Mass spectrometry

Supervisor: Franck MERLIER

Expertise and instrumentation in high and low resolution mass spectrometry for small molecules analysis .

Expertise:

  •  Fluxomics
    •  Isotope enrichment analysis by HPLC-QTOF
    •  Quantification of metabolites (organic acid, amino acids, sugars, …)
  • Lipidomics
    • Fatty Acids (FAME, picolynyl)
    • Phospholipids
  •  Qualitative analyzes
    • Identification of small molecules by GC-HRMS (GC Orbitrap) and HPLC-HRMS (LC-QTOF).
  •  Qualitative analyzes
    • Development of quantification methods by chromatography (liquid, gas or capillary) with detections by mass spectrometry (HRMS, MS / MS), FID, ECD (GC) or RI / Fluorescence / UV (HPLC).

  • Preparatory analyzes
    • Semi preparative and preprative HPLC mixing with RI / UV / FLD / MS detections
  • GPC / SEC analysis
    • Determination of the mass of macro molecules by aqueous or organic GPC.

Simple services or work of medium and long-term collaborations, methodological research with industry and industry.

Facilities:

Mass spectrometry

  • Agilent QTOF 6538

High resolution mass spectrometer (R = 40,000), mass accuracy less than 2ppm equipped with electrospray and APCI sources. The mass spectrometer can be used in coupling with Agilent 1290 HPLC with DAD or fluorescence detection, capillary electrophoresis and ion chromatography.

  •  Agilent QQQ 6460

Tandem mass spectrometer equipped with electrospray and APCI sources. The mass spectrometer can be used in conjunction with Agilent 1290 HPLC, capillary electrophoresis and ion chromatography or preparative Agilent 1260 HPLC with DAD detection.

  •  Thermo Scientific GC-Qexactive

High resolution mass spectrometer (R = 120,000), mass accuracy less than 2ppm equipped with electronic impact and chemical ionization sources (Methane and Ammonia) coupled with Thermo Gas Chromatography.

  • GC-Quantum Scientific Thermo

Tandem mass spectrometer equipped with electronic impact and chemical ionization sources (Methane and Ammonia) coupled with Thermo Gas Chromatography.

Liquid chromatography:

  • Agilent HPLC 1260

Analytical and semi-preparative HPLC chromatography chain with UV detections, Flurorescence, RI and Fractions collectors

  • Agilent 1260 infinity 2 HPLC

HPLC preparative chromatography chain with UV detections and Fractions collectors

  • Dionex HPLC

Analytical HPLC / GPC chromatography chain with UV, RI and MALLS detections.

Gas Chromatography:

The laboratory is equipped with 3 Shimadzu gas chromatography ovens 2010 equipped with FID detector but also with an electron capture detector (ECD).