Researchers

Bérangère BIHAN-AVALLE

PR UTC - Co-Animator Theme BBD

    Contact Bérangère Bihan-Avalle

 

Articles

Xu J., Merlier F., Avalle B., Veillard V., Debré P., Haupt K., Tse Sum Bui B. Molecularly Imprinted Polymer Nanoparticles as Potential Synthetic Antibodies for Immunoprotection against HIV . ACS Appl. Mater. Interfaces2019, 11,9824-9831.
     ABSTRACT: We describe the preparation and characterization of synthetic antibodies based on molecularly imprinted polymer nanoparticles (MIP-NPs) for the recognition and binding of the highly conserved and specific peptide motif SWSNKS (3S), an epitope of the envelope glycoprotein 41 (gp41) of human immunodeficiency virus type 1 (HIV-1). This motif is implicated in the decline of CD4+ T cells and leads to the deterioration of the immune system during HIV infection. Therefore, the development of MIP-NPs that can target and block the 3S peptide to prevent subsequent cascade interactions directed toward the killing of CD4+ T cells is of prime importance. Because most antibodies recognize their protein antigen via a conformational or structured epitope (as opposed to a linear epitope commonly used for molecular imprinting), we employed protein molecular modeling to design our template epitope so that it mimics the three-dimensional structure fold of 3S in gp41. The resulting template peptide corresponds to a cyclic structure composed of CGSWSNKSC, with the 3S motif well orientated for imprinting. MIP-NPs with a size of 65 nm were obtained by solid-phase synthesis and were water-soluble. They were prepared by a judicious combination of multiple functional monomers affording hydrogen bonding, ionic, π–π, and hydrophobic interactions, conferring high affinity and selectivity toward both the cyclic peptide and the whole gp41 protein. These results suggest that our MIPs could potentially be used for blocking the function of the 3S motif on the virus.

Shahsavarian M., Chaaya N., Costa N., Boquet D., Atkinson A., Offmann B., Kaveri Sv., Lacroix-desmazes S., Friboulet A., Avalle B. & Padiolleau-lefevre S. Multi-target selection of catalytic antibodies wih . FEBS J.2017, 284,634-653.
     ABSTRACT: ??lactamase enzymes responsible for bacterial resistance to antibiotics are among the most important health threats to the human population today. Understanding the increasingly vast structural motifs responsible for the catalytic mechanism of ??lactamases will help improve the future design of new generation antibiotics and mechanism?based inhibitors of these enzymes. Here we report the construction of a large murine single chain fragment variable (scFv) phage display library of size 2.7 ? 109 with extended diversity by combining different mouse models. We have used two molecularly different inhibitors of the R?TEM ??lactamase as targets for selection of catalytic antibodies with ??lactamase activity. This novel methodology has led to the isolation of five antibody fragments, which are all capable of hydrolyzing the ??lactam ring. Structural modeling of the selected scFv has revealed the presence of different motifs in each of the antibody fragments potentially responsible for their catalytic activity. Our results confirm (a) the validity of using our two target inhibitors for the in vitro selection of catalytic antibodies endowed with ??lactamase activity, and (b) the plasticity of the ??lactamase active site responsible for the wide resistance of these enzymes to clinically available inhibitors and antibiotics.

Padiolleau-lefevre S., Ben Naya R., Shahsavarian M., Friboulet A. & Avalle B. Catalytic antibodies and their applications in biotechnology: State of the art.. Biotechnol. Lett.2014, 36,1369-1379.
 

Shahsavarian M., Le Minoux D., Matti K. M., Kaveri S., Lacroix-desmazes S., Boquet D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Exploitation of rolling circle amplification for the construction of large phafe display antibody libraries.. J. Immunol. Methods2014, 407,26-34.
 

Ben Naya R., Matti K., Guellier A., Matagne A., Boquet D., Thomas D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Efficient refolding of a recombinant abzyme: Structural and catalytic characterizations.. Appl. Microbiol. Biotechnol.2013, 97,7721-7731.
 

Rosant C., Avalle B., Larcher D., Dupont A., Friboulet A. & Tarascon J. M. Biosynthesis of electrode nanomaterials by phage engineering: comprehension and future.. Energ. Environ. Sci.2012, 5,9936-9943.
 

Le Minoux D., Mahendra A., Lacroix-desmazes S., Limnios N., Friboulet A., Avalle B., Boquet D., Kaveri S. & Padiolleau-lefevre S. A novel molecular analysis of genes encoding catalytic antibodies.. Mol. Immunol.2012, 50,160-168.
 

Gabibov A. G., Belogurov A. A., Lomakin Y. A., Zakharova M. Y., Avakyan M. E., Dubrovskaya V. V., Smirnov I. V., Ivanov A. S., Molnar A. A., Gurtsevitch V. E., Diduk S. V., Smirnova K. V., Avalle B., Sharanova S. N., Tramontano A., Friboulet A., Boyko A. N., Ponomarenko N. A. & Tikumova N. V. Combinatorial antibody library from Multiple Sclerosis patients reveals antibodies that cross-react with myelin basic protein and EBV-antigen.. FASEB J.2011, 25,4211-4221.
 

Smirnov I., Carletti E., Kurkova I., Nachon F., Nicolet Y., Mitkevich V., Debat H., Avalle B., Belogurov A., Kuznetsov N., Reshetnyak A., Masson P., Tonevitsky A., Ponomarenko N., Makarov A. A., Friboulet A., Tramontano A. & Gabibov A. G. Reactibodies generated by kinetic selection couple chemical reactivity with faborable protein dynamics.. Proc. Natl. Acad. Sci. USA2011, 108,15954-1959.
 

Phichith D., Bun S., Padiolleau-lefevre S., Guellier A., Banh S., Gallieni M., Frere J. M., Thomas D., Friboulet A. & Avalle B. Novel peptide inhibiting both TEM-1 beta-lactamase and penicillin-binding proteins.. FEBS J.2010, 277,4965-4972.
 

Phichith D., Bun S., Padiolleau-lefevre S., Banh S., Thomas D., Friboulet A. & Avalle B. Mutational and inhibitory analysis of a catalytic antibody. Implication for drug discovery.. Mol. Immunol.2009, 47,348-356.
 

Smirnov I. V., Vorobiev I. I., Friboulet A., Avalle B., Thomas D., Knorre V. D., Gabibov A. G. & Ponomarenko N. A. The antiidiotypic approach to obtaining a proteolytic antibody.. Dokl. Biochem. Biophys.2008, 420,105-107.
 

Belogurov A. A., Kurkova I. N., Friboulet A., Thomas D., Misikov V. K., Zakharova M. U., Suchkov S. V., Kotov V., Alexin A. I., Avalle B., Souslova E. A., Morse H. C., Gabibov A. G. & Ponomarenko N. A. Recognition and Degradation of Myelin Basic Protein Peptides by Serum Autoantibodies as a Novel Biomarker for Multiple Sclerosis.. J. Immunol.2008, 180,1258-1267.
 

Ponomarenko N. A., Pillet D., Paon M., Vorobiev I. I., Smirnov I. V., Adenier H., Avalle B., Kolesnikov A. V., Kozyr A. V., Thomas D., Gabibov A. G. & Friboulet A. Anti-idiotypic antibody mimics proteolytic function of parent antigen.. Biochemistry 2007, 46,14598-14609.
 

Ponomarenko N. A., Vorobiev I. I., Alexandrova E. S., Reshetnyak A. V., Telegin G. B., Khaidurov S. V., Avalle B., Karavanov A., Morse H. C., Thomas D., Friboulet A. & Gabibov A. G. Induction of protein-targeted catalytic response in autoimmune prone mice: antibody-mediated cleavage of HIV-1 glycoprotein gp120. . Biochemistry2006, 45,324-330.
 

Padiolleau-lefevre S., Debat H., Phichith D., Thomas D., Friboulet A. & Avalle B. Expression of a functional scFv fragment of an anti-idiotypic antibody with a ß-lactam hydrolytic activity. . Immunol. Letters2006, 103,39-44.
 

Ponomarenko N. A., Durova O. M., Vorobiev I. I., Belogurov A. A., Telegin G. B., Suchkov S. V., Misikov V. K., Kiselev S. L., Lagarkova M. A., Govorun V. M., Serebryakova M. V., Avalle B., Tornatore P., Karavanov A., Morse H. C., Thomas D., Friboulet A. & Gabibov A. G. Autoantibodies to myelin basic protein catalyze site specific degradation of their antigen. . Proc. Natl. Acad. Sci. USA2006, 103,281-286.
 

Patent

B. Avalle-bihan, A. Friboulet, H. Isber, C. Loussouarn, S. Padiolleau-lefèvre. Inhibiteurs de STAT5 et utilisation de ceux-ci. 2014, ,.
 

A. Friboulet, B. Avalle-bihan, H. Débat, D. Thomas Composés capables de moduler l'activité et de stimuler la production d'un anticorps catalytique. France, n° 01067542001, ,.
 

Sébastien BUCHOUX

MC UPJV

      Contact Sébastien Buchoux

 

 

Articles

Monnier N., Furlan A-l., Buchoux S., Deleu M., Dauchez M., Rippa S., Sarazin C. Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies" has been published in IJMS as part of the Special Issue SAR "Out of the Box. Int. J. Mol. Sci.2019, 20,1009.
 

Buchoux S. FATSLiM: a fast and robust software to analyze MD simulations of membranes.. Bioinformatics2017, 33,133-154.
 

Furlan A., Jobin M. L., Buchoux S., Grelard A., Dufourc E. & Gean J. Membrane lipids protected from oxidation by red wine tannins: a proton NMR study.. Biochimie2014, 107,82-90.
 

Auxenfans T., Buchoux S., Husson E. & Sarazin C. Efficient enzymatic saccharification of Miscanthus: energy-saving by combinig dilute acid and ionic liquid pretreatments.. Biomass Bioenerg.2014, 62,82-92.
 

Auxenfans T., Buchoux S., Larcher T., Husson G., Husson E. & Sarazin C. Enzymatic saccharification and structural properties of industrial wood sawdust: recycled ionic liquids pretreatments.. Energ. Convers. Manage.2014, 88,1094-1103.
 

Auxenfans T., Buchoux S., Djellab K., Avondo C., Husson E. & Sarazin C. Mild pretreatment and enzymatic saccharification of cellulose with recycled ionic liquid towards one-batch process.. Carbohydr. Polym.2012, 90,805-813.
 

Dufourc E. J., Buchoux S., Toupe J., Sani M. A., Jean-francois F., Khemtemourian L., Grelard A., Loudet_courreges C., Laguerre M., Elezgaray J., Desbat B. & Odaert B. Membrane interacting peptides: from killers to helpers.. Curr. Prot. Pept. Sci.2012, 13,620-631.
 

Husson E., Buchoux S., Avondo C., Cailleu D., Djellab K., Gosselin I., Wattraint O. & Sarazin C. Enzymatic hydrolysis of ionic liquid-pretreated celluloses: contribution of CP-MAS, 13C NMR and SEM.. Bioresource Technol.2011, 102,7335-7342.
 

Zhendre V., Grelard A., Garnier-lhomme M., Buchoux S., Larijani B. & Dufourc E. J. Key role of polyphosphoinositides in dynamics of fusogenic nuclear membrane vesicles.. PloS One2011, 6,e23859.
 

Batchelor R., Windle C. J., Buchoux S. & Lorch M. Cholesterol and lipid phases influence the interactions between serotonin receptor agonists and lipid bilayers.. J. Biol. Chem.2010, 285,41402-41411.
 

Aude CORDIN

MC UTC

Contact Aude Cordin

 

Research

Member of the theme “Biomimicry and Biomolecular Diversity”.

Development of molecular imprinted polymers for applications in biosensors and separation sciences.

Nanostructured, nanocomposite, bio-based molecularly imprinted polymers and delivery systems for medical, environmental and food application: degradable and biological-responsive materials, study of polymer biodegradation.

Molecular imprinting polymer synthesis by “green chemistry”.

Transversal research activities with the team “plant metabolism and bioressources” in plant biodiversity exploitation and characterization (polyphenols extraction and antioxidants activities).

 

Skills

Teaching activities in chemistry, biochemistry, enzymology and food formulation.

Scientific skills:

  • Molecular imprinting technology: polymerization methods (FRP, ATRP, precipitation polymerization, enzymatic polymerization…), surface functionalization, nanostructuration (nanomolding, nanocomposites, core-shell particles…).
  • Biological-responsive polymers synthesis, degradation and release properties studies.
  • Biocatalyse, biopolymer and natural products modification.
  • Encapsulation methods: emulsion, ionic-gelation, natural crosslinking, sol-gel.
  • Antioxidant activities

 

Roles and responsibilities

In charge of assistant engineer internships in biotechnology at University of Technology of Compiègne.

 

General information

  • Since 2006 : Lecturer at Université de Technologie de Compiègne in the Laboratory of Enzyme and Cell Engineering (UMR CNRS 7025)
  • 2005 : Post-doctoral position at Ecole Nationale Supérieure de Chimie de Montpellier, Laboratoire des Matériaux Catalytiques et Catalyse en Chimie Organique, Montpellier, France. “Protein immobilization and encapsulation in nanostructured silica materials for wastewater treatment”.
  • 2004: PhD in Biotechnology and Food Process, at Institut National Polytechnique de Lorraine (INPL, ENSAIA, Nancy, France), PhD thesis on: “Development of an enzymatic process for the production of flavonoid esters : kinetic, purification, conformational study, biological and physic-chemical properties.”
  • 2001: Master in organic chemistry, Paris-Sud XI University. “Bienzymatic system for asymmetric sulfoxidation”.
  • 2000: Engineer degree in organic chemistry, Ecole Nationale Supérieure de Chimie de Rennes (ENSCR), France

 

Articles

Adali-kaya Z., Tse Sum Bui B., Falcimaigne-cordin A. & Haupt K. Molecularly imprinted polymer nanomaterials and nanocomposites: atom-transfer radical polymerization with acidic monomers.. Angew. Chem. Int. Ed.2015, 54,192-195.
 

Zdunek J., Benito-pena E., Linares A., Falcimaigne-cordin A., Orellana G., Haupt K. & Moreno-bondi M. C. Surface-imprinted nanofilaments for Europium-amplified luminescent detection of fluoroquinolones antibiotics.. Chem.-Eur. J.2013, 19,10209-10216.
 

Boussetta N., Vorobiev E., Le L. H., Cordin-falcimaigne A. & Lanoiselle J. L. Application of electrical treatments in alcoholic solvent for polyphenols extraction from grape seeds.. LWT-Food Sci. Technol.2012, 46,127-134.
 

Biffis A., Dvorakova G. & Falcimaigne-cordin A. Physical forms of MIPS.. Top. Curr. Chem.2012, 325,29-82.
 

Boussetta N., Vorobiev E., Deloison V., Pochez F., Falcimaigne-cordin A. & Lanoiselle J. L. Valorisation of grape pomace by the extraction of phenolic antioxidants: Application of high voltage electrical discharges.. Food Chem.2011, 128,364-370.
 

Linares A. V., Falcimaigne-cordin A., Gheber L. A. & Haupt K. Patterning nanostructured, synthetic, polymeric receptors by simultaneous projection photolithography, nanomolding, and molecular imprinting.. Small2011, 7,2318-2325.
 

Linares A. V., Vandevelde F., Pantigny J., Falcimaigne-cordin A. & Haupt K. Polymer films composed of surface-bound nanofilaments with a high aspect ratio, molecularly imprinted with small molecules and proteins.. Adv. Funct. Mater.2009, 19,1299-1303.
 

Nicola D’AMELIO

PR UPJV

        Contact Nicola D’Amelio

 

 

Research

I’m working at the interface between the theme “Plant Metabolism and Bioresources” and “Biomimicry and Biomolecular Recognition”.

Main research topics:

  • Structure and dynamics of biomolecules involved in cellular processes or pathologies: Antimicrobial and anticancer peptides interacting with membrane models, antibiotic effects on bacterial protein expression, proteins involved in cancer (kinases, matrix metalloprotinases)  and neurodegenerative disorders (a-synuclein, superoxide dismutase, WW domains), photosynthesis inhibition, and oxidative stress caused by metal ions.
  • ADAPTABLE web-server development (http://gec.u-picardie.fr/adaptable/index.html). Anticancer and Antimicrobial peptide alignment and clustering.

 

Skills

Multidimensional NMR, biomolecular interactions, protein structure and dynamics,  paramagnetic probes, quadrupolar nuclei, relaxation dispersion, bioinformatics.

 

Roles and responsabilities

Supervisor of two PhD students.

Teaching in Structural Biology, NMR, Biochemistry, Enzymology and Food science at bachelor and master levels.

 

General information

Education and work experience:

  • 2016: Professorship in Biochemistry at GEC, UPJV (Université de Picardie Jules Verne), Amiens, France
  • 2014-2016: Senior research associate, IMDEA (Institutos Madrileños of Estudios Avanzados)/ CNB (National Center Biotechnology), Madrid, Spain
  • 2013-2014: Senior researcher, Computational Biophysics Group, UCL (University College London) 2011-2013 Staff scientist, Computational Biophysics Group, Spanish National Institute for Cancer Research, CNIO (Centro Nacional Investigaciones Oncológicas), Madrid, Spain
  • 2007-2011: Research scientist, Bracco Imaging pharma, Research Center Trieste, Area Science Park, Italy
  • 2004-2007: Postdoctoral Research fellow, CERM (Centro Risonanze Magnetiche), Florence Italy
  • 2000-2004: Postdoctoral Research fellow, Chemistry Department, University of Siena, Siena, Italy
  • 1999: Chemical Sciences. Ph.D.: “The folding pattern of cytochrome b562: the relevance of NMR and dynamic studies on the apo-protein”, (work developed in Robert Kaptein’s lab at Utrecht University, The Netherlands), University of Perugia, Italy

Personal interests

Painting (www.nicoladamelio.com)

 

 

 

Articles

 

Luminita DUMA

CR CNRS - Co-Animator BBD

               Contact Luminita Duma

 

Research
Member of the theme “Biomimicry and Biomolecular Diversity”.

My research activities focus on molecular recognition in biomolecules and molecularly imprinted polymers. On the first part, I use my skills in solid-state and solution NMR in a collaborative project with Jean-Jacques Lacapere (LBM, UPMC, Paris) on the membrane protein TSPO (translocator protein) to extract structural information in order to help the understanding of the structure-dynamics-function relationship. On the second part, which I initiated in 2015, I aim to better understand the interaction mechanisms responsible for the formation of the molecular imprint in the polymer matrix and the factors responsible for the molecular recognition of an analyte by a molecularly imprinted polymer thanks to different approaches (NMR spectroscopy, calorimetry and molecular modelling); this should make possible the development of a generic method for the rational development of these polymers.

Skills
Nuclear magnetic resonance, isothermal calorimetry, molecular modelling, molecular recognition, biomolecules, molecularly imprinted polymers.
Roles and responsabilities
Co-animateur theme BDM, Member of the website editorial team, Member of the “Magnetism and Magnetic Resonances” group at the DCP (Division Chimie Physique) of SCF (Société de Chimie Francaise).

General information

  • Since July 2015: CNRS researcher, Enzyme and Cell Engineering Laboratory, Compiègne University of Technology, France
  • 2008 – 2015: CNRS researcher, Chemistry Department, ENS, France
  • 2006 – 2008: Post-doc at Ecole Normale Supérieure (ENS), Paris, France. “Broadband dipolar recoupling in solid-state NMR spectroscopy”
  • 2004 – 2006: Post-doc at Biozentrum, University of Basel, Switzerland. “NMR study of the interaction between the chemokine RANTES and HIV-1 CCR5-derived peptides”
  • May – July 2003: Marie Curie Fellow at CERM of University of Firenze: “Direct carbon detection for diamagnetic and paramagnetic proteins”
  • 2001 – 2004: PhD, ENS-Lyon, France, Chemistry Department. PhD thesis on “Spin-state-selective techniques for resolution improvement in solid and liquid-state NMR spectroscopy

 

Personal interests
Music, literature, mountaineering, hiking and climbing.

 

 

Articles

Panagiotopoulou M., Salinas Y., Beyazit S., Kunath S., Mayes A. G., Duma L., Prost E., Resmini M., Tse Sum Bui B. & Haupt K. Molecularly imprinted polymer-coated quantum dots for multiplexed cell targeting and imaging.. Angew. Chem. Int. Ed.2016, 55,8244-8248.
 

Nestora S., Merlier F., Beyazit S., Prost E., Duma L., Baril B., Graves A., Haupt K. & Tse Sum Bui B. Plastic antibodies for cosmetics: Molecularly imprinted polymers scavenge precursors of malodors. . Angew. Chem. Int. Ed.2016, 55,6252-6256.
 

Chan-huot M., Wimperis S., Gervais C., Bodenhausen G., Duma L. Deuterium MAS NMR studies of dynamics on multiple timescales: histidine and oxalic acid examples. ChemPhysChem2015, 16,204-215
  

Sehgal A., Duma L., Bodenhausen G., Pelupessy P. Fast proton exchange in histidine: measurement of rate constants through indirect detection by NMR spectroscopy. Chem. Eur. J.2014, 20,6332-6338
  

Caillon L., Duma L., Lequin O., Khemtemourian L. Cholesterol modulates the interaction of the islet amyloid polypeptide with membranes. Mol. Membr. Biol.2014, 31,239-249
  

Chan-huot M., Lesot P., Pelupessy P., Duma L., Bodenhausen G., Duchambon P., Toney M. D., Reddy U. V., Suryaprakash N. On-the-fly kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media. Anal. Chem.2013, 85,4694-4697
  

Chan-huot M., Duma L., Charbonnier J. -b., Herbert-pucheta J. -e., Assairi L., Blouquit Y., Abergel D., Bodenhausen G. Large-scale production of microcrystals and precipitates of proteins and their complexes. Cryst. Growth Des.2012, 12,6199-6207
  

Herbert-pucheta J. -e., Chan-huot M., Duma L., Abergel D., Bodenhausen G., Assairi L., Blouquit Y., Tekely P. Probing structural and motional features of C-terminal part of the human centrin 2/P17-XPC microcrystalline complex by solid-state NMR spectroscopy. J. Phys. Chem. B2012, 116,14581-14591
  

Folliet N., Roiland C., Begu S., Aubert A., Minerva T., Goursot A., Selvaraj K., Duma L., Tielens F., Mauri F., Laurent G., Bonhomme C., Gervais C., Babonneau F., AzaÏs T. Investigation of the interface in silica-encapsulated liposomes by combining solid state and NMR first principles calculations. J. Am. Chem. Soc.2011, 133,16815-16827
  

Paquin R., Pelupessy P., Duma L., Gervais C., Bodenhausen G. Determination of the antisymmetric part of the chemical shift anisotropy tensor via spin relaxation in nuclear magnetic resonance. J. Chem. Phys.2010, 133,034506-034516
  

Segawa T., Kateb F., Duma L., Bodenhausen G., Pelupessy P. Exchange rate constants of invisible protons in proteins determined by NMR spectroscopy. ChemBioChem2008, 9,537-542
  

Duma L., Abergel D., Tekely P., Bodenhausen G. Proton chemical shift anisotropy measurements of hydrogen bonded functional groups by fast magic-angle spinning solid-state NMR spectroscopy. Chem. Comm.2008, *,2361-2363
  

Duma L., Abergel D., Ferrage F., Pelupessy P., Tekely P., Bodenhausen G. Broadband dipolar recoupling for magnetization transfer in solid-state NMR correlation spectroscopy. ChemPhysChem2008, 9,1104-1106
  

Pelupessy P., Duma L., Bodenhausen G. Improving resolution in single-scan 2D spectroscopy. J. Magn. Reson.2008, 194,169-174
  

Weingarth M., Raoua N., Jouvelet B., Duma L., Bodenhausen G., Boujlel K., Schollhorn B. Revealing molecular self-assembly and geometry of non-covalent halogen bonding by solid-state NMR spectroscopy. Chem. Comm.2008, *,5981-5983
  

Duma L., HÄussinger D., Rogowski M., Lusso P., Grzesiek S. Binding surface of RANTES chemokine complexed with CCR5-derived peptides. J. Mol. Biol.2007, 365,1063-1075
  

Cadars S., Sein J., Duma L., Lesage A., Pham T. N., Brown S. P., Baltisberger J. H., Emsley L. The refocused INADEQUATE MAS NMR experiment in multiple spin-systems: Interpreting observed correlation peaks and optimising lineshapes. J. Magn. Reson.2007, 188,24-34
  

Lai W. -c., Mclean N., Gansmuller A., Verhoeven M. A., Antonioli G. C., Carravetta M., Duma L., Bovee-geurts P. H. M., Johannessen O. G., De Groot H. J. M., Lugtenburg J., Emsley L., Brown S. P., Brown R. C. D., Degrip W. J., Levitt M. H. Accurate measurements of 13C - 13C J-couplings in the rhodopsin chromophore by double-quantum solid-state NMR. J. Am. Chem. Soc.2006, 128,3878-3879
  

Bermel W., Bertini I., Duma L., Emsley L., Felli I. C., Pierattelli R., Vasos P. R. Complete assignment of heteronuclear protein resonances by protonless NMR. Angew. Chem. Int. Ed.2005, 44,3089-3092
  

Fayon F., Massiot D., Levitt M. H., Titman J. J., Gregory D. H., Duma L., Emsley L., Brown S. P. Through-space contributions to two-dimensional double-quantum J-correlation NMR spectra of magic-angle-spinning solids. J. Chem. Phys.2005, 122,194313-194327
  

Bertini I., Duma L., Felli I. C., Fey M., Luchinat C., Pierattelli R., Vasos P. R. A heteronuclear direct-detection NMR spectroscopy experiment for protein-backbone assignment. Angew. Chem. Int. Ed.2004, 43,2257-2259
  

Duma L., Lai W. -c., Carravetta M., Emsley L., Brown S. P., Levitt M. H. Principles of spin-echo modulation by J-coupling in magic-angle-spinning solid-state NMR. ChemPhysChem2004, 5,815-833
  

Duma L., Hediger S., Lesage A., Sakellariou D., Emsley L. Carbon-13 lineshapes in solid-state NMR of labeled compounds. Effects of coherent CSA-dipolar cross-correlation. J. Magn. Reson.2003, 162,90-101
  

Duma L., Hediger S., Lesage A., Emsley L. Spin-state selection in solid-state NMR. J. Magn. Reson.2003, 164,187-195
  

Duma L., Hediger S., Brutscher B., Bockmann A., Emsley L. Resolution enhancement in multidimensional solid state NMR experiments of 13C-labeled proteins using transition selection. J. Am. Chem. Soc.2003, 125,11816-11817
  

Lesage A., Duma L., Sakellariou D., Emsley L. Improved resolution in proton NMR spectroscopy of powdered solids. J. Am. Chem. Soc.2001, 123,5747-5752
  

POSTER

Senicourt L., Duma L., Papadopoulos V., Lacapere J. -j. Solid-state NMR of membrane protein reconstituted in proteoliposomes. Methods in Molecular Biology2017, *,*
  

Duma L. Nitrogen-14 NMR studies of biological systems. NMR of Quadrupolar Nuclei in Solid Materials, Handbook of Encyclopedia of Magnetic Resonance2012, *,255-272
  

Alain FRIBOULET

DR CNRS

 

     Contact Alain Friboulet

 

 

Main publications

Articles

Shahsavarian M., Chaaya N., Costa N., Boquet D., Atkinson A., Offmann B., Kaveri Sv., Lacroix-desmazes S., Friboulet A., Avalle B. & Padiolleau-lefevre S. Multi-target selection of catalytic antibodies wih . FEBS J.2017, 284,634-653.
     ABSTRACT: ??lactamase enzymes responsible for bacterial resistance to antibiotics are among the most important health threats to the human population today. Understanding the increasingly vast structural motifs responsible for the catalytic mechanism of ??lactamases will help improve the future design of new generation antibiotics and mechanism?based inhibitors of these enzymes. Here we report the construction of a large murine single chain fragment variable (scFv) phage display library of size 2.7 ? 109 with extended diversity by combining different mouse models. We have used two molecularly different inhibitors of the R?TEM ??lactamase as targets for selection of catalytic antibodies with ??lactamase activity. This novel methodology has led to the isolation of five antibody fragments, which are all capable of hydrolyzing the ??lactam ring. Structural modeling of the selected scFv has revealed the presence of different motifs in each of the antibody fragments potentially responsible for their catalytic activity. Our results confirm (a) the validity of using our two target inhibitors for the in vitro selection of catalytic antibodies endowed with ??lactamase activity, and (b) the plasticity of the ??lactamase active site responsible for the wide resistance of these enzymes to clinically available inhibitors and antibiotics.

Saha C., Das M., Stephen-victor E., Friboulet A., Bayry J. & Kaveri S. Differential effect of Viscum album preparations on the maturation and activation of human dendritic cells and CD4+ T cell responses.. Molecules2016, 21,912.
 

Saha C., Hegde P., Friboulet A., Bayry J. & Kaveri S. V. Viscum album-mediated COX-2 inhibition implicates destabilization of COX-2 mRNA.. PloS One2015, 10,e114965.
 

Elluru S. R., Saha C., Hedge P., Friboulet A., Bayry J. & Kaveri S. Dissecting the anti-inflammatory effects of Viscum album: inhibition of cytokine-induced expression of cyclooxygenase-2 and secretion of prostaglandin E2.. Translat. Res. Biomed.2015, 4,67-73.
 

Ponomarenko N., Chatziefthimiou S. D., Kurkova I., Mokrushina Y., Stepanova A., Smirnov I., Avakyan M., Bobik T., Mamedov A., Mitkevich V., Belogurov A., Fedorova O., Dubina M., Golovin A., Lamzin V., Friboulet A., Makarov A., Wilmanns M. & Gabibov A. Role of . Acta Crystallogr. D2014, 70,708-719.
 

Padiolleau-lefevre S., Ben Naya R., Shahsavarian M., Friboulet A. & Avalle B. Catalytic antibodies and their applications in biotechnology: State of the art.. Biotechnol. Lett.2014, 36,1369-1379.
 

Shahsavarian M., Le Minoux D., Matti K. M., Kaveri S., Lacroix-desmazes S., Boquet D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Exploitation of rolling circle amplification for the construction of large phafe display antibody libraries.. J. Immunol. Methods2014, 407,26-34.
 

Sharma M., Lecerf M., Friboulet A., Kaveri S. V., Dissous C. & Bayry J. Dendritic cells but not basophils mediate Th17 responses to Schistosomes.. J. Infect. Dis.2014, 209,2019-2021.
 

Ben Naya R., Matti K., Guellier A., Matagne A., Boquet D., Thomas D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Efficient refolding of a recombinant abzyme: Structural and catalytic characterizations.. Appl. Microbiol. Biotechnol.2013, 97,7721-7731.
 

Smirnov I., Belogurov A., Friboulet A., Masson P., Gabibov A. G. & Renard P. Y. Strategies for the selection of catalytic antibodies against organophosphorus nerve agents.. Chem-Biol. Interact.2013, 203,196-201.
 

Belogurov A. A., Stepanov A. V., Smirnov I. V., Melamed D., Bacon A., Mamedov, A. E., Boltsov V. M., Sashchenko L. P., Ponomarenko N. A., Sharanova S. N., Boyko A. N., Dubina M. V., Friboulet A., Genkin D. D. & Gabibov A. G. Liposome-encapsulated peptides protect against experimental allergic encephalitis.. FASEB J. 2013, 27,222-231.
 

Mahendra A., Peyron I., Dollinger C., Gilardin L., Sharma M., Wootla B., Padiolleau-lefevre S., Friboulet A., Boquet D., Legendre C., Kaveri S., Thaunat O. & Lacroix-desmazes S. IVIg treatment reduces catalytic antibody titers of renal transplanted patients.. PloS One2013, 8,e70731.
 

Rosant C., Avalle B., Larcher D., Dupont A., Friboulet A. & Tarascon J. M. Biosynthesis of electrode nanomaterials by phage engineering: comprehension and future.. Energ. Environ. Sci.2012, 5,9936-9943.
 

Le Minoux D., Mahendra A., Lacroix-desmazes S., Limnios N., Friboulet A., Avalle B., Boquet D., Kaveri S. & Padiolleau-lefevre S. A novel molecular analysis of genes encoding catalytic antibodies.. Mol. Immunol.2012, 50,160-168.
 

Wootla B., Christophe O., Mahendra A., Dimitrov J., Repesse Y., Ollivier V., Friboulet A., Borel-derlon A., Levesque H., Borg J. Y., Andre S., Bayry J., Calvez T., Kaveri S. & Lacroix-desmazes S. Proteolytic antibodies activate factor IX in patients with acquired hemophilia. Blood2011, 117,2257-2264.
 

Gabibov A. G., Belogurov A. A., Lomakin Y. A., Zakharova M. Y., Avakyan M. E., Dubrovskaya V. V., Smirnov I. V., Ivanov A. S., Molnar A. A., Gurtsevitch V. E., Diduk S. V., Smirnova K. V., Avalle B., Sharanova S. N., Tramontano A., Friboulet A., Boyko A. N., Ponomarenko N. A. & Tikumova N. V. Combinatorial antibody library from Multiple Sclerosis patients reveals antibodies that cross-react with myelin basic protein and EBV-antigen.. FASEB J.2011, 25,4211-4221.
 

Zakharov A. V., Smirnov I. V., Serebryakova M. V., Dronina M. A., Kaznacheeva A. V., Kurkova I. N., Belogurov A. A., Friboulet A., Ponomarenko N. A., Gabibov A. G. & Bobik T. V. Expression of catalytic antibodies in eukaryotic systems. Mol. Biol.2011, 45,74-81.
 

Hegde P., Maddur M., Friboulet A., Bayry J. & Kaveri S. Viscum album exerts anti-inflammatory effect by selectively inhibiting cytokine-induced expression of cyclooxygenase-2.. PloS One2011, 6,e26312.
 

Stepanov A. V., Belogurov A. A., Ponomarenko N. A., Stremkovskly O. A., Kozlov L. V., Bichucher A. M., Dmitriev S. E., Shatsky I. N., Smirnov I. V., Shamborant O. G., Tonevitsky A. G., Friboulet A., Gabibov A. G. & Deyev S. M. Design of targeted B-cell killing agents.. PloS One2011, 6,e20991.
 

Smirnov I., Carletti E., Kurkova I., Nachon F., Nicolet Y., Mitkevich V., Debat H., Avalle B., Belogurov A., Kuznetsov N., Reshetnyak A., Masson P., Tonevitsky A., Ponomarenko N., Makarov A. A., Friboulet A., Tramontano A. & Gabibov A. G. Reactibodies generated by kinetic selection couple chemical reactivity with faborable protein dynamics.. Proc. Natl. Acad. Sci. USA2011, 108,15954-1959.
 

Phichith D., Bun S., Padiolleau-lefevre S., Guellier A., Banh S., Gallieni M., Frere J. M., Thomas D., Friboulet A. & Avalle B. Novel peptide inhibiting both TEM-1 beta-lactamase and penicillin-binding proteins.. FEBS J.2010, 277,4965-4972.
 

Elluru S. R., Duong Van Huyen J. P., Delignat S., Prost F., Heudes D., Kazatchkine M. D., Friboulet A. & Kaveri S. V. Antiangiogenic properties of viscum album extracts are associated with endothelial cytotoxicity.. Anticancer Res.2009, 29,2945-2950
  

Wootla B., Desirazu N. R., Friboulet A., Uda T., Lacroix-desmazes S. & Kaveri S. V. Varied immune response to FVIII: Presence of proteolytic antibodies directed to Factor VIII in different human pathologies.. Clin. Rev. Allerg. Immunol.2009, 37,97-104.
 

Kurkova I. N., Reshetnyak A. V., Durova O. M., Knorre V. D., Tramontano A., Friboulet A., Ponomarenko N. A., Gabibov A. G. & Smirnov I. V. Antibodes-antidotes against organophosphorus compounds?. Dokl. Biochem. Biophys.2009, 425,94-97.
 

Wootla B., Mahendra A., Dimitrov J. D., Friboulet A., Borel-derlon A., Rao D. N., Uda T., Borg J. Y., Bayry J., Kaveri S. V. & Lacroix-desmazes S. Factor VIII-hydrolyzing IgG in acquired and congenital hemophilia.. FEBS Letters2009, 583,2565-2572.
 

Durova O. M., Vorobiev I. I., Smirnov I. V., Reshetnyak A. V., Telegin G. B., Shamborant O. G., Orlova N. A., Genkin D. D., Bacon A., Ponomarenko N. A., Friboulet A. & Gabibov A. G. Strategies for induction of catalytic antibodies towards HIV-1 glycoprotein gp120 in autoimmune prone mice.. Mol. Immunol.2009, 47,87-95.
 

Phichith D., Bun S., Padiolleau-lefevre S., Banh S., Thomas D., Friboulet A. & Avalle B. Mutational and inhibitory analysis of a catalytic antibody. Implication for drug discovery.. Mol. Immunol.2009, 47,348-356.
 

Elluru S. R., Duong Van Huyen J. P., Delignat S., Kazatchkine M. D., Friboulet A., Kaveri S. V. & Bayry J. Induction of maturation and activation of human dendritic cells: A mechanism underlying the beneficial effect of Viscum album as complimentary therapy in cancer.. BMC Cancer2008, 8,161.
 

Smirnov I. V., Vorobiev I. I., Friboulet A., Avalle B., Thomas D., Knorre V. D., Gabibov A. G. & Ponomarenko N. A. The antiidiotypic approach to obtaining a proteolytic antibody.. Dokl. Biochem. Biophys.2008, 420,105-107.
 

Belogurov A. A., Kurkova I. N., Friboulet A., Thomas D., Misikov V. K., Zakharova M. U., Suchkov S. V., Kotov V., Alexin A. I., Avalle B., Souslova E. A., Morse H. C., Gabibov A. G. & Ponomarenko N. A. Recognition and Degradation of Myelin Basic Protein Peptides by Serum Autoantibodies as a Novel Biomarker for Multiple Sclerosis.. J. Immunol.2008, 180,1258-1267.
 

Wootla B., Dasgupta S., Dimitrov J. D., Bayry J., Levesque H., Borg J. Y., Borel-derlon A., Rao D. N., Friboulet A., Kaveri S. V. & Lacroix-desmazes S. FVIII hydrolysis mediated by anti-FVIII autoantibodies in acquired hemophilia.. J. Immunol.2008, 180,7714-7720.
 

Wootla B., Nicoletti A., Patey N., Dimitrov J. D., Legendre C., Christophe O. D., Friboulet A., Kaveri S. V., Lacroix-desmazes S. & Thaunat O. Hydrolysis of coagulation factors by circulating IgG is associated with a reduced risk for chronic allograft nephropathy in renal transplanted patients.. J. Immunol.2008, 180,8455-8460.
 

Ponomarenko N. A., Pillet D., Paon M., Vorobiev I. I., Smirnov I. V., Adenier H., Avalle B., Kolesnikov A. V., Kozyr A. V., Thomas D., Gabibov A. G. & Friboulet A. Anti-idiotypic antibody mimics proteolytic function of parent antigen.. Biochemistry 2007, 46,14598-14609.
 

Reshetnyak A. V., Armentano M. F., Morse H. C., Friboulet A., Makker S. P., Tramontano A., Knorre V. D., Gabibov A. G. & Ponomarenko N. A. Mechanism-dependent selection of immunoglobulin gene library for obtaining covalent biocatalysis.. Dokl. Biochem. Biophys.2007, 415,179-182.
 

Reshetnyak A. V., Armentano M. F., Ponomarenko N. A., Durova O., Ziganshin R., Serebryakova M., Govorun V., Gololobov G., Morse H. C., Friboulet A., Makket S. P. Gabibov A. G. & Tramontano A. Route to covalent catalysis by reactive selection for nascent protein nucleophile.. J. Am. Chem. Soc.2007, 129,16175-16182.
 

Ponomarenko N. A., Vorobiev I. I., Alexandrova E. S., Reshetnyak A. V., Telegin G. B., Khaidurov S. V., Avalle B., Karavanov A., Morse H. C., Thomas D., Friboulet A. & Gabibov A. G. Induction of protein-targeted catalytic response in autoimmune prone mice: antibody-mediated cleavage of HIV-1 glycoprotein gp120. . Biochemistry2006, 45,324-330.
 

Wootla B., Dasgupta S., Mallet V. O., Kazatchkine M. D., Nagaraja V., Friboulet A., Kaveri S. V. & Lacroix-desmazes S. Physiopathology of catalytic antibodies: the case for FVIII-hydrolyzing IgG. . Blood Coagul. Fibrin.2006, 17,229-234.
 

Padiolleau-lefevre S., Debat H., Phichith D., Thomas D., Friboulet A. & Avalle B. Expression of a functional scFv fragment of an anti-idiotypic antibody with a ß-lactam hydrolytic activity. . Immunol. Letters2006, 103,39-44.
 

Lacroix-desmazes S., Wootla B., Dasgupta S., Delignat S., Bayry J., Reinbold J., Hoebeke J., Saenko E., Kazatchkine M. D., Friboulet A., Christophe O., Nagaraja V. & Kaveri S. N. Catalytic IgG from patients with hemophilia A inactivate therapeutic factor VIII. . J. Immunol.2006, 177,1355-1363.
 

Ferreira J. S., Lozano R., Mondie S. & Friboulet A. Bifurcation analysis of a biochemical network. . Lecture Notes Contr. Inf.2006, 341,279-286.
 

Ponomarenko N. A., Durova O. M., Vorobiev I. I., Belogurov A. A., Telegin G. B., Suchkov S. V., Misikov V. K., Kiselev S. L., Lagarkova M. A., Govorun V. M., Serebryakova M. V., Avalle B., Tornatore P., Karavanov A., Morse H. C., Thomas D., Friboulet A. & Gabibov A. G. Autoantibodies to myelin basic protein catalyze site specific degradation of their antigen. . Proc. Natl. Acad. Sci. USA2006, 103,281-286.
 

BOOK

Saha C., Stephen-victor E., Friboulet A., Bayry J. & Kaveri S. V. Viscum album and its anti-inflammatory effect: angle of cytokine induced selective inhibition of COX-2.. Die Mistel in der Tumortherapie 42016, S. Scheer, A. Alban et al. eds.,pp. 197-203
  

Patent

B. Avalle-bihan, A. Friboulet, H. Isber, C. Loussouarn, S. Padiolleau-lefèvre. Inhibiteurs de STAT5 et utilisation de ceux-ci. 2014, ,.
 

A. Friboulet, B. Avalle-bihan, H. Débat, D. Thomas Composés capables de moduler l'activité et de stimuler la production d'un anticorps catalytique. France, n° 01067542001, ,.
 

Isabelle GOSSELIN

MC UPJV

      Contact Isabelle Gosselin

 

Articles

Mehmood N., Alayoubi R., Husson E., Jacquard C., Buchs J., Sarazin C. & Gosselin I. Kluyveromyces marxianus, an attractive yeast for ethanolic fermentation in the presence of imidazolium ionic liquids.. Int. J. Molec. Sci.2018, 19,887.
     ABSTRACT: Imidazolium ionic liquids (ILs) are promising solvents for lignocellulosic biomass (LCB) pretreatment and allow the achievement of higher ethanolic yields after enzymatic hydrolysis and ethanolic fermentation. However, residual ILs entrapped in pretreated biomass are often toxic for fermentative microorganisms, but interaction mechanisms between ILs and cells are still unknown. Here we studied the effects of 1-ethyl-3-methylimidazolium acetate [Emim][OAc] and 1-ethyl-3-methylimidazolium methylphosphonate [Emim][MeO(H)PO2] on Kluyveromyces marxianus, a thermotolerant ethanologenic yeast. Morphological impacts induced by ILs on K. marxianus were characterized by Scanning Electron Microscopy analysis and showed wrinkled, softened, and holed shapes. In Yeast-Malt-Dextrose (YMD) medium, K. marxianus tolerated IL additions up to 2% for [Emim][OAc] and 6% for [Emim][MeO(H)PO2]. Below these thresholds, some IL concentrations enhanced ethanolic yields up to +34% by switching the metabolic status from respiratory to fermentative. Finally, K. marxianus fermentation was applied on several substrates pretreated with [Emim][OAc] or [Emim][MeO(H)PO2] and enzymatically hydrolyzed: a model long fiber cellulose and two industrial LCBs, softwood (spruce) and hardwood (oak) sawdusts. The maximum ethanolic yields obtained were 1.8 to 3.9 times higher when substrates were pretreated with imidazolium ILs. Therefore K. marxianus is an interesting fermentative yeast in a second-generation bioethanol process implying IL pretreatment

Mehmood N., Husson E., Jacquard C., Wewetzer S., Buchs J., Sarazin C. & Gosselin I. Impact of two ionic liquids, 1-ethyl-3-methylimidazolium acetate and 1-ethyl-3-methylimidazolium methylphosphonate, on Saccharomyces cerevisiae: metabolic, physiologic, and morphological investigations.. Biotechnol. Biofuels 2015, 8,n.17.
 

Chaveriat L., Gosselin I., Machut C. & Martin P. Synthesis, surface tension properties and antibacterial activities of amphiphilic D-galactopyranose derivatives.. Eur. J. Med. Chem.2013, 62,177-186.
 

Husson E., Buchoux S., Avondo C., Cailleu D., Djellab K., Gosselin I., Wattraint O. & Sarazin C. Enzymatic hydrolysis of ionic liquid-pretreated celluloses: contribution of CP-MAS, 13C NMR and SEM.. Bioresource Technol.2011, 102,7335-7342.
 

Brique A., Devassine J., Pilard S., Cailleu D. & Gosselin I. Osmoregulated trehalose-derived oligosaccharides in Sinorhizobium meliloti.. FEBS Letters2010, 584,3661-3666.
 

Carlo GONZATO

MC UTC

           Contact Carlo Gonzato

 

 

Research

Member of the theme “Biomimicry and Biomolecular Diversity”.

Synthesis of molecularly imprinted nanomaterials and nanocomposites for biomimetic applications and bio/chemo-sensing. New polymerization techniques for imprinted materials. Synthesis of bio-mimicking functional polymers.

 

Skills

  • Polymer synthesis by radical techniques.
  • Polymer synthesis by bulk, dispersion, precipitation polymerization.
  • Polymer characterization: GPC, NMR, DLS, electrophoresis, rheology.
  • Polymerization-induced self-assembly.
  • Surface functionalization of nanomaterials.
  • Synthesis of composites nanomaterials.

 

General information

  • Current position: Lecturer
  • Research associate (UTC, FR)
  • Research associate (The University of Sheffield, UK)
  • PhD in biotechnology (UTC, FR)
  • R&D scientist (Montefibre SpA, IT)
  • Master degree in Chemistry (University of Padova, IT)

 

Personal interest

Hiking

 

Articles

Bonomi P., Attieh M. D., Gonzato C. & Haupt K. A novel versatile water soluble iniferter platform for the preparation of imprinted nanoparticles via photopolymerization in aqueous media.. Chem.-Eur. J.2016, 22,10150-10154.
 

Beyazit S., Tse Sum Bui B., Haupt K. & Gonzato C. Molecularly imprinted polymer nanomaterials and nanocomposites by controlled/living radical polymerization.. Prog. Polym. Sci.2016, 62,1-21.
 

Bompart M., Goto A., Wattraint O., Sarazin C., Tsujii Y., Gonzato C. & Haupt K. Molecularly imprinted polymers by reversible chain transfer catalyzed polymerization.. Polymer2015, 78,31-36.
 

Gonzato C., Pasetto P., Bedoui F., Mazeran P. E. & Haupt K. On the effect of using RAFT and FRP for the bulk synthesis of acrylic and methacrylic molecularly imprinted polymers.. Polymer Chem.2014, 5,1313-1322.
 

Gonzato C., Courty M., Pasetto P. & Haupt K. Magnetic molecularly imprinted polymer nanocomposites via surface-initiated RAFT polymerization.. Adv. Funct. Mater.2011, 21,3947-3953.
 

Eric HUSSON

MC UPJV

       Contact Eric Husson

 

Research

Member of the theme “Plant Metabolism and Bioresources”.

Room temperature ionic liquids and enzymes for the development of biorefinery strategies.
Skills

Enzymology in conventionnal and non-conventionnal media ; Ionic Liquids pretreatment of lignocellulosic, or chitinous biomasses; Separation / quantification techniques and structural characterization.
Roles and responsabilities

Involved in several reasearch projects (partner or leader), co-supervizer of PhD students.

Teaching in biochemistry, enzymology and microbiology at bachelor and master levels.
General information

  • Since 2011- Assistant Professor in Biochemistry – Enzyme and Cell Engineering UMR CNRS 7025, Université de Picardie Jules Verne, Amiens, France
  • 2011 – Post-doctoral position, Department of Food and Nutrition Sciences, Laval University, Québec, QC, Canada
  • 2010 – Post-doctoral position, Enzyme and Cell Engineering UMR CNRS 7025, Université de Picardie Jules Verne, Amiens, France
  • 2009 – Post-doctoral position (ATER), Laboratory of Reactions and Process Engineering UMR CNRS 7274, Université de Lorraine, Nancy, France
  • 2005-2008 – PhD, Laboratory of Reactions and Process Engineering UMR CNRS 7274, Université de Lorraine, Nancy, France

 

Personal interests

Scientific communication in priority education areas

 

Articles

Husson E., Auxenfans T., Herbaut M., Baralle M., Lambertyn V., Rakotoarivonina H., Remond C. & Sarazin C. Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases.. Bioresource Technol.2018, 251,280-287.
     ABSTRACT: Sequential and simultaneous strategies for fractioning wheat straw were developed in combining 1-ethyl-3-methyl imidazolium acetate [C2mim][OAc], endo-xylanases from Thermobacillus xylanilyticus and commercial cellulases. After [C2mim][OAc]-pretreatment, hydrolysis catalyzed by endo-xylanases of wheat straw led to efficient xylose production with very competitive yield (97.6?

Mehmood N., Alayoubi R., Husson E., Jacquard C., Buchs J., Sarazin C. & Gosselin I. Kluyveromyces marxianus, an attractive yeast for ethanolic fermentation in the presence of imidazolium ionic liquids.. Int. J. Molec. Sci.2018, 19,887.
     ABSTRACT: Imidazolium ionic liquids (ILs) are promising solvents for lignocellulosic biomass (LCB) pretreatment and allow the achievement of higher ethanolic yields after enzymatic hydrolysis and ethanolic fermentation. However, residual ILs entrapped in pretreated biomass are often toxic for fermentative microorganisms, but interaction mechanisms between ILs and cells are still unknown. Here we studied the effects of 1-ethyl-3-methylimidazolium acetate [Emim][OAc] and 1-ethyl-3-methylimidazolium methylphosphonate [Emim][MeO(H)PO2] on Kluyveromyces marxianus, a thermotolerant ethanologenic yeast. Morphological impacts induced by ILs on K. marxianus were characterized by Scanning Electron Microscopy analysis and showed wrinkled, softened, and holed shapes. In Yeast-Malt-Dextrose (YMD) medium, K. marxianus tolerated IL additions up to 2% for [Emim][OAc] and 6% for [Emim][MeO(H)PO2]. Below these thresholds, some IL concentrations enhanced ethanolic yields up to +34% by switching the metabolic status from respiratory to fermentative. Finally, K. marxianus fermentation was applied on several substrates pretreated with [Emim][OAc] or [Emim][MeO(H)PO2] and enzymatically hydrolyzed: a model long fiber cellulose and two industrial LCBs, softwood (spruce) and hardwood (oak) sawdusts. The maximum ethanolic yields obtained were 1.8 to 3.9 times higher when substrates were pretreated with imidazolium ILs. Therefore K. marxianus is an interesting fermentative yeast in a second-generation bioethanol process implying IL pretreatment

Brahim M., Checa Fernandez B. L., Regnier O., Bousseta N., Grimi N., Sarazin C., Husson E., Vorobiev E., Brosse N. Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straw’s lignin and pulps. Bioresource Technology2017, 237,42308
  

Auxenfans T., Husson E. & Sarazin C. Simultaneous pretreatment and enzymatic saccharification of (ligno)celluloses in aqueous-ionic liquid media: a compromise.. Biochem. Eng. J.2017, 117,77-86.
     ABSTRACT: In view of decreasing the amount of IL to achieve efficient simultaneous pretreatment and saccharification, a comprehensive study was undertaken. Different types of lignocellulosic biomasses were investigated in various enzymatic aqueous-IL systems including 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) or 1-ethyl-3-methylimidazolium methylphosphonate ([C2mim][MeO(H)PO2]). To better understand how 10% (v/v) of IL in the reaction medium led to the highest yields of glucose without fractionation from sawdust, distinct cellulosic models were then used as substrates. Kinetic studies demonstrated that both ILs affect synergistic action of cellulolytic enzymes depending on both constitutive anion and cellulosic substrate. Concentrations above 10% v/v of ILs deactivated cellulase even on highly digestible model substrates. ? and ? Kamlet-Taft parameters constituted more physicochemical pertinent indicators than apparent pH value to investigate effects of IL on cellulase performances. Fine description of these effects was proposed onto individual EG, CBH and BG. [C2mim][MeO(H)PO2] was demonstrated a better compatible IL for enzymes up to a concentration of 30% (v/v). The efficiency of simultaneous pretreatment and saccharification was governed by a compromise between better substrate accessibility and enzyme deactivation.

Mehmood N., Husson E., Jacquard C., Wewetzer S., Buchs J., Sarazin C. & Gosselin I. Impact of two ionic liquids, 1-ethyl-3-methylimidazolium acetate and 1-ethyl-3-methylimidazolium methylphosphonate, on Saccharomyces cerevisiae: metabolic, physiologic, and morphological investigations.. Biotechnol. Biofuels 2015, 8,n.17.
 

Hulin L., Husson E., Bonnet J. P., Stevanovic T. & Sarazin C. Enzymatic transesterification of kraft lignin with long acyl chains in ionic liquids.. Molecules2015, 20,16334-16353.
 

Auxenfans T., Buchoux S., Husson E. & Sarazin C. Efficient enzymatic saccharification of Miscanthus: energy-saving by combinig dilute acid and ionic liquid pretreatments.. Biomass Bioenerg.2014, 62,82-92.
 

Auxenfans T., Buchoux S., Larcher T., Husson G., Husson E. & Sarazin C. Enzymatic saccharification and structural properties of industrial wood sawdust: recycled ionic liquids pretreatments.. Energ. Convers. Manage.2014, 88,1094-1103.
 

Doyen A., Husson E. & Bazinet L. Use of an electrodialytic reactor for the simultaneous ß-lactoglobulin enzymatic hydrolysis and fractionation of generated bioactive peptides.. Food Chem.2013, 136,1193-1202.
 

Husson E., Araya-farias M., Dejardins Y. & Bazinet L. Selective anthocyanins enrichment of cranberry juice by electrophoresis with multiple ultrafiltration membranes stacked.. Innov. Food Sci. Emerg. Technol. 2013, 17,153-162.
 

Husson E., Araya-farias M., Gagne A. & Bazinet L. Selective anthocyanins enrichment of cranberry juice by electrodialysis with filtration membranes: influence of membrane characteristics.. J. Membrane Sci.2013, 448,114-124.
 

Auxenfans T., Buchoux S., Djellab K., Avondo C., Husson E. & Sarazin C. Mild pretreatment and enzymatic saccharification of cellulose with recycled ionic liquid towards one-batch process.. Carbohydr. Polym.2012, 90,805-813.
 

Husson E., Buchoux S., Avondo C., Cailleu D., Djellab K., Gosselin I., Wattraint O. & Sarazin C. Enzymatic hydrolysis of ionic liquid-pretreated celluloses: contribution of CP-MAS, 13C NMR and SEM.. Bioresource Technol.2011, 102,7335-7342.
 

BOOK

Chavelot I, Arab-tehrany E, Husson E, Gerardun C, Vandamme E, Luis Revuelta J. Application of carnosine and its functionalised derivatives. Industrial Biotechnology of vitamins, biopigments, and antioxidants 2016, E.J. Vandamme & J.L. Revuelta eds. ,pp. 421-444
  

Patent

E. Husson, C. Sarazin, H. Ducatel, I. Murrieta-pazos, G. Ravot Procédé de traitement de la biomasse. 2017, ,.
 

Karsten HAUPT

PR UTC - Director

        Contact Karsten Haupt

Articles

Medina Rangel P., Caclef S., Xu J., Panagiotopoulou M., Kovensky J., Tse Sum Bui B., Haupt K. Solid-phase synthesis of moleculary imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans. Scientific Reports2019, 9,3923.
 

Xu J., Merlier F., Avalle B., Veillard V., Debré P., Haupt K., Tse Sum Bui B. Molecularly Imprinted Polymer Nanoparticles as Potential Synthetic Antibodies for Immunoprotection against HIV . ACS Appl. Mater. Interfaces2019, 11,9824-9831.
     ABSTRACT: We describe the preparation and characterization of synthetic antibodies based on molecularly imprinted polymer nanoparticles (MIP-NPs) for the recognition and binding of the highly conserved and specific peptide motif SWSNKS (3S), an epitope of the envelope glycoprotein 41 (gp41) of human immunodeficiency virus type 1 (HIV-1). This motif is implicated in the decline of CD4+ T cells and leads to the deterioration of the immune system during HIV infection. Therefore, the development of MIP-NPs that can target and block the 3S peptide to prevent subsequent cascade interactions directed toward the killing of CD4+ T cells is of prime importance. Because most antibodies recognize their protein antigen via a conformational or structured epitope (as opposed to a linear epitope commonly used for molecular imprinting), we employed protein molecular modeling to design our template epitope so that it mimics the three-dimensional structure fold of 3S in gp41. The resulting template peptide corresponds to a cyclic structure composed of CGSWSNKSC, with the 3S motif well orientated for imprinting. MIP-NPs with a size of 65 nm were obtained by solid-phase synthesis and were water-soluble. They were prepared by a judicious combination of multiple functional monomers affording hydrogen bonding, ionic, π–π, and hydrophobic interactions, conferring high affinity and selectivity toward both the cyclic peptide and the whole gp41 protein. These results suggest that our MIPs could potentially be used for blocking the function of the 3S motif on the virus.

Xu J., Prost E., Haupt K. & Tse Sum Bui B. Direct and sensitive determination of trypsin in human urine using a water-soluble signaling fluorescent molecularly imprinted polymer nanoprobe. . Sensor. Actuat. B-Chem. 2018, 258,10-17.
     ABSTRACT: https://doi.org/10.1016/j.snb.2017.11.077

Xu J, Haupt K. & Tse Sum Bui B. Core-shell molecularly imprinted polymer nanoparticles as synthetic antibodies in a sandwich fluoroimmunoassay for trypsin determination in human serum.. ACS Appl. Mater. Interfaces2017, 9, 24476?24483.
     ABSTRACT: We describe the application of a fluorescently labeled water-soluble core

Panagiotopoulou M., Kunath S., Medina-rangel P. X., Haupt K. & Tse Sum Bui B. Fluorescent molecularly imprinted polymers as plastic antibodies for selective labeling and imaging of hyaluronan and sialic acid on fixed and living cells.. Biosens. Bioelectron.2017, 88,85-93.
     ABSTRACT: Altered glycosylation levels or distribution of sialic acids (SA) or hyaluronan in animal cells are indicators of pathological conditions like infection or malignancy. We applied fluorescently-labeled molecularly imprinted polymer (MIP) particles for bioimaging of fixed and living human keratinocytes, to localize hyaluronan and sialylation sites. MIPs were prepared with the templates D-glucuronic acid (GlcA), a substructure of hyaluronan, and N-acetylneuraminic acid (NANA), the most common member of SA. Both MIPs were found to be highly selective towards their target monosaccharides, as no cross-reactivity was observed with other sugars like N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, D-glucose and D-galactose, present on the cell surface. The dye rhodamine and two InP/ZnS quantum dots (QDs) emitting in the green and in the red regions were used as fluorescent probes. Rhodamine-MIPGlcA and rhodamine-MIPNANA were synthesized as monodispersed 400 nm sized particles and were found to bind selectively their targets located in the extracellular region, as imaged by epifluorescence and confocal microscopy. In contrast, when MIP-GlcA and MIP-NANA particles with a smaller size (125 nm) were used, the MIPs being synthesized as thin shells around green and red emitting QDs respectively, it was possible to stain the intracellular and pericellular regions as well. In addition, simultaneous dual-color imaging with the two different colored QDs-MIPs was demonstrated. Importantly, the MIPs were not cytotoxic and did not affect cell viability; neither was the cells morphology affected as demonstrated by live cell imaging. These synthetic receptors could offer a new and promising imaging tool to monitor disease progression.

Nestora S., Merlier F., Prost E., Haupt K., Rossi C. & Tse Sum Bui B. Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer.. J. Chromatogr. A2016, 1465,47-54.
 

Canfarotta F., Waters A., Sadler R., Mcgill P., Guerreiro A., Papkovsky D., Haupt K. & Piletsky S. Biocompatibility and internalization of moleculary imprinted nanoparticles.. Nano Res.2016, 9,3463-3477.
 

Chia Gomez L. P., Spangenberg A., Ton X. A., Fuchs Y., Bokeloh F., Malval J. P., Tse Sum Bui B., Thuau D., Ayela C., Haupt K. & Soppera O. Rapid prototyping of chemical microsensors based on molecularly imprinted polymers synthesized by two-photon stereolithography.. Adv. Mater.2016, 28,5931-5937.
 

Parlak O., Beyazit S., Jafari M. J., Tse Sum Bui B., Haupt K., Tiwari A. & Turner A. P. F. Light-triggered switchable graphene-polymer hybrid bioelectronics.. Adv. Mater. Interfaces2016, 3,1500353.
 

Panagiotopoulou M., Salinas Y., Beyazit S., Kunath S., Mayes A. G., Duma L., Prost E., Resmini M., Tse Sum Bui B. & Haupt K. Molecularly imprinted polymer-coated quantum dots for multiplexed cell targeting and imaging.. Angew. Chem. Int. Ed.2016, 55,8244-8248.
 

Nestora S., Merlier F., Beyazit S., Prost E., Duma L., Baril B., Graves A., Haupt K. & Tse Sum Bui B. Plastic antibodies for cosmetics: Molecularly imprinted polymers scavenge precursors of malodors. . Angew. Chem. Int. Ed.2016, 55,6252-6256.
 

Xu J. J., Ambrosini S., Tamahkar E., Rossi C., Haupt K. & Tse Sum Bui B. Toward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins.. Biomacromolecules2016, 17,345-353.
 

Bonomi P., Attieh M. D., Gonzato C. & Haupt K. A novel versatile water soluble iniferter platform for the preparation of imprinted nanoparticles via photopolymerization in aqueous media.. Chem.-Eur. J.2016, 22,10150-10154.
 

Parlak O., Beyazit S., Tse Sum Bui B., Haupt K., Turner A. P. F. & Tiwari A. Programmable bioelectronics in a stimuli-encoded 3D graphene interface.. Nanoscale2016, 8,9976-9981.
 

Beyazit S., Tse Sum Bui B., Haupt K. & Gonzato C. Molecularly imprinted polymer nanomaterials and nanocomposites by controlled/living radical polymerization.. Prog. Polym. Sci.2016, 62,1-21.
 

Kunath S., Panagiotopoulou M., Maximilien J., Marchyk N., Sanger J. & Haupt K. Cell and tissue imaging with molecularly imprinted polymers as plastic antibody mimics.. Adv. Healthc. Mater.2015, 4,1322-1326.
 

Adali-kaya Z., Tse Sum Bui B., Falcimaigne-cordin A. & Haupt K. Molecularly imprinted polymer nanomaterials and nanocomposites: atom-transfer radical polymerization with acidic monomers.. Angew. Chem. Int. Ed.2015, 54,192-195.
 

Starok M., Preira P., Vayssade M., Haupt K., Salome L. & Rossi C. EGFR inhibition by curcumin in cancer cells: a dual mode of action.. Biomacromolecules2015, 16,1634-1642.
 

Ton X. A., Acha V., Bonomi P., Tse Sum Bui B. & Haupt K. A disposable evanescent wave fiber optic sensor coated with a molecularly imprinted polymer as a selective fluorescent probe.. Biosens. Bioelectron.2015, 64,359-366.
 

Panagiotopoulou M., Beyazit S., Nestora S., Haupt K., & Tse Sum Bui B. Initiator-free synthesis of molecularly imprinted polymers by polymerization of self-initiated monomers.. Polymer2015, 66,43-51.
 

Bompart M., Goto A., Wattraint O., Sarazin C., Tsujii Y., Gonzato C. & Haupt K. Molecularly imprinted polymers by reversible chain transfer catalyzed polymerization.. Polymer2015, 78,31-36.
 

Foguel M. V., Ton X. A., Zanoni M. V. B., Sotomayor M. D. P. T., Haupt K. & Tse Sum Bui B. A molecularly imprinted polymer-based evanescent wave fiber optic sensor for the detection of basic red 9 dye. . Sens. Actuators B2015, 218,222-228.
 

Fuchs Y., Kunath S., Soppera O., Haupt K. & Mayes A. G. Molecularly imprinted silver-halide reflection holograms for label-free opto-chemical sensing.. Adv. Funct. Mater.2014, 24,688-694.
 

Ayela C., Dubourg G., Pellet C. & Haupt K. All-organic micromechanical systems integrating specific molecular recognition. A new generation of chemical sensors.. Adv. Mater.2014, 26,5876-5879.
 

Beyazit S., Ambrosini S., Marchyk N., Palo E., Kale V., Soukka T., Tse Sum Bui B. & Haupt K. Versatile synthetic strategy for coating upconverting nanoparticles with polymer shells through localized photopolymerization by using the particles as internal light sources.. Angew. Chem. Int. Ed.2014, 53,8919-8923.
 

Li B., Xu J., Hall A. J., Haupt K. & Tse Sum Bui B. Water-compatible silica sol-gel molecularly imprinted polymer as potential delivery system for the controlled release of salicylic acid.. J. Mol. Recognit.2014, 27,559-565.
 

Marchyk N., Maximilien J., Beyazit S., Haupt K. & Tse Sum Bui B. One-pot synthesis of iniferter-bound polystyrene core nanoparticles for the controlled grafting of multilayer shells.. Nanoscale2014, 6,2872-2878.
 

Gonzato C., Pasetto P., Bedoui F., Mazeran P. E. & Haupt K. On the effect of using RAFT and FRP for the bulk synthesis of acrylic and methacrylic molecularly imprinted polymers.. Polymer Chem.2014, 5,1313-1322.
 

Cakir P., Cutivet A., Resmini M., Tse Sum Bui B. & Haupt K. Protein-size molecularly imprinted polymer nanogels as synthetic antibodies, by localized polymerization with multi-initiators.. Adv. Mater.2013, 25,1048-1051.
 

Fuchs Y., Soppera O., Mayes A. G. & Haupt K. Holographic molecularly imprinted polymers for label-free chemical sensing.. Adv. Mater.2013, 25,566-570.
 

Ton X. A., Tse Sum Bui B., Resmini M., Bonomi P., Dika I., Soppera O. & Haupt K. A versatile fiber-optic fluorescence sensor based on molecularly imprinted microstructures polymerized in situ.. Angew. Chem. Int. Ed.2013, 52,8317-8321.
 

Ambrosini S., Beyazit S., Haupt K. & Tse Sum Bui B. Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition.. Chem. Commun.2013, 49,6746-6748.
 

Zdunek J., Benito-pena E., Linares A., Falcimaigne-cordin A., Orellana G., Haupt K. & Moreno-bondi M. C. Surface-imprinted nanofilaments for Europium-amplified luminescent detection of fluoroquinolones antibiotics.. Chem.-Eur. J.2013, 19,10209-10216.
 

Dmitrienko E. V., Bulushev R. D., Haupt K., Kosolobov S. S., Latyshev A. V., Pyshnaya I. A. & Pyshnyi D. V. A simple approach to prepare molecularly imprinted polymers from nylon-6.. J. Mol. Recognit.2013, 26,368-375.
 

Kantarovich K., Tsarfati-barad I., Gheber L. A., Haupt K. & Bar I. Reading biochips by raman and surface-enhanced raman spectroscopies.. Plasmonics2013, 8,3-12.
 

Kunath S., Marchyk N., Haupt K. & Feller K. H. Multi-objective optimization and design of experiments as tools to tailor molecularly imprinted polymers specific for glucuronic acid.. Talanta2013, 105,211-218.
 

Fuchs Y., Soppera O. & Haupt K. Photopolymerization and photostructuring of molecularly imprinted polymers for sensor applications-A review.. Anal. Chim. Acta2012, 717,7-20.
 

Ton X. A., Acha V., Haupt K. & Tse Sum Bui B. Direct fluorimetric sensing of UV-excited analytes in biological and environmental samples using molecularly imprinted polymer nanoparticles and fluorescence polarization.. Biosens. Bioelectron.2012, 36,22-28.
 

Doumiati S., Haupt K. & Rossi C. Autophosphorylation activation and inhibition by curcumin of the epidermal growth factor receptor reconstituted in liposomes.. J. Mol. Recognit.2012, 25,623-629.
 

Bompart M., Haupt K. & Ayela C. Micro and nanofabrication of molecularly imprinted polymers.. Top. Curr. Chem.2012, 325,83-110.
 

Haupt K., Linares A. V., Bompart M. & Tse Sum Bui B. Molecularly imprinted polymers.. Top. Curr. Chem.2012, 325,1-28.
 

Gonzato C., Courty M., Pasetto P. & Haupt K. Magnetic molecularly imprinted polymer nanocomposites via surface-initiated RAFT polymerization.. Adv. Funct. Mater.2011, 21,3947-3953.
 

Fuchs Y., Linares A. V., Mayes A. G., Haupt K. & Soppera O. Ultrathin selective molecularly imprinted polymer microdots obtained by evanescent wave photopolymerization.. Chem. Mater.2011, 23,3645-3651.
 

Servant A., Haupt K. & Resmini M. Tuning molecular recognition in water-soluble nanogels with enzyme-like activity for Kemp elimination.. Chem.-Eur. J.2011, 17,11052-11059.
 

Harz S., Shimmelpfennig M., Tse Sum Bui B., Marchyk N., Haupt K. & Feller K. H. Fluorescence optical spectrally resolved sensor based on molecularly imprinted polymers and microfluidics.. Eng. Life Sci.2011, 11,559-565.
 

Tse Sum Bui B. & Haupt K. Preparation and evaluation of a molecularly imprinted polymer for the selective recognition of testosterone – application to molecularly imprinted sorbent assays.. J. Mol. Recognit.2011, 24,1123-1129.
 

Diot J. D., Moreno I. G., Twigg G., Ortiz Mellet C., Haupt K., Butters T. D., Kovensky J. & Gouin S. G. Amphiphilic 1-deoxynojirimycin derivatives through click strategies for chemical chaperoning in N370S Gaucher cells.. J. Org. Chem.2011, 76,7757-7768.
 

Piperno S., Tse Sum Bui B., Haupt K. & Ghebert L. A. Immobilization of molecularly imprinted polymer nanoparticles in electrospun poly(vinyl alcohol) nanofibers.. Langmuir2011, 27,1547-1550.
 

Linares A. V., Falcimaigne-cordin A., Gheber L. A. & Haupt K. Patterning nanostructured, synthetic, polymeric receptors by simultaneous projection photolithography, nanomolding, and molecular imprinting.. Small2011, 7,2318-2325.
 

Bompart M., De Wilde Y. & Haupt K. Chemical nanosensors based on composite molecularly imprinted polymer particles and surface-enhanced Raman scattering.. Adv. Mater.2010, 22,2343-2348.
 

Tse Sum Bui B. & Haupt K. Molecularly imprinted polymers : synthetic receptors in bioanalysis. Anal. Bioanal. Chem.2010, 398,2481-2492.
 

Tse Sum Bui B., Merlier F. & Haupt K. Towards the use of a molecularly imprinted polymer in doping analysis :selective preconcentration and analysis of testosterone and epitestosterone in human urine.. Anal. Chem.2010, 82,4420-4427.
 

Kantarovich K., Tsarfati I., Gheber L. A., Haupt K. & Bar I. Reading microdots of a molecularly imprinted polymer by surface-enhanced Raman spectroscopy.. Biosens. Bioelectron.2010, 26,809-814.
 

Lalo H., Ayela C., Dague E., Vieu C. & Haupt K. Nanopatterning molecularly imprinted polymers by soft lithography: a hierarchical approach.. Lab Chip2010, 10,1316-1318.
 

Haupt K. Plastic antibodies.. Nature Mater.2010, 9,612-614.
 

Claude B., Viron-lamy C., Haupt K. & Morin P. Synthesis of a molecularly imprinted polymer for the solid-phase extraction of betulin and betulinic acid from plane bark.. Phytochem. Anal.2010, 21,180-185.
 

Linares A. V., Vandevelde F., Pantigny J., Falcimaigne-cordin A. & Haupt K. Polymer films composed of surface-bound nanofilaments with a high aspect ratio, molecularly imprinted with small molecules and proteins.. Adv. Funct. Mater.2009, 19,1299-1303.
 

Kantarovich K., Tsarfati I., Gheber L. A., Haupt K. & Bar I. Writing droplets of molecularly imprinted polymers by nano fountain pen and detecting their molecular interactions by surface-enhanced Raman scattering.. Anal. Chem.2009, 81,5686-5690.
 

Kantarovich K., Belmont A. S., Haupt K., Bar I. & Gheber L. A. Detection of template binding to molecularly imprinted polymers by Raman spectroscopy.. Appl. Phys. Lett.2009, 94,194103.
 

Bompart M. & Haupt K. Molecularly imprinted polymers and controlled/living radical polymerization.. Aust. J. Chem.2009, 62,751-761.
 

Bompart M., Geher L. A., De Wilde Y. & Haupt K. Direct detection of analyte binding to single molecularly imprinted polymer particles by confocal Raman spectroscopy.. Biosens. Bioelectron.2009, 25,568-571.
 

El Kirat K., Bartkowski M. & Haupt K. Probing the recognition specificity of a protein molecularly imprinted polymer using force spectroscopy.. Biosens. Bioelectron.2009, 24,2618-2624.
 

Cutivet A., Schembri C., Kovensky J. & Haupt K. Molecularly imprinted microgels as enzyme inhibitors.. J. Am. Chem. Soc.2009, 131,14699-14702.
 

Mhaka B., Cukrowska E., Tse Sum Bui B., Ramstrom O., Haupt K., Tutu H. & Chimuka L. Selective extraction of triazine herbicide from food samples based on a combination of a liquid membrane and molecularly imprinted polymers.. J. Chromatogr. A2009, 1216,6796-6801.
 

Guillon S., Lemaire R., Linares A. V., Haupt K. & Ayela C. Single step patterning of molecularly imprinted polymers for large scale fabrication of microchips.. Lab Chip2009, 9,2987-2991.
 

Diot J., Garcia-moreno M. I., Gouin S. G., Mellet C. O., Haupt K. & Kovensky J. Multivalent iminosugars to modulate affinity and selectivity for glycosidases.. Org. Biomol. Chem.2009, 7,357-363.
 

Tse Sum Bui B., Belmont A. S., Witters H. & Haupt K. Molecular recognition of endocrine disruptors by synthetic and natural 17beta-estradiol receptors: a comparative study.. Anal. Bioanal. Chem.2008, 390,2081-2088.
 

Vandevelde F., Pantigny J., Pezron I., Gheber L. & Haupt K. Nanostructured molecularly imprinted polymer films as synthetic recognition layers.. Int. J. Nanotechnology2008, 5,757-768.
 

Claude B., Morin P., Lafosse M., Belmont A. S. & Haupt K. Selective solid-phase extraction of a triterpene acid from a plant extract by molecularly imprinted polymer.. Talanta2008, 75,344-350.
 

Vandevelde F., Belmont A. S., Pantigny J. & Haupt K. Hierarchically nanostructured polymer films based on molecularly imprinted surface-bound nanofilaments.. Adv. Mater.2007, 19,3717-3720.
 

Rossi C. & Haupt K Application of the Doehlert experimental design to molecularly imprinted polymers: surface response optimization of specific template recognition as a function of the type and degree of cross-linking.. Anal. Bioanal. Chem.2007, 389,455-460.
 

Ayela C., Vandevelde F., Lagranfe D., Haupt K. & Nicu L. Combining resonant piezoelectric micromembranes with molecularly imprinted polymers.. Angew. Chem. Int. Ed.2007, 46,9271-9274.
 

Belmont A. S., Sokuler M., Haupt K. & Gheber L. A. Direct writing of molecularly imprinted microstructures using a nanofountain pen.. Appl. Phys. Lett.2007, 90,193101.
 

Belmont A. S., Jaeger S., Knopp D., Niessner R., Gauglitz G. & Haupt K. Molecularly imprinted polymer films for reflectometric interference spectroscopic sensors.. Biosens. Bioelectron.2007, 22,3267-3272.
 

Vandevelde F., Leichle T., Ayela C., Bergaud C., Nicu L. & Haupt K. Direct patterning of molecularly imprinted microdot arrays for sensors and biochips.. Langmuir2007, 23,6490-6493.
 

Sineriz F., Ikeda Y., Petit E., Bultel L., Haupt K., Kovensky J. & Papy-garcia D. Toward an alternative for specific recognition of sulfated sugars. Preparation of highly specific molecular imprinted polymers.. Tetrahedron2007, 63,1857-1862.
 

Grafe A., Haupt K. & Mohr G. J. Optical sensor materials for the detection of amines in organic solvent. . Anal. Chim. Acta2006, 565,42-47.
 

Hunt C. E., Pasetto P., Ansell R. J. & Haupt K. A fluorescence polarisation molecular imprint sorbent assay for 2,4-D : a non separation pseudo immunoassay. . Chem. Commun.2006, 16,1754-1756.
 

Pichon V. & Haupt K. Affinity separations on molecularly imprinted polymers with special emphasis on solid-phase extraction. . J. Liquid Chromatogr.2006, 29,989-1023.
 

BOOK

Bokeloh F., Ayela C. & Haupt K. Micro and nanofabrication of molecularly imprinted polymers . Molecularly Imprinted Polymers for Analytical Chemistry Applications 2018, W. Kutner and P. Sindhu Sharma,pp. 167-197
  

Xu J, Medina-rangel P. X., Haupt K. & Tse Sum Bui B. Guide to the preparation of molecularly imprinted polymer nanoparticles for protein recognition, by solid-phase synthesis. Methods Enzymol2017, ,pp.115-141
  

Panagiotopoulou M., Kunath S., Haupt K., Tse Sum Bui B. Cell and Tissue Imaging with Molecularly Imprinted Polymers . Methods in Molecular Biology2017, ,PP.399-415
  

Maximilien J., Beyazit S., Rossi C., Haupt K. & Tse Sum Bui B Nanoparticles in biomedical applications. Measuring Biological impacts of nanomaterials2016, Springer International Publishing, Switzerland,pp. 177-210
  

Irene MAFFUCCI

MC UTC

        Contact Irene Maffucci

Research

Within the theme “Biomimicry and Biomolecular Diversity”, she is involved in the rational design of potentially bioactive molecules, such as molecular imprinted polymers (MIPs) targeting macrobiomolecules, aptamers and peptidomimetics. This implies the application of multiple molecular modeling techniques, from docking, to enhanced sampling molecular dynamics, to binding affinity predictions.

In addition, she is also interested in the design and optimisation of protein-protein interaction modulators within virtual screening procedures.

 

Skills

During her research career, she developed different molecular modeling competences, such as docking, peptide secondary structure prediction, binding affinity prediction, enzymatic activity investigation and protein thermostability studies. This implied the mastering of docking, molecular dynamics, ehnanced molecular dynamics and mixed QM/MM techniques.

 

Roles and responsabilities

She is responsible of setting up the bioinformatic core within the GEC. She also takes part in the teaching activities at UTC, being in charge of the Unité d’Enseignement focused on the methods and tools for bioinformatics.

 

General information

After a master in Chemistry and Pharmaceutical Technologies in 2012 at the University of Milan, in 2015 she got a PhD in Drug Chemistry at the University of Milan under the supervision of Prof. Alessandro Contini and working on the development and application of computational methods for the design of protein-protein interactions modulations. Within her PhD, in 2014, she collaborated with the organic chemists of the Prof. Clayden’s group at the University of Manchester on the rational conformational control of helical peptides. From 2016 to 2018, under the supervision of Dr. Damien Laage, Fabio Sterpone and Guillaume Stirnemann, she held a post-doctoral position within a collaboration between the theoretical pole of the Ecole Normale Supérieure and the Laboratorie de Biochimie Théorique at the Institut de Biologie Physico-Chimique in Paris. Her project aimed to understand the effect of multiple mutations on the structural stability and activity of the dihydrofolate reductase. She joined the group GEC in September 2018.

 

Personal interests 

She likes reading, cinema and theatre. She loves travelling and cooking.

 

Articles

Yolande PERRIN

PR UTC

         Contact Yolande Perrin

 

Research

I am studying the enzymatic pathways of intracellular fatty acid trafficking in the context of lipid metabolism of oilseed plants (rape, flax, castor oil, Arabidopsis). On the one hand, my interests go on the implication of carnitine in the plant physiology (Charrier et al., 2012, Jacques et al., 2018), in particular on the intracellular transport of fatty acids during lipids syntheses (Bourdin et al., 2007, Nguyen et al., 2016). On the other hand, I study the enzymatic processes of fatty acid transfer during the synthesis of glycerolipids membrane or reserve, in the context of atypical fatty acid management in particular (Fahs et al., 2019). My research contributes to the knowledge of lipid synthesis modalities in oleaginous plants, and to the identification of transferable functions to biotechnological systems for the production of high added value lipids.

 

Skills
Plant biotechnology, Plant physiology, Lipid metabolism, Molecular biology, Biochemistry

 

Roles and responsabilities
In charge of Engineering Training in Biological Engineering at UTC

  • General information
  • 1986: DEUG, Science of nature and life
  • 1987: Bachelor’s degree in Biology of organisms, biology option
  • 1988: Master’s degree in Biology of organisms and populations, plant physiology option
  • 1989: DEA in Agricultural sciences, Physiology applied to plant productions
  • 1990-1994: PhD in Plant physiology, University of Montpellier 2
  • 1994: Postdoctoral researcher at the University of Picardie Jules Vernes
  • 1995-2001: Postdoctoral researcher at John Innes, Virology department, Molecular Biotechnology, Biology Chemistry department
  • 2001: Assistant Professor at UTC (section CNU 64), UMR CNRS 7025, GEC
  • 2012: HDR, UTC
  • 2016: Professor at UTC, UMR CNRS 7025, GEC

Articles

Fahs Z., Rossez Y., Guénin S., Gutierrez L., Thomasset B., & Perrin, Y. Cloning and molecular characterization of three lysophosphatidic acid acyltransferases expressed in flax seeds. Plant Science2019, 280,41-50.
 

Jacques F., Rippa S., Perrin Y. Physiology of L-carnitine in plants in light of the knowledge in animals and microorganisms. Plant Science2018, 9,1170.
     ABSTRACT: L-carnitine is present in all living kingdoms where it acts in diverse physiological processes. It is involved in lipid metabolism in animals and yeasts, notably as an essential cofactor of fatty acid intracellular trafficking. Its physiological significance is poorly understood in plants, but L-carnitine may be linked to fatty acid metabolism among other roles. Indeed, carnitine transferases activities and acylcarnitines are measured in plant tissues. Current knowledge of fatty acid trafficking in plants rules out acylcarnitines as intermediates of the peroxisomal and mitochondrial fatty acid metabolism, unlike in animals and yeasts. Instead, acylcarnitines could be involved in plastidial exportation of de novo fatty acid, or importation of fatty acids into the ER, for synthesis of specific glycerolipids. L-carnitine also contributes to cellular maintenance though antioxidant and osmolyte properties in animals and microbes. Recent data indicate similar features in plants, together with modulation of signaling pathways. The biosynthesis of L-carnitine in the plant cell shares similar precursors as in the animal and yeast cells. The elucidation of the biosynthesis pathway of L-carnitine, and the identification of the enzymes involved, is today essential to progress further in the comprehension of its biological significance in plants.

Nguyen P. J., Rippa S., Rossez Y. & Perriny. Acylcarnitines participate in developmental processes associated to lipid metabolism in plants.. Planta2016, 243,1011-1022.
 

Rippa S., Zhao Y., Merlier F., Charrier A. & Perrin Y. The carnitine biosynthetic pathway in Arabidopsis thaliana shares similar features with the pathway of mamals and fungi.. Plant Physiol. Biochem.2012, 60,109-114.
 

Charrier A., Rippa S., Yu A., Nguyen P. J., Renou J. P. & Perrin Y. The effect of carnitine on Arabidopsis development and recovery in salt stress conditions.. Planta2012, 235,123-135.
 

Picard N., Guenin S., Perrin Y., Hilaire G. & Larnicol N. Consequences of prenatal exposure to diazepam on the respiratory parameters, respiratory network activity and gene expression of a1 and alpha-2 subunits of GABA(A) receptor in newborn rat.. Adv. Exp. Biol. Med.2008, 605,144-148.
 

Picard N., Guenin S., Perrin Y. & Larnicol N. Maternal caffeine ingestion during gestation and lactation influences respiratory adaptation to acute alveolar hypoxia in newborn rats and adenosine A2A and GABA A receptor mRNA transcription.. Neuroscience2008, 156,630-639.
 

Picard N., Guenin S., Perrin Y., Hilaire G. & Larnicol N. Prenatal diazepam exposure alters respiratory control system and Gabaa and adenosine receptor gene expression in newborn rats.. Pediatr. Res.2008, 64,44-49.
 

Lelandais-briere C., Jovanovic M., Torres G. A., Perrin Y., Lemoine R., Corre-menguy F. & Hartmann C. Disruption of AtOCT1, an organic cation transporter gene, affects root development and carnitine-related responses in Arabidopsis.. Plant J.2007, 51,154-164.
 

Bourdin B., Adenier H. & Perrin Y. Carnitine is associated with fatty acid metabolism in plants.. Plant Physiol. Biochem.2007, 45,926-931.
 

Alamillo J. M., Monger W., Sola I., Garcia B., Perrin Y., Bestagno M., Burrone O. R., Plana-duran J., Enjuanes L., Lomonossoff G. P. & Garcia J. A. Use of virus vectors for the expression in plants of active full-length and single-chain anti-coronavirus antibodies. . Biotechnol. J.2006, 1,1103-1111.
 

Canizares M. C., Liu L., Perrin Y., Tsakiris E. & Lomonossoff G. P. A bipartite system for the constitutive and inducible expression in high levels of foreign proteins in plants. . Plant Biotechnol. J.2006, 4,183-193.
 

Monger W., Alamillo J. M., Sola I., Perrin Y., Bestagno M., Burrone O. R., Sabella P., Plana-duran J., Enjuanes L., Garcia J. A. & Lomonossoff G. P. An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus infection when supplied orally in crude plant extracts. . Plant Biotechnol. J.2006, 4,623-631.
 

Séverine PADIOLLEAU

MC UTC

       Contact Séverine Padiolleau

 

Research

Member of the theme “Biomimicry and Biomolecular Diversity”.

Genesis and exploitation of the molecular diversity for fundamental and applied purposes. Research activity focuses on the diversity of the immune repertoire, the place and roles of catalytic antibodies in autoimmune pathologies. Synthesis and exploitation of antibodies libraries displayed on phage surface. Selection of specific ligands against identified target with regulation, detection or quantification applications. Antibodies engineering.

 

Skills

Cloning technics (PCR, RACE-PCR, mutagenesis, transformations…). Biochemical technics (enzymology, ELISA, Western Blots, Competition tests, SPR, recombinant proteins expression, refolding, cell culture…)

 

Roles and responsabilities

Supervision of students (PhD, masters, engineers). Project leader or member in various scientific programs (Sorbonne Université, FEDER, ANR, La Ligue contre le Cancer,…). Teaching in engineers school (UTC) and doctoral school. Courses leader (“Metabolism and cellular physiology”, course for L3 students, and “Life science for the Engineer” for L1 to L2 students).

International relationships Coordinator for the Biological Engineering Department.

 

General information

Since 2006, senior lecturer (section CNU 64) at University of Technology of Compiègne.

HDR (2013) “Understanding catalytic antibodies origin : Genetic analysis and Engineering”. PhD defensed in 2004 “biochemical and molecular characterization of an antiidiotypic antibody displaying a betalactamase like activity”.

 

Articles

Shahsavarian M., Chaaya N., Costa N., Boquet D., Atkinson A., Offmann B., Kaveri Sv., Lacroix-desmazes S., Friboulet A., Avalle B. & Padiolleau-lefevre S. Multi-target selection of catalytic antibodies wih . FEBS J.2017, 284,634-653.
     ABSTRACT: ??lactamase enzymes responsible for bacterial resistance to antibiotics are among the most important health threats to the human population today. Understanding the increasingly vast structural motifs responsible for the catalytic mechanism of ??lactamases will help improve the future design of new generation antibiotics and mechanism?based inhibitors of these enzymes. Here we report the construction of a large murine single chain fragment variable (scFv) phage display library of size 2.7 ? 109 with extended diversity by combining different mouse models. We have used two molecularly different inhibitors of the R?TEM ??lactamase as targets for selection of catalytic antibodies with ??lactamase activity. This novel methodology has led to the isolation of five antibody fragments, which are all capable of hydrolyzing the ??lactam ring. Structural modeling of the selected scFv has revealed the presence of different motifs in each of the antibody fragments potentially responsible for their catalytic activity. Our results confirm (a) the validity of using our two target inhibitors for the in vitro selection of catalytic antibodies endowed with ??lactamase activity, and (b) the plasticity of the ??lactamase active site responsible for the wide resistance of these enzymes to clinically available inhibitors and antibiotics.

Mahendra A., Peyron I., Thaunat O., Dollinger C., Gilardin L., Sharma M., Wootla B., Rao D. N., Padiolleau-lefevre S., Boquet D., More A., Varadarajan N., Kaveri S. V., Legendre C. & Lacroix-desmazes S. Generation of catalytic antibodies is an intrinsic property of an individual's immune system: a study on a large cohort of renal transplant patients.. J. Immunol.2016, 196,4075-4081.
 

Padiolleau-lefevre S., Ben Naya R., Shahsavarian M., Friboulet A. & Avalle B. Catalytic antibodies and their applications in biotechnology: State of the art.. Biotechnol. Lett.2014, 36,1369-1379.
 

Shahsavarian M., Le Minoux D., Matti K. M., Kaveri S., Lacroix-desmazes S., Boquet D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Exploitation of rolling circle amplification for the construction of large phafe display antibody libraries.. J. Immunol. Methods2014, 407,26-34.
 

Ben Naya R., Matti K., Guellier A., Matagne A., Boquet D., Thomas D., Friboulet A., Avalle B. & Padiolleau-lefevre S. Efficient refolding of a recombinant abzyme: Structural and catalytic characterizations.. Appl. Microbiol. Biotechnol.2013, 97,7721-7731.
 

Mahendra A., Peyron I., Dollinger C., Gilardin L., Sharma M., Wootla B., Padiolleau-lefevre S., Friboulet A., Boquet D., Legendre C., Kaveri S., Thaunat O. & Lacroix-desmazes S. IVIg treatment reduces catalytic antibody titers of renal transplanted patients.. PloS One2013, 8,e70731.
 

Mahendra A., Padiolleau-lefevre S., Kaveri S. & Lacroix-desmazes S. Do proteolytic antibodies complete the panopply of the autoimmune response in acquired haemophilia A?. Br. J. Haematol.2012, 156,3-12.
 

Le Minoux D., Mahendra A., Lacroix-desmazes S., Limnios N., Friboulet A., Avalle B., Boquet D., Kaveri S. & Padiolleau-lefevre S. A novel molecular analysis of genes encoding catalytic antibodies.. Mol. Immunol.2012, 50,160-168.
 

Phichith D., Bun S., Padiolleau-lefevre S., Guellier A., Banh S., Gallieni M., Frere J. M., Thomas D., Friboulet A. & Avalle B. Novel peptide inhibiting both TEM-1 beta-lactamase and penicillin-binding proteins.. FEBS J.2010, 277,4965-4972.
 

Essono S., Clement G., Padiolleau-lefevre S., Creminon C., Grassi J. & Boquet D. Peptide mass assisted antibody cloning strategy for accurate characterization of potential therapeutic monoclonal antibodies against neurodegenerative diseases.. Prot. Eng. Des. Select.2010, 23,203-210.
 

Phichith D., Bun S., Padiolleau-lefevre S., Banh S., Thomas D., Friboulet A. & Avalle B. Mutational and inhibitory analysis of a catalytic antibody. Implication for drug discovery.. Mol. Immunol.2009, 47,348-356.
 

Padiolleau-lefevre S., Debat H., Phichith D., Thomas D., Friboulet A. & Avalle B. Expression of a functional scFv fragment of an anti-idiotypic antibody with a ß-lactam hydrolytic activity. . Immunol. Letters2006, 103,39-44.
 

Patent

B. Avalle-bihan, A. Friboulet, H. Isber, C. Loussouarn, S. Padiolleau-lefèvre. Inhibiteurs de STAT5 et utilisation de ceux-ci. 2014, ,.
 

Albrecht ROSCHER

MC UPJV

            Contact Albrecht Roscher

 

 

 

Articles

Ramsay A., Fliniaux O., Quero A., Molinie R., Demailly H., Hano C., Paetz C., Roscher A., Grand E., Kovensky J., Schneider B. & Mesnard F. Kinetics of the incorporation of the main phenolic compounds into the lignan macromolecule during flaxseed development.. Food Chem.2017, 217,1-8.
 

Ramsay A., Fliniaux O., Fang J., Molinie R., Roscher A., Grand E., Guillot X., Kovensky J., Fliniaux M. A., Schneider B. & Mesnard F. Development of an NMR metabolomics-based tool for selection of flaxseed varieties.. Metabolomics2014, 10,1258-1267.
 

Koubaa M., Thomasset B. & Roscher A. Quantifying 13C-label in free sugars and starch by GC-MS.. Methods Molec. Biol.2014, 1090,121-130.
 

Roscher A., Troufflard S. & Taghki A. I. In vivo NMR for (13)C metabolic flux analysis.. Methods Molec. Biol.2014, 1090,143-152.
 

Fliniaux O., Corbin C., Ramsay A., Renouard S., Beejmohun V., Doussot J., Falguieres A., Ferroud C., Lamblin F., Laine E., Roscher A., Grand E., Mesnard F. & Hano C. Microwave-assisted extraction of herbacetin diglucoside from flax (Linum usitatissimum L.) seed cakes and its quantification using RP-HPLC-UV system.. Molecules2014, 19,3025-3037.
 

Fang J., Ramsay A., Paetz C., Tatsis E. C., Renouard S., Hano C., Grand E., Fliniaux O., Roscher A., Mesnard F. & Schneider B. Concentration kinetics of secoisolariciresinol diglucoside and its biosynthetic precursor coniferin in developing flaxseed.. Phytochem. Anal.2013, 24,41-46
  

Koubaa M., Mghaieth S., Thomasset B. & Roscher A. Gas chromatography-mass spectrometry analysis of 13C labeling in sugars for metabolic flux analysis.. Anal. Biochem.2012, 425,183-188.
 

Labboun S., Terce-laforgue T., Roscher A., Bedu M., Restivo F. M., Velanis C. N., Skopelitis D. S., Moshou P. N., Roubelakis K. A., Suzuki A. & Hirel B. Resolving the role of plant glutamate dehydrogenase: I. In vivo real time nuclear magnetic resonance spectroscopy experiments.. Plant Cell Physiol.2009, 50,17761-1773.
 

Bartholomeusz T. A., Molinie R., Mesnard F., Robins R. J. & Roscher A. Optimisation of 1D and 2D in vitro 1H NMR to study tropane alkaloid metabolism in Pseudomonas.. C.R. Chimie2008, 11,457-464.
 

Troufflard S., Roscher A., Thomasset B., Barbotin J. N., Rawsthorne S. & Portais J. C. In vivo (13)C NMR determines metabolic fluxes and steady state in linseed embryos.. Phytochem.2007, 68,, 2341-2350.
 

Robins R. J., Molinie R., Kwiecien R. A., Paneth P., Lebreton J., Bartholomeusz T. A., Roscher A., Drager B. & Mesnard F. Progress in understanding the N-demethylation of alkaloids by exploiting isotopic techniques. . Phytochem. Rev.2007, 6,51-63.
 

Bartholomeusz T. A., Mesnard F., Felpin F. X., Lebreton J., Robins R. J. & Roscher A. Optimised NMR detection of 13C-2H double labelling in small molecules. . C.R. Chimie2006, 9,514-519.
 

Houyou N., Pau-roblot C. & Roscher A. 15N relaxation and quantification of 15N labelled metabolites in cell extracts. . C.R. Chimie2006, 9,320-524.
 

Yannick ROSSEZ

CR CNRS - Co-Animator Theme PMB

             Contact Yannick Rossez

 

Research

Member of the theme “Plant Metabolism and Bioresources”.

Specialised in host-pathogen interaction and molecular characterisation by mass spectrometry, my interdisciplinary research is focused on bacterial adherence on plant and animal tissues. What I have done up till now is elucidating complex questions regarding bacterial adhesion and metabolic adaptation. For exemple, I have characterised the ligands of three different bacterial adhesins (LabA from Helicobacter pylori, ECP and flagella from E. coli). I have deciphered the pattern of glycosylation of H. pylori protein target.

 

Skills

Biochemistry and cellular biology: electrophoretic techniques (SDS-PAGE, agarose), western blotting, phage display, immunohistochemistry, chromatographic techniques (HPLC, FPLC, GC, gel permeation), animal experimentation, cell culture (stimulation and transfection), ELISA development, molecular biology.

Structural biology: glycomics, lipidomics, metabolomics, mass spectrometry techniques: MALDI-TOF/TOF and ESI-MS/MS (Q-TOF).

 

Roles and responsabilities

Since 2016, co-leader to one of the two theme in the GEC laboratory (regrouping 13 researchers and 5 technical staffs).

 

General information

2008-2011: PhD University of Technology of Lille 1, UGSF. Mentors: Jean-Claude Michalski and Catherine Robbe-Masselot.

My first key step in academic research, where I learned about mass spectrometry (MS) and how to identify complex molecules after fragmentation (MS/MS). The subject of my dissertation was mucin glycosylation in human stomach and the implication of O-glycans in Helicobacter pylori adhesion to the gastric mucosa. In parallel, I worked on the development of new peptides against the human gastric mucin MUC5AC, linked with contrast agents or fluorescein. MUC5AC is secreted in the colonic mucus of cancer patients and is a specific marker of precancerous lesions. Then, the purpose of this work was to develop an early diagnostic tool of colorectal cancer (Funded by Guerbet: A French pharmaceutical industry and the Nord Pas-de-Calais region).

2012-2014: Research assistant in Microbiology James Hutton Institute (ex: SCRI) (Scotland)/Mentor: Nicola Holden

I chose to go to Scotland for a postdoctoral position to stay in the exciting field of host-pathogen interaction in the unexplored area of human pathogens persistence on plants and expand my skills in molecular biology and microbiology. The work was focused on the molecular mechanisms underpinning foodborne outbreak associated pathogens with plants. We described, for the first time, how human pathogens could use plants as secondary host to target cattle or human.

2014-2016: Research assistant in plant metabolomics University of Technology of Compiegne (GEC laboratory)/SAS PIVERT

I decided to come back to France for this second postdoctoral positon to extend my knowledge on metabolomics and lipidomics. In this project, I explored the mechanism used by plants to distinguish usual and non-usual fatty acids on phospholipids (membrane lipids) as intermediates.

2016- Present: Junior CNRS researcher in host-bacteria Interaction University of Technology of Compiegne (GEC laboratory)/CNRS

I am currently working on bacterial flagella adhesion on plant and mammal cells. Plasma membrane lipids and cytoskeletal proteins are directly involved in flagellar molecular recognition to be stuck on host cell surfaces. At the same time, I am focusing on the metabolic cost for Escherichia coli O157:H7 to adapt to environmental conditions (outside animal reservoir) like temperature.

 

Personal interests

Tarantulas breeding and study. Molecular identification and phylogenetical analysis of Southeast Asia tarantula species. Interest in gardening with a focus on cold hardy bamboos.

 

 

Articles

Fahs Z., Rossez Y., Guénin S., Gutierrez L., Thomasset B., & Perrin, Y. Cloning and molecular characterization of three lysophosphatidic acid acyltransferases expressed in flax seeds. Plant Science2019, 280,41-50.
 

Nguyen P. J., Rippa S., Rossez Y. & Perriny. Acylcarnitines participate in developmental processes associated to lipid metabolism in plants.. Planta2016, 243,1011-1022.
 

Claire ROSSI

PR UTC - Manager Food Science Platform

          Contact Claire Rossi

 

Research

I’m working at the interface between the theme “Plant Metabolism and Bioresources” and “Biomimicry and Biomolecular Recognition”. My activities cover both fundamental and applied research.

Concerning fundamental research activities, I am particularly interested in studying the biological mode of action and health potential of phytochemicals found in food. My research deals with the development of sensors and the study of molecular interactions. It lies more particularly in the interactions occurring at the cell membrane interface. I developed the first in vitro systems ever reported for studying the protein translocation across biomimetic cell membranes as well as for the transmembrane protein dimerization and their consecutive activation.

Concerning applied research activities, I developed and lead a technology development and transfer platform in food science. The knowledge gained in nutritional biochemistry is applied for developing prototypes of innovative food products for companies. I built a strong experience in the nutritional optimisation of food products and in providing a healthy and bioactive added-value to food products. In 2018, a team of students that I led won the gold trophy of the competition Ecotrophelia France and the special price for the best innovation at Ecotrophelia Europe.
Skills

Characterization of molecular interactions; Study of the drug or phytochemical interactions with cells; Study of the bioactive properties of phytochemicals; Development and application of biosensors; Development of cell membrane models.

Food science; Food formulation; Food biochemistry; Nutritional optimization; Development of innovative food prototypes.
Roles and responsabilities

  • Since 2017: Elected member of the board of directors of the University of Technology de Compiègne. Alliance Sorbonne University.
  • Since 2015: Head of the Food Science platform. Innovation Center. UTC.
  • Since 2012: Head of the Major Innovation in Agrifood. Specialty of engineering diploma. Department of biological engineering, UTC.
  • Since 2013: Coordinator of the organic chemistry teaching module. UTC.
  • 2009-2017: Elected member of the Scientific Board of the Department of Biological Engineering CNRS, UTC.
  • Since 2007: Coordinator of the food formulation teaching module. UTC.

 

General information

  • Since Sept. 2016: Professor. Laboratory of cell and enzyme engineering CNRS UMR 7025. Department of biological engineering, University of Technology of Compiegne (UTC).
  • 2014-2016: Associate professor. CNRS UMR 7025, UTC.
  • April 2014: ‘Habilitation à diriger des recherches’ (Habilitation to conduct researches). UTC.
  • 2007-2014: Assistant professor. CNRS UMR 7025, UTC.
  • 2006-2007: Postdoctoral Research Associate. Max Planck Institute for Polymer Research, Mainz, Germany.
  • 2002-2006: PhD in biochemistry and biophysics. CNRS UMR 7025, UTC.
  • September 2002: M.Sc. in biochemistry. National High School of Chemistry of Toulouse ENSIACET, INP.
  • August 2002:Engineer diploma in organic chemistry. National High School of Chemistry of Toulouse, ENSIACET, INP.

 

Personal interests

  • Consulting in food formulation and in experimental design
  • Scientific advising for spin-off companies
  • Competitions in food innovation
  • Conduction of  public workshops devoted to the application of nutrition and food science knowledge
  • Writer for the website www.lanutrition.fr

 

 

Articles

Veneziano R., Rossi C., Chenal A., Brenner C., Ladant D., Chopineau J. Synthesis and characterization of tethered lipid assemblies for membrane protein reconstitution. Biointerphases2017, 12,04E301.
     ABSTRACT: Biological membranes and their related molecular mechanisms are essential for all living organisms. Membranes host numerous proteins and are responsible for the exchange of molecules and ions, cell signaling, and cell compartmentation. Indeed, the plasma membrane delimits the intracellular compartment from the extracellular environment and intracellular membranes. Biological membranes also play a major role in metabolism regulation and cellular physiology (e.g., mitochondrial membranes). The elaboration of membrane based biomimetic systems allows us to reconstitute and investigate, in controlled conditions, biological events occurring at the membrane interface. A whole variety of model membrane systems have been developed in the last few decades. Among these models, supported membranes were developed on various hydrophilic supports. The use of solid supports enables the direct use of surface sensitive techniques (e.g., surface plasmon resonance, quartz crystal microbalance, and atomic force microscopy) to monitor and quantify events occurring at the membrane surface. Tethered bilayer membranes (tBLMs) could be considered as an achievement of the first solid supported membranes described by the McConnell group. Tethered bilayers on solid supports were designed to delimit an inside compartment from an outside one. They were used for measuring interactions with ligands or incorporating large membrane proteins or complexes without interference with the support. In this context, the authors developed an easy concept of versatile tBLMs assembled on amino coated substrates that are formed upon the vesicle fusion rupture process applicable to protein-free vesicles as well as proteoliposomes. The phospholipid bilayer (natural or synthetic lipids) incorporated 5% of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-poly ethylene glycol-N-hydroxy succinimide to ensure the anchorage of the bilayer to the amino coated surface. The conditions for the formation of tBLMs on amino-coated gold and glass were optimized for protein-free vesicles. This biomimetic membrane delimits an inside

Hodrogue A., Trecherel E., Cornu M., Darwiche W., Mansour A., Ait-mohand K., Verissimo T., Gomila C., Schembri C., Da Nascimento S., Elboutachfait R., Boulier A., Lorne E., Courtois J., Petit E., Toumieux S., Kovensky J., Sonnet P., Massy Z. A., Kamel S. Rossi C., Ausseil J. Oligogalacturonic acid inhibits vascular calcification by two mechanisms: inhibition of VSMC osteogenic conversion and interaction with collagen. Arteriosclerosis, Thrombosis, and Vascular Biology2017, 37,1391-1401.
 

Nestora S., Merlier F., Prost E., Haupt K., Rossi C. & Tse Sum Bui B. Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer.. J. Chromatogr. A2016, 1465,47-54.
 

Xu J. J., Ambrosini S., Tamahkar E., Rossi C., Haupt K. & Tse Sum Bui B. Toward a universal method for preparing molecularly imprinted polymer nanoparticles with antibody-like affinity for proteins.. Biomacromolecules2016, 17,345-353.
 

Starok M., Preira P., Vayssade M., Haupt K., Salome L. & Rossi C. EGFR inhibition by curcumin in cancer cells: a dual mode of action.. Biomacromolecules2015, 16,1634-1642.
 

Nowacki L., Follet J., Vayssade M., Vigneron P., Cambay F., Egles C. & Rossi C. Real-time QCM-D monitoring of cancer cell death events in a dynamic context.. Biosens. Bioelectron.2015, 64,469-476.
 

Fabre G., Bayach I., Berka K., Paloncyova M., Starok M., Rossi C., Duroux J. L., Otyepka M. & Trouillas P. Synergism of antioxidant action of vitamins E and Quercetin is related to formation of molecular associations in biomembranes.. Chem. Commun.2015, 51,7713-7716.
 

Nowacki L., Vigneron P., Rotellini L., Cazzola H., Merlier F., Prost E., Ralanairina R., Gadonna J. P., Rossi C. & Vayssade M. Betanin-enriched red beetroot (Beta vulgaris L.) extract induces apoptosis and autophagic cell death in MCF-7 cells.. Phytother. Res.2015, 29,1964-1973.
 

Vidal G., Blanchi T., Mieszawska A. J., Calabrese R., Rossi C., Vigneron P., Duval J. L., Kaplan D. L. & Egles C. Enhanced cellular adhesion on titanium by silk functionalized with titanium binding and RGD peptides.. Acta Biomater.2013, 9,4935-4943.
 

Crosson C. & Rossi C. Quartz crystal microbalance immunosensor for the quantification of Immunoglobulin G in bovine milk.. Biosens. Bioelectron.2013, 42,453-459.
 

Veneziano R., Rossi C., Chenal A., Devoiselle J. M., Ladant D. & Chopineau J. Bordetella pertussis Adenylate Cyclase Toxin Translocation across a Tethered Lipid Bilayer.. Proc. Natl. Acad. Sci. USA2013, 110,20473-20478.
 

Doumiati S., Haupt K. & Rossi C. Autophosphorylation activation and inhibition by curcumin of the epidermal growth factor receptor reconstituted in liposomes.. J. Mol. Recognit.2012, 25,623-629.
 

Rossi C., Doumiati S., Lazzarelli C., Davi M., Meddar F., Ladant D. & Chopineau J. A tethered bilayer assembled on top of immobilized calmodulin to mimic cellular compartmentalization.. PloS One2011, 6,e19101.
 

Crosson C., Thomas D. & Rossi C. Quantification of immunoglobulin G in bovine and caprine milk using a surface plasmon resonance–based immunosensor.. J. Agr. Food Chem.2010, 58,3259-3264.
 

Eid M., Rippa S., Castano S., Desbat B., Chopineau J. Rossi C. & Beven L. Exploring the membrane mechanism of the bioactive peptaibol ampullosporin A using lipid monolayers and supported biomimetic membranes. J. Biophys.2010, 2010,Art.ID 179641.
 

Vockenroth I. K., Rossi C., Raza Sha M. & Koper I. Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques.. Biointerphases2009, 4,19-26.
 

Rossi C. & Haupt K Application of the Doehlert experimental design to molecularly imprinted polymers: surface response optimization of specific template recognition as a function of the type and degree of cross-linking.. Anal. Bioanal. Chem.2007, 389,455-460.
 

Rossi C. & Chopineau J. Biomimetic tethered lipid membranes designed for membrane-protein interaction studies.. Eur. Biophys. J.2007, 36,955-965.
 

Rossi C., Briand E., Parot P., Odorico M. & Chopineau J. Surface response methodology for the study of supported membrane formation.. J. Phys. Chem. B2007, 111,7567-7576.
 

Deniaud A., Rossi C., Berquand A., Homand J., Campagna S., Knoll W., Brenner C. & Chopineau J. Voltage-dependent anion channel transports calcium ions through biomimetic membranes.. Langmuir2007, 23,3898-3905.
 

BOOK

Maximilien J., Beyazit S., Rossi C., Haupt K. & Tse Sum Bui B Nanoparticles in biomedical applications. Measuring Biological impacts of nanomaterials2016, Springer International Publishing, Switzerland,pp. 177-210
  

Catherine SARAZIN

PR UPJV - Deputy director

            Contact Catherine Sarazin

 

 

 

Articles

Monnier N., Furlan A-l., Buchoux S., Deleu M., Dauchez M., Rippa S., Sarazin C. Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies" has been published in IJMS as part of the Special Issue SAR "Out of the Box. Int. J. Mol. Sci.2019, 20,1009.
 

Monnier M., Furlan A., Botcazon C., Dahi A., Mongelard G., Cordelier S., Clement C., Dorey S., Sarazin C., Rippa S. Rhamnolipids from Pseudomonas aeruginosa are elicitors triggering Brassica napus protection against Botrytis cinerea without physiological disorders. Frontiers in Plant Science2018, 1778,101-124.
     ABSTRACT: Rhamnolipids (RLs) are amphiphilic molecules naturally produced by some bacteria with a large range of biological activities. Although some studies report their potential interest in plant protection, evaluation of their effects and efficiency on annual crops of worldwide agronomic interest is lacking. The main objective of this work was to investigate their elicitor and protective activities on rapeseed crop species while evaluating their physiological effects. Here we report that RLs from Pseudomonas aeruginosa secretome trigger an effective protection of Brassica napus foliar tissues toward the fungus Botrytis cinerea involving the combination of plant defense activation and direct antimicrobial properties. We demonstrated their ability to activate canonical B. napus defense responses including reactive oxygen species production, expression of defense genes, along with callose deposits and stomatal closure as efficient physical protections. In addition, microscopic cell death observations and electrolyte leakage measurements indicated that RLs trigger a hypersensitive response-like defense in this plant. We also showed that foliar spray applications of RLs do not induce deleterious physiological consequences on plant growth or chlorophyll content and that RL protective properties are efficient on several grown cultivars of rapeseed. To our knowledge, this is the first report of RLs as an elicitor that suppresses fungal disease on tissues of an annual crop species under greenhouse conditions. Our results highlight the dual mode of action of these molecules exhibiting plant protection activation and antifungal activities and demonstrate their potential for crop cultures as environmental-friendly biocontrol solution.

Husson E., Auxenfans T., Herbaut M., Baralle M., Lambertyn V., Rakotoarivonina H., Remond C. & Sarazin C. Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases.. Bioresource Technol.2018, 251,280-287.
     ABSTRACT: Sequential and simultaneous strategies for fractioning wheat straw were developed in combining 1-ethyl-3-methyl imidazolium acetate [C2mim][OAc], endo-xylanases from Thermobacillus xylanilyticus and commercial cellulases. After [C2mim][OAc]-pretreatment, hydrolysis catalyzed by endo-xylanases of wheat straw led to efficient xylose production with very competitive yield (97.6?

Mehmood N., Alayoubi R., Husson E., Jacquard C., Buchs J., Sarazin C. & Gosselin I. Kluyveromyces marxianus, an attractive yeast for ethanolic fermentation in the presence of imidazolium ionic liquids.. Int. J. Molec. Sci.2018, 19,887.
     ABSTRACT: Imidazolium ionic liquids (ILs) are promising solvents for lignocellulosic biomass (LCB) pretreatment and allow the achievement of higher ethanolic yields after enzymatic hydrolysis and ethanolic fermentation. However, residual ILs entrapped in pretreated biomass are often toxic for fermentative microorganisms, but interaction mechanisms between ILs and cells are still unknown. Here we studied the effects of 1-ethyl-3-methylimidazolium acetate [Emim][OAc] and 1-ethyl-3-methylimidazolium methylphosphonate [Emim][MeO(H)PO2] on Kluyveromyces marxianus, a thermotolerant ethanologenic yeast. Morphological impacts induced by ILs on K. marxianus were characterized by Scanning Electron Microscopy analysis and showed wrinkled, softened, and holed shapes. In Yeast-Malt-Dextrose (YMD) medium, K. marxianus tolerated IL additions up to 2% for [Emim][OAc] and 6% for [Emim][MeO(H)PO2]. Below these thresholds, some IL concentrations enhanced ethanolic yields up to +34% by switching the metabolic status from respiratory to fermentative. Finally, K. marxianus fermentation was applied on several substrates pretreated with [Emim][OAc] or [Emim][MeO(H)PO2] and enzymatically hydrolyzed: a model long fiber cellulose and two industrial LCBs, softwood (spruce) and hardwood (oak) sawdusts. The maximum ethanolic yields obtained were 1.8 to 3.9 times higher when substrates were pretreated with imidazolium ILs. Therefore K. marxianus is an interesting fermentative yeast in a second-generation bioethanol process implying IL pretreatment

Brahim M., Checa Fernandez B. L., Regnier O., Bousseta N., Grimi N., Sarazin C., Husson E., Vorobiev E., Brosse N. Impact of ultrasounds and high voltage electrical discharges on physico-chemical properties of rapeseed straw’s lignin and pulps. Bioresource Technology2017, 237,42308
  

Auxenfans T., Husson E. & Sarazin C. Simultaneous pretreatment and enzymatic saccharification of (ligno)celluloses in aqueous-ionic liquid media: a compromise.. Biochem. Eng. J.2017, 117,77-86.
     ABSTRACT: In view of decreasing the amount of IL to achieve efficient simultaneous pretreatment and saccharification, a comprehensive study was undertaken. Different types of lignocellulosic biomasses were investigated in various enzymatic aqueous-IL systems including 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) or 1-ethyl-3-methylimidazolium methylphosphonate ([C2mim][MeO(H)PO2]). To better understand how 10% (v/v) of IL in the reaction medium led to the highest yields of glucose without fractionation from sawdust, distinct cellulosic models were then used as substrates. Kinetic studies demonstrated that both ILs affect synergistic action of cellulolytic enzymes depending on both constitutive anion and cellulosic substrate. Concentrations above 10% v/v of ILs deactivated cellulase even on highly digestible model substrates. ? and ? Kamlet-Taft parameters constituted more physicochemical pertinent indicators than apparent pH value to investigate effects of IL on cellulase performances. Fine description of these effects was proposed onto individual EG, CBH and BG. [C2mim][MeO(H)PO2] was demonstrated a better compatible IL for enzymes up to a concentration of 30% (v/v). The efficiency of simultaneous pretreatment and saccharification was governed by a compromise between better substrate accessibility and enzyme deactivation.

Mehmood N., Husson E., Jacquard C., Wewetzer S., Buchs J., Sarazin C. & Gosselin I. Impact of two ionic liquids, 1-ethyl-3-methylimidazolium acetate and 1-ethyl-3-methylimidazolium methylphosphonate, on Saccharomyces cerevisiae: metabolic, physiologic, and morphological investigations.. Biotechnol. Biofuels 2015, 8,n.17.
 

Bonnet V., Gervaise C., Djedaini-pilard F., Furlan A. & Sarazin C. Cyclodextrin nanoassemblies: a promising tool for drug delivery.. Drug. Discov. Today2015, 20,1120-1126.
 

Hulin L., Husson E., Bonnet J. P., Stevanovic T. & Sarazin C. Enzymatic transesterification of kraft lignin with long acyl chains in ionic liquids.. Molecules2015, 20,16334-16353.
 

Bompart M., Goto A., Wattraint O., Sarazin C., Tsujii Y., Gonzato C. & Haupt K. Molecularly imprinted polymers by reversible chain transfer catalyzed polymerization.. Polymer2015, 78,31-36.
 

Auxenfans T., Buchoux S., Husson E. & Sarazin C. Efficient enzymatic saccharification of Miscanthus: energy-saving by combinig dilute acid and ionic liquid pretreatments.. Biomass Bioenerg.2014, 62,82-92.
 

Auxenfans T., Buchoux S., Larcher T., Husson G., Husson E. & Sarazin C. Enzymatic saccharification and structural properties of industrial wood sawdust: recycled ionic liquids pretreatments.. Energ. Convers. Manage.2014, 88,1094-1103.
 

Gervaise C., Bonnet V., Nolay F., Cezard C., Stasik I., Sarazin C. & Djedaini-pilard F. Diesterification of 3-[(ß-cyclodextrinyl)succinamido]propane-1,2-diol catalyzed by lipase: Diastereoselectivity or tridimensional substrate specificity?. Eur. J. Org. Chem.2014, 2014,6200-6209.
 

Wattraint O., Saadallah I., Silva-peres V., Sonnet P. & Sarazin C. Influence of the insertion of a cationic peptide on the size and shape of nanoliposomes: a light scattering investigation.. Int. J. Pharm.2013, 454,621-624.
 

Bonnet V., Favrelle A., Aubry F., Sarazin C. & Djedaini-pilard F. Solvent-free chemo-enzymatic synthesis of fatty acyl-bcyclodextrin.. J. Incl. Phenom. Macrocycl. Chem.2013, 77,155-161.
 

Gervaise C., Bonnet V., Wattraint O., Aubry F., Sarazin C., Jaffres P. A. & Djedaini-pilard F. Synthesis of lipophosphoramidyl-cyclodextrins and their supramolecular properties.. Biochimie2012, 94,66-74.
 

Auxenfans T., Buchoux S., Djellab K., Avondo C., Husson E. & Sarazin C. Mild pretreatment and enzymatic saccharification of cellulose with recycled ionic liquid towards one-batch process.. Carbohydr. Polym.2012, 90,805-813.
 

Gervaise C., Bonnet V., Sarazin C. & Djedaini-pilard F. Synthesis of glycerolipidyl derivatives of permethylated b-cyclodextrin as potential nanovectors.. New J. Chem.2012, 36,2417-2424.
 

Husson E., Buchoux S., Avondo C., Cailleu D., Djellab K., Gosselin I., Wattraint O. & Sarazin C. Enzymatic hydrolysis of ionic liquid-pretreated celluloses: contribution of CP-MAS, 13C NMR and SEM.. Bioresource Technol.2011, 102,7335-7342.
 

Bonnet V., Gervaise C., Favrelle A., Sarazin C. & Djedaini-pilard F. Enzymatic catalysis in presence of cyclodextrins.. Curr. Org. Chem.2010, 14,1323-1336.
 

Favrelle A., Bonnet V., Avondo C., Aubry F., Djedaini-pilard F. & Sarazin C. Lipase-catalyzed synthesis and characterization of novel lipidyl-cyclodextrins in solvent free medium.. J. Mol. Catal. B.2010, 66,224-227.
 

Kouzayha A., Wattraint O. & Sarazin C. Interactions of two transmembrane peptides in supported lipid bilayers studied by a 31P and 15N MAOSS NMR strategy.. Biochimie2009, 91,774-778.
 

Kouzayha A., Nasir M. N., Buchet R., Wattraint O., Sarazin C. & Besson F. Conformational and interfacial analyses of K3A18K3 and alamethicin in model membranes.. J. Phys. Chem. B2009, 113,7012-7019.
 

Favrelle A., Bonnet V., Sarazin C. & Djedaini-pilard F. Synthesis of polyenyl derivatives of permethylated beta-cyclodextrin.. Tetrahedron Asym.2008, 19,2240-2245.
 

Favrelle A., Bonnet V., Sarazin C. & Djedaini-pilard F. Novel chemo-enzymatic access to amphiphilic cyclodextrins. . J. Incl. Phenom. Macrocycl. Chem.2007, 57,15-20.
 

Wattraint O. & Sarazin C. Static and MAS solid-state study of supported phospholipid bilayer cylindrically oriented. . C.R. Chimie2006, 9,408-412.
 

Patent

E. Husson, C. Sarazin, H. Ducatel, I. Murrieta-pazos, G. Ravot Procédé de traitement de la biomasse. 2017, ,.
 

Adrian TRONCOSO-PONCE

        Contact Adrian Troncoso-Ponce

Research

Member of the theme “Plant Metabolism and Bioresources”.

The “Chaire d’excellence junior, étude et valorisation du métabolisme végétal” is placed at the junction of the interests of the “Hauts-de-France” and the UTC in promoting Agro-resources and the improvement of the industrial landscape of the region. As such, the study of plant metabolism and its interaction with environmental factors is of great interest, particularly the scientific investigation of oilseed plants due to their economical significance for the region. Considering the objectives of the Chair, the scientific project has been designed in order to shed light on the plant lipid metabolism in relationship with the circadian and diurnal changes.

 

Skill

Plant science, biochemistry, molecular biology, transcriptomics, primary carbon metabolism, and lipid metabolism.

 

Roles and responsabilitie

With the aim of accomplishing the objectives of my Chair responsibilities, I actively participate in the training of UTC students.

Joint master degree between the UPJV and the UTC “Mention Chemistry”

I have created a course entitled “Applied microbiology, new concepts” (UE BG09). In this new course (20 hours in English) an update of the progress in cutting-edge microbiological research and applications (biotechnology, agriculture, medicine, etc) is provided to the students.

Based on my experience with plant metabolism I am co-responsible of the course “Valorisation de la biomasse et production biotechnologique de molecules d´intérêts” (UE BG10). Moreover, I participate in the course “Biocontrôle et néophytosanitaires” (UE BG08), where I teach 4 hours about metabolism and synthesis of defense molecules in plants.

Biological engineering (GB): Metabolism and cellular physiology (BL20)

Taking part in the training of new engineers is central to my objectives, especially considering the position of UTC one of France’s most highly ranked engineering schools. For that reason and based on my experience in enzymology, I have created a new practical lab course on the characterization of allosteric enzymes “Métabolisme, régulation de la glycolyse” that I am teaching myself (24 hours in total). This TP complements and illustrates the lectures on metabolic regulation. Furthermore, I am in charge of teaching another practical lab course, “Diffusion d’ions à travers une membrane semi-perméable” (24h in total), where students analyze the semipermeability of a membrane in order to understand the importance of ion transport through different compartments.

 

General information

My experience in plant metabolism started when I joined the Group of Genetics and Biochemistry of Seed Lipids (GGBSL, Seville, Spain) at the Spanish Scientific Research Higher Council (CSIC) in 2003. My Ph.D. research program focused on carbon primary metabolism in sunflower seeds and was carried out under the supervision of Prof. Garcés and Dr. Martinez-Force. During my Ph.D., I conducted a significant part of my work in collaboration with two leading laboratories in the field of plant metabolism: Dr. Rivoal’s group from the Plant Biology Research Institute (University of Montreal, Canada) and Dr. Kruger’s group from the Department of Plant Science of the Oxford University (UK). Upon completing my Ph.D. in 2008, I continued working on plant lipid metabolism as a post-doctoral researcher in the Plant Lipid Metabolism laboratory headed by Distinguished Prof. Ohlrogge at Michigan State University (MSU, USA). In this laboratory, I focused primarily on transcriptome analysis of oil crops. From 2010 to 2012, I served as a postdoctoral researcher in Dr. Mas’ group at the Center for Research in Agricultural Genomics (CRAG) in Barcelona (Spain), where I conducted circadian clock studies in Arabidopsis thaliana. From 2012 to 2014, I rejoined the GGBSL where I conducted post-doctorate research on the characterization of glycolytic enzymes in oilseeds (Seville, Spain). In 2014, I secured a Marie Curie Intra-European Fellowship. This grant allowed me to work for a period of two years in the group of Dr. Baud at Institute Jean-Pierre Bourgin (INRA, France) where my project was focused on transcriptional regulation of oil accumulation in oilseeds. In 2017 I obtained the position of Chair for the “study and valorization of plant metabolism” at the University of technology of Compiègne. The Chair is associated with the Enzyme and Cell Engineering Unit (GEC – UMR CNRS 7025).

 

Personal interests

Over the past few years, I have been interested on the past and present of humans, from paleoanthropology to modern history. My interest on human evolution evolved from my fascination for paleontology and the reciprocal influence of Life and geology throughout eons.

I am also interested on a different type of human interaction, “la boxe française”.

Brigitte THOMASSET

DR CNRS - Co-Animator Theme PMB

        Contact Brigitte Thomasset

 

 

Articles

Fahs Z., Rossez Y., Guénin S., Gutierrez L., Thomasset B., & Perrin, Y. Cloning and molecular characterization of three lysophosphatidic acid acyltransferases expressed in flax seeds. Plant Science2019, 280,41-50.
 

Tshabuse F., Farrant J. M., Humbert L., Moura D., Rainteau D., Espinasse C., Idrissi A., Merlier F., Acket S., Rafudeen M. S., Thomasset B. & Ruelland E. Glycerolipid analysis during desication and recovery of the resurrection plant Xerophyta humilis (Bak) Dur and Schinz.. Plant Cell Environ.2018, 41,533-547.
     ABSTRACT: Feelings in humans are mental states representing groups of physiological functions that usually have defined behavioural purposes. Feelings, being evolutionarily ancient, are thought to be coordinated in the brain stem of animals. One function of the brain is to prioritise between competing mental states and, thus, groups of physiological functions and in turn behaviour. Plants use groups of coordinated physiological activities to deal with defined environmental situations but currently have no known mental state to prioritise any order of response. Plants do have a nervous system based on action potentials transmitted along phloem conduits but which in addition, through anastomoses and other cross?links, forms a complex network. The emergent potential for this excitable network to form a mental state is unknown, but it might be used to distinguish between different and even contradictory signals to the individual plant and thus determine a priority of response. This plant nervous system stretches throughout the whole plant providing the potential for assessment in all parts and commensurate with its self?organising, phenotypically plastic behaviour. Plasticity may, in turn, depend heavily on the instructive capabilities of local bioelectric fields enabling both a degree of behavioural independence but influenced by the condition of the whole plant.

Acket S., Degournay A., Merlier F. & Thomasset B. Data documenting the comparison between the theoretically expected values of free sugars mass isotopomer composition with standards using GC-MS and LC-HRMS for Metabolic Flux Analysis.. Data in Brief2017, 12,108-112.
     ABSTRACT: The data presented in this article are related to the research article entitled

Hano C., Corbin C., Quero A., Rombaut N., Savoire R., Molinie R., Thomasset B., Mesnard F. & Laine E. The use of (+)-secoisolariciresinol extracted from the hulles as en effective protectant of linseed oil and its emulsion against oxidative damage. . Eur. J. Lipid Sci. Technol2017, 118,
  

Hano C., Corbin C., Drouet S., Quero A., Rombaut N., Savoire R., MoliniÉ R., Thomasset B., Mesnard F., LainÉ E. The lignan (+)-secoisolariciresinol extracted from flax hulls is an effective protectant of linseed oil and its emulsion against oxidative damage . Eur. J. Lipid Sci. Technol. 2017, 119,1600219.
     ABSTRACT: Secoisolariciresinol (SECO) is a natural antioxidant lignan accumulated in large amounts in the seedcoat of flax and retained in the flaxseed cake residue during linseed oil pressing. Here SECO was extracted and purified from flaxseed cake and assayed for its ability to prevent oxidation of linseed oil and an o/w emulsion containing linseed oil. For this purpose, an accelerated storage (Schaal oven) test was performed and SECO effectiveness was compared to that of two antioxidants commonly used in food and cosmetic products: ??tocopherol (??TOCO) and butylated hydroxyanisole (BHA). In our hands, SECO addition, ranging from 50 to 500??mole per kg oil, significantly decreased the production of both primary (conjugated dienes, CD) and secondary (thiobarbituric acid?reactive substances, TBARS) oxidation products. This study evidenced that SECO is an effective stabilizer of linseed oil and its o/w emulsion and this protective effect outperformed both the natural ??TOCO and the synthetic BHA antioxidants. In particular, SECO was the most effective in the protection of the o/w emulsion against secondary oxidation products, which makes it a potential alternative preservative for oily products in foods and cosmetics.

Quero A., Molinie R., Mathiron D., Thiombiano B., Fontaine J. F., Brancourt D., Van Wuytswinkel O., Petit E., Demailly H., Mongelard G., Pilard S., Thomasset B. & Mesnard F. Metabolite profiling in developing Camelina sativa seeds.. Metabolomics2016, 12,186-199.
 

Rombaut N., Savoire R., Thomasset B., Castello J. & Van Hecke E. Optimization of oil yield and oil total phenolic content during grape seed cold screw pressing.. Ind. Crops Prod.2015, 63,26-33.
 

Lazouk M. A., Savoire R., Kaddour A., Castello J., Lanoiselle J. L., Van Hecke E. & Thomasset B. Oilseeds sorption isoterms, mechanical properties and pressing.: Global view of water impact.. J. Food Eng.2015, 153,73-80.
 

Savoire R., Lazouk M. A., Van-hecke E., Roulard R., Tavernier R., Guillot X., Rhazi L., Petit E., Mesnard F. & Thomasset B. Environmental and varietal impact on linseed composition and on oil unidirectional expression process.. OCL2015, 22,D605.
 

Rombaut N., Savoire R., Thomasset B., Belliard T., Castello J., Van Hecke E. & Lanoiselle J. L. Grape seed oil extraction: interest of supercritical fluid extraction and gas assisted mechanical extraction for enhancing polyphenol co-extraction in oil.. C.R. Chimie2014, 17,284-292.
 

Koubaa M., Thomasset B. & Roscher A. Quantifying 13C-label in free sugars and starch by GC-MS.. Methods Molec. Biol.2014, 1090,121-130.
 

Koubaa M., Cocuron J. C., Thomasset B. & Alonso A. Highlighting the tricarboxylic acid cycle : liquid and gas chromatography-mass spectrometry analyses of 13C-labeled organic acids.. Anal. Biochem.2013, 436,151-159.
 

Chantreau M., Grec S., Gutierrez L., Dalmais M., Pineau C., Demailly H., Paysant-leroux C., Tavernier R., Trouve J. P., Chatterjee M., Guillot X., Brunaud V., Chabbert B., Van Wuytswinkel O., Bendahmane A., Thomasset B. & Hawkins S. PT-Flax (phenotyping and TILLing of flax): development of a flax (Linum usitatissimum L.) mutant population and TILLing platform for forward and reverse genetics.. BMC Plant Biol.2013, 13,159-174.
 

Fenart S., Chabi M., Gallina S., Huis R., Neutelings G., Riviere N., Thomasset B., Hawkins S. & Lucau-danila A. Intra-platform comparison of 25-mer and 60-mer oligonucleotide Nimblegen DNA microarrays.. BMC Res. Notes2013, 6,43.
 

Koubaa M., Mghaieth S., Thomasset B. & Roscher A. Gas chromatography-mass spectrometry analysis of 13C labeling in sugars for metabolic flux analysis.. Anal. Biochem.2012, 425,183-188.
 

Pilalis E., Chatziioannou A., Thomasset B. & Kolisis F. An In-silico compartmentalized, metabolic model of Brassica napus, enables the systemic study of regulatory aspects of plant central metabolism.. Biotechnol. Bioeng.2011, 108,1673-1682.
 

Fenart S., Ndong Y. P., Duarte J., Riviere N., Van Wuytswinkel O., Lucau A., Cariou E., Neutelings G., Gutierrez L., Chabbert B., Guillot X., Tavernier R., Hawkins S. & Thomasset B. Development and validation of a flax (Linum usitatissinum L.) gene expression oligo microarray.. BMC Genomics2010, 11,592.
 

Jousse C., Schiltz S., Fournez A., Guillot X., Thomasset B., Gougeon S., Bourgaud F. & Gontier E. Rapid, cost-effective screening of flax genotypes to identify desirable fatty acid compositions.. Electron. J. Plant Breed.2010, 1,1396-1404
  

Nlandu Mputu M., Rhazi L., Vasseur G., Vu T. D., Gontier E. & Thomasset B. Identification of a potential bottleneck in branched chain fatty acid incorporation into triacylglycerol for lipid biosynthesis in agronomic plants.. Biochimie2009, 91,703-710.
 

Lievre K., Tran T. L., Doerper S., Hehn A., Lacoste P., Thomasset B., Bourgaud F. & Gontier E. Agrobacterium-mediated transformation of Ruta graveciens L.. Methods Molec. Biol.2009, 547,235-248.
 

Caillot S., Rosiau E., Laplace C. & Thomasset B. Influence of light intensity and selection scheme on regeneration time of transgenic flax plants.. Plant Cell Rep.2009, 28,359-371.
 

Noizet M., Harrabi F., Vijayalakshmi M., Galbraith D., Thomas D. & Thomasset B. Targeted protein accumulation promoted by autoassembly and its recovery from plan cells.. Biotechnol. J.2008, 3,392-402.
 

Troufflard S., Roscher A., Thomasset B., Barbotin J. N., Rawsthorne S. & Portais J. C. In vivo (13)C NMR determines metabolic fluxes and steady state in linseed embryos.. Phytochem.2007, 68,, 2341-2350.
 

Gutierrez L., Conejero G., Castelain M., GuÉnin S., Verdeil J. L., Thomasset B. & Van Wuytswinkel O. Identification of new gene expression regulators specifically expressed during plant seed maturation.. J. Exp. Bot.2006, 57,1919-1932.
 

Patent

P. Carre, A. Lazouk, B. Thomasset, R. Savoir, L. Rhazi. Procédé de décorticage de graines. 2015, ,.
 

Olivier WATTRAINT

MC UPJV

Contact Olivier Wattraint

 

 

Articles

Maherani B., Wattraint O. Liposomal structure: A comparative study on light scattering and chromatography techniques. Journal of Dispersion Science and Technology2017, 38,1633-1639.
 

Bompart M., Goto A., Wattraint O., Sarazin C., Tsujii Y., Gonzato C. & Haupt K. Molecularly imprinted polymers by reversible chain transfer catalyzed polymerization.. Polymer2015, 78,31-36.
 

Wattraint O., Saadallah I., Silva-peres V., Sonnet P. & Sarazin C. Influence of the insertion of a cationic peptide on the size and shape of nanoliposomes: a light scattering investigation.. Int. J. Pharm.2013, 454,621-624.
 

Gervaise C., Bonnet V., Wattraint O., Aubry F., Sarazin C., Jaffres P. A. & Djedaini-pilard F. Synthesis of lipophosphoramidyl-cyclodextrins and their supramolecular properties.. Biochimie2012, 94,66-74.
 

Husson E., Buchoux S., Avondo C., Cailleu D., Djellab K., Gosselin I., Wattraint O. & Sarazin C. Enzymatic hydrolysis of ionic liquid-pretreated celluloses: contribution of CP-MAS, 13C NMR and SEM.. Bioresource Technol.2011, 102,7335-7342.
 

Kouzayha A., Wattraint O. & Sarazin C. Interactions of two transmembrane peptides in supported lipid bilayers studied by a 31P and 15N MAOSS NMR strategy.. Biochimie2009, 91,774-778.
 

Kouzayha A., Nasir M. N., Buchet R., Wattraint O., Sarazin C. & Besson F. Conformational and interfacial analyses of K3A18K3 and alamethicin in model membranes.. J. Phys. Chem. B2009, 113,7012-7019.
 

Wattraint O. & Sarazin C. Static and MAS solid-state study of supported phospholipid bilayer cylindrically oriented. . C.R. Chimie2006, 9,408-412.