Irene Maffucci

Research

Within the theme “Biomimicry and Biomolecular Diversity”, she is involved in the rational design of potentially bioactive molecules, such as molecular imprinted polymers (MIPs) targeting macrobiomolecules, aptamers and peptidomimetics. This implies the application of multiple molecular modeling techniques, from docking, to enhanced sampling molecular dynamics, to binding affinity predictions.

In addition, she is also interested in the design and optimisation of protein-protein interaction modulators within virtual screening procedures.

 

Skills

During her research career, she developed different molecular modeling competences, such as docking, peptide secondary structure prediction, binding affinity prediction, enzymatic activity investigation and protein thermostability studies. This implied the mastering of docking, molecular dynamics, ehnanced molecular dynamics and mixed QM/MM techniques.

 

Roles and responsabilities

She is responsible of setting up the bioinformatic core within the GEC. She also takes part in the teaching activities at UTC, being in charge of the Unité d’Enseignement focused on the methods and tools for bioinformatics.

 

General information

After a master in Chemistry and Pharmaceutical Technologies in 2012 at the University of Milan, in 2015 she got a PhD in Drug Chemistry at the University of Milan under the supervision of Prof. Alessandro Contini and working on the development and application of computational methods for the design of protein-protein interactions modulations. Within her PhD, in 2014, she collaborated with the organic chemists of the Prof. Clayden’s group at the University of Manchester on the rational conformational control of helical peptides. From 2016 to 2018, under the supervision of Dr. Damien Laage, Fabio Sterpone and Guillaume Stirnemann, she held a post-doctoral position within a collaboration between the theoretical pole of the Ecole Normale Supérieure and the Laboratoire de Biochimie Théorique at the Institut de Biologie Physico-Chimique in Paris. Her project aimed to understand the effect of multiple mutations on the structural stability and activity of the dihydrofolate reductase. She joined the group GEC in September 2018.

 

Personal interests 

She likes reading, cinema and theatre. She loves travelling and cooking.

 

Articles

Chaaya N., Shahsavarian M., Maffucci I., Friboulet A., Offmann B., Leger J. B., Rousseau S., Avalle B., Padiolleau-lefevre S. Genetic background and immunological status influence B cell repertoire diversity in mice . Scientific Reports , 0, 9,14261 .
 

Adrian TRONCOSO-PONCE

MC-UTC

General information

After obtaining two bachelor degrees, in Pharmacy and Biochemistry (the latter one honored with the Extraordinary Award of Bachelor), I initiated my Ph.D. program on Plant Science. More than a decade later and with more grey hair, I am a current “Maître de conferences” and former “Chaire d’excellence” at the UTC. I have conducted research in Europe, at the Spanish Scientific Research Higher Council (CSIC) and the Institut Jean-Pierre Bourgin (INRAE) where I was granted with the Marie Skłodowska-Curie fellowship, and in the USA, at Michigan State University.

Research

Over the years, I have conducted research on plant metabolism. These investigations were carried out in model plants, agricultural staple crops, and potential new alternative oil crops. The studies explore diverse aspects of plant central and secondary metabolism, including characterizing enzymes involved in glycolysis and lipoic acid synthesis, employing metabolic engineering to tailor lipid composition and enhance carotenoid production, analyzing metabolic pathways through fluxomics, investigating the role of epigenetics in fatty acid synthesis and the interaction between the circadian clock and lipid synthesis in seeds. Other aspects of plant metabolism are also under investigation, such as the squalene biosynthetic pathway in plants that accumulate high levels of this isoprenoid and the roles of diacylglycerol-kinases in plant responses to environmental signals.

Skills

Biochemistry, molecular biology and enzymology.

Roles and responsabilitie

In addition to my research and teaching activities in the Engineering and Master’s programs, I also participate in the implementation of sustainable development at GEC and coordinate the greenhouse platform.

Personal interests

I am interested on the past and present of humans, from paleoanthropology to modern history. My interest in human evolution stemmed from my fascination with paleontology. Additionally, I hold a strong interest in a different aspect of human interactions: boxing.

Contact Adrian Troncoso-Ponce

Benjamin BOUVIER

Benjamin BOUVIER

CR CNRS
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Francisco RAMOS MARTIN

MC UPJV
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Mirian KUBO

ECC UTC
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Eric RUELLAND

CR CNRS - Co-Animator Theme PMB
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Stéphane OCTAVE

Innovation Correspondent - Member Steering Committee of "Science Festival" UTC

          Contact Stéphane Octave

 

Research

I’m working at the interface between the theme “Plant Metabolism and Bioresources” and “Biomimicry and Biomolecular Recognition”.

My research focuses on the exploration and exploitation of molecular diversity to modulate plant metabolism. The goal is to provide tools to optimize plant productivity either by acting directly on the metabolic pathways of interest, or by helping plants to adapt themselves to biotic and abiotic stresses.

 

Skills

  • Management: scientific projects and programs
  • Valorization and transfert

 

Roles and responsabilities

  • Referent for Innovation (for INSB)
  • Member of the steering comity for « Fete de la Science » in UTC

 

General information

PhD in biology from the University of Poitiers (in 2005).

Work focused on the study of the eutypiosis of the vine along two axes: understand the dialogue between the plant and the pathogenic fungus on the one hand and on the other hand develop tools to fight against infection. This latter approach was based on the use of natural molecules (amino acids and derivatives) and the use of pathogenic effectors to develop an immunoassay detection tool.

Joined UTC as research engineer in 2006 in the Research Direction to assist researchers in their projects, from preparing call’s answer to the exploitation of results. Also involved in University’s programs, in particular in structuration and implementation of the ITE PIVERT.

Member of the laboratory since July 2017.

 

Main publications

Articles

Merlier F., Octave S., Tse Sum Bui B., Thomasset B. Evaluation of Performance and Validity Limits of Gas Chromatography electron ionization – Orbitrap Detector for fatty acid methyl esters analyses . Rapid Commun Mass Spectrom, 2019, in press,.
 

Merlier F., Imatoukene N., Octave S., Nicaud J. M., Thomasset B. A Gas Chromatography Full Scan High Resolution Orbitrap Mass Spectrometry Method for Separation and Characterization of 3-Hydroxymethyl Pyridine Ester of Fatty Acids at Low Levels. J. Chromatogr, 2018, 123,768-778.
     ABSTRACT: Fatty acid methyl esters (FAMEs), which are commonly used to characterize lipids, have several limitations to conclude on many structures. 3-Pyridylcarbinol esters (3-PCE) are used to characterize fatty acid structures [1], in particular, to identify ring and double bond positions on the carbon chain. Chromatographic separation of these esters is complex due to their polarity and high boiling points. In this study, we used a column with high resolutive power based on ionic liquids to increase the separation quality in gas chromatography (GC). In addition, we used a high-resolution detector (Orbitrap) to limit non-specific signals and improve the detection limits. This detector could be used with a mass filter at 5?ppm for the rapid determination of 3-PCE from its characteristic ions (m/z?=?108.0441 and 92.0495). This filter allowed the identification of derivative fatty acids with good sensibility. Thus, it was possible to characterize 3-PCE by measuring the exact fragment masses to confirm structures such as C19:2n12cyclo?9.

Roblin G., Octave S., Faucher M., Fleurat-lessard P. & Berjeaud J. M. Cysteine: A multifaceted amino acid involved in signaling, plant resistance and antifungal development.. Plant Physiol. Biochem., 2018, 129,77-89.
     ABSTRACT: Early effects induced by cysteine were monitored using the model of Mimosa pudica pulvinar cells. Rapid dose-dependent membrane depolarization (within seconds) and modification of proton secretion (within minutes) were triggered at cysteine concentrations higher than 0.1?mM. These effects did not result from a modification of the plasma membrane H+-ATPase activity nor from a protonophore effect as shown by assays on plasma membrane vesicles isolated from pulvinar tissues. In a 0.5

Octave S., Fleurat-lessard P., Roblin G. Diagnosis of Eutypa lata infection by immunological detection in grapevine dying arm disease. Journal of plant Pathology, 2009, 91,321-300.
 

Octave S. &thomas D. Biorefinery: Toward an industrial metabolism.. Biochimie, 2009, 91,659-664.
 

Octave S., Fleurat-lessard P., Roblin G. Effects of non-glycosylated and glycosylated polypeptides secreted by the grapevine pathogen Eutypa lata on the structural features and membrane processes in grapevine cells . Journal of plant Pathology , 2008, 90,221-224.
 

Octave S., Roblin G., Vachaud M., Fleurat-lessard P. Polypeptide metabolites secreted by the fungal pathogen Eutypa lata participate in Vitis vinifera L. to cell structure damage observed in Eutypa dieback . Functional Plant Biology , 2006, 33 ,297-307 .
 

Octave S., Amborabe B. E., Fleurat-lessard P., Berges T., Roblin G. Modifications of plant cell activities by peptidic metabolites excreted by Eutypa lata, a vineyard fungal pathogen . Physiologia Plantarum , 2006, 128 ,103-115 .
 

Rudelle J., Octave S., Kaid-arche M., Roblin G., Fleurat-lessard P.  Structural modifications induced by Eutypa lata in the xylem of trunk and canes of Vitis vinifera . Functional Plant Biology , 2005, 32 ,537-547 .
 

Amborabe B. E., Octave S., Roblin G. Influence of temperature and nutritional requirements for mycelial growth of Eutypa lata, a vineyard pathogenic fungus . C.R. Biologies , 2005, 328 ,263-270 .
 

Octave S., Amborabe B. E., Luini E., Ferreira T., Fleurat-lessard P., Roblin G. Antifungal effect of cysteine towards the fungus Eutypa lata . Plant Physiology and Biochemistry , 2005, 43 ,1006-1013 .
 

Bernadette TSE SUM BUI

            Contact Bernadette Tse Sum Bui

Research

Member of the theme “Biomimicry and Biomolecular Diversity”.

Development of Molecular Imprinted Polymers (MIPs) and composite materials based on MIP for bioanalysis (separation, solid-phase extraction), biosensors, cellular bioimaging, controlled release of drugs, immunoassays (fluorescence, radioactivity, colorimetry) and enzyme inhibitors, odor and air pollutants suppresors. The developed MIPs target endocrine disruptors, antibiotics, mycotoxins, hormones, drugs, pesticides, nucleotides, amino acids, biogenic amines, cancer biomarkers (glycans, proteins, …) and they can be applied in domains such as nanomedicine, environment, agri-food, doping, cosmetology, biotechnology, …

 

General information

  • 2018: Member of the Action COST “Innovation with Glycans: new frontiers from synthesis to new biological targets” (INNOGLY)
  • 2018: Member of the association Healthcare & Biological Sciences Research Association (HBSRA)
  • 2017: CNRS crystal medal
  • 2017: Member of the organization committee of the international congress ‘Affinity 2017, 22nd Biennal Meeting of International Society for Molecular Recognition’, Paris
  • 2013: Member of the  organization committee ‘2nd NANODRUG meeting and summer school’, Biarritz
  • 2012: Prix Collegium UTC-CNRS-INSIS
  • 2012: Member of the organization committee of the 7th international congress ‘MIP 2012, Molecularly Imprinted Polymers-Science and Technology’, Paris

Degrees

  • 1991: PhD in biotechnology, UTC
  • 2001: HDR en biotechnology, Université Pierre et Marie Curie (UPMC), Paris

Profesionnal experience

  • 1993: Post-doc, Laboratoire de Chimie Organique et Biologique, UPMC
  • 1997: CNRS research engineer, UPMC
  • 2006: CNRS research engineer, UTC

Supervision

3 post-docs, 13 PhDs, 3 masters
Bin Li: Molecularly imprinted polymers for applications in cosmetology (2013).
Xuan-Anh Ton: Fiber optic chemical sensors based on molecularly imprinted polymers for the detection of mycotoxins (2013).
Selim Beyazit: Functional nanoparticles for biomedical applications (2014).
Jacqueline Maximilien: Studies of the impact of core-shell polystyrene nanoparticles on cell membranes and biomimetic models (2015).
Maria Panagiotopoulou: Organic-inorganic composite materials for specific recognition and optical detection of environmental, food and biomedical analytes (2016).
Sofia Nestora: Molecularly imprinted polymers as selective sorbents for recognition in complex aqueous samples (2017).
Jingjing Xu: Solid-phase synthesis of molecularly imprinted polymer nanoparticles for protein recognition (2017).
Paulina Rangel: Molecularly imprinted nanostructures for cell differentiation.
Alejandra Mier:  Molecularly imprinted polymer nanocomposites for cancer cell recognition and cell imaging.

Main publications

Articles

Merlier F., Octave S., Tse Sum Bui B., Thomasset B. Evaluation of Performance and Validity Limits of Gas Chromatography electron ionization – Orbitrap Detector for fatty acid methyl esters analyses . Rapid Commun Mass Spectrom, 2019, in press,.
 

Mier A., Nestora S., Medina Rangel P., Rossez Y., Haupt K., Tse Sum Bui B. Cytocompatibility of Moleculary Imprinted Polymers for Deodorants : Evaluation on Human Keratinocytes and Axillary-Hosted Bacteria . ACS Appl. Mater. Interfaces , 2019, 2 ,3439-3447    ABSTRACT: https://doi.org/10.1021/acsabm.9b00388

Xu J., Merlier F., Avalle B., Veillard V., Debré P., Haupt K., Tse Sum Bui B. Molecularly Imprinted Polymer Nanoparticles as Potential Synthetic Antibodies for Immunoprotection against HIV . ACS Appl. Mater. Interfaces, 2019, 11,9824-9831.
     ABSTRACT: We describe the preparation and characterization of synthetic antibodies based on molecularly imprinted polymer nanoparticles (MIP-NPs) for the recognition and binding of the highly conserved and specific peptide motif SWSNKS (3S), an epitope of the envelope glycoprotein 41 (gp41) of human immunodeficiency virus type 1 (HIV-1). This motif is implicated in the decline of CD4+ T cells and leads to the deterioration of the immune system during HIV infection. Therefore, the development of MIP-NPs that can target and block the 3S peptide to prevent subsequent cascade interactions directed toward the killing of CD4+ T cells is of prime importance. Because most antibodies recognize their protein antigen via a conformational or structured epitope (as opposed to a linear epitope commonly used for molecular imprinting), we employed protein molecular modeling to design our template epitope so that it mimics the three-dimensional structure fold of 3S in gp41. The resulting template peptide corresponds to a cyclic structure composed of CGSWSNKSC, with the 3S motif well orientated for imprinting. MIP-NPs with a size of 65 nm were obtained by solid-phase synthesis and were water-soluble. They were prepared by a judicious combination of multiple functional monomers affording hydrogen bonding, ionic, π–π, and hydrophobic interactions, conferring high affinity and selectivity toward both the cyclic peptide and the whole gp41 protein. These results suggest that our MIPs could potentially be used for blocking the function of the 3S motif on the virus.

Medina Rangel P., Caclef S., Xu J., Panagiotopoulou M., Kovensky J., Tse Sum Bui B., Haupt K. Solid-phase synthesis of moleculary imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans. Scientific Reports, 2019, 9,3923.
 

Medina Rangel P., Moroni E., Merlier F., Gheber L., Vago R., Tse Sum Bui B., Haupt K. Chemical Antibody Mimics Inhibit Cadherin-Mediated Cell-Cell Adhesion: A Promising Strategy for Cancer Therapy. Angewandte Chemie, 2019, In press ,.
 

Xu J., Prost E., Haupt K. & Tse Sum Bui B. Direct and sensitive determination of trypsin in human urine using a water-soluble signaling fluorescent molecularly imprinted polymer nanoprobe. . Sensor. Actuat. B-Chem. , 2018, 258,10-17.
     ABSTRACT: https://doi.org/10.1016/j.snb.2017.11.077

Mattsson L., Xu J., Preininger C., Tse Sum Bui B. & Haupt K. Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix.. Talanta, 2018, 181,190-196.
     ABSTRACT: We developed a competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines in fish samples. MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a complex sample matrix, such as fish extract, into the assay changes the environment and the binding conditions, therefore the importance of the sample preparation is extensively discussed. Several extraction and purification methods for fish were comprehensively studied, and an optimal clean-up procedure for fish samples using liquid-liquid extraction was developed. The feasibility of the competitive MIP assay was shown in the purified fish extract over a broad histamine range (1 ? 430ʵM). The MIP had the highest affinity towards histamine, but recognized also the structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine, providing simultaneous analysis and assessment of the total amount of biogenic amines.

Demir B., Lemberger M. M., Panagiotopoulou M., Medina Rangel P. X., Timur S., Hirsch T., Tse Sum Bui B., Wegener J. & Haupt K. Tracking hyaluronan: molecularly imprinted polymer coated carbon dots for cancer cell targeting and imaging.. ACS Appl. Mater. Interfaces, 2018, 10,3305-3313.
     ABSTRACT: War against cancer constantly requires new affinity tools to selectively detect, localize, and quantify biomarkers for diagnosis or prognosis. Herein, carbon nanodots (CDs), an emerging class of fluorescent nanomaterials, coupled with molecularly imprinted polymers (MIPs), are employed as a biocompatible optical imaging tool for probing cancer biomarkers. First, N-doped CDs were prepared by hydrothermal synthesis using starch as carbon source and l-tryptophan as nitrogen atom provider to achieve a high quantum yield of 25.1

Xu J, Haupt K. & Tse Sum Bui B. Core-shell molecularly imprinted polymer nanoparticles as synthetic antibodies in a sandwich fluoroimmunoassay for trypsin determination in human serum.. ACS Appl. Mater. Interfaces, 2017, 9, 24476?24483.
     ABSTRACT: We describe the application of a fluorescently labeled water-soluble core

Mourao C. A., Bokeloh F., Xu J., Prost E., Duma L., Merlier F., Bueno S. M. A., Haupt K. & Tse Sum Bui, B. Dual oriented solid phase molecular imprinting: Toward selective artificial receptors for recognition of nucleotides in water. Macromolecules, 2017, 50,7484-7490.
     ABSTRACT: We describe the synthesis of water-soluble molecularly imprinted polymer nanoparticles (MIP-NPs) as a new artificial host receptor for the recognition of adenosine monophosphate (AMP), used herein, as a model nucleotide. MIP-NPs were prepared by solid-phase synthesis on glass beads (GB) using, for the first time, immobilized Fe(III)-chelate as an affinity ligand to orientate the AMP via its phosphate group. A polymerizable thymine monomer which can induce complementary base-pairing with the adenine moiety of the nucleotide was synthesized and incorporated in the polymerization mixture to constrain the AMP in a dual-orientated configuration. The MIP-NPs were remarkably selective toward AMP as they did not bind other nucleotides, GMP, UMP, and CMP. This strategy of using the phosphate group of AMP as a hinge enables unhindered pairing of the nucleobase with its corresponding complementary base monomer and can be extended to the preparation of specific MIP receptors for other key nucleotides in aqueous conditions.

Panagiotopoulou M., Kunath S., Medina-rangel P. X., Haupt K. & Tse Sum Bui B. Fluorescent molecularly imprinted polymers as plastic antibodies for selective labeling and imaging of hyaluronan and sialic acid on fixed and living cells.. Biosens. Bioelectron., 2017, 88,85-93.
     ABSTRACT: Altered glycosylation levels or distribution of sialic acids (SA) or hyaluronan in animal cells are indicators of pathological conditions like infection or malignancy. We applied fluorescently-labeled molecularly imprinted polymer (MIP) particles for bioimaging of fixed and living human keratinocytes, to localize hyaluronan and sialylation sites. MIPs were prepared with the templates D-glucuronic acid (GlcA), a substructure of hyaluronan, and N-acetylneuraminic acid (NANA), the most common member of SA. Both MIPs were found to be highly selective towards their target monosaccharides, as no cross-reactivity was observed with other sugars like N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, D-glucose and D-galactose, present on the cell surface. The dye rhodamine and two InP/ZnS quantum dots (QDs) emitting in the green and in the red regions were used as fluorescent probes. Rhodamine-MIPGlcA and rhodamine-MIPNANA were synthesized as monodispersed 400 nm sized particles and were found to bind selectively their targets located in the extracellular region, as imaged by epifluorescence and confocal microscopy. In contrast, when MIP-GlcA and MIP-NANA particles with a smaller size (125 nm) were used, the MIPs being synthesized as thin shells around green and red emitting QDs respectively, it was possible to stain the intracellular and pericellular regions as well. In addition, simultaneous dual-color imaging with the two different colored QDs-MIPs was demonstrated. Importantly, the MIPs were not cytotoxic and did not affect cell viability; neither was the cells morphology affected as demonstrated by live cell imaging. These synthetic receptors could offer a new and promising imaging tool to monitor disease progression.

Chia Gomez L. P., Spangenberg A., Ton X. A., Fuchs Y., Bokeloh F., Malval J. P., Tse Sum Bui B., Thuau D., Ayela C., Haupt K. & Soppera O. Rapid prototyping of chemical microsensors based on molecularly imprinted polymers synthesized by two-photon stereolithography.. Adv. Mater., 2016, 28,5931-5937.
 

Beyazit S., Tse Sum Bui B., Haupt K. & Gonzato C. Molecularly imprinted polymer nanomaterials and nanocomposites by controlled/living radical polymerization.. Prog. Polym. Sci., 2016, 62,1-21.
 

Parlak O., Beyazit S., Tse Sum Bui B., Haupt K., Turner A. P. F. & Tiwari A. Programmable bioelectronics in a stimuli-encoded 3D graphene interface.. Nanoscale, 2016, 8,9976-9981.
 

Xu J. J., Ambrosini S., Tamahkar E., Rossi C., Haupt K. & Tse Sum Bui B. Toward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins.. Biomacromolecules, 2016, 17,345-353.
 

Nestora S., Merlier F., Beyazit S., Prost E., Duma L., Baril B., Graves A., Haupt K. & Tse Sum Bui B. Plastic antibodies for cosmetics: Molecularly imprinted polymers scavenge precursors of malodors. . Angew. Chem. Int. Ed., 2016, 55,6252-6256.
 

Panagiotopoulou M., Salinas Y., Beyazit S., Kunath S., Mayes A. G., Duma L., Prost E., Resmini M., Tse Sum Bui B. & Haupt K. Molecularly imprinted polymer-coated quantum dots for multiplexed cell targeting and imaging.. Angew. Chem. Int. Ed., 2016, 55,8244-8248.
 

Parlak O., Beyazit S., Jafari M. J., Tse Sum Bui B., Haupt K., Tiwari A. & Turner A. P. F. Light-triggered switchable graphene-polymer hybrid bioelectronics.. Adv. Mater. Interfaces, 2016, 3,1500353.
 

Nestora S., Merlier F., Prost E., Haupt K., Rossi C. & Tse Sum Bui B. Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer.. J. Chromatogr. A, 2016, 1465,47-54.
 

Foguel M. V., Ton X. A., Zanoni M. V. B., Sotomayor M. D. P. T., Haupt K. & Tse Sum Bui B. A molecularly imprinted polymer-based evanescent wave fiber optic sensor for the detection of basic red 9 dye. . Sens. Actuators B, 2015, 218,222-228.
 

Panagiotopoulou M., Beyazit S., Nestora S., Haupt K., & Tse Sum Bui B. Initiator-free synthesis of molecularly imprinted polymers by polymerization of self-initiated monomers.. Polymer, 2015, 66,43-51.
 

Ton X. A., Acha V., Bonomi P., Tse Sum Bui B. & Haupt K. A disposable evanescent wave fiber optic sensor coated with a molecularly imprinted polymer as a selective fluorescent probe.. Biosens. Bioelectron., 2015, 64,359-366.
 

Adali-kaya Z., Tse Sum Bui B., Falcimaigne-cordin A. & Haupt K. Molecularly imprinted polymer nanomaterials and nanocomposites: atom-transfer radical polymerization with acidic monomers.. Angew. Chem. Int. Ed., 2015, 54,192-195.
 

Beyazit S., Ambrosini S., Marchyk N., Palo E., Kale V., Soukka T., Tse Sum Bui B. & Haupt K. Versatile synthetic strategy for coating upconverting nanoparticles with polymer shells through localized photopolymerization by using the particles as internal light sources.. Angew. Chem. Int. Ed., 2014, 53,8919-8923.
 

Li B., Xu J., Hall A. J., Haupt K. & Tse Sum Bui B. Water-compatible silica sol-gel molecularly imprinted polymer as potential delivery system for the controlled release of salicylic acid.. J. Mol. Recognit., 2014, 27,559-565.
 

Marchyk N., Maximilien J., Beyazit S., Haupt K. & Tse Sum Bui B. One-pot synthesis of iniferter-bound polystyrene core nanoparticles for the controlled grafting of multilayer shells.. Nanoscale, 2014, 6,2872-2878.
 

Cakir P., Cutivet A., Resmini M., Tse Sum Bui B. & Haupt K. Protein-size molecularly imprinted polymer nanogels as synthetic antibodies, by localized polymerization with multi-initiators.. Adv. Mater., 2013, 25,1048-1051.
 

Ton X. A., Tse Sum Bui B., Resmini M., Bonomi P., Dika I., Soppera O. & Haupt K. A versatile fiber-optic fluorescence sensor based on molecularly imprinted microstructures polymerized in situ.. Angew. Chem. Int. Ed., 2013, 52,8317-8321.
 

Ambrosini S., Beyazit S., Haupt K. & Tse Sum Bui B. Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition.. Chem. Commun., 2013, 49,6746-6748.
 

Haupt K., Linares A. V., Bompart M. & Tse Sum Bui B. Molecularly imprinted polymers.. Top. Curr. Chem., 2012, 325,1-28.
 

Ton X. A., Acha V., Haupt K. & Tse Sum Bui B. Direct fluorimetric sensing of UV-excited analytes in biological and environmental samples using molecularly imprinted polymer nanoparticles and fluorescence polarization.. Biosens. Bioelectron., 2012, 36,22-28.
 

Harz S., Shimmelpfennig M., Tse Sum Bui B., Marchyk N., Haupt K. & Feller K. H. Fluorescence optical spectrally resolved sensor based on molecularly imprinted polymers and microfluidics.. Eng. Life Sci., 2011, 11,559-565.
 

Tse Sum Bui B. & Haupt K. Preparation and evaluation of a molecularly imprinted polymer for the selective recognition of testosterone – application to molecularly imprinted sorbent assays.. J. Mol. Recognit., 2011, 24,1123-1129.
 

Piperno S., Tse Sum Bui B., Haupt K. & Ghebert L. A. Immobilization of molecularly imprinted polymer nanoparticles in electrospun poly(vinyl alcohol) nanofibers.. Langmuir, 2011, 27,1547-1550.
 

Tse Sum Bui B. & Haupt K. Molecularly imprinted polymers : synthetic receptors in bioanalysis. Anal. Bioanal. Chem., 2010, 398,2481-2492.
 

Tse Sum Bui B., Merlier F. & Haupt K. Towards the use of a molecularly imprinted polymer in doping analysis :selective preconcentration and analysis of testosterone and epitestosterone in human urine.. Anal. Chem., 2010, 82,4420-4427.
 

Lotierzo M., Tse Sum Bui B., Leech H. K., Warren M. J., Marquet A. & Rigby S. E. J. Iron-sulfur cluster dynamics in biotin synthase: A new [2Fe-2S]1+ cluster.. Biochem. Biophys. Res. Commun. , 2009, 381,487-490.
 

Mhaka B., Cukrowska E., Tse Sum Bui B., Ramstrom O., Haupt K., Tutu H. & Chimuka L. Selective extraction of triazine herbicide from food samples based on a combination of a liquid membrane and molecularly imprinted polymers.. J. Chromatogr. A, 2009, 1216,6796-6801.
 

Tse Sum Bui B., Belmont A. S., Witters H. & Haupt K. Molecular recognition of endocrine disruptors by synthetic and natural 17beta-estradiol receptors: a comparative study.. Anal. Bioanal. Chem., 2008, 390,2081-2088.
 

BOOK

Xu J, Medina-rangel P. X., Haupt K. & Tse Sum Bui B. Guide to the preparation of molecularly imprinted polymer nanoparticles for protein recognition, by solid-phase synthesis. Methods Enzymol, 2017, ,pp.115-141
  

Panagiotopoulou M., Kunath S., Haupt K., Tse Sum Bui B. Cell and Tissue Imaging with Molecularly Imprinted Polymers . Methods in Molecular Biology, 2017, ,PP.399-415
  

Maximilien J., Beyazit S., Rossi C., Haupt K. & Tse Sum Bui B Nanoparticles in biomedical applications. Measuring Biological impacts of nanomaterials, 2016, Springer International Publishing, Switzerland,pp. 177-210
  

Sébastien ACKET

Manager Analytical Platform

          Contact Sébastien Acket

 

Research

Member of the theme “Plant Metabolism and Bioresources”.

Understanding of lipid synthesis, regulation and accumulation mechanisms in oleaginous field crop cells and in reviviscent plants able to withstand extreme weather conditions.
Skills

Plant metabolism, lipidomics and non-target metabolomics, isotopic profiling, fluxomics (MFA, INST-MFA, FBA).
Roles and responsabilities

In charge of the Bioanalysis platform, UMR CNRS 7025.

Development of analytical methods in mass spectrometry for fluxomics, metabolomics and non-targeted lipidomics.

Member of the French network of metabolomics and fluxomics (http://www.rfmf.fr/).

Member of the network of lipidomysts (http://lipidomystes.gerli.com/reseau/).

 

General information

  • Since 2016: Engineer, UMR CNRS 7025 Génie enzymatique et Cellulaire, Compiègne.
  • 2015-2016: Engineer on the Metabolomic and Fluxomic Platform, UMR INRA 1332 Biologie du Fruit et Pathologie, Villenave d’ornon.
  • 2011-2015: PhD “Involvement of carbon metabolism for differential oil production in oleaginous-linseed plants: modeling the systems” UMR CNRS 7025 Génie enzymatique et Cellulaire, Compiègne.

 

Personal interests

Badminton, music (Le dualo), gardening and organic farming, traveling.

 

 

Articles

Acket S., Degournay A., Rossez Y., Mottelet S., Villon P., Troncoso-ponce A., Thomasset B. 13C-Metabolic Flux Analysis in Developing Flax (Linum usitatissinum L.) Embryos to Understand Storage Lipid Biosynthesis . Metabolites , 2020, 10,14.
 

Martins-noguerol R., Moreno-perez A. J., Acket S., Troncoso-ponce A., Garces R., Thomasset B., Salas J. J. Et Martinez Force E. Impact of sunflower (Helianthus annuusL.) plastidial lipoyl synthases genes expression in glycerolipids of transgenic Arabidopsis plants . Scientific Reports , 2020, 10 ,3749-3764 .
 

Martins-noguerol R., Moreno-pérez Aj., Acket S., Makni S., Garcés R., Troncoso-ponce A., Salas Jj., Thomasset B., Martínez-force E. Lipidomic Analysis of Plastidial Octanoyltransferase Mutants of Arabidopsis thaliana . Metabolites , 2019, 9(10) ,209 .
 

Bernard C., Acket S., Rossez Y ., Fernandez O., Berton T., Gibon Y., Cabasson C. Untargeted analysis of semipolar compounds by LC-MS and targeted analysis of fatty acids by GC-MS/GC-FID: From plant cultivation to extract preparation. Methods Molec. Biol., 2018, 42,20171-20179.
     ABSTRACT: The way plants are grown and samples are harvested, prepared, and extracted has a profound impact on the output of a metabolomics experiment. In this chapter, we detail the experimental procedures from plant cultivation to extract preparation, in order to avoid difficulties that could result in contamination or undesired changes of the analytes. Two plant organs are mentioned as examples: tomato fruits (Solanum lycopersicum) and flax seeds (Linum usitatissimum). Extractions designed for the untargeted analysis of semipolar compounds by liquid chromatography

Acket S., Degournay A., Gosset M., Merlier F., Troncoso-ponce M. A. & Thomasset B. Analysis of C-13 labeling amino acids by capillary electrophoresis - High resolution mass spectrometry in developing flaxseed.. Anal. Biochem., 2018, 547,14-18.
     ABSTRACT: n context of fluxomic studies, 13C labeling analysis of amino acids are very important for solving the carbon flow calculation, because they are synthesized in various biosynthesis pathways and cellular compartments in plant cells. Traditionally, 13C labeling analysis are performed using low resolution mass spectrometry detector by GC-MS. We compared a method using capillary electrophoresis-high resolution mass spectrometry without derivatization and with better accuracy assessment of labeling measurements comparing to classical GC-MS. Our method allowed us to show that valine, leucine, alanine are not synthesized from the same pyruvate pool during the period of reserves accumulation in flax seeds.

Tshabuse F., Farrant J. M., Humbert L., Moura D., Rainteau D., Espinasse C., Idrissi A., Merlier F., Acket S., Rafudeen M. S., Thomasset B. & Ruelland E. Glycerolipid analysis during desication and recovery of the resurrection plant Xerophyta humilis (Bak) Dur and Schinz.. Plant Cell Environ., 2018, 41,533-547.
     ABSTRACT: Feelings in humans are mental states representing groups of physiological functions that usually have defined behavioural purposes. Feelings, being evolutionarily ancient, are thought to be coordinated in the brain stem of animals. One function of the brain is to prioritise between competing mental states and, thus, groups of physiological functions and in turn behaviour. Plants use groups of coordinated physiological activities to deal with defined environmental situations but currently have no known mental state to prioritise any order of response. Plants do have a nervous system based on action potentials transmitted along phloem conduits but which in addition, through anastomoses and other cross?links, forms a complex network. The emergent potential for this excitable network to form a mental state is unknown, but it might be used to distinguish between different and even contradictory signals to the individual plant and thus determine a priority of response. This plant nervous system stretches throughout the whole plant providing the potential for assessment in all parts and commensurate with its self?organising, phenotypically plastic behaviour. Plasticity may, in turn, depend heavily on the instructive capabilities of local bioelectric fields enabling both a degree of behavioural independence but influenced by the condition of the whole plant.

Acket S., Degournay A., Merlier F. & Thomasset B. Data documenting the comparison between the theoretically expected values of free sugars mass isotopomer composition with standards using GC-MS and LC-HRMS for Metabolic Flux Analysis.. Data in Brief, 2017, 12,108-112.
     ABSTRACT: The data presented in this article are related to the research article entitled

Acket S., Degournay A., Merlier F. & Thomasset B. 13C labeling analysis of sugars by hight resolution-mass spectrometry for metabolic flux analysis.. Anal. Biochem., 2017, 527,45-48.
     ABSTRACT: Metabolic flux analysis is particularly complex in plant cells because of highly compartmented metabolism. Analysis of free sugars is interesting because it provides data to define fluxes around hexose, pentose, and triose phosphate pools in different compartment. In this work, we present a method to analyze the isotopomer distribution of free sugars labeled with carbon 13 using a liquid chromatography